Publications (2)0 Total impact
Article: [Comparative Proteomic Analysis of Human Lung Adenocarcinoma Cisplatin-resistant Cell Strain A549/CDDP.].[show abstract] [hide abstract]
ABSTRACT: Chemotherapy plays an important role in the comprehensive therapy of lung cancer. However, the drug-resistance often causes the failure of the chemotherapy. The aim of this study is to identify differently expressed protein before and after cisplatin resistance of human lung adenocarcinoma cell A549 by proteomic analysis. Cisplatin-resistant cell strain A549/CDDP was established by combining gradually increasing concentration of cisplatin with large dosage impact. Comparative proteomic analysis of A549 and A549/CDDP were carried out by means of two-dimensional gel electrophoresis. The differentially expressed proteins were detected and identified by MALDI-TOF mass spectrometry. Eighty-two differentially expressed proteins were screened by analysis the electrophoretic maps of A549 and A549/CDDP. Six differential proteins were analyzed by peptide mass fingerprinting. Glucose regulating protein 75, ribosomal protein S4, mitochondrial ATP synthase F1 complex beta subunit and immunoglobulin heavy chain variable region were identified. All four differentially expressed proteins were over-expressed in A549/CDDP, whereas low-expressed or no-expressed in A549. These differentially expressed proteins give some clues to elucidate the mechanism of lung cancer cell resistant of cisplatin, providing the basis of searching for potential target of chemotherapy of lung cancer.Zhongguo fei ai za zhi = Chinese journal of lung cancer 11/2009; 12(11):1155-8.
Article: [Expression of Copper-Transporting P-Type Adenosine Triphosphatase (ATP7B) Correlates with Cisplatin-Resistance in Human Lung Adenocarcinoma Cell Line A549.].[show abstract] [hide abstract]
ABSTRACT: Cisplatin is the basic chemotherapy agent of lung carcinoma, and cisplatin-resistance mostly leads to the failing of chemotherapy in the patients. The aim of this study is to investigate the relationship between the expression of copper-transporting P-type adenosine triphosphatase (ATP7B) and cisplatinresistance in different cisplatin-resistant A549 cells. Three differently cisplatin-resistant A549 sublines (A549/DDP0.5, A549/DDP1.0, A549/DDP2.0) were established from their parental human lung adenocarcinoma cell line A549 which was sensitive to cisplatin, by gradually increasing concentration of cisplatin. The resistance indexes of all the sublines were checked by MTT method. The levels of ATP7B mRNA and protein were measured by RT-PCR and Western Blot respectively in all the cell lines. The resistance indexes of the three cisplatin-resistant sublines were 1.7, 3.2 and 5.2 respectively (P <0.001), and the levels of ATP7B mRNA were 1.6, 4.9 and 10.1 folds compared to the parental A549 cells (P <0.001). Meanwhile, expression of ATP7B protein was enhanced in the three cisplatin-resistant sulines corresponding to their cisplatin-resistance. Expression of ATP7B correlates with cisplatin-resistance in A549 cells, and maybe ATP7B contributes to acquisition of cisplatin-resistance.Zhongguo fei ai za zhi = Chinese journal of lung cancer 04/2009; 12(4):350-3.