Hong Wang

Publications (97)110.24 Total impact

• Article: Effect of pcDNA3.1- vascular endothelial growth factor 165 recombined vector on vascular buds in rabbit vertebral cartilage endplate.

  Hong-Guang Xu, Xiao-Hu Chen, Guo-Zheng Ding, Hong Wang, Ling-Ting Wang, Xue-Wu Chen
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  ABSTRACT: This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor (VEGF)165 vector on vertebral cartilage endplate vascular buds and intervertebral discs. Rabbits were randomly assigned to the control and experimental groups with 10 in each. In the experimental group, we anesthetized the rabbits and exposed the front vertebral body. Using the mark of the longitudinal ossature of the front vertebral body of the lumbar vertebrae, we advanced a needle at the central point of the front fourth and fifth lumbar intervertebral discs and injected 20 µl pcDNA3.1-VEGF165. Similarly, in the control group, we injected 20 µl pcDNA3.1. At 4 and 8 weeks post-injection, we examined the changes of the vertebral cartilage endplate using X-ray radiograph, histology, and scanning electron microscopy. The vertebral cartilage endplate calcification and degeneration in the experimental group were less than those in the control group at 8 weeks post-operation. The average number and diameter of vascular buds obviously increased in the experimental group at 4 and 8 weeks post-operation. The number of vascular buds and the diameter in the region of the inner annulus increased when compared to those in the area near the nucleus pulposus. The pcDNA3.1-VEGF165 plasmid can increase the average number and diameter of vascular buds and decelerate intervertebral disc degeneration.
  Chinese medical journal 11/2012; 125(22):4055-60. · 0.86 Impact Factor
• Article: Real-time and in-situ cell imaging of thiol compounds in living cells using maleimide BODIPY labeling.

  Xiao-Feng Guo, Jian-Bo Chen, Hong Wang, Hua-Shan Zhang, Wei-Hua Huang, Jian Guo
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  ABSTRACT: In this paper, a simple analytical method for the visualization of thiol compounds including glutathione (GSH), N-acetylcysteine (NAC), cysteine (Cys) and coenzyme A (CoA) in living cells is developed based on 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene (TMPAB-o-M) labeling. By using of this fluorogenic reagent, in-situ imaging of thiol compounds in cells could be achieved in only 90s, which is much faster than that mentioned in other reports. The fluorescence of derivative products in living cells could be stable for atleast 15min under irradiation, and can be quantified by HPLC easily in only 6min.
  Talanta 09/2012; 99:1046-50. · 3.79 Impact Factor
• Article: A model for the estimation of fractional vegetation cover based on the relationship between vegetation and soil moisture

  Xiaobing Li, Hong Wang, Huiling Long, Dandan Wei, Yun Bao
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  ABSTRACT: Based on surface temperature and the normalized difference vegetation index (NDVI), we calculated the temperature vegetation dryness index (TVDI). Using the relationship between TVDI and NDVI, we established a vegetation–soil moisture response model that captures the sensitivity of NDVI's response to changes in TVDI using a linear unmixing approach, and validated the model using Landsat Thematic Mapper (TM) images acquired in 1997, 2004 and 2006 and a Landsat Enhanced Thematic Mapper Plus (ETM+) image acquired in 2000. We determined the correlations between TVDI and field-measured soil moisture in 2006. TVDI was correlated significantly with soil moisture at depths of 0 to 10 cm and 10 to 20 cm, so TVDI can be used as an index that captures changes in soil moisture at these depths. By using fractional vegetation cover (FVC) data measured in the field to validate the estimated values, we estimated mean absolute errors of 0.043 and 0.137 for shrub and grassland vegetation coverage, respectively, demonstrating acceptable estimation accuracy. Based on these results, it is possible to estimate a region's FVC using the linear unmixing model. The results show bare land coverage values distributed similarly to TVDI values. In mountain areas, grassland coverage mostly ranged from 0.4 to 0.6. Shrub coverage mostly ranged from 0.4 to 0.6. Forest coverage was zero in most parts of the study area.
  International Journal of Remote Sensing 06/2012; 33(11):3580-3595. · 1.12 Impact Factor
• Article: Numerical Simulation on Coalescence Between a Pair of Drops on Homogeneous Horizontal Surface with Volume-of-Fluid Method

  Hong Wang, Xun Zhu, Qiang Liao, P. C. Sui
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  ABSTRACT: Two-dimensional transient simulations employing the volume-of-fluid (VOF) method are used to investigate the behavior of coalescence between a pair of equal size water droplets on a homogeneous plate surface. The morphological change of the liquid drops and variation of the meniscus liquid bridge during the coalescence are obtained. The simulations show that a meniscus liquid bridge is formed once two drops merge. As time progresses, the liquid bridge radius increases to reach a maximum value accompanying with the internal liquid flow in drops, and then decreases to tend to a periodic damped oscillation. Furthermore, the simulations are compared with experimental data under same conditions. The good agreement verifies that the VOF method can be used to predict the behaviors of coalescence between drops on homogeneous horizontal surface, especially for the early stage of the coalescence. KeywordsVolume of fluid method-Water drops coalescence-Homogeneous surface-Liquid bridge
  Journal of Superconductivity and Novel Magnetism 05/2012; 23(6):1137-1140. · 0.65 Impact Factor
• Article: Microwave dielectric properties of low-firing BiNbO4 ceramics with V2O5 substitution

  Di Zhou, Hong Wang, Xi Yao, Yun Liu
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  ABSTRACT: The effect of V2O5 substitution on the sintering behavior and the microwave dielectric properties of BiNbO4 ceramics were studied. The sintering temperatures of Bi(V x Nb1−x )O4 ceramics decrease from 990 to 810°C with x value increasing from 0.002 to 0.064. The size of grains increased with the sintering temperature increasing and decreased with the substitution amount increasing. The dielectric properties are affected by the microstructures very much. The quality factor Q value is from 2500 to 4000 at about frequency = 5GHz and reach to the maximum when x = 0.032. With the different x value, the Q f values change between 15000 to 20000GHz; the τ f values changes between 0 and +20ppm/°C between temperature range 25∼85°C and decreased with the increasing of x value.
  Journal of Electroceramics 04/2012; 21(1):469-472. · 1.19 Impact Factor
• Article: Experimental Studies of Liquid Droplet Coalescence on the Gradient Surface

  Hong Wang, Qiang Liao, Xun Zhu, Jun Li, Xin Tian
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  ABSTRACT: Visual experiments were conducted to study the behaviors of liquid droplet coalescence on the surface with gradient surface energy. The microscopic contour of the gradient energy surface, which was fabricated on the base of a silicon chip by diffusion-controlled silanization of alkyltrichlorosilanes, was characterized by atom force microscopy. The effects of droplets on the three-phase contact line and the contact angle were obtained. It was observed that the process of droplet coalescence accelerates the droplet speed on the gradient surface. On the high-energy surface, the triple-phase contact line of the coalescing droplet is prone to pinning. The interfacial energy released from coalescence is the main source for promoting the motion. Furthermore, the nonequilibrium capillary pressure gradient inside coalescence droplets is considered to aid in accelerating the coalescence. KeywordsCoalescence-Contact line-Contact angle-Gradient surface
  Journal of Superconductivity and Novel Magnetism 04/2012; 23(6):1165-1168. · 0.65 Impact Factor
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  Article: Molecular dynamics simulation of injection of polyethylene fluid in a variable cross-section nano-channel

  Jie Feng, Qiang Liao, Xun Zhu, Rui Wu, Hong Wang, YuDong Ding, Kisaragi Yashiro
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  ABSTRACT: Numerical simulation of injection of polyethylene fluid in a variable cross-section nano-channel was carried out using the molecular dynamics method. The effects of the nano-channel cross-section and the external force on the rheological behavior and structural properties of the polyethylene fluid were investigated. It was found that an absorbed layer appeared near the wall and the thickness of the absorbed layer increased with increasing cone angle of the nano-channel. The injection distance of the polyethylene fluid decreased with increasing cone angle and decreasing external force. In the nano-channel with cone angle 45°, polyethylene particles uniformly filled the whole channel and were stretched along the flow direction. Uniaxial stretching of particles was enhanced when the external force was strengthened, which facilitates injection of the polyethylene fluid into the nano-channel. Keywordsmolecular dynamics simulation–polyethylene–injection process–nano-channel
  Chinese Science Bulletin 04/2012; 56(17):1848-1856. · 1.32 Impact Factor
• Article: [Radiofrequency catheter ablation for ventricular premature beats of left coronary cusp under the guidance of 3-dimensional mapping system].

  Hong Wang, Lang Hong, Yuan-feng Zhou, Heng-li Lai, Zai-hua Chen, Yun Qiu
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  ABSTRACT: To explore the efficacy and safety of radiofrequency catheter ablation (RCA) for ventricular premature beats originating from left coronary sinus under the guidance of 3-dimensional mapping system (CARTO). A total of 15 patients with premature ventricular contractions (PVCs) originating from left coronary sinus underwent CARTO-guided RCA. Anatomical structures were constructed and three-dimension (3D) electrical activation sequence was plotted for left ventricle and aortic sinus. The distance of earliest activation point of PVCs and origin of left coronary artery were surveyed after left coronary arteriography. The electrocardiogram (ECG) results showed that R-wave was upward in leads II, III and avF, QRS waves in lead I was mainly of rS, rs and rsr types, QS type in lead avL, RS, Rs and rS type in lead V(1), RS type in lead V(3) and absence of S wave in lead V(5)/V(6). Intraoperative mapping detected the earliest activation point on the posterio-inferior origin of left coronary artery (LMCA) ostium (n = 7), on the anterio-inferior of LMCA ostium (n = 3) and on the inferior of LMCA ostium (n = 5). The earliest activation point (local activation time) was shorter 86 - 120 ms than surface electrocardiogram QRS wave, discharge melting on the earliest activation point and nearby succeeded. PVCs disappeared, PVCs failed to be induced under similar preoperative conditions (aleudrin intravenous) and no complication occurred intraoperatively and postoperatively. The CARTO-guided RCA is a safe and effective in the treatment of PVCs originating from left coronary sinus.
  Zhonghua yi xue za zhi 04/2012; 92(14):977-9.
• Article: Roles for peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ coactivators 1α and 1β in regulating response of white and brown adipocytes to hypoxia.

  Elizabeth Pino, Hong Wang, Meghan E McDonald, Li Qiang, Stephen R Farmer
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  ABSTRACT: Obese white adipose tissue is hypoxic but is incapable of inducing compensatory angiogenesis. Brown adipose tissue is highly vascularized, facilitating delivery of nutrients to brown adipocytes for heat production. In this study, we investigated the mechanisms by which white and brown adipocytes respond to hypoxia. Brown adipocytes produced lower amounts of hypoxia-inducible factor 1α (HIF-1α) than white adipocytes in response to low O(2) but induced higher levels of hypoxia-associated genes. The response of white adipocytes to hypoxia required HIF-1α, but its presence alone was incapable of inducing target gene expression under normoxic conditions. In addition to the HIF-1α targets, hypoxia also induced many inflammatory genes. Exposure of white adipocytes to a peroxisome proliferator-activated receptor γ (PPARγ) ligand (troglitazone) attenuated induction of these genes but enhanced expression of the HIF-1α targets. Knockdown of PPARγ in mature white adipocytes prevented the usual robust induction of HIF-1α targets in response to hypoxia. Similarly, knockdown of PPARγ coactivator (PGC) 1β in PGC-1α-deficient brown adipocytes eliminated their response to hypoxia. These data demonstrate that the response of white adipocytes requires HIF-1α but also depends on PPARγ in white cells and the PPARγ cofactors PGC-1α and PGC-1β in brown cells.
  Journal of Biological Chemistry 04/2012; 287(22):18351-8. · 4.77 Impact Factor
• Article: Discovery and Optimization of a Novel Spiropyrrolidine Inhibitor of β-Secretase (BACE1) through Fragment-Based Drug Design.

  Ivan V Efremov, Felix F Vajdos, Kris A Borzilleri, Steven Capetta, Hou Chen, Peter H Dorff, Jason K Dutra, Steven W Goldstein, Mahmoud Mansour, Alexander McColl, Stephen Noell, Christine E Oborski, Thomas N O'Connell, Theresa J O'Sullivan, Jayvardhan Pandit, Hong Wang, Binqing Wei, Jane M Withka
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  ABSTRACT: The aspartyl protease β-secretase, or BACE, has been demonstrated to be a key factor in the proteolytic formation of Aβ-peptide, a major component of plaques in the brains of Alzheimer's disease (AD) patients, and inhibition of this enzyme has emerged as a major strategy for pharmacologic intervention in AD. An X-ray-based fragment screen of Pfizer's proprietary fragment collection has resulted in the identification of a novel BACE binder featuring spiropyrrolidine framework. Although exhibiting only weak inhibitory activity against the BACE enzyme, the small compound was verified by biophysical and NMR-based methods as a bona fide BACE inhibitor. Subsequent optimization of the lead compound, relying heavily on structure-based drug design and computational prediction of physiochemical properties, resulted in a nearly 1000-fold improvement in potency while maintaining ligand efficiency and properties predictive of good permeability and low P-gp liability.
  Journal of Medicinal Chemistry 04/2012; · 4.80 Impact Factor
• Article: [The influence of statin therapy on circulating microRNA-92a expression in patients with coronary heart disease].

  Hong Wang, Hong-mei Lu, Wen-hui Yang, Cheng Luo, Shan-he Lu, Ying Zhou, Ying-zhong Lin
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  ABSTRACT: To investigate the modulatory function of statin therapy on circulating microRNA-92a (miR-92a) in patients with coronary heart disease (CHD), and to evaluate the possibility of miR-92a as a new target of treatment for endothelial dysfunction. A case control study was conducted. Prevalence of abnormal blood fat content, statin treatment rate, and attainment rate of low density lipoprotein-cholesterol (LDL-C) lowered to expected level in 236 patients with CHD were analyzed. Relationship between statin therapy in patients with type 2 diabetes mellitus (DM), and level of LDL-C and circulating miR-92a expression was analyzed by multivariate general linear factorial analysis. The incidence of acute coronary syndrome (ACS) was compared in patients with CHD receiving statin therapy in all groups. Prevalence of abnormal LDL-C was 95.7% (112/117) in CHD patients of non-statin therapy group, and 47.5% (112/236) of patients with CHD who should receive statin therapy but did not. Attainment rate of lowering of LDL-C to expected level in statin therapy group was 27.7% (33/119). LDL-C level (mmol/L) was significantly lower in statin therapy group than that in non-statin therapy group (2.457 ± 0.802 vs. 3.218 ± 1.130, Z = -9.760, P = 0.001), and incidence of ACS was significantly lower in statin therapy group than that in non-statin therapy group [33.6% vs. 71.8%, χ(2) = 34.491, P = 0.001]. There was no significant difference in incidence of ACS between patients with or without attaining the expected low value of LDL-C in statin therapy group [33.3% vs. 33.7%,χ(2) = 0.002, P = 0.968]. Circulating miR-92a expression was significantly higher in patients with stable angina pectioris (SAP) complicated with DM than those without DM (0.492 vs. -0.121, Z = -3.038, P = 0.002). It was found that statin therapy could down regulate miR-92a expression in patients with SAP complicated with DM as compared with that with non-statin therapy (0.419 vs. 0.687, Z = 1.289, P = 0.072). There was no significant difference in circulating miR-92a expression between statin therapy and non-statin therapy in patients with SAP without co-existing DM (-0.032 vs. -0.198, Z = -0.614, P = 0.539). Multivariate general linear factorial analysis showed that statin therapy was the major influential factor on LDL-C level in patients with CHD (F = 22.863, P = 0.001), and complicating DM was the major influential factor on circulating miR-92a expression in patients with SAP (F = 9.641, P = 0.003). Regulation of circulating miR-92a expression may be considered as a new clinical target for statin treating endothelial dysfunction in patients with CHD.
  Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 04/2012; 24(4):215-8.
• Article: Developing mutually encapsulating materials for versatile syntheses of multilayer metal-silica-polymer hybrid nanostructures.

  Liangfang Zhu, Hong Wang, Xiaoshuang Shen, Liyong Chen, Yawen Wang, Hongyu Chen
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  ABSTRACT: New methods to self-assemble polystyrene-block-poly(acrylic acid) (PSPAA) on silica via electrostatic interaction and to deposit silica on PSPAA shells are developed. The mutual encapsulation of silica and PSPAA allows versatile syntheses of well-controlled nanohybrids.
  Small 03/2012; 8(12):1857-62. · 8.35 Impact Factor
• Article: Cohort Profile: Longitudinal Investigations into Supportive and Ancillary health services.

  Katrina C Duncan, Kate Salters, Jamie I Forrest, Alexis K Palmer, Hong Wang, Nadia O'Brien, Surita Parashar, Angela M Cescon, Hasina Samji, Julio Sg Montaner, Robert S Hogg
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  ABSTRACT: The Longitudinal Investigations into Supportive and Ancillary health services (LISA) study is a cohort of people living with HIV/AIDS who have ever accessed anti-retroviral therapy (ART) in British Columbia, Canada. The LISA study was developed to better understand the outcomes of people living with HIV with respect to supportive services use, socio-demographic factors and quality of life. Between July 2007 and January 2010, 1000 participants completed an interviewer-administered questionnaire that included questions concerning medical history, substance use, social and medical support services, food and housing security and other social determinants of health characteristics. Of the 1000 participants, 917 were successfully linked to longitudinal clinical data through the provincial Drug Treatment Program. Within the LISA cohort, 27% of the participants are female, the median age is 39 years and 32% identify as Aboriginal. Knowledge translation activities for LISA include the creation of plain language summaries, internet resources and arts-based engagement activities such as Photovoice.
  International Journal of Epidemiology 03/2012; · 6.41 Impact Factor
• Article: Protection against pneumococcal infection elicited by immunization with glutamyl tRNA synthetase, polyamine transport protein D and sortase A.

  Xun Min, Xuemei Zhang, Hong Wang, Yi Gong, Meijuan Li, Wenchun Xu, Yibing Yin, Ju Cao
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  ABSTRACT: Protein-based vaccines are considered to be the next-generation of pneumococcal vaccines. Here we evaluated the protection elicited by immunization with recombinant glutamyl tRNA synthetase (Gts), polyamine transport protein D (PotD) and sortase A (SrtA) antigens in preclinical mouse models. In mucosal immunization studies, intranasal immunization with either Gts, PotD or SrtA could significantly reduce pneumococcal nasopharyngeal and lung colonization and significantly increase mice survival times following invasive pneumococcal challenge, and combinations of these antigens could enhance this protection. In systemic immunization studies, intraperitoneal immunization with multiple protein antigens also provided better protection against pneumococcal sepsis caused by different pneumococcal strains. Finally, passive immunization studies showed an additive effect by using multiple anti-sera when compared to single anti-sera. Therefore, a multicomponent protein-based pneumococcal vaccine composed of Gts, PotD or SrtA could confer protection against pneumococcal colonization as well as invasive infections in terms of efficacy of protection and serotype coverage.
  Vaccine 03/2012; 30(24):3624-33. · 3.77 Impact Factor
• Article: Mechanisms of angiotensin II-enhanced connecting tubule glomerular feedback.

  Yilin Ren, Martin A D'Ambrosio, Hong Wang, Edward L Peterson, Jeffrey L Garvin, Oscar A Carretero
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  ABSTRACT: Increasing Na delivery to the connecting tubule (CNT) causes afferent arteriole (Af-Art) dilation, a process we call CNT glomerular feedback (CTGF). Angiotensin II (ANG II) in the CNT lumen enhances CTGF via PKC. We hypothesized that luminal ANG II stimulates CTGF via activation of protein kinase C (PKC), NADPH oxidase 2 (NOX2), and enhanced production of superoxide (O(2)(-)). Rabbit Af-Arts and adherent CNTs were microdissected and microperfused in vitro. Dilation of the Af-Art was induced by increasing luminal CNT NaCl from 0 to 5, 10, 30, 45, and 80 mM, and the concentration of NaCl that elicited a half-maximal response (EC(50)) was calculated. Compared with vehicle, adding ANG II (10(-9) M) to the CNT lumen reduced EC(50) from 37 ± 3 to 14 ± 1 mM (P < 0.001), indicating ANG II potentiates CTGF. In the presence of ANG II, the O(2)(-) scavenger tempol (10(-4) M) increased EC(50) from 20 ± 4 to 41 ± 3 mM (P < 0.01), the NOX inhibitor apocynin (10(-5) M) increased EC(50) from 17 ± 2 to 39 ± 4 mM (P < 0.01), and the specific NOX2 inhibitor gp91ds-tat (10(-5) M) increased EC(50) from 19 ± 2 to 34 ± 2 mM (P < 0.01). However, tempol, apocynin, and gp91ds-tat had no effect on CTGF in the absence of ANG II. Compared with vehicle, the PKC activator PMA (2 × 10(-7) M) decreased EC(50) from 35 ± 2 to 14 ± 1 (P < 0.001). In the presence of PMA, tempol increased EC(50) from 14 ± 2 to 35 ± 2 mM (P < 0.01). We conclude the PKC/NOX2/O(2)(-) pathway mediates the enhancement of CTGF by luminal ANG II but it does not participate in CTGF in the absence of ANG II.
  AJP Renal Physiology 03/2012; 303(2):F259-65. · 4.42 Impact Factor
• Article: Usefulness of intravenous adenosine in idiopathic pulmonary arterial hypertension as a screening agent for identifying long-term responders to calcium channel blockers.

  Xiang-Rong Zuo, Rui Zhang, Xin Jiang, Xin-Li Li, Feng Zong, Wei-Ping Xie, Hong Wang, Zhi-Cheng Jing
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  ABSTRACT: Although intravenous adenosine is recommended for acute vasodilator testing in patients with pulmonary hypertension, long-term outcomes in acute responders treated with calcium channel blockers (CCBs) who are identified by adenosine remain unknown. In this study, the value of adenosine for identifying long-term responders to CCBs was investigated in patients with idiopathic pulmonary arterial hypertension (IPAH). All acute responders were subsequently treated with high-dose CCB monotherapy, and 6-minute walk distances, hemodynamic data, and World Health Organization functional classifications were followed. Nine of 104 patients exhibited an acute response with intravenous adenosine (8.7%, 95% confidence interval 3.2 to 14.2). After 12 months of follow-up, all acute responders were still alive; however, only 6 patients showed sustained hemodynamic improvement (5.8%, 95% confidence interval 2 to 13). Three patients had failed CCB monotherapy and bosentan was added to their treatment. Mean tolerated dose of intravenous adenosine was 142 ± 49 μg/kg/min. No life-threatening adverse events were observed and only 2 patients of the nonresponders exhibited a 20% decrease in mean systemic arterial pressure. In nonresponders, 1- and 3-year survival rates were 89% and 75%, respectively. In conclusion, acute vasodilator testing with intravenous adenosine was safe and able to screen responders to CCB therapy in patients with IPAH. Long-term CCB responders accounted for about 5.8% of patients with IPAH.
  The American journal of cardiology 03/2012; 109(12):1801-6. · 3.58 Impact Factor
• Article: Role of a novel pyridostigmine bromide-phospholipid nanocomplex in improving oral bioavailability.

  Qun-you Tan, Ni-ni Hu, Guo-dong Liu, Hua-feng Yin, Li Zhang, Hong Wang, Lu-yang Lu, Jing-qing Zhang
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  ABSTRACT: A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X(1), X(2), and X(3) were 8, 40°C, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and -25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (T(max)) 2 h vs 2 h, the maximum concentration (C(max)) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC(0-∞)) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.
  Archives of Pharmacal Research 03/2012; 35(3):499-508. · 1.59 Impact Factor
• Article: Iptakalim rescues human pulmonary artery endothelial cells from hypoxia-induced nitric oxide system dysfunction.

  Feng Zong, Xiang-Rong Zuo, Qiang Wang, Shi-Jiang Zhang, Wei-Ping Xie, Hong Wang
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  ABSTRACT: The aim of this study was to assess whether hypoxia inhibits endothelial nitric oxide synthase (eNOS) activity and nitric oxide (NO) production, and whether iptakalim may rescue human pulmonary artery endothelial cells (HPAECs) from hypoxia-induced NO system dysfunction. HPAECs were cultured under hypoxic conditions in the absence or presence of 0.1, 10 and 1,000 μM iptakalim or the combination of 10 μM iptakalim and 1, 10 and 100 μM glibenclamide for 24 h, and the eNOS activity and NO levels were measured in the conditioned medium from the HPAEC cultures. The eNOS activity and NO levels were reduced significantly in the conditioned medium from HPAEC cultures under hypoxic conditions. Pre-treatment with 10 μM iptakalim normalized the reduction of the eNOS activity and NO levels caused by hypoxia in the conditioned medium from HPAEC cultures. Iptakalim raised the eNOS activity and NO levels under hypoxic conditions, but was blocked by the K(ATP) channel blocker, glibenclamide. Our results indicate that hypoxia impairs NO system function, whereas the ATP-sensitive K(+) channel opener, iptakalim, may rescue HPAECs from hypoxia-induced NO system dysfunction.
  Experimental and therapeutic medicine 03/2012; 3(3):535-539.
• Article: [Effects of vascularendothelial growth factor vector on vascular buds of vertebral cartilaginous endplate in rabbits].

  Hong-Guang Xu, Guo-Zheng Ding, Xiao-Hu Chen, Hong Wang, Ling-Ting Wang, Xue-Wu Chen
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  ABSTRACT: To directly inject recombinant pcDNA3.1-VEGF165 plasmid into degeneration interverbral disc and explore its effects on vascular buds of vertebral cartilage endplate and intervertebral disc in rabbits. Rabbits were randomly assigned into the experimental and control groups (n = 10 each). For the experimental group, the animals were anesthetized and the front vertebral body exposed. With the longitudinal ossature of front vertebral body of lumbar vertebrae as a mark, a needle was inserted at the central point of the front fourth and fifth lumbar intervertebral disc and 20 µl pcDNA3.1-VEGF165 injected. For the control group, 20 µl pcDNA3.1 was injected. At Weeks 4 and 8 post-injection, the changes of vertebral cartilage endplate were monitored by radiograph, histology and scanning electron microscopy. The vertebral cartilage endplate calcification and degeneration in the experimental group were less pronounced than that in the control group at Week 8 post-operation. The average number and diameter of vascular buds obviously increased in the experimental group at Weeks 4 and 8 post-operation. The number and diameter of vascular buds in the region of inner annulus increased compared with those in the area near nucleus pulposus. The pcDNA3.1-VEGF165 plasmid may promote the vascular buds of vertebral cartilage endplate by increasing their average number and diameter and arresting the intervertebral disc degeneration.
  Zhonghua yi xue za zhi 02/2012; 92(7):491-5.
• Article: ROR2 gene is associated with risk of non-syndromic cleft palate in an Asian population.

  Hong Wang, Jacqueline B Hetmanski, Ingo Ruczinski, Kung Yee Liang, M Daniele Fallin, Richard J Redett, Gerald V Raymond, Yah-Huei Wu Chou, Philip Kuo-Ting Chen, Vincent Yeow, Samuel S Chong, Felicia Sh Cheah, Ethylin Wang Jabs, Alan F Scott, Terri H Beaty
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  ABSTRACT: The receptor tyrosine kinase-like orphan receptor 2 (ROR2) gene has been recently shown to play important roles in palatal development in animal models and resides in the chromosomal region linked to non syndromic cleft lip with or without cleft palate in humans. The aim of this study was to investigate the possible association between ROR2 gene and non-syndromic oral clefts. Here we tested 38 eligible single-nucleotide polymorphisms (SNPs) in ROR2 gene in 297 non-syndromic cleft lip with or without cleft palate and in 82 non-syndromic cleft palate case parent trios recruited from Asia and Maryland. Family Based Association Test was used to test for deviation from Mendelian inheritance. Plink software was used to test potential parent of origin effect. Possible maternally mediated in utero effects were assessed using the TRIad Multi-Marker approach under an assumption of mating symmetry in the population. Significant evidence of linkage and association was shown for 3 SNPs (rs7858435, rs10820914 and rs3905385) among 57 Asian non-syndromic cleft palate trios in Family Based Association Tests. P values for these 3 SNPs equaled to 0.000068, 0.000115 and 0.000464 respectively which were all less than the significance level (0.05/38 = 0.0013) adjusted by strict Bonferroni correction. Relevant odds ratios for the risk allele were 3.42 (1.80 - 6.50), 3.45 (1.75 - 6.67) and 2.94 (1.56 - 5.56), respectively. Statistical evidence of linkage and association was not shown for study groups other than non-syndromic cleft palate. Neither evidence for parent-of-origin nor maternal genotypic effect was shown for any of the ROR2 markers in our analysis for all study groups. Our results provided evidence of linkage and association between the ROR2 gene and a gene controlling risk to non-syndromic cleft palate.
  Chinese medical journal 02/2012; 125(3):476-80. · 0.86 Impact Factor