Guochun Liao

Publications (2)17.61 Total impact

• Source

  Available from: ncbi.nlm.nih.gov

  Article: The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes.

  Hugo J Bellen, Robert W Levis, Guochun Liao, Yuchun He, Joseph W Carlson, Garson Tsang, Martha Evans-Holm, P Robin Hiesinger, Karen L Schulze, Gerald M Rubin, Roger A Hoskins, Allan C Spradling
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  ABSTRACT: The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.
  Genetics 07/2004; 167(2):761-81. · 4.01 Impact Factor
• Article: The Drosophila gene collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes.

  Mark Stapleton, Guochun Liao, Peter Brokstein, Ling Hong, Piero Carninci, Toshiyuki Shiraki, Yoshihide Hayashizaki, Mark Champe, Joanne Pacleb, Ken Wan, Charles Yu, Joe Carlson, Reed George, Susan Celniker, Gerald M Rubin
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  ABSTRACT: Collections of full-length nonredundant cDNA clones are critical reagents for functional genomics. The first step toward these resources is the generation and single-pass sequencing of cDNA libraries that contain a high proportion of full-length clones. The first release of the Drosophila Gene Collection Release 1 (DGCr1) was produced from six libraries representing various tissues, developmental stages, and the cultured S2 cell line. Nearly 80,000 random 5' expressed sequence tags (5' expressed sequence tags [ESTs]from these libraries were collapsed into a nonredundant set of 5849 cDNAs, corresponding to ~40% of the 13,474 predicted genes in Drosophila. To obtain cDNA clones representing the remaining genes, we have generated an additional 157,835 5' ESTs from two previously existing and three new libraries. One new library is derived from adult testis, a tissue we previously did not exploit for gene discovery; two new cap-trapped normalized libraries are derived from 0-22-h embryos and adult heads. Taking advantage of the annotated D. melanogaster genome sequence, we clustered the ESTs by aligning them to the genome. Clusters that overlap genes not already represented by cDNA clones in the DGCr1 were analyzed further, and putative full-length clones were selected for inclusion in the new DGC. This second release of the DGC (DGCr2) contains 5061 additional clones, extending the collection to 10,910 cDNAs representing >70% of the predicted genes in Drosophila.
  Genome Research 08/2002; 12(8):1294-300. · 13.61 Impact Factor