Germán Bou

Complexo Hospitalario Universitario A Coruña, La Corogne, Galicia, Spain

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Publications (172)661.5 Total impact

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    ABSTRACT: Background: Acinetobacter baumannii is a major health problem. The most common infection caused by A. baumanniiis hospital acquired pneumonia, and the associated mortality rate is approximately 50 %. Neither in vivo nor ex vivo expression profiling has been performed at the proteomic or transcriptomic level for pneumonia caused by A. baumannii. In this study, we characterized the proteome of A. baumannii under conditions that simulate those found in the airways, to gain some insight into how A. baumannii adapts to the host and to improve knowledge about the pathogenesis and virulence of this bacterium. A clinical strain of A. baumannii was grown under different conditions: in the presence of bronchoalveolar lavage fluid from infected rats, of RAW 264.7 cells to simulate conditions in the respiratory tract and in control conditions. We used iTRAQ labelling and LC-MALDI-TOF/TOF to investigate how A. baumannii responds on exposure to macrophages/BALF. Results: 179 proteins showed differential expression. In both models, proteins involved in the following proceses were over-expressed: (i) pathogenesis and virulence (OmpA, YjjK); (ii) cell wall/membrane/envelope biogenesis (MurC); (iii) energy production and conversion (acetyl-CoA hydrolase); and (iv) translation (50S ribosomal protein L9). Proteins involved in the following were under-expressed: (i) lipid metabolism (short-chain dehydrogenase); (ii) amibo acid metabolism and transport (aspartate aminotransferase); (iii) unknown function (DNA-binding protein); and (iv) inorganic ion transport and metabolism (hydroperoxidase). Conclusions: We observed alterations in cell wall synthesis and identified 2 upregulated virulence-associated proteins with >15 peptides/protein in both ex vivo models (OmpA and YjjK), suggesting that these proteins are fundamental for pathogenesis and virulence in the airways. This study is the first comprehensive overview of the ex vivo proteome of A. baumannii and is an important step towards identification of diagnostic biomarkers. Novel drug targets and potential vaccine candidates in the fight against pneumonia caused by A. baumannii.
    BMC Genomics 07/2015; 16(1). DOI:10.1186/s12864-015-1608-z · 4.04 Impact Factor
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    ABSTRACT: We examined the genetic context of 74 acquired ampC genes and 17 carbapenemase genes from 85 out of 640 Enterobacteriaceae isolates collected in 2009. Using S1-PFGE and Southern hybridization, 37 out of 74 blaAmpC genes were located on large plasmids of different sizes belonging to six Inc groups. We used sequencing and PCR mapping to investigate the regions flanking the acquired ampC genes. The blaCMY-2like genes were associated with ISEcp1, the surrounding blaDHA genes were similar to Klebsiella pneumoniae plasmid pTN60013 associated with IS26 and the psp and sap operons, and blaACC-1 genes were associated with IS26 elements inserted into ISEcp1. All the carbapenemase genes (blaVIM-1, two blaIMP-22 and blaIMP-28) were located in class 1 integrons. Therefore, although plasmids are the main cause of the rapid dissemination of ampC genes among Enterobacteriaceae, we need to be aware that other mobile genetic elements, such as insertion sequences, transposons or integrons, can be involved in the mobilization of these genes of chromosomal origin. Additionally, three new integrons are described in this study (In846 to In848). Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    Antimicrobial Agents and Chemotherapy 06/2015; DOI:10.1128/AAC.00562-15 · 4.45 Impact Factor
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    ABSTRACT: The spread of multidrug-resistant Enterobacteriaceae related to the production of extended-spectrum β-lactamases and carbapenemases is a serious public health problem worldwide. Microbiological diagnosis and therapy of these infections are challenging and controversial. Clinically relevant questions were selected and the literature was reviewed for each of them. The information from the selected articles was extracted and recommendations were provided and graded according to the strength of the recommendations and quality of the evidence. The document was opened to comments from the members from the Spanish Society of Infectious Diseases and Clinical Microbiology, which were considered for inclusion in the final version. Evidence-based recommendations are provided for the use of microbiological techniques for the detection of extended-spectrum β-lactamases and carbapenemases in Enterobacteriaceae, and for antibiotic therapy for invasive/severe infections caused by these organisms. The absence of randomised controlled trials is noteworthy; thus, recommendations are mainly based on observational studies (that have important methodological limitations), pharmacokinetic and pharmacodynamics models, and data from animal studies. Additionally, areas for future research were identified.
    Enfermedades Infecciosas y Microbiología Clínica 05/2015; DOI:10.1016/j.eimc.2014.11.009 · 1.88 Impact Factor
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    ABSTRACT: The effect of antimicrobials on the SOS-mediated mutagenesis induction depends on the bacterial species and the antimicrobial group. In this work, we studied the effect of different families of antimicrobial agents used in clinical therapy against Acinetobacter baumannii in the induction of mutagenesis in this multi-resistant gram-negative pathogen. The data showed that ciprofloxacin and tetracycline induce SOS-mediated mutagenesis, whereas colistin and meropenem, which are extensively used in clinical therapy, do not.
    Antimicrobial Agents and Chemotherapy 04/2015; 59(7). DOI:10.1128/AAC.04918-14 · 4.45 Impact Factor
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    ABSTRACT: The activity of eight aminoglycosides (amikacin, apramycin, arbekacin, gentamicin, kanamycin, neomycin, netilmicin and tobramycin) against a collection of 257 amoxicillin/clavulanic acid (AMC)-resistant Escherichia coli isolates was determined by microdilution. Aminoglycoside resistance rates, the prevalence of aminoglycoside-modifying enzyme (AME) genes, the relationship between AME gene detection and resistance phenotype to aminoglycosides, and the association of AME genes with mechanisms of AMC resistance in E. coli isolates in Spain were investigated. Aminoglycoside-resistant isolates were screened for the presence of genes encoding common AMEs [aac(3)-Ia, aac(3)-IIa, aac(3)-IVa, aac(6')-Ib, ant(2″)-Ia, ant(4')-IIa and aph(3')-Ia] or 16S rRNA methylases (armA, rmtB, rmtC and npmA). In total, 105 isolates (40.9%) were resistant to at least one of the aminoglycosides tested. Amikacin, apramycin and arbekacin showed better activity, with MIC90 values of 2mg/L (arbekacin) and 8mg/L (amikacin and apramycin). Kanamycin presented the highest MIC90 (128mg/L). The most common AME gene was aac(6')-Ib (36 strains; 34.3%), followed by aph(3')-Ia (31 strains; 29.5%), ant(2″)-Ia (29 strains; 27.6%) and aac(3)-IIa (23 strains; 21.9%). aac(3)-Ia, aac(3)-IVa, ant(4')-IIa and the four methylases were not detected. The ant(2″)-Ia gene was usually associated with OXA-1 [21/30; 70%], whilst 23/25 (92%) strains producing CTX-M-15 had the aac(6')-Ib gene. The most prevalent AME gene was aac(6')-Ib (18/41; 44%) in nosocomial isolates, whilst ant(2″)-Ia and aph(3')-Ia genes (20/64; 31%) were more frequent in strains of community origin. In 64.6% isolates the phenotypic profile correlated with the presence of commonly encountered AMEs. Copyright © 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
    International Journal of Antimicrobial Agents 04/2015; DOI:10.1016/j.ijantimicag.2015.03.008 · 4.26 Impact Factor
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    ABSTRACT: The aim of this study was to determine the impact of the carbapenemase-producing Enterobacteriaceae (CPE) in Spain in 2013 by describing their prevalence, dissemination and geographic distribution of CPE clones, their population structure and antibiotic susceptibility. From February 2013 to May 2013, 83 hospitals (about 40,000 hospital beds) prospectively collected non-duplicate Enterobacteriaceae using the screening cut-off recommended by EUCAST. Carbapenemase characterisation was performed by phenotypic methods and confirmed by PCR and sequencing. MLST types were determined for Klebsiella pneumoniae and Escherichia coli. A total of 702 Enterobacteriaceae isolates met the inclusion criteria; 379 (54%) were CPE. OXA-48 (71.5%) and VIM-1 (25.3%) were the most frequent carbapenemases, and K. pneumoniae (74.4%), Enterobacter cloacae (10.3%), and E. coli (8.4%) were the species most affected. Susceptibility to colistin, amikacin and meropenem was 95.5%, 81.3%, and 74.7%, respectively. The most prevalent STs were ST11 and ST405 in K. pneumoniae, and ST131 in E. coli. Forty-five (54.1%) of the hospitals had at least one CPE case. In K. pneumoniae, ST11/OXA-48, ST15/OXA-48, ST405/OXA-48, and ST11/VIM-1 were detected in two or more Spanish provinces. ST11 carried four carbapenemases (VIM-1, OXA-48, KPC-2, and OXA-245), but ST405 carried OXA-48 only. A wide interregional spread of CPE in Spain was observed mainly due to a few successful clones of OXA-48-producing K. pneumoniae (e.g. ST11 and ST405). Dissemination of OXA-48-producing E. coli is a new finding of public health concern. According to in vitro susceptibilities, most of the CPE (94.5%) had three or more options of antibiotic treatment. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    Antimicrobial Agents and Chemotherapy 03/2015; 59(6). DOI:10.1128/AAC.00086-15 · 4.45 Impact Factor
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    ABSTRACT: The modulating effect of N-acetylcysteine (NAC) on the activity of different antibiotics has been studied in Pseudomonas aeruginosa. Our results demonstrate that, in contrast to previous reports, only the activity of imipenem is clearly affected by NAC. Minimal inhibitory concentration and checkerboard determinations indicate that the NAC-based modulation of imipenem activity is mainly dependent on OprD. SDS-PAGE of OMPs after NAC treatments demonstrates that NAC does not modify the expression of OprD, suggesting that NAC competitively inhibits the uptake of imipenem through OprD. Similar effects on imipenem activity were obtained with P. aeruginosa clinical isolates. Our results indicate that imipenem-susceptible P. aeruginosa strains become resistant upon simultaneous treatment with NAC and imipenem. Moreover, the generality of the observed effects of NAC on antibiotic activity was assessed with two additional bacterial species, Escherichia coli and Acinetobacter baumannii. Caution should be taken during treatments as the activity of imipenem may be modified by physiologically attainable concentrations of NAC, particularly during intravenous and nebulized regimes. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    Antimicrobial Agents and Chemotherapy 03/2015; 59(6). DOI:10.1128/AAC.00017-15 · 4.45 Impact Factor
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    ABSTRACT: The spread of multidrug-resistant Enterobacteriaceae related to the production of extended-spectrum β-lactamases (ESBL) and carbapenemases is a serious public health problem worldwide. Microbiological diagnosis and therapy of these infections are challenging and controversial. After the selection of clinically relevant questions, this document provides evidence-based recommendations for the use of microbiological techniques for the detection of ESBL- and carbapenemase-producing Enterobacteriaceae, and for antibiotic therapy for invasive infections caused by these organisms. The absence of randomized-controlled trials is noteworthy, thus recommendations are mainly based on observational studies, that have important methodological limitations, pharmacokinetic and pharmacodynamics models, and data from animal studies. Additionally, areas for future research were identified. Copyright © 2014. Published by Elsevier España.
    Enfermedades Infecciosas y Microbiología Clínica 01/2015; 33(5). DOI:10.1016/j.eimc.2014.11.015 · 1.88 Impact Factor
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    ABSTRACT: Detection of carbapenemase-producing Enterobacteriaceae (CPE) is an important task at microbiology laboratories in hospitals. As the prevalence of CPE is increasing worldwide, the implementation of phenotypically based screening as well as confirmatory procedures to detect CPE are important for microbiologists. In addition to detection of carbapenem hydrolysis, the inhibition of activity against a carbapenem in the presence of several inhibitor compounds specific to class A, B, or class C beta-lactamases is a useful method to confirm the presence of carbapenemases in bacterial isolates. There is also a proteomic approach that compares the MALDI-TOF spectrum generated by the intact carbapenem (non-hydrolyzed) with that obtained after hydrolysis of the beta-lactam ring by beta-lactamase to reveal the presence of carbapenemases in bacterial isolates. Proteomic methods will probably be more frequently implemented in laboratories in the near future. Finally, molecular methods to directly or indirectly detect the presence of a carbapenemase genes are increasingly being used in microbiology laboratories. One of the main advantages of these methods is their speed, and also that they can be used directly with the clinical sample without the need for an isolated bacterial colony. Multiplex PCR, real-time PCR, DNA microarrays and pyrosequencing are some examples of molecular-based tests. Their main disadvantage is their cost, although prices are going down as the range of services increases. Surveillance of carriers is also an important task for infection control purposes. In this case, commercially available chromogenic medium supplemented with low carbapenem concentrations has shown an excellent ability to detect CPE. Moreover, molecular methods to detect specific carbapenemase genes directly from rectal swabs, stools, or other colonization sources have had excellent results.
    Enfermedades Infecciosas y Microbiología Clínica 12/2014; 32. DOI:10.1016/S0213-005X(14)70171-5 · 1.88 Impact Factor
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    ABSTRACT: Antivirulence therapy inhibits bacterial virulence factors, thus preventing the development of infection without affecting bacterial growth. The development of new antibiotics is complicated by the increasing incidence of antibiotic resistance in pathogenic bacteria. Antivirulence therapy is a promising alternative to traditional antibiotic therapy for the treatment of infectious disease, either alone or in combination with antibiotic treatment. In this review, we consider patents concerning inhibition of several bacterial virulence factors: adhesion/colonization, secretion systems, cellular signalling systems and antimicrobial resistance mechanisms. Finally, we emphasize the importance of analyzing new targets and/or molecules in this field and of considering possible resistance mechanisms
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    ABSTRACT: The US Centers for Disease Control and Prevention (CDC) recently add the advice of one-time testing of HCV infection in persons born during 1945-1965. Moreover, the US Preventive Services Task Force (USPSTF) newly recommended one-time HIV testing for persons aged 15-65. Herein, we evaluate the potential impact of these recommendations in a reference medical area of Spain. All assays results entries for HCV and HIV serological markers ordered at a reference lab from primary care and specialized physicians between 2008 and 2012 were recorded in a medical area which covers 501,526 citizens in Northern Spain. The year of birth were also documented. A total of 108,159 anti-HCV-Ab results were generated during the study period. The global rate of anti-HCV-Ab+ was 7.7% (95% CI: 7.6%-7.9%), being more prevalent in men than women (8.6% vs. 4.5%). By year of birth, the highest prevalence was found in persons born between 1955 and 1970. HCV genotype 1 was the most prevalent (59.7%) followed by genotype 3 (22.7%). Regard HIV infection, among 65,279 anti-HIV results generated the prevalence of anti-HIV+ was 1.1% (95% CI: 1.0%-1.2%), being more frequent in men (2% vs 0.5%). The years of birth with highest rates of HIV infection exactly match with those for HCV infection. The highest rates of HCV and HIV infections are found between 1960 and 1965. Different historical and social circumstances such as the huge intravenous drug use epidemic in the eighties in Spain, might explain it. Therefore, each country needs to determine its own HCV and HIV seroprevalences by year of birth to establish the proper recommendations for the screening of both infections.
    PLoS ONE 12/2014; 9(12):e113062. DOI:10.1371/journal.pone.0113062 · 3.53 Impact Factor
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    ABSTRACT: Background Treatment of multidrug-resistant Acinetobacter baumannii (MDRAB) infection presents a challenge because of the scarcity of available options. Even though combination therapy (CT) is frequently used in clinical practice, data are needed to support its use instead of monotherapy (MT).
    Journal of Antimicrobial Chemotherapy 11/2014; 69(11):3119-3126. DOI:10.1093/jac/dku233 · 5.44 Impact Factor
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    ABSTRACT: We report the complete genome sequence of Acinetobacter baumannii strain AbH12O-A2, isolated during a large outbreak in Spain. The genome has 3,875,775 bp and 3,526 coding sequences, with 39.4% G+C content. The availability of this genome will facilitate the study of the pathogenicity of the Acinetobacter species.
    Genome Announcements 11/2014; 13(2):6. DOI:10.1128/genomeA.01182-14
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    ABSTRACT: In this study MALDI-TOF MS was evaluated in the identification of anaerobic bacteria comparing it with Rapid ID 32A system. Discrepancies were solved by 16S r-RNA gene sequencing. At the species level MALDI-TOF MS identified 94.82% and Rapid ID 32A 86.67%, showing the superiority of MALDI-TOF MS to conventional methods.
    Anaerobe 09/2014; 30. DOI:10.1016/j.anaerobe.2014.09.008 · 2.36 Impact Factor
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    ABSTRACT: Acinetobacter baumannii is an extracellular opportunistic human pathogen that is becoming increasingly problematic in hospitals. In the present study, we demonstrated that the A. baumannii Omp33-36 KDa protein (Omp33-36) is a porin that acts as a channel for the passage of water. The protein is found on the cell surface and is released along with other porins in the outer membrane vesicles (OMVs). In immune and connective cell tissue, this protein induced apoptosis by activation of caspases and modulation of autophagy with the consequent accumulation of p62/ SQSTM1 (sequestosome 1) and LC3B–II (confirmed by use of autophagy inhibitors). Blockage of autophagy enables the bacterium to persist intracellularly (inside autophagosomes) with the subsequent development of cytotoxicity. Finally, we used animal models to confirm that Omp33-36 is a virulence factor in A. baumannii. Overall, the study findings show that Omp33-36 plays an important role in the pathogenesis of A. baumannii infections
    Infection and Immunity 09/2014; 82(11). DOI:10.1128/IAI.02034-14 · 4.16 Impact Factor
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    ABSTRACT: During a Spanish surveillance study, two natural variants of DHA β-lactamases, DHA-6 and DHA-7 were found, with the replacements Ala226Thr and Phe322Ser respect to DHA-1, respectively. The enzymes were isolated from Escherichia coli and Enterobacter cloacae clinical isolates, respectively. The aim of the study was the genetic, microbiological and biochemical characterization of the DHA-6 and DHA-7 β-lactamases. The blaDHA-6 andblaDHA-7 genes were located in I1 and HI2 incompatibility group plasmids of 87.3 and 310.4 kb, respectively. The gene context of both blaDHA-6 andblaDHA-7 was similar to that already described for blaDHA-1 gene and included the qnrB4 and aadA genes. The MICs for cephalothin, aztreonam, cefotaxime and ceftazidime were 8 to 30 fold lower for the DHA-6 than for DHA-1 and DHA-7 expressed in the same isogenic E.coli TG1 strain. Interestingly the MIC for cefoxitin was higher in DHA-6 expressing transformant compared to DHA-1 and DHA-7. Biochemical studies with pure β-lactamases revealed a slightly lower catalytic efficiency of DHA-6 against cephalothin, ceftazidime and cefotaxime compared to DHA-1 and DHA-7. To understand this behavior, stability experiments were carried out and showed that the DHA-6 protein displayed a significantly higher stability than DHA-1 and DHA-7 enzymes. The proximity of Thr226 to the N-terminal in the tertiary protein structure in DHA-6 may promote this stabilization and consequently could induce a slight reduction of the dynamic of this enzyme primarily affecting the hydrolysis of some of the bulkiest antibiotics.
    Antimicrobial Agents and Chemotherapy 08/2014; 58(11). DOI:10.1128/AAC.03144-14 · 4.45 Impact Factor
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    ABSTRACT: Acinetobacter baumannii is one of the most important antibiotic-resistant nosocomial bacteria. We investigated changes in the clinical and molecular epidemiology of A. baumannii over a 10-year period. We compared the data from 2 prospective multicenter cohort studies in Spain, one performed in 2000 (183 patients) and one in 2010 (246 patients), which included consecutive patients infected or colonized by A. baumannii. Molecular typing was performed by repetitive extragenic palindromic polymerase chain reaction (REP-PCR), pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST).The incidence density of A. baumannii colonization or infection increased significantly from 0.14 in 2000 to 0.52 in 2010 in medical services (p < 0.001). The number of non-nosocomial health care-associated cases increased from 1.2% to 14.2%, respectively (p < 0.001). Previous exposure to carbapenems increased in 2010 (16.9% in 2000 vs 27.3% in 2010, p = 0.03). The drugs most frequently used for definitive treatment of patients with infections were carbapenems in 2000 (45%) and colistin in 2010 (50.3%). There was molecular-typing evidence of an increase in the frequency of A. baumannii acquisition in non-intensive care unit wards in 2010 (7.6% in 2000 vs 19.2% in 2010, p = 0.01). By MSLT, the ST2 clonal group predominated and increased in 2010. This epidemic clonal group was more frequently resistant to imipenem and was associated with an increased risk of sepsis, although not with severe sepsis or mortality.Some significant changes were noted in the epidemiology of A. baumannii, which is increasingly affecting patients admitted to conventional wards and is also the cause of non-nosocomial health care-associated infections. Epidemic clones seem to combine antimicrobial resistance and the ability to spread, while maintaining their clinical virulence.
    Medicine 07/2014; 93(5):202-210. DOI:10.1097/MD.0000000000000036 · 4.87 Impact Factor
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    ABSTRACT: Bacteria that produce extended-spectrum β-lactamases (ESBLs) are an increasing health care problem and their rapid detection is a challenge that must be overcome in order to optimize antimicrobial treatment and patient care. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been used to determine resistance to β-lactams including carbapenems in Enterobacteriaceae, but methodology has not been fully validated as it remains time-consuming. We aimed to assess whether MALDI-TOF can be used to detect ESBL-producing Enterobacteriaceae from positive blood culture bottles in clinical practice. In the assay, 141 blood cultures were tested, 13 out of them were real bacteremias and 128 corresponded to blood culture bottles seeded with bacterial clinical isolates. Bacteremias were analyzed by MALDI-TOF after a positive growth result and the 128 remaining blood cultures 24 hours after the bacterial seeding. β-lactamase activity was determined through the profile of peaks associated to the antibiotics cefotaxime and ceftazidime and its hydrolyzed forms. Clavulanic acid was added to rule out the presence of non-ESBL mechanisms. Overall data show a 99% (103 out of 104) of sensitivity in detecting ESBL in positive blood cultures, respectively. Data were obtained in 90 min (maximum 150 min). Validation of the proposed methodology has a great impact in the early detection of ESBL-producing Enterobacteriaceae from positive blood cultures being a rapid and efficient method and allowing a more appropriate antibiotic therapy administration earlier. This article is protected by copyright. All rights reserved.
    Clinical Microbiology and Infection 06/2014; DOI:10.1111/1469-0691.12729 · 5.20 Impact Factor
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    ABSTRACT: Introducción: Acinetobacter baumannii es uno de los principales agentes causante de infecciones nosocomiales, produciendo brotes alrededor de todo el mundo [1]. Alarmantemente, muchos aislados clínicos son resistentes a casi todos los antibióticos de uso clínico, hecho que implica que algunas infecciones de A. baumannii sean intratables [2]. En la cepa de A. baumannii ATCC 17978, se han identificado 2 operones homólogos a umuDC (0636-7 y 1174-3) y un gen homólogo a umuD [3], todos ellos implicados en la mutagénesis inducida por daño en el DNA [4, 5]. El objetivo de este trabajo ha sido establecer la relación entre el tratamiento de este patógeno con distintos grupos de antibióticos y su capacidad de generar mutagénesis mediante la inducción de genes que codifican componentes de DNA polimerasas tendentes a error. Métodos: La inducción de los genes que codifican componentes de DNA polimerasas tendentes a error mediante el tratamiento con antibióticos se estudió a través de RT-PCR en tiempo real. Los análisis de mutagénesis se realizaron determinando la frecuencia de aparición de clones resistentes a rifampicina. Resultados: Los datos obtenidos muestran que todos los genes identificados en la cepa A. baumannii ATCC 17978 que codifican componentes de la DNA polimerasa V tendente a error (UmuDC) se inducen en presencia de varios antibióticos de uso clínico pertenecientes a los grupos de las quinolonas, los aminoglucósidos y las tetraciclinas. Sin embargo antibióticos pertenecientes a los grupos de los β-lactámicos y de las polimixinas no los inducen. Además, la inducción de estos genes se corresponde con un incremento de la tasa de mutagénesis en este patógeno. Conclusiones: La utilización de carbapenemes o colistina frente a A. baumannii no comporta el aumento de la generación de clones resistentes por mutaciones en el genoma de esta especie bacteriana.
    X Reunión Microbiología Molecular SEM, España; 06/2014
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    ABSTRACT: A carbapenem-resistant A. pittii strain carrying an OXA-24-like enzyme was isolated in Northern Spain in 2008. Sequence analysis confirmed the presence of the novel blaOXA-207 gene flanked by the site-specific XerC/XerD-like recombination binding sites and showing a unique Gly222Val substitution compared to OXA-24. Cloning and kinetic analysis showed that OXA-207 presents a reduction in the catalytic efficiency against carbapenems and a noticeably increase for oxacillin.
    Antimicrobial Agents and Chemotherapy 06/2014; 58(8). DOI:10.1128/AAC.02633-13 · 4.45 Impact Factor

Publication Stats

3k Citations
661.50 Total Impact Points


  • 2010–2015
    • Complexo Hospitalario Universitario A Coruña
      La Corogne, Galicia, Spain
  • 2005–2015
    • Complejo Hospitalario Universitario a Coruña (CHUAC)
      La Corogne, Galicia, Spain
  • 2014
    • Hospital Universitario 12 de Octubre
      Madrid, Madrid, Spain
  • 2012–2014
    • Hospital General Universitario Gregorio Marañón
      • Servicio de Microbiología
      Madrid, Madrid, Spain
    • Centro De Biología Molecular Severo Ochoa
      Madrid, Madrid, Spain
    • Hospital Universitari Son Espases
      • Department of Microbiology
      Palma, Balearic Islands, Spain
    • Université de Rouen
      Mont-Saint-Aignan, Haute-Normandie, France
  • 2003–2014
    • University of A Coruña
      La Corogne, Galicia, Spain
  • 2013
    • Instituto de Investigación Biomédica de A Coruña
      La Corogne, Galicia, Spain
  • 2010–2013
    • Hospital Universitario Marques de Valdecilla
      • Servicio de Microbiología
      Santander, Cantabria, Spain
  • 2004–2012
    • Universidad de Sevilla
      • Departamento de Microbiología
      Hispalis, Andalusia, Spain
    • Hospital Universitari de Bellvitge
      l'Hospitalet de Llobregat, Catalonia, Spain
  • 2011
    • University of Zaragoza
      • Department of Biochemistry and Molecular and Cellular Biology
      Caesaraugusta, Aragon, Spain
    • University of the Balearic Islands
      Palma, Balearic Islands, Spain
  • 2010–2011
    • Autonomous University of Barcelona
      • Department of Genetics and Microbiology
      Cerdanyola del Vallès, Catalonia, Spain
  • 2009
    • Hospital Universitario Virgen del Rocío
      • Servicio de Enfermedades Infecciosas
      Hispalis, Andalusia, Spain
    • Universidad Autónoma de Madrid
      Madrid, Madrid, Spain
  • 2008
    • Hospital do Meixoeiro
      Vigo, Galicia, Spain
  • 2007–2008
    • University Hospital Vall d'Hebron
      • Department of Infectious Diseases
      Barcino, Catalonia, Spain
  • 2004–2006
    • Hospital Clínic de Barcelona
      • Servicio de Microbiología
      Barcelona, Catalonia, Spain
  • 1999–2006
    • Hospital Universitario Ramón y Cajal
      • Departamento de Microbiologia y Parasitología
      Madrid, Madrid, Spain
  • 2001
    • Mayo Clinic - Rochester
      Rochester, Minnesota, United States
    • Mayo Foundation for Medical Education and Research
      • Division of Infectious Diseases
      Scottsdale, AZ, United States