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Chao Wang,
Ying Wang,
Xiaoyan Du,
Lin Zeng, Gang Dong,
Yanhua Wu,
Jing Lu,
Deyou Wei,
Xi Zhu,
Guosheng Liu,
Taiyun Zhao,
Zhenwen Chen
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ABSTRACT: To the Editor: In the People's Republic of China, <3,000 persons die of rabies each year; most were infected by dog bites (1). Since 2000, the dog population in Beijing has increased dramatically, and the exact vaccination coverage and immunization status of dogs are not known. During 2006-2009, to assist with governmental rabies control, Fengtai District was selected as a geographically representative area in Beijing in which to conduct a survey of rabies antibody titers in domestic dogs.
Emerging Infectious Diseases 06/2011; 17(6):1129-30. · 6.79 Impact Factor
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Xiao Yan Du,
Xiang Dong Zhu, Gang Dong,
Jing Lu,
Ying Wang,
Lin Zeng,
Tai Yun Zhao,
Hua Hu Ye,
Rui Sheng Li,
Jie Ying Bai,
Zhen Wen Chen
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ABSTRACT: Mongolian gerbils (Meriones unguiculatus) have an incomplete circle of Willis (CoW), as a result of which approximately 30-40% of these animals develop focal cerebral ischemia after unilateral carotid occlusion (UCO). There are four types of patterns of the anterior and posterior communicating arteries (ACoAs and PCoAs, respectively) of the CoW and they determine the severity of the ischemic symptoms. We used 398 gerbils from five generations, including a selectively bred ischemia-prone group, to investigate post-UCO ischemic symptoms and possible correlations of ACoA and PCoA patterns between parents and their progeny. We observed that if the parents had complete ACoAs, their progeny also had complete ACoAs, and we found significant differences when the parents' ACoAs were incomplete: in 60.4% of offspring the type of ACoA was consistent with that of the mother and in 48.2% it was consistent with that of the father. The severity of the neurological symptoms after UCO was significantly related to the patterns of the ACoAs when PCoAs were absent. The proportion of UCO ischemia in gerbils with incomplete ACoAs was significantly higher than in those with complete ACoAs. After selectively breeding five generations, the proportion of UCO ischemia increased from 40% in the F1 animals to 75% in the F5 animals. Our results suggest that variations in the CoW are genetic and demonstrate that we successfully established an ischemia-prone group of gerbils.
ILAR journal / National Research Council, Institute of Laboratory Animal Resources 02/2011; 52(1):E1-7. · 2.33 Impact Factor
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ABSTRACT: Our early study found that goat spermatozoa could spontaneously take up foreign DNA and vary in capabilities of spermatozoa from different donors to bind and internalize exogenous DNA. In this study, three goats with considerable differences of capability were used to investigate the effect of exogenous DNA on goat spermatozoa, and feasibility and efficiency of transgenic embryo production by sperm-mediated gene transfer method. The viability, acrosomal reaction frequencies and cleavages were decreased in the groups co-cultured with exogenous DNA, compared with the control groups, and the range of decrease was correlated with the capability of sperm cells up-take foreign DNA. After fertilizing with co-cultured spermatozoa, GFP gene was introduced into oocytes and expressed in early embryos. However, different efficiencies of transgenic embryos appeared in sperm donors (P<0.05). GFP gene was detected in 16.2% (25/154), 5.3% (4/76), and 0% (0/36) embryos, respectively, when high, middle and low capability of sperm donors were used. But only 6.5% (10/154) embryos from high capability sperm donor expressed GFP. Our results demonstrate that selecting high capability of sperm donor is a key step for improving efficiency of sperm mediated-gene transfer method. However, the adverse influence of foreign DNA on spermatozoa needs to be further studied.
Hereditas (Beijing) 11/2008; 30(11):1421-6.
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ABSTRACT: Microsatellite DNA was used to monitor the gene inheritance of successive generations of rats. It is useful in selective breeding to quickly bring genes to homozygosity and to shorten the cultivation periods of new inbreeding of rats. Thirty microsatellite DNA loci were PCR amplified to analyze the DNA polymorphism in generational crosses between the outbred Wistar and SD rats. Rats in F generations with a moderate to high coefficient of similarity with the female F0 SD rats were crossed. All loci in the F2 generation were heterozygous and polymorphic. By F9, 27 loci have become homozygotic, with a homozygosity rate of 90%. The coefficient of similarity tended to increase at a rate of 6%-20% with each successive generation. Skin grafting confirmed no rejection among the F9 generation of rats. Therefore we have established a novel method for rapid cultivation of inbred rats.
Hereditas (Beijing) 08/2006; 28(7):821-4.
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ABSTRACT: Eleven BALB/c mouse strains of Beijing, Shanghai, Shenyang, Haerbin, Guangzhou, Chongqing and Changchun were monitored in order to assure the genetic quality of inbred BALB/c mouse strains in China and to estimate the credibility of microsatllite markers. Fourteen microsatellites loci on different chromosomes were investigated by PCR analysis. It showed that all these microsatellites DNA loci displayed single allelic gene band in mouse strains of Beijing, Shanghai and Haerbin. But the mice came from Shenyang, Guangzhou, Chongqing and Changchun had polymorphisms or heterozygosis, among which the Shenyang and Changchun strains showed polymorphisms and heterozygosis at two separate loci. Four loci showed polymorphisms or heterozygosis in one of the Guangzhou mouse strains. The Chongqing strains showed polymorphisms and heterozygosis at seven loci, including the D10Mit180 locus as compared with the Shanghai strains.
Hereditas (Beijing) 12/2004; 26(6):845-8.
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ABSTRACT: The objectives of this study are to establish microsatellite loci for the Mongolian gerbil based on mouse microsatellite DNA sequences and to investigate genetic variation in the laboratory gerbil (Capital Medical University, CMU) and 2 wild gerbil populations (from Yin Chuan city [YIN] and the Hohehot Municipality [HOH]). In total, 536 mouse microsatellite markers were chosen to identify polymorphic dinucleotide repeat loci in the gerbil by cross-amplification. Of these markers, 313 (58.39%) have been discretely amplified from the CMU laboratory gerbil and been sequenced. Of the 313 sequenced markers, 130 were confirmed as simple sequence repeat (SSR) loci in the gerbil. In total, 6 of those newly identified loci plus 6 identified in previous reports were used to estimate the genetic polymorphism for 30 laboratory gerbils and 54 wild gerbils (27 each of the HOH and YIN groups). A total of 29 alleles were observed in the 3 populations, and 11 of 12 loci (91.67%) are polymorphic markers. Nei's standard genetic distances of 0.0592 (CMU vs. HOH) and 0.1033 (CMU vs. YIN) were observed. The averages of observed versus expected heterozygosity are 0.5231/0.4008, 0.5051/0.3882, and 0.4825/0.3665 for the YIN, HOH, and CMU populations, respectively. These results show that cross-amplification using mouse microsatellite primers is an efficient way to identify gerbil SSR loci. By using these 12 selected markers, we have demonstrated that genetic variation level within the CMU population is higher than that has been reported previously and are comparable with the levels found in 2 wild populations.
The Journal of heredity 101(6):710-6. · 2.05 Impact Factor
