Publications (2)3.23 Total impact
Article: Electrochemical performances of electroactive nano-layered organic-inorganic perovskite containing trivalent iron ion and its use for a DNA biosensor preparation.[show abstract] [hide abstract]
ABSTRACT: A steady nano organic-inorganic perovskite hybrid with [H(2)3-AMP](3/2)Fe(CN)(6) (3-AMP = 3-methylaminopyridine) was prepared in the air. The structure is an unusual layered organic-inorganic type. The resulting hybrid enveloped in paraffin to prepare [H(2)3-AMP](3/2)Fe(CN)(6) paste electrode (HPE) shows good electrochemical activity and a couple of oxidation and reduction peaks with potential of cyclic voltammometry (CV) at around 440 mV and 30 mV. Compared with that on CPE, oxidation potential of Fe(CN)(6) (3-) on HPE shifts negatively 259.7 mV and that of reduction shifts positively 338.7 mV, which exhibits that [H(2)3-AMP](3/2)Fe(CN)(6) can accelerate the electron-transfer to improve the electrochemical reaction reversibility. Such characteristics of [H(2)3-AMP](3/2)Fe(CN)(6) have been employed to prepare the DNA biosensor. The single-strand DNA (ssDNA) and double-strand DNA (dsDNA) immobilized on HPE, respectively, can improve the square wave voltammometry (SWV) current and SWV potential shifts positively. The effect of pH was evaluated. And there is hybridization peak on SWV curve using HPE immobilized ssDNA in the complementary ssDNA solution. And HPE immobilized ssDNA can be utilized to monitor the DNA hybridization and detect complementary ssDNA, covering range from 3.24 x 10(-7) to 6.72 x 10(-5) g/mL with detection limit of 1.57 x 10(-7) g/mL. The DNA biosensor exhibits a good stability and reproducibility.International Journal of Analytical Chemistry 01/2010; 2010:419439.
Article: Angelica injection reduces cognitive impairment during chronic cerebral hypoperfusion through brain-derived neurotrophic factor and nerve growth factor.[show abstract] [hide abstract]
ABSTRACT: The current study investigated whether chronic cerebral hypoperfusion produced by permanent bilateral common carotid artery occlusion (2-vessel occlusion (2-VO)) induced cognitive impairment and whether angelica injections alleviated the impairment. Furthermore, the study examined whether 2-VO altered the expression patterns of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the hippocampus of rats and whether angelica injections attenuated the alteration. Rats were divided into four groups to receive either 2-VO surgery or sham surgery followed by either angelica injections or saline injections for eight weeks. Spatial learning in Morris water maze and the expression patterns of BDNF and NGF in the hippocampus of all rats were examined. The results showed that 2-VO significantly impaired spatial learning and memory, and angelica injections significantly reversed the learning and memory impairment. Furthermore, 2-VO resulted in significantly decreased BDNF protein, NGF protein, and NGF mRNA expression in the hippocampus. Angelica injections significantly attenuated the decreased expression. Moreover, spatial learning in Morris water maze was positively correlated to the expression of BDNF and NGF in the hippocampus. Thus, angelica injections might alleviate cognitive impairment during chronic cerebral hypoperfusion through BDNF and NGF.Current neurovascular research 03/2008; 5(1):13-20. · 3.23 Impact Factor