Nils Rademacher

Publications of Nils Rademacher

• Identification of a novel CDKL5 exon and pathogenic mutations in patients with severe mental retardation, early-onset seizures and Rett-like features.

  Authors: Nils Rademacher, Melanie Hambrock, Ute Fischer, Bettina Moser, Berten Ceulemans, Wolfgang Lieb, Rainer Boor, Irina Stefanova, Gabriele Gillessen-Kaesbach, Charlotte Runge, Georg Christoph Korenke, Stefanie Spranger, Franco Laccone, Andreas Tzschach, Vera M Kalscheuer

  Neurogenetics. 02/2011; 12(2):165-7.

• TRPV1 acts as a synaptic protein and regulates vesicle recycling.

  Authors: Chandan Goswami, Nils Rademacher, Karl-Heinz Smalla, Vera Kalscheuer, Hans-Hilger Ropers, Eckart D Gundelfinger, Tim Hucho

  Journal of cell science. 06/2010; 123(Pt 12):2045-57.

  Electrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as theElectrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as the central nervous system, the questions remain whether TRPV1 is present in synaptic structures and whether it is involved in synaptic processes. In the present study we gathered evidence that TRPV1 can be detected in spines of cortical neurons, that it colocalizes with both pre- and postsynaptic proteins, and that it regulates spine morphology. Moreover, TRPV1 is also present in biochemically prepared synaptosomes endogenously. In F11 cells, a cell line derived from dorsal-root-ganglion neurons, TRPV1 is enriched in the tips of elongated filopodia and also at sites of cell-cell contact. In addition, we also detected TRPV1 in synaptic transport vesicles, and in transport packets within filopodia and neurites. Using FM4-64 dye, we demonstrate that recycling and/or fusion of these vesicles can be rapidly modulated by TRPV1 activation, leading to rapid reorganization of filopodial structure. These data suggest that TRPV1 is involved in processes such as neuronal network formation, synapse modulation and release of synaptic transmitters.

• TRPV1 acts as a synaptic protein and regulates vesicle recycling.

  Authors: Chandan Goswami, Nils Rademacher, Karl-Heinz Smalla, Vera M Kalscheuer, Hans-Hilger Ropers, Eckart D Gundelfinger, Tim Hucho

  Journal of Cell Science, v.123, 2045-2057 (2010).

  Electrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as theElectrophysiological studies demonstrate that transient receptor potential vanilloid subtype 1 (TRPV1) is involved in neuronal transmission. Although it is expressed in the peripheral as well as the central nervous system, the questions remain whether TRPV1 is present in synaptic structures and whether it is involved in synaptic processes. In the present study we gathered evidence that TRPV1 can be detected in spines of cortical neurons, that it colocalizes with both pre- and postsynaptic proteins, and that it regulates spine morphology. Moreover, TRPV1 is also present in biochemically prepared synaptosomes endogenously. In F11 cells, a cell line derived from dorsal-root-ganglion neurons, TRPV1 is enriched in the tips of elongated filopodia and also at sites of cell-cell contact. In addition, we also detected TRPV1 in synaptic transport vesicles, and in transport packets within filopodia and neurites. Using FM4-64 dye, we demonstrate that recycling and/or fusion of these vesicles can be rapidly modulated by TRPV1 activation, leading to rapid reorganization of filopodial structure. These data suggest that TRPV1 is involved in processes such as neuronal network formation, synapse modulation and release of synaptic transmitters.