Frédéric Sailhan

Université René Descartes - Paris 5, Lutetia Parisorum, Île-de-France, France

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Publications (41)81.32 Total impact

  • A. Courvoisier, Frédéric Sailhan, O Laffenêtre, L Obert, GTEBO
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    ABSTRACT: Purpose Bone morphogenetic proteins (BMP) are recombinant osteoinductive proteins with their primary role being to promote bone formation. The off-label use of BMP in orthopaedic surgery has dramatically increased. However, reports of complications with BMP have emerged, and the safety of these proteins in orthopaedics is questioned. The purpose of this review was to evaluate safe situations in which BMP should be used and situations in which their use should be restricted. Method We recorded all studies from PubMed database from 2002 (date of first authorisation for both BMPs) until January 2014 using “BMP” or “bone morphogenetic protein”. Then we screened and extracted all studies dealing with orthopaedic surgery. All situations in which BMP were used, even cases reports, were considered, and complications reported were then listed.
    International Orthopaedics 09/2014; · 2.32 Impact Factor
  • Clémence Palazzo, Frédéric Sailhan, Michel Revel
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    ABSTRACT: Scheuermann's disease is a juvenile osteochondrosis of the spine. It is a disease of the growth cartilage endplate, probably due to repetitive strain on the growth cartilage weakened by a genetic background. The radiographic aspects are related to the vertebral endplate lesions and include vertebral wedging, irregularity of the vertebral endplate, and Schmorl's node (intraossous disk herniation). Disc alterations are frequent and may be secondary to dysfunction of the disc-vertebra complex. The definitions of Scheuermann's disease are varied; it can refer to the classical form of juvenile kyphosis, described by Scheuermann as well as asymptomatic radiographic abnormalities. Lumbar involvement is probably as frequent as the thoracic form and might be more painful. The first-line treatment is medical and includes rehabilitation and bracing. The earlier the start of treatment, the better the outcome, which highlights the importance of early diagnosis. Surgery is uncommon and must be limited to severe involvement after failure of conservative treatment. The natural history of Scheuermann's disease is unknown, but it might be associated with increased risk of back pain. The evolution of thoracolumbar and lumbar disease is unknown.
    Joint, bone, spine: revue du rhumatisme 01/2014; · 2.25 Impact Factor
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    ABSTRACT: El condroblastoma es un tumor óseo benigno del niño, del adolescente o del adulto joven; representa el 1% de todos los tumores óseos. De localización epifisaria o epifisometafisaria, esta lesión se desarrolla habitualmente a partir de centros secundarios de osificación cercanos a la rodilla, al hombro y a la cadera. En la radiografía suele observarse una lesión osteolítica epifisaria en contacto con el cartílago de crecimiento y con calcificaciones en su interior. El análisis anatomopatológico de una muestra obtenida por biopsia o raspado-biopsia proporciona el diagnóstico de certeza. El tratamiento del condroblastoma es quirúrgico y consiste en el raspado del tumor y el llenado de la cavidad residual. La recidiva es frecuente (hasta el 35% en algunas series) y necesita un nuevo raspado o, en ocasiones, la resección completa de la lesión.
    EMC - Aparato Locomotor. 01/2014; 47(1):1–9.
  • Frédéric Sailhan
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    ABSTRACT: Several surgical techniques have been described for the treatment of degenerative lumbar disc disease. The allow reaching the disc space from the anterior, posterior or lateral aspect of the spine. Posterior Lumbar Interbody Fusion, Transforaminal Lumbar Interbody Fusion and Anterior Lumbar Interbody Fusion techniques are described and compared. The interest of minimally invasive retractors is also discussed in these surgical procedures.
    Revue du Rhumatisme Monographies 09/2013; 80(4):252–259.
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    ABSTRACT: Immobilization of bone morphogenetic proteins (BMP) onto material surfaces is a promising, but still challenging, strategy for achieving dependable and consistent osseointegration of long-term metal implants. In the present study, we have developed an osteoinductive coating of a porous titanium implant using biomimetic polyelectrolyte multilayer (PEM) films loaded with BMP-2. The amount of BMP-2 loaded in these films was tuned - over a large range - depending on the cross-linking extent of the film and of the BMP-2 initial concentration. The air-dried PEM films were stable for at least one year of storage at 4 °C. In addition, they resisted exposure to γ-irradiation at clinically approved doses. The preservation of the growth factor bioactivity upon long-term storage and sterilization were evaluated both in vitro (using C2C12 cells) and in vivo (in a rat ectopic model) for the perspective of industrial and clinical development. BMP-2 loaded in dried PEM films exhibited shelf-life stability over one year. However, their bioactivity in vitro decreased from 50 to 80% after irradiation depending on the γ-irradiation dose. Remarkably, the in vivo studies showed that the osteoinductive potential of BMP-2 contained in PEM-coated Ti implants was fully preserved after air-drying of the implants and sterilization at 25 kGy. Film drying or irradiation did not affect the amount of new bone tissue formation. This "off-the-shelf" novel technology of functionalized implants opens promising applications in prosthetic and tissue engineering fields.
    Biomaterials 05/2013; · 8.31 Impact Factor
  • Clémence Palazzo, Frédéric Sailhan, Michel Revel
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    ABSTRACT: Scheuermann's disease is a form of juvenile osteochondrosis of the spine. It is a disease of the growth cartilage end plate, which is probably due to repetitive strains on the growth cartilage, which has been weaken by a genetic background. The radiologic aspects are related to the vertebral end plate lesions. They are: vertebral cuneiformisation, irregularity of the vertebral end plate, Schmorl's node (intraossous disk herniation). Discal alterations are frequent, and may be secondary to a dysfunctionment of the disc-vertebra complex. The definitions of Scheuermann's disease vary; it can refer to the classical form of a juvenile kyphosis described by Scheuermann himself, as well as asymptomatic radiographic abnormalities. Lumbar involvement is probably as frequent as the thoracic form, and might be more painful. The treatment of Scheuermann's disease is first of all medical, including physiotherapy and bracing. Earlier the treatment is started, better the outcome is, highlighting the interest of an early diagnosis. Surgery is very rare, and must be limited to severe involvement and/or when the conservative treatment has failed. The natural history of Scheuermann's disease is unknown, but it might be associated with a higher risk to develop back pain. The evolution of thoracolumbar and lumbar involvement is unknown.
    Revue du Rhumatisme Monographies 01/2013;
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    ArgoSpine News & Journal 12/2012; 24(3-4).
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    ABSTRACT: Macrophages exert either beneficial or deleterious effects on tissue repair, depending on their activation/polarization state. They are crucial for adult skeletal muscle repair, notably by acting on myogenic precursor cells. However, these interactions have not been fully characterized. Here, we explored both in vitro and in vivo, in human, the interactions of differentially activated MPs with muscle precursor cells (MPCs) during adult myogenesis and skeletal muscle regeneration. We showed in vitro that through the differential secretion of cytokines and growth factors, pro-inflammatory macrophages inhibited MPC fusion while anti-inflammatory macrophages strongly promoted MPC differentiation by increasing their commitment into differentiated myocytes and the formation of mature myotubes. Furthermore, the in vivo time course of expression of myogenic and macrophage markers was studied in regenerating human healthy muscle after damage. We observed that regenerating areas containing proliferating MPCs were preferentially associated with macrophages expressing pro-inflammatory markers. In the same muscle, regenerating areas containing differentiating myogenin positive MPCs were preferentially coupled to macrophages harbouring anti-inflammatory markers. These data demonstrate for the first time in human that macrophages sequentially orchestrate adult myogenesis during regeneration of damaged skeletal muscle. These results support the emerging concept that inflammation, through macrophage activation, controls stem cell fate and coordinates tissue repair.
    Stem Cells 11/2012; · 7.70 Impact Factor
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    ABSTRACT: Efficient delivery of growth factors is one of the great challenges of tissue engineering. Polyelectrolyte multilayer films (PEM) made of biopolymers have recently emerged as an interesting carrier for delivering recombinant human bone morphogenetic protein 2 (rhBMP-2 noted here BMP-2) to cells in a matrix-bound manner. We recently showed that PEM made of poly(l-lysine) and hyaluronan (PLL/HA) can retain high and tunable quantities of BMP-2 and can deliver it to cells to induce their differentiation in osteoblasts. Here, we investigate quantitatively by Fourier transform infrared spectroscopy (FTIR) the secondary structure of BMP-2 in solution as well as trapped in a biopolymeric thin film. We reveal that the major structural elements of BMP-2 in solution are intramolecular β-sheets and unordered structures as well as α-helices. Furthermore, we studied the secondary structure of rhBMP-2 trapped in hydrated films and in dry films since drying is an important step for future applications of these bioactive films onto orthopedic biomaterials. We demonstrate that the structural elements were preserved when BMP-2 was trapped in the biopolymeric film in hydrated conditions and, to a lesser extent, in dry state. Importantly, its bioactivity was maintained after drying of the film. Our results appear highly promising for future applications of these films as coatings of biomedical materials, to deliver bioactive proteins while preserving their bioactivity upon storage in dry state.
    Biomacromolecules 09/2012; · 5.37 Impact Factor
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    ABSTRACT: Los tumores óseos malignos primarios son tumores infrecuentes. Su baja incidencia y la necesidad de un tratamiento multidisciplinario, a cargo de médicos con experiencia en esta afección, imponen el traslado de estos pacientes a los centros de referencia o, como mínimo, el análisis de los casos en reuniones de concertación multidisciplinaria especializada. La resección quirúrgica de los tumores malignos óseos debe ser amplia, es decir, habrá que dejar en contacto con el tumor una capa de tejido sano (margen). En más del 80% de los casos es posible reconstruir el segmento óseo o la articulación y, por tanto, conservar el miembro. Las diáfisis se reconstruyen con preferencia mediante una combinación de auto y aloinjerto con osteosíntesis. En la mayoría de los casos, las articulaciones se reconstruyen con una prótesis masiva o se efectúa una reconstrucción compuesta (prótesis rodeada por un aloinjerto). Cuando, además de la articulación, la resección incluye los músculos periarticulares, se prefiere la artrodesis. Las resecciones de la pelvis, si se limitan al ala ilíaca o al marco isquiopúbico, no necesitan reconstrucción. En cambio, las resecciones de la región acetabular y del ala ilíaca, que interrumpen la continuidad del anillo pélvico, imponen la reconstrucción a efectos de lograr un resultado funcional aceptable. La reconstrucción de la cadera tras la resección del acetábulo es mucho más difícil. En caso de resección de la región acetabular, sola o asociada a la del marco isquiopúbico, los autores de este artículo prefieren la reconstrucción con un autoinjerto de fémur proximal y una prótesis. También es posible usar las prótesis en silla de montar, las de tipo Mac Minn con auto o aloinjerto, las prótesis modulares y a medida, los aloinjertos masivos con o sin prótesis y las artrodesis femoroilíacas. En caso de resección del ala ilíaca y de la región acetabular, las reconstrucciones posibles son: la artrodesis isquiofemoral y femorosacra, la reconstrucción mediante autoinjerto de fémur proximal y prótesis, la medialización de la cabeza femoral, los aloinjertos y las prótesis masivas. Las amputaciones se tratan en otro artículo de la EMC.
    EMC - Aparato Locomotor. 09/2012; 45(3):1–20.
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    ABSTRACT: Delivering rhBMP-2 (recombinant Bone Morphogenic Protein-2) at low but therapeutically efficient dose is one of the current challenges for bone tissue repair. In this context, Polyelectrolyte Multilayer films (PEM) represent an attractive rhBMP-2 carrier due to their ability to protect proteins from denaturation and to coat a wide variety of materials with complex geometry. Herein, we coated macroporous TCP/HAP granules with a biopolymeric PEM film to deliver rhBMP-2 in a "matrix-bound" manner. In vitro release kinetics indicated that the PEM-coated granules sequestered significant amounts of rhBMP-2. The degree of film cross-linking influenced the quantity of rhBMP-2 trapped within the films. Bare (uncoated) TCP/HAP scaffolds were also able to retain rhBMP-2. Bioactivity of rhBMP-2 in the PEM-coated granules was confirmed on two cell markers: luciferase expression on BMP-responsive-element/Luc C2C12 cells and alkaline phosphatase activity induction on C2C12 cells. Promisingly, rhBMP-2 adsorbed onto PEM-coated and on bare granules in a lesser extent, could be stored and remained bioactive over at least 3 weeks. In vivo, both uncoated and PEM-coated TCP/HAP granules loaded with rhBMP-2 exhibited both osteoconductive and osteoinductive properties. This opens perspective for coating these bioactive PEM on other types of implantable materials, including metal alloy that do not exhibit any affinity for rhBMP-2.
    Biomaterials 10/2011; 32(30):7543-54. · 8.31 Impact Factor
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    ABSTRACT: We asked whether locally applied recombinant-Bone Morphogenic Protein-2 (rh-BMP-2) with an absorbable Type I collagen sponge (ACS) carrier could enhance the consolidation phase in a callotasis model. We performed unilateral transverse osteotomy of the tibia in 21 immature male rabbits. After a latency period of 7 days, a 3-weeks distraction was begun at a rate of 0.5mm/12h. At the end of the distraction period (Day 28) animals were randomly divided into three groups and underwent a second surgical procedure: 6 rabbits in Group I (Control group; the callus was exposed and nothing was added), 6 rabbits in Group II (ACS group; receiving the absorbable collagen sponge soaked with saline) and 9 rabbits in Group III (rh-BMP-2/ACS group; receiving the ACS soaked with 100μg/kg of rh-BMP-2, Inductos(®), Medtronic). Starting at Day 28 we assessed quantitative and qualitative radiographic parameters as well as densitometric parameters every two weeks (Days 28, 42, 56, 70 and 84). Animals were sacrificed after 8 weeks of consolidation (Day 84). Qualitative radiographic evaluation revealed hypertrophic calluses in the Group III animals. The rh-BMP-2/ACS also influenced the development of the cortex of the calluses as shown by the modified radiographic patterns in Group III when compared to Groups I and II. Densitometric analysis revealed the bone mineral content (BMC) was significantly higher in the rh-BMP-2/ACS treated animals (Group III).
    Injury 07/2011; 42(12):1460-6. · 2.46 Impact Factor
  • F Sailhan
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    ABSTRACT: Distraction osteogenesis (DO) is a surgical technique widely used in orthopedic surgery for the treatment of various pathological conditions such as leg length discrepancy, bone deformity or bone defects. The basic principle of the callotasis technique includes performing a transverse bone section before gradually distracting the two bone segments. New bone tissue is generated in the gap between the two segments. Bone regeneration during DO is believed to occur in response to the longitudinal mechanical strain applied to the callus during healing. One of the limitations of this technique is the long period of time required for the newly formed bone tissue to mineralize and consolidate. Various studies have reported that among growth factors, bone morphogenetic proteins (BMPs) may play a central role in the molecular signaling cascade leading to bone renegeration and remodeling in a DO procedure. Ongoing research is aimed at developing methods to accelerate bone consolidation in order to reduce the time required to obtain consolidation. One of these methods is to test the ability of exogenous BMPs to increase bone regeneration and accelerate bone consolidation.
    Osteoporosis International 06/2011; 22(6):2011-5. · 4.04 Impact Factor
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    ABSTRACT: The most commonly used method for unstable slipped capital femoral epiphysis (SCFE) remains in situ fixation. Depending on the surgeon's preference, screws or Kirschner wires are used for stabilizing the slipped upper femoral epiphysis. The purpose of this study was to evaluate the ability of a single cannulated screw with a proximal threading to ensure stabilization, growth, and remodeling of the slipped epiphysis. A retrospective study was performed identifying 23 children treated for unstable SCFE under 50° by means of a single cannulated screw with proximal threading. All patients attended a radiological evaluation immediately after surgery and at physeal closure. The width of the epiphysis cut by the Klein line, the width and length of the femoral neck, the centrocalcar distance (CCD) angle, and the articulotrochanteric distance (ATD) were evaluated. All patients had a stabilized epiphysis at the last follow-up, with no case of recurring slip. Radiological comparison of the affected side between the preoperative and the last follow-up evaluation showed a statistically significant improvement in the neck length and width. Fixation of the epiphysis using proximally threaded screws allows the preservation of femoral neck growth. The cannulated screw with proximal threading seems to be a safe and relevant implant to help to restore a close-to-normal hip at skeletal maturity.
    Journal of Children s Orthopaedics 04/2011; 5(2):83-8.
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    The Journal of Bone and Joint Surgery 01/2011; 93(2):195-8. · 3.23 Impact Factor
  • Digestive and Liver Disease 01/2011; 43(5):e10. · 3.16 Impact Factor
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    ABSTRACT: Los materiales osteoinductores son motivo de numerosos estudios de investigación experimental y clínica desde hace varias décadas, debido a la perspectiva terapéutica que representan en las diversas situaciones de dificultades de consolidación que suelen observarse en ortopedia. Las proteínas morfogenéticas óseas (BMP) son las únicas moléculas que hasta ahora han demostrado poseer auténtico potencial osteoinductor. Para las BMP recombinantes producidas por ingeniería genética (BMP-7 y BMP-2) existen aplicaciones clínicas restringidas (con autorizaciones de comercialización). Se presenta aquí el estado actual de la investigación sobre estas moléculas, así como los principales estudios clínicos de las BMP recombinantes disponibles en el mercado.
    EMC - Técnicas Quirúrgicas - Ortopedia y Traumatología. 01/2011; 3(2):1–17.
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    ABSTRACT: Extra-abdominal desmoid fibromatosis (EADF) is a benign tumoral condition, classically managed by more or less radical and sometimes mutilating excision. This treatment strategy is associated with a recurrence rate of nearly 50% according to various reports. EADF may show spontaneous stabilization over time. A retrospective series of 26 cases of EADF managed by simple observation was studied to assess spontaneous favorable evolution and identify possible factors impacting evolution. Eleven cases were of primary EADF with no treatment or surgery, and 15 of recurrence after surgery with no adjuvant treatment. MRI was the reference examination during follow-up. Twenty-four cases showed stabilization at a median 14 months; there were no cases of renewed evolution after stabilization. One primary tumor showed spontaneous regression, and one recurrence still showed evolution at end of follow-up (23 months). The sole factor impacting potential for evolution was prior surgery. No radiologic or pathologic criteria of evolution emerged from analysis. The present series, one of the largest dedicated to EADF managed by observation, confirmed recent literature findings: a conservative "wait-and-see" attitude is reasonable and should be considered when large-scale resection would entail significant functional or esthetic impairment. Level IV, retrospective study.
    Orthopaedics & Traumatology Surgery & Research 11/2010; 96(8):884-9. · 1.06 Impact Factor
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    ABSTRACT: Introduction Les fibromatoses desmoïdes extra-abdominales (FDEA) sont des tumeurs bénignes dont la prise en charge était habituellement fondée sur une chirurgie d’exérèse plus ou moins large, parfois mutilante, avec un taux de récidive de près de 50 % dans plusieurs séries. Hypothèse Les FDEA ont la capacité de se stabiliser spontanément avec le temps. Méthodes Les auteurs rapportent une série rétrospective de 26 cas de FDEA surveillées afin d’étudier leur évolution favorable spontanée, et d’identifier des facteurs influençant l’évolution de cette tumeur. Dans 11 cas, il s’agissait d’une FDEA primaire jamais traitée ni opérée et dans 15 cas, il s’agissait de FDEA récidivants après traitement chirurgical, les patients n’ayant bénéficié d’aucun traitement adjuvant. L’IRM était l’examen de référence pour le suivi. Résultats Dans 24 cas, les tumeurs se sont stabilisées avec une médiane de 14 mois. Après arrêt de leur évolution, aucune tumeur n’a évolué à nouveau. Une tumeur primaire a régressé spontanément et une récidive continuait à évoluer au dernier contrôle à 23 mois. Le seul facteur influençant le potentiel évolutif était la présence d’une chirurgie antérieure. Aucun critère évolutif radiologique ni anatomopathologique n’a été mis en évidence. Discussion Cette série, qui est une des plus importantes concernant des cas de fibromatoses desmoïdes extra-abdominales surveillées, confirme les données récentes de la littérature. Une attitude conservatrice wait and see est une approche raisonnable et doit être discutée quand une résection large ne peut être réalisée sans atteinte fonctionnelle ou esthétique importante. Niveau de preuve Niveau IV étude rétrospective.
    Resuscitation 01/2010; 96(8):978-984. · 4.10 Impact Factor
  • The Journal of Rheumatology 11/2009; 36(11):2621-2. · 3.26 Impact Factor

Publication Stats

150 Citations
81.32 Total Impact Points

Institutions

  • 2011–2014
    • Université René Descartes - Paris 5
      Lutetia Parisorum, Île-de-France, France
  • 2012–2013
    • Grenoble Institute of Technology
      Grenoble, Rhône-Alpes, France
    • Hôpital Cochin (Hôpitaux Universitaires Paris Centre)
      Lutetia Parisorum, Île-de-France, France
  • 2011–2013
    • Paris Diderot University
      Lutetia Parisorum, Île-de-France, France
  • 2009
    • Centre Hospitalier Universitaire de Grenoble
      Grenoble, Rhône-Alpes, France
  • 2007
    • CHU de Lyon - Hôpital Gériatrique Antoine Charial 
      Lyons, Rhône-Alpes, France