[show abstract][hide abstract] ABSTRACT: Background and aims: Large artery stiffness and endothelial dysfunction are the predominant characteristic of isolated systolic hypertension. Recently studies have revealed MMP1, 3, 9 and TIMP3 Genes polymorphism were associated with arterial stiffness, but the relationship with isolated systolic hypertension were not further studied. This study was to investigate the associations of MMP1,3,9 and TIMP3 Genes polymorphism with isolated systolic hypertension. Methods: We identified the genotype of the genes in 503 patients with isolated systolic hypertension, 481 essential hypertension patients with elevated diastolic blood pressure and 244 age-matched normotensive controls for 5 SNPs and detected the brachial-ankle pulse wave velocity, flow-mediated dilatation, endothelin-1 and nitric oxide among the participants. Results: Multinomial logistic analyses showed that the 5A allele of rs3025058(5A/6A) in MMP3 and the T allele of rs3918242(C-1562T) in MMP9 were significantly associated with isolated systolic hypertension after adjusted by age, triglyceride, low-density lipoprotein (P<0.001, Pcorr<0.003; P=0.009, Pcorr=0.027). The 5A/G/C and 6A/A/T haplotypes were significantly associated with isolated systolic hypertension (Permutation p=0.0258; Permutation p=0.000002). In addition, the brachial-ankle pulse wave velocity of different genotypes for the 5A/6A and C-1562T polymorphisms was significantly highest in 5A or T homozygotes (P<0.01), however, the flow-mediated dilatation and nitric oxide were markedly lowest in 5A or T homozygotes (P<0.01). Conclusion: MMP3 and MMP9 genes variant seem to contribute to the development of isolated systolic hypertension by affecting arterial stiffness and endothelial function.
International journal of medical sciences 01/2013; 10(7):840-7. · 2.07 Impact Factor
[show abstract][hide abstract] ABSTRACT: To assess the diagnostic value of 8 equations using different variables for determining the estimated glomerular filtration rate (eGFR) in patients with cardiovascular diseases.
GFR was estimated in 208 patients with cardiovascular diseases by (99m)Tc-DTPA dynamic renal imaging, and the eGFR was derived from 8 equations using different variables.
In patients with chronic kidney disease (CKD) stages 1-3, the eGFR calculated suing serum creatinine (SCr)-based equation was better correlated to GFR estimated by (99m)Tc-DTPA renal imaging than that derived from cystatin C (Cys C)-based equations, whereas in patients with CKD stages 4 and 5, the estimates by the latter equation showed a better correlation to GFR. Compared with (99m)Tc-DTPA renal imaging, MDRD-based equation and simple MDRD equation resulted in a higher eGFR in patients with CKD stages 4 and 5, the Rule equation had a lower eGFR in CKD stages 1 and 2, the Macisaac equation yielded a higher eGFR in CKD stages 2-5, and the Tan equation showed a higher eGFR in CKD stages 2 and 3. In patients with mild renal dysfunction, the Scr-based equation had a higher AUC(ROC) than Cys C-based equation, which was reversed in patients with severe renal dysfunction; the AUC(ROC) of the two equations were comparable in patients with moderate renal dysfunction. Compared with (99m)Tc-DTPA renal imaging, the modified MDRD equation and Arnal-Dade equation showed no significant difference in the eGFR in patients with CKD stages 1-5.
Modified MDRD equation (or simple MDRD equation) and Arnal-Dade equation are superior to other calculation methods for estimating the GFR in Chinese patients with cardiovascular disease.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 07/2011; 31(7):1220-3.
[show abstract][hide abstract] ABSTRACT: To investigate effects of the furosemide, antisterone and hydrochlorothiazide on expression of kidney aquaporin-2 (AQP(2)) gene and urine aquaporin-2 excretion in rats.
Forty SD rats were randomized into 4 groups, namely the control group, furosemide group, antisterone group and hydrochlorothiazide group with corresponding treatment. Blood and urine samples were collected from the rats for measurement of serum Na(+), urine volume and urine osmolality during medication. Semi-quantitative RT-PCR was performed to measure kidney inner medullary AQP(2) and vasopressin V(2)-R mRNA. Western blotting was employed to detect kidney inner medullary AQP(2) protein expression. Urine AQP(2) concentration was measured by enzyme-linked immunosorbent assay (ELISA).
Urine volume and urinary AQP(2) excretion were both increased in rats treated with the 3 drugs as compared with that of the control group. However, urine osmolality was lower in furosemide group but higher inhydrochlorothiazide and antisterone groups than in the control group (P<0.05). The kidney inner medullary AQP(2) mRNA, V(2)-R mRNA and AQP(2) protein expression of furosemide group increased in comparison with that of the control group (Plt;0.05). In hydrochlorothiazide group, however, the above parameters were all decreased (Plt;0.05).
The three classes of diuretics can all increase the excretion of the urinary AQP(2) but have different effects on the inner medullary AQP(2) mRNA and protein expression in normal rats. Hydrochlorothiazide reduces kidney AQP(2) mRNA and protein expression, while furosemide increased kidney AQP(2) gene expression.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 07/2007; 27(6):802-4.
[show abstract][hide abstract] ABSTRACT: To investigate effects of captopril and losartan on the expression of kidney aquaporin-2 (AQP2) mRNA and the excretion of urine AQP2 in rats.
Thirty healthy rats were randomized into 3 groups, namely the control group, captopril group and losartan group, respectively. Blood and urine samples were collected from the rats for detecting serum Na(+), urine volume and urine osmolality in the course of medication. Urine AQP2 concentration was measured by enzyme-linked immunosorbent assay (ELISA). Semi-quantitative RT-PCR was performed for measurement of kidney inner medullary AQP2 and vasopressin V(2) receptor mRNA.
Urine volume was increased in rats of captopril and losartan groups as compared with that of the control group. However, urine osmolality was lower in captopril group than in the other two groups (P<0.05). RT-PCR revealed decreased quantity of the inner medullary AQP2 mRNA of the captopril group than that of the other two groups, but the quantity of V(2) receptor mRNA did not differ significantly between the 3 groups. Urine AQP2 concentration was significantly higher in captopril group than in the control (P<0.05) and losartan groups (P0<0.01).
Captopril can reduce the expression of the kidney inner medullary AQP2 mRNA and accelerate the excretion of the urine AQP2 in normal rats.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 04/2005; 25(4):391-4.
[show abstract][hide abstract] ABSTRACT: To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.
CMs cultured for 3 to 5 days in vitro were incubated with Ca(2+)-sensitive fluorescent indicator Fluo-3AM with light screening at 37 degrees celsius; with 5% CO(2) for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).
Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.
AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 04/2005; 25(3):274-6, 280.
[show abstract][hide abstract] ABSTRACT: To investigate the effects of advanced glycation end-products (AGEs) on cell cycle distribution and apoptosis in neonatal rat cardiac myocytes (CMs) cultured in vitro.
CMs already cultured for 3 to 5 d in vitro were continuously cultured with DMEM supplemented with 1% or 10% fetal bovine serum for 24 h, prior to exposure to AGE-modified human serum albumin (AGE-HSA, at 50 and 100 microg/ml) for 12, 24 and 48 h. Cells cultured in the same manner but without AGE-HSA treatment served as the control group. All cells were collected and analyzed by flow cytometry for cell cycle distribution and cell apoptosis, and the number of apoptotic body/nucleus of CMs was determined by in situ cell death detection kit (fluorescein).
AGEs had no obvious effects on cell cycle distribution in CMs, but could increase the number of apoptotic body/nucleus of CMs in a time-dependent manner to up to 0.55 and 1.23 times at 24 and 48 h respectively, as compared with the number at 12 h in control cells. AGEs at 50 and 100 microg/ml increased the number of apoptotic body/nucleus of CMs to various degrees at different time points: 0.74 and 1.21 times respectively at 12 h; 1.15 and 1.78 times at 24 h; 0.83 and 1.19 times at 48 h. The average number of apoptotic body/nucleus of the cells treated with 100 microg/ml AGEs at 12, 24, 48 h were 0.27, 0.29, 0.20 times respectively that of the cells with 50 microg/ml AGEs treatment.
AGEs do not affect the distribution of cell cycle but can impair neonatal rat CMs by increasing their apoptosis rate.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 02/2003; 23(1):9-11, 15.
[show abstract][hide abstract] ABSTRACT: To develop an efficient and stable enzyme-linked immunosorbent assay (ELISA) for detecting urinary aquaporin-2 (AQP2) water channel protein.
Rat AQP2 C-terminal peptides (CELHSPQSLPRGSKA) were synthesized and linked to KLH to prepare rabbit anti-AQP2 polyclonal antibodies, and IgG of the antibodies were labeled with horseradish peroxidase (HRP). Rat models of congestive heart failure (CHF) was established by ligation of the left coronary artery, in which both direct and sandwich ELISA for urinary AQP2 detection were tested.
Double antibody sandwich ELISA was able to detect urinary AQP2 as low as 15.625 pmol/ml with intra-and inter-assay coefficients of variance (CVs) of 4.65% and 14.05% respectively. Urinary AQP2 concentration determined by this assay showed significant positive relation to that by Western blot analysis in CHF rats.
Double antibody sandwich ELISA was successfully established to detect urine AQP2 in CHF rats, which is more efficient and simpler than Western blot analysis.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 07/2002; 22(6):486-9.