[Show abstract][Hide abstract] ABSTRACT: As an alternative to gas chromatography, the enzymatic UV assay of ethylene glycol is often used by emergency laboratories. Many variants of this technique have been published, all based on the reaction between NAD(+) and ethylene glycol in the presence of glycerol dehydrogenase (EC 220.127.116.11). We show that other alpha-diols interfere in this reaction. Some of them, like 2,3-butanediol, give false positive reactions; whereas other diols, e.g. glycerol and propylene glycol, interfere only when ethylene glycol is present in the sample and lower the ethylene glycol response; these interferents are of particular concern because some parenteral drugs used in emergency situations contain glycerol or propylene glycol in their vehicle. This drawback has hitherto been largely underestimated, and we think that ethylene glycol results obtained with these enzymatic techniques should be interpreted with caution, even if the sample is pre-treated with glycerokinase (EC 18.104.22.168); this pre-treatment effectively corrects the glycerol interference but not that of propylene glycol.
European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 09/1996; 34(8):651-4.
[Show abstract][Hide abstract] ABSTRACT: A new CEDIA assay for the measurement of digoxin in serum on random access analyzers was evaluated by twelve laboratories in Europe and the United States. Studies on the analytical range, reproducibility, calibration stability, recovery in controls, interlaboratory comparability, comparability with routine methods, and the effect of various interfering factors have been performed and the results are presented in this paper. The analytical performance was comparable to that of routine methods provided the manual pipetting step for pre-incubation was performed with accurate pipettes. A major advantage of the CEDIA Digoxin assay in terms of convenience is the simple two-point calibration procedure. Moreover, digoxin can be determined within 15 minutes after receiving the samples on random access analyzers like Boehringer Mannheim/Hitachi analysis systems. Thus, the CEDIA Digoxin assay represents an attractive alternative to the measurement of digoxin on dedicated immunochemical assay systems.
Wiener klinische Wochenschrift. Supplementum 02/1992; 191:69-73.
[Show abstract][Hide abstract] ABSTRACT: In which fields of medicine, and in which journals does one publish the majority of articles which concern clinical biochemistry? We are trying to reply to this question by studying the basis of the biographical data developed in our laboratory, proceeding from the perusal of 290 journals and representing 16 802 articles stored from 1978 to 1983. Clinical Chemistry is the most important journal as far as number of articles is concerned, but less articles arises from journals of clinical biochemistry than from those of general medicine and internal medicine. The theme "lipoproteins and atherosclerosis" is the most prolific. It is studied in more detail. In a general way, a theme is rarely covered in a satisfactory manner with 5 journals or less. We think that, faced with the great dispersion of the same theme across the medical literature, our results will help the biologist to make a choice both from the point of view of contract and biographical research.
Annales de biologie clinique 02/1985; 43(2):109-16. · 0.30 Impact Factor