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ABSTRACT: Pendred syndrome is one of the most common hereditary determined diseases in patients with syndromic sensorineural hearing impairment. Mutations in the SLC26A4 gene are a major cause of Pendred syndrome. However, Pendred syndrome is quite rare in China. This investigation aims to identify genetic cause of a Chinese family with Pendred syndrome.
Clinical and molecular evaluations were conducted in a Chinese family with Pendred syndrome.
A novel SLC26A4 c.662_663insG mutation was detected in compound heterozygosity with IVS7-2A>G. No FOXI1, KCNJ10 or GJB2 pathogenic mutation was found. The novel mutation c.662_663insG (p.G221) locates in SLC26A4 gene exon 6, and cause frameshift mutation on pendrin protein transmembrane domain five.
The compound heterozygosity of the novel c.662_663insG and IVS7-2A>G mutations in the SLC26A4 gene was considered to be the cause of Pendred syndrome in the proband. This study also supplemented the mutation spectrum of Pendred syndrome.
International journal of pediatric otorhinolaryngology 08/2012; 76(11):1633-6. · 0.85 Impact Factor
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ABSTRACT: OBJECTIVE: To explore the clinical effect and possible mechanism of macrolides on otitis media with effusion in children. METHODS: Children with otitis media with effusion were recruited and prescribed for macrolides according to a designed scheme, and followed up for 8-12 weeks. Middle ear effusion samples were collected from the participants to assess the presence of biofilm. RESULTS: Macrolides were found to have significant effect on the therapy of early stage otitis media with effusion for 88.7-92.5% of participants compared with 50.9-60.3% in control group after 8-12 weeks. Almost 72.1% of chronic otitis media with effusion patients recovered after an 8 weeks' course low dose macrolides prescription. Biofilm occurred in 30.8% (4/13) of middle ear effusion. CONCLUSIONS: The results indicate that macrolides are effective for otitis media with effusion in children. Bacterial biofilm may play an important role in the pathogenesis of otitis media with effusion.
Auris, nasus, larynx 06/2012; · 0.58 Impact Factor
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Weiping Wen,
Wenlong Liu,
Luo Zhang,
Jing Bai,
Yunping Fan,
Wentong Xia,
Qing Luo,
Jing Zheng,
Hongtian Wang,
Zuwang Li,
Jiahong Xia, Hongyan Jiang,
Zheng Liu,
Jianbo Shi,
Huabin Li,
Geng Xu
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ABSTRACT: Nasal polyps (NPs) are characterized by eosinophilic inflammation, which is generally considered sensitive to corticosteroid treatment.
We evaluated levels of neutrophilia in NPs and investigated whether increased neutrophilia in polyp tissue affected the response to corticosteroid treatment.
We studied 3 independent cross-sectional groups of patients with NPs. Levels of infiltration by different types of inflammatory cells were determined by using immunohistochemical analyses and compared with those seen in control nasal tissues from subjects without NPs. Levels of inflammatory mediators were measured by using real-time PCR, ELISA, and FlowCytomix analyses. Patients with NPs received oral corticosteroid therapy (30 mg of prednisone once daily for 7 days); clinical parameters of efficacy were associated with NP phenotypes.
Among patients with NPs, 76.5% had an eosinophilic phenotype, 46.0% had a neutrophilic phenotype, and 35.8% had a mixed phenotype (indicated by double staining). Overall, patients' symptoms improved after corticosteroid treatment; numbers of eosinophils and levels of their mediators (IL-4 and IL-5), but not numbers of neutrophils or levels of their mediators (IL-8 and interferon-inducible protein 10), were reduced (P< .05). After corticosteroid treatment, patients with the nonneutrophilic phenotype (neutrophil negative) had significantly greater reductions in bilateral polyp size scores, nasal congestion scores, total nasal symptom scores, and nasal resistance than patients with the neutrophilic phenotype (neutrophil positive, P< .05).
There are different phenotypes of NPs based on the type of immune cell infiltrate and cytokines produced (eosinophilic or neutrophilic). Patients with the neutrophilic phenotype have less response to treatment with corticosteroids based on symptom scores.
The Journal of allergy and clinical immunology 03/2012; 129(6):1522-8.e5. · 9.17 Impact Factor
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ABSTRACT: Recently, in vitro and in vivo models have identified that microRNAs (miRNAs), which are extensively expressed in the inner ear, play important roles in inner ear development and function. However, the function of miRNA in vertebrate tissue is not well understood.
The current study used an in vitro model of embryonic mouse inner ear in a stem/progenitor cell culture to demonstrate that: 1) miR-182 is expressed during differentiation of inner ear stem/progenitor cell into a hair cell-like fate, 2) ectopic miR-182 promotes inner ear stem/progenitor cell differentiation into a hair cell-like fate, and 3) the function of miR-182 may be associated with its putative target Tbx1, a transcription factors that have been implicated in inner ear development and hair cell fate.
Our findings suggest that miR-182 could regulate inner ear progenitor cell differentiation and that miRNAs are important regulators of hair cell differentiation, providing new targets for hair cell repair.
Hearing research 02/2012; 286(1-2):55-63. · 2.18 Impact Factor
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ABSTRACT: To evaluate the effects of NasoPore after packing of the middle ear in guinea pigs.
A randomized, prospective, controlled animal study.
University laboratory.
Forty-one guinea pigs were divided into 3 groups. In group 1(n=12), the middle ears of animals were unilaterally implanted with NasoPore, leaving the contralateral middle ears packed with absorbable gelatin sponge soaked in a solution containing kanamycin and furosemide as an ototoxicity-positive control; group 2 (n = 17) underwent the same experimental protocol as group 1, except the gelatin sponge was unsoaked; in group 3 (n = 12), NasoPore was inserted unilaterally and no packing material was placed into the contralateral ear. Auditory brainstem responses (ABRs) were performed preoperatively and 3 months after the procedure. The surface preparation and scanning electron microscopy (SEM) were assessed 3 months postoperatively, whereas pathology of middle ear was analyzed in 5 samples of group 2.
ABR thresholds of the contralateral ear significantly increased in group 1 and were slightly shifted in group 2 compared with the NasoPore-packed and nonpacked ears 3 months postoperatively. The NasoPore-packed middle ears were found to have less fibrosis and inflammation and less thickened bone and tympanic membranes than Gelfoam-packed ears. Surface preparations and SEM showed no ototoxicity in the inner ear of NasoPore-packed ears.
NasoPore appears to be effective for use in otosurgery. It caused less fibrosis in the middle ear than conventional packing agents and no ototoxicity to the inner ear.
Otolaryngology Head and Neck Surgery 03/2011; 145(1):131-6. · 1.72 Impact Factor
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ABSTRACT: Although microRNA (miRNA) is expressed extensively in the postnatal mouse inner ear, its expression in the sensory epithelium during embryogenesis has not been well characterized. We investigated miRNA expression at E13.5 and E16.5 by microarray analysis, quantitative real-time-PCR, and in-situ hybridization. MiRNA-182, miRNA-140, miRNA-200c, and others showed distinct temporal and spatial expression patterns. MiRNA-194, whose expression in zebrafish seems to play an important role in the differentiation of the intestinal epithelium, was also expressed in the spiral ganglia of the mouse inner ear, where it may play a similar role in neuronal differentiation. Our results indicate that miRNAs are widely expressed in the developing inner ear, with more species recruited as hair cells differentiate, suggesting an important developmental role.
Neuroreport 06/2010; 21(9):611-7. · 1.66 Impact Factor
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ABSTRACT: This study was designed to determine whether nasal saline irrigation improved the symptoms and signs of allergic rhinitis (AR) and whether nasal saline irrigation could be used as a complementary management of AR in children while less steroids were used.
26 children with AR were divided into three groups and were given nasal saline irrigation and/or topical steroid. Symptoms and signs of AR and mucociliary clearance (MCC) were evaluated, and concentration of soluble intercellular adhesion molecule (sICAM)-1 in nasal secretion was detected.
In AR children treated with nasal irrigation and tapered topical steroid at week 8 and week 12, a significant improvement in symptoms and signs was observed, and a significant decrease in the mean values of MCC and the mean concentrations of sICAM-1 in nasal secretions was also detected.
Nasal saline irrigation can be viewed as a good adjunctive treatment option for AR. It permitted the use of less topical steroids for controlling AR in children, which will contribute to fewer side effects and less economic burden.
ORL 01/2009; 71(1):50-5. · 0.91 Impact Factor
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ABSTRACT: To determine whether the chloride channel protein CLC-3 is upregulated in patients with allergic rhinitis (AR) and whether topical corticosteroid treatment decreases the expression of CLC-3.
Histologic study.
Academic research.
Eighteen patients with AR were included in the study.
Expression of CLC-3 was detected by immunohistochemistry and by reverse transcription-polymerase chain reaction before and at 4 weeks after treatment with the topical corticosteroid mometasone furoate.
Strong CLC-3 expression was detected in epithelium and in submucosal glands. An increased presence of CLC-3 was demonstrated in nasal mucosa compared with that in normal nasal tissue. A statistically significant difference in CLC-3 gene expression level was found in nasal tissues before vs at 4 weeks after treatment with mometasone.
CLC-3 may have a role in modulating the pathogenesis of AR. Decreased expression of CLC-3 may be the mechanism of action of corticosteroid treatment in controlling AR.
Archives of Otolaryngology - Head and Neck Surgery 04/2008; 134(3):301-5. · 1.63 Impact Factor
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ABSTRACT: We aimed to explore the transitional process of sinus cavity mucosa after functional endoscopic sinus surgery (FESS) and divide it into different stages.
The morphological characteristics of sinus cavity mucosa after FESS were continuously observed.
Over 90% of the nasal and sinus cavities got clean within 1-2 weeks, over 80% of the operative cavities experienced a reaction to mucosa removal or regenerative diseases within 3-10 weeks, such as mucosal edema, vesicles, granulation tissue, mini-polyps, fibrous hyperplasia and adhesion, which competed against the mucosa epithelialization process. After careful treatment, 90% of the nasal and sinus cavities finished epithelialization, and 60% of them completed epithelialization within 11-14 weeks after operation.
The recovery process of mucosa after FESS can be divided into 3 stages: the stage of clean operative cavity, the stage of mucosal transition and the stage of complete epithelialization. Proper management at the 3 stages is very essential to the whole curable effect of FESS.
ORL 02/2008; 70(2):118-23. · 0.91 Impact Factor
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ABSTRACT: To study the microanatomy by endoscope-assisted via retrolabyrinthine approach.
Nineteen cadaveric heads fixed with formalin were dissected in our study. The data that endoscope could be extent and the distance between the important point were measured. By simulated the retrolabyrinthine approach, endoscope was placed to observe the nerves and vessels, the distance between nerves and the central point of the anterior edge of sigmoid sinus were measured.
The distance from the inferior margin of petrous ridge to the upper bound of endolymphatic sac was (9.93+/-1.52)mm; and from superior margin of petrous ridge to the inferior margin common bony crus was (4.64+/-0.91)mm;and from the intersection of posterior semicircular canal to the anterior wall of sigmoid was (7.85+/-1.47)mm on the left, and (5.69+/-1.68)mm on the right. The distance from inferior margin common bony crus to the anterior wall of sigmoid was (13.9+/-1.71)mm on the left, and (11.31+/-2.03)mm on the right. The trochlear nerve and abducent nerve could be observed under endoscope. The relationship between the trigeminal nerve, acoustic nerve and the vessels could be identified clearly. The distance from the central point of the anterior edge of sigmoid to the roots of the trigeminal nerve, facial nerve, vestibulocochlear nerve and glossopharyngeal nerve were (29.88+/-2.77) mm, (32.04+/-2.04) mm, (29.17+/-1.65) mm, (35.49+/-1.53) mm respectively.
The visual field of the cerebellopontine angle appear wider by the endoscope assisted retrolabyrinthine approach. Nerves, vessel, internal acoustic pore, jugular foramen region can be clearly seen. Some minimal invasive surgery can be done by this approach.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 09/2007; 21(16):724-6.
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ABSTRACT: Allergic rhinitis (AR) is a Th2 predominant disease, and its pathogenic mechanism is still poorly understood. CD4(+)CD25(+) T cells account for approximately 5% to 10% peripheral CD4(+) T cells and has been shown to regulate the activation of effector T cells in the periphery. The activity of CD4(+)CD25(+) T cells is associated with the transcription factor Foxp3. The present study aimed to evaluate the possible role of CD4(+)CD25(+) T cells as well as Foxp3 in the pathogenesis of AR.
Nasal tissues and peripheral blood mononuclear cells (PBMCs) were obtained from 17 patients with AR and 11 control subjects. Foxp3 was detected in nasal tissues by immunohistochemistry and real-time reverse transcription-polymerase chain reaction (RT-PCR). CD4(+)CD25(+) T cells and Foxp3 were evaluated in PBMCs by using flow cytometry. Concentrations of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) were measured by enzyme-linked immunosorbent assay (ELISA) in cultured PBMCs in the presence or absence of stimulation with phorbol ester (PMA) and Ionomycin.
The numbers of Foxp3(+) cells was 129.5 +/- 35.6 and 44.2 +/- 20.5 cells/mm(2) in nasal mucosa of two groups (P < .05). There were less Foxp3(+) lymphocytes and decreased Foxp3 mRNA in AR compared with the control (P < .05). The frequencies of the CD4(+)CD25(+) population in PBMCs of two groups were 1.99 +/- 0.95% and 3.55 +/- 1.27% (P < .05). There was significant difference in the frequencies of the Foxp3(+)CD4(+) CD25(+) population (1.81 +/- 0.77 vs 3.37 +/- 1.04, P < .05) and mean fluorescence intensity (MFI) of Foxp3 (5.93 +/- 2.64 vs 11.72 +/- 4.29, P < .05) in PBMCs of two groups. After stimulation, the concentrations of IL-2 and IFN-gamma were 182.72 +/- 85.11 pg/mL and 348.94 +/- 151.88 pg/mL in PBMCs with AR, while those were 90.6 +/- 61.5 pg/mL and 155.64 +/- 68.33 pg/mL in controls (P < .05).
Our results indicate that CD4(+) CD25(+) regulatory T cells as well as Foxp3 may play a crucial role in immunological imbalance of AR. These findings suggest that increasing Foxp3 and CD4(+)CD25(+) T cells have the potential to be new therapeutic targets for the treatment of AR.
The Laryngoscope 06/2007; 117(5):876-80. · 1.75 Impact Factor
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ABSTRACT: A new way to calculate the complex propagation constant, γ=α+jβ, of a microstrip leaky wave antenna (MLWA) is presented. The calculated normalized complex propagation constant fits well with the comparison data. The properties of the leaky wave antenna in the first high mode are discussed using the simple cavity theory. It is explained, in principle, why the MLWA has a leaky region and critical frequency. Some simple rules about how to choose the width and permittivity of the MLWA are given.
Antennas and Propagation Society International Symposium, 2005 IEEE; 08/2005
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ABSTRACT: A new frequency-fixed dual-beam scanning microstrip leaky-wave antenna is reported. The beam-scanning ability at a fixed frequency of the antenna can be achieved with new feeding configurations. The main beam of the antenna may be tilted from 37° to 57° and from 125° to 143° in the H plane when the frequency is fixed.
Antennas and Propagation Society International Symposium, 2004. IEEE; 07/2004
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ABSTRACT: A novel antenna array for LMDS applications is presented. It is a coplanar cellular-distributing microstrip antenna array and is made up of six-element subarrays. Each rectangular microstrip patch of the subarray is fed via a corporate-feed network, and each feed network of the subarray is accomplished by a coaxial probe through the ground plane. The new antenna may be an alternative for LMDS applications. Simulated results are given and compared with those arrays operating at the same band.
Antennas and Propagation Society International Symposium, 2004. IEEE; 07/2004
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ABSTRACT: A novel dual-frequency microstrip patch antenna for WLAN applications is presented and demonstrated. By embedding two pairs of arc shaped slots with a narrow slot protruding at each side, dual-band operation, with its operating mode centered at about frequencies 2.4 GHz and 5.2 GHz, has been obtained. The proposed antenna has good gain and its impedance bandwidths defined by VSWR=2 can meet the requirements of dual ISM bands.
Antennas and Propagation Society International Symposium, 2004. IEEE; 07/2004
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ABSTRACT: Great attention has been paid to conveniently calculating the
electromagnetic (EM) field due to a vertical magnetic dipole (VMD)
buried in stratified media. It is quite difficult because this topic
involves the computation of Sommerfeld (1949) type integrals (SI). In
this paper closed-form expressions for the far field of a VMD embedded
below the sea surface are obtained easily by using a new technique to
evaluate the SI with the aid of complex image theory. The present
approach can be also used to get the far field formulas for other
similar cases
IEEE Transactions on Antennas and Propagation 07/2001; · 2.15 Impact Factor
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ABSTRACT: The Kuhn algorithm is introduced firstly and the numerical methods for resolving the complex transcendental equations in the electromagnetic theory are researched. The numerical examples are given to confirm the validity and efficiency of the numerical methods.
The Kuhn algorithm is introduced firstly and the numerical methods for resolving the complex transcendental equations in the electromagnetic theory are researched. The numerical examples are given to confirm the validity and efficiency of the numerical methods.
International Journal of Infrared and Millimeter Waves 04/1998; 19(5):785-790. · 0.58 Impact Factor
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ABSTRACT: The radiation of a loop antenna embedded in a borehole with
complicated geometry is analyzed by perturbation method and an efficient
fast multiple-integration technique. The perturbation method is not used
directly because there is a drill-stem whose conductivity and magnetic
permeability are very large in the borehole. The prolate ellipsoid
equivalence of the metal drill-rod is made, then the drill-rod may be
removed and the perturbation method is valid. Some numerical results
indicate that the approach is efficient and stable at low frequencies
IEEE Transactions on Geoscience and Remote Sensing 02/1996; · 2.89 Impact Factor
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ABSTRACT: Chronic rhinosinusitis (CRS) may cover different disease entities, and the pathogenic mechanism remains unclear.
The aim of this study was to evaluate the expression of chloride channel protein CLC-2 and CLC-3 in CRS without nasal polyps (CRSsNP) and evaluate the roles of interleukin (IL)-4 and transforming growth factor (TGF) beta in the up-regulation of CLC-2 and CLC-3. We detected expression of CLC-2 and CLC-3 in 17 patients with CRSsNP by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), and we examined the concentration of TGF-beta, IL-4, IL-5, and interferon (IFN) gamma in ethmoid sinus mucosa by enzyme-linked immunosorbent assay (ELISA).
We found that CLC-2 and CLC-3 is up-regulated in CRSnNP and located in submucosal glands and epithelium of the ethmoid sinus. CLC-2 and CLC-3 mRNA correlated with IL-4 in CRSsNP (r = 0.57 and 0.65; p < 0.05). CLC-2 and CLC-3 mRNA correlated negatively with mucosal TGF-beta in CRSsNP (r = -0.49 and -0.54; p < 0.05).
We concluded that CLC-2 and CLC-3 is up-regulated in ethmoid mucosa and may affect the development of CRSsNP. TGF-beta and IL-4 may modulate the expression of CLC-2 and CLC-3 in CRSsNP.
American Journal of Rhinology 21(4):389-94. · 1.36 Impact Factor
