Guojun Yin

Publications (7)9 Total impact

• Article: The protective effect of silymarin on the carbon tetrachloride (CCl(4))-induced liver injury in common carp (Cyprinus carpio).

  Rui Jia, Liping Cao, Jinliang Du, Pao Xu, Galina Jeney, Guojun Yin
  [show abstract] [hide abstract]
  ABSTRACT: Silymarin, a mixture of bioactive flavonolignans from the milk thistle (Silybum marianum), is traditionally used in herbal medicine to defend against various hepatotoxic agents. The aim of the present study was to evaluate the protective effect of silymarin against carbon tetrachloride (CCl(4))-induced liver injury in fish. Common carp, with an average initial weight of 17.0 ± 1.1 g, were fed diet containing four doses of silymarin (0, 0.1, 0.5, and 1 g/kg diet) for 60 d. Fish were then given an intraperitoneal injection of CCl(4) (30% in arachis oil) at a dose of 0.5 ml/kg body weight. At 72 h after CCl(4) injection, blood and liver samples were collected for the analyses of serum biochemical parameters, liver index, peroxidation product, glutathione, and antioxidant enzyme activities. The results showed that administration of silymarin at 0.5 and 1 g/kg diet for 60 d prior to CCl(4) intoxication significantly reduced the elevated activities of glutamate pyruvate transaminase, glutamate oxalate transaminase, lactate dehydrogenase (LDH), and increased the reduced levels of total protein and albumin in the serum. The reduced levels of liver index, superoxide dismutase, glutathione peroxidase, catalase, glutathione, and total antioxidant capacity were markedly increased, and malondialdehyde formation was significantly restrained in the liver. However, these parameters, except LDH, were not significantly changed in fish fed with silymarin at 0.1 g/kg diet. Based on the results, it can be concluded that silymarin has protective effect against CCl(4)-induced hepatotoxicity in fish. It is suggested that silymarin may be used as a hepatoprotective agent to prevent liver diseases in fish.
  In Vitro Cellular & Developmental Biology - Animal 02/2013; · 1.31 Impact Factor
• Article: In vitro and in vivo hepatoprotective and antioxidant effects of Astragalus polysaccharides against carbon tetrachloride-induced hepatocyte damage in common carp (Cyprinus carpio).

  Rui Jia, Liping Cao, Pao Xu, Galina Jeney, Guojun Yin
  [show abstract] [hide abstract]
  ABSTRACT: The present study is aiming at evaluating the hepatoprotective and antioxidant effects of Astragalus polysaccharide (APS) on the carbon tetrachloride (CCl(4))-induced hepatocyte and liver injury in common carp in vitro and in vivo. In vitro, APS (200, 400 and 800 μg/ml) was added to the carp primary hepatocytes before (pre-treatment), after (post-treatment) and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4) at 8 mM in the culture medium. APS at concentrations of 200, 400 and 800 μg/ml significantly improved cell viability and inhibited the elevation of glutamate pyruvate transaminase (GPT), glutamate oxalate transaminase (GOT), lactate dehydrogenase (LDH) and malondialdehyde (MDA) and significantly increased the reduced level of superoxide dismutase (SOD). In vivo administration of APS at the doses of 1.5 and 3 g/kg in the diet for 60 days prior to CCl(4) intoxication significantly reduced the elevated activities of GPT, GOT and LDH and increased the reduced levels of total protein and albumin in the serum; meanwhile, the reduced levels of SOD, glutathione and total antioxidant capacity (T-AOC) were markedly increased and the MDA formation was significantly inhibited in liver tissue. Overall results proved the hepatoprotective action of APS, which is likely related to its antioxidant activity. The results support the use of APS as a hepatoprotective and antioxidant agent in fish.
  Fish Physiology and Biochemistry 11/2011; 38(3):871-81. · 1.53 Impact Factor
• Article: Hepatoprotective and antioxidant effects of Glycyrrhiza glabra extract against carbon tetrachloride (CCl(4))-induced hepatocyte damage in common carp (Cyprinus carpio).

  Guojun Yin, Liping Cao, Pao Xu, Galina Jeney, Miki Nakao, Chengping Lu
  [show abstract] [hide abstract]
  ABSTRACT: The present study is aiming at evaluating the hepatoprotective and antioxidant effects of Glycyrrhiza glabra extract (2.5, 5 and 10 μg/ml) on the carbon tetrachloride (CCl(4))-induced carp hepatocyte damage in vitro. Glycyrrhiza glabra extract was added to the carp primary hepatocytes before (pre-treatment), after (post-treatment) and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4). CCl(4) at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT) and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment (5 μg/ml) and pre- and post-treatment (5 and 10 μg/ml) of the hepatocytes with Glycyrrhiza glabra extract significantly reduced the elevated levels of LDH, GOT, GPT and MDA and increased the reduced levels of SOD and GSH-Px by CCl(4); post-treatment of the hepatocytes with Glycyrrhiza glabra extract at 5 μg/ml reduced the GPT and GOT levels and increased the GSH-Px level, but had no effect on the other parameters at all the studied concentrations. The results support the use of Glycyrrhiza glabra extract as a hepatoprotective and antioxidant agent in fish.
  Fish Physiology and Biochemistry 03/2011; 37(1):209-16. · 1.53 Impact Factor
• Article: Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio.

  Guojun Yin, Liping Cao, Pao Xu, Galina Jeney, Miki Nakao
  [show abstract] [hide abstract]
  ABSTRACT: The present study aims to evaluate the hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract on the carbon tetrachloride (CCl(4))-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. H. sabdariffa extract (100, 200, and 400 μg/mL) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4). CCl(4) at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment and pre- and post-treatment of the hepatocytes with H. sabdariffa extract significantly reduced the elevated levels of LDH, GOT, GPT, and MDA and increased the reduced activities of SOD and GSH-Px in a dose-dependent manner; post-treatment did not show any protective effect. The results suggest that H. sabdariffa extract can be potentially used for preventing rather than curing liver diseases in fish.
  In Vitro Cellular & Developmental Biology - Animal 11/2010; 47(1):10-5. · 1.31 Impact Factor
• Article: Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila.

  Guojun Yin, L Ardó, K D Thompson, A Adams, Z Jeney, G Jeney
  [show abstract] [hide abstract]
  ABSTRACT: The effect of Chinese herbs (Astragalus radix and Ganoderma lucidum) on immune response of carp was investigated. Fish were fed diets containing Astragalus (0.5%), Ganoderma (0.5%) and combination of two herbs (Astragalus 0.5% and Ganoderma 0.5%) for 5 weeks. Other groups of fish were vaccinated (i.p.) against Aeromonas hydrophila/Aeromonas salmonicida (Shering Plough, Essex, U.K.) at the beginning of the experiment and fed the same diets as described above. Control fish (negative control) and fish vaccinated only (positive control) were fed basal diets without supplements of herbs. The respiratory burst activity, phagocytosis, lysozyme activity and circulatory antibody titres in plasma were monitored. Following 5 weeks after feeding, fish were infected with A. hydrophila and mortalities were recorded. The results of this study showed that feeding non-vaccinated and vaccinated carp with combination of Astragalus and Ganoderma stimulated respiratory burst activity, phagocytosis of phagocytic cells in blood and lysozyme and circulatory antibody titres in plasma in vaccinated carp. Fish challenged with A. hydrophila had variable survival. The best survival (60%) was in vaccinated group fed with both herbs, while almost 90% of control fish (negative control) and 60% of fish vaccinated only (positive control) died.
  Fish &amp Shellfish Immunology 10/2008; 26(1):140-5. · 3.32 Impact Factor
• Article: Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila

  László Ardó, Guojun Yin, Pao Xu, László Váradi, Gábor Szigeti, Zsigmond Jeney, Galina Jeney
  [show abstract] [hide abstract]
  ABSTRACT: The effect of two Chinese medicinal herbs (Astragalus membranaceus and Lonicera japonica) and boron on non-specific immune response of Nile tilapia (Oreochromis niloticus) was investigated. Five diet variations in addition to a control diet (without herbs or boron) were used. These contained 0,1% Astragalus (with and without 0,05% boron), 0,1% Lonicera (with or without 0,05% boron) and a mixture of the two herbs with 0,05% boron. The diets were fed for four weeks, and respiratory burst and phagocytic activities of blood leukocytes; plasma lysozyme, total protein and total immunoglobulin level were measured weekly. After four weeks of feeding, fish were infected with Aeromonas hydrophila and mortalities were recorded. Results of this study showed that feeding tilapia with two herbs alone or in combination significantly enhanced phagocytic and respiratory burst activity of blood phagocytic cells. They had a moderate effect on the plasma lysozyme level and no effect on plasma total protein and total immunoglobulin level. Both herbs reduced the mortality following A. hydrophila infection. The lowest mortality was observed in the group fed with the combination of both herbs and boron. Combination of the herbs and boron could even more increase the survival rate of infected fish. It can be concluded that Astragalus and Lonicera extracts and boron supplementation added to fish feed can act as immunostimulants and enhance the immune response and disease resistance of cultured fish.
  Aquaculture.
• Article: Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus

  Guojun Yin, Galina Jeney, Timea Racz, Pao Xu, Xie Jun, Zsigmond Jeney
  [show abstract] [hide abstract]
  ABSTRACT: The effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus, was investigated. Three-month-old tilapia with an average initial weight of 62.8 ± 5.40 g were fed diets containing four doses of Astragalus radix (0; 0.1; 0.5 and 1.0%) and four doses of Scutellaria radix (0; 0.1; 0.5 and 1.0%) for 4 weeks. Respiratory burst activity (extracellular superoxide anion production), phagocytosis by isolated blood leukocytes (neutrophils and monocytes) and lysozyme activity in plasma were investigated 1, 2, 3 and 4 weeks after feeding. Both Astragalus radix and Scutellaria radix can modulate the innate immune system of tilapia. The results of this study showed that feeding tilapia with 0.1 and 0.5% Astragalus radix for 1 week enhanced lysozyme activity and for 3 weeks stimulated phagocytosis by phagocytic blood cells, however, respiratory burst activity of phagocytic cells was not increased. On the other hand, inhibition of phagocytosis and respiratory burst activity was found when tilapia was fed with medium and high doses of Scutellaria radix, but not lysozyme activity. There were no significant differences among groups fed with different doses of both herbs. The optimal doses of Astragalus were 0.1% and 0.5% with an optimal feeding period of 3 weeks. Further work is needed to establish the stimulatory dose and optimal time of feeding of Scutellaria.
  Aquaculture.