Ed. A. O'Rear

Rice University, Houston, Texas, United States

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Publications (2)8.14 Total impact

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    ABSTRACT: Erythrocytes exposed to subhemolytic shear stress in vitro exhibit decreased deformability as determined by a filtration method. Intracellular calcium content of these cells has been measured by atomic absorption spectroscopy and found to be 35 and 55% higher than controls (0.0157 ╬╝mol/ml packed red blood cells) after shear stress levels of 100 and 130 N/cm2, respectively. These alterations occur without significant changes in ATP level, intracellular magnesium content, cell volume, or morphology, and without large associated sodium and potassium fluxes. Results indicate that calcium may be responsible for or associated with changes in the viscoelastic properties of the red cell membrane caused by sublytic mechanical trauma.
    Biochimica et Biophysica Acta 11/1982; 691(2):274-80. DOI:10.1016/0005-2736(82)90416-3 · 4.66 Impact Factor
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    ABSTRACT: As calcium has increasingly been the object of study in erythrocyte physiology, we reviewed the current methodologies for determination of calcium by atomic absorption spectrometry. The published normal values for erythrocyte calcium vary from 5 to 500 mumol/liter of packed cells. A method based on Harrison and Long's determination of calcium is presented and shows normal red cell calcium concentration to be 0.0149 +/- 0.0023 mumol/ml of packed red cells. The influence of temperature and type of crucible used in ashing red cells is assessed. The method of additions is employed to corroborate our results.
    American Journal of Hematology 11/1981; 11(3):283-92. DOI:10.1002/ajh.2830110309 · 3.48 Impact Factor