Fei-Yun Lo

Publications (2)3.99 Total impact

• Source

  Available from: PubMed Central

  Article: EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis.

  Ting-Yun Liu, Shang-Ju Wu, Mi-Hsin Huang, Fei-Yun Lo, Mong-Hsun Tsai, Ching-Hwa Tsai, Su-Ming Hsu, Chung-Wu Lin
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  ABSTRACT: About 30-50% of Hodgkin lymphomas (HLs) harbor the Epstein-Barr virus (EBV), but the impact of EBV infection on clinical outcomes has been unclear. EBV-encoded small RNAs (EBERs) are presented in all EBV-infected cells, but their functions are still less understood. EBER1 was transfected into two HL cell lines, KMH2 and L428, and microarrays were used to screen for EBER1-induced changes. We found that EBER1 suppressed p21cip1/waf1 transcription in HL cell lines. In addition, positive regulators of p21cip1/waf1 transcription, such as p53, EGR1, and STAT1, were decreased. Suppression of p21cip1/waf1 in the EBER1+ HL cell lines was associated with increased resistance to histone deacetylase inhibitors or proteasome inhibitors, drugs known to cause apoptosis by increasing p21cip1/waf1 levels. On biopsy specimens, EBV+ HLs had weaker expression of both p21cip1/waf1 and active caspase 3. Clinically, suppression of p21cip1/waf1 in EBV+ HLs was associated with a worse 2-year disease-free survival rate (45% for EBV+ HLs vs. 77% for EBV- HLs, p = 0.002). Although the underlying mechanisms are still relatively unclear, EBER1 inhibits p21cip1/waf1 transcription and prevents apoptosis through down-regulation of p53, EGR1, and STAT1. The anti-apoptotic activity of EBER1 may be important in the rescue of Reed-Sternberg cells from drug-induced apoptosis and in the clinical behaviors of EBV+ HLs.
  Molecular Cancer 02/2010; 9:32. · 3.99 Impact Factor
• Article: EBV-positive Hodgkin lymphoma is associated with suppression of p21^cip1/waf1and a worse prognosis

  Ting-Yun Liu, Shang-Ju Wu, Mi-Hsin Huang, Fei-Yun Lo, Mong-Hsun Tsai, Ching-Hwa Tsai, Su-Ming Hsu, Chung-Wu Lin
  [show abstract] [hide abstract]
  ABSTRACT: Abstract Background About 30-50% of Hodgkin lymphomas (HLs) harbor the Epstein-Barr virus (EBV), but the impact of EBV infection on clinical outcomes has been unclear. EBV-encoded small RNAs ( EBER s) are presented in all EBV-infected cells, but their functions are still less understood. Results EBER1 was transfected into two HL cell lines, KMH2 and L428, and microarrays were used to screen for EBER1 -induced changes. We found that EBER1 suppressed p21 ^cip1/waf1transcription in HL cell lines. In addition, positive regulators of p21 ^cip1/waf1transcription, such as p53, EGR1, and STAT1, were decreased. Suppression of p21 ^cip1/waf1in the EBER1 ⁺HL cell lines was associated with increased resistance to histone deacetylase inhibitors or proteasome inhibitors, drugs known to cause apoptosis by increasing p21^cip1/waf1levels. On biopsy specimens, EBV⁺HLs had weaker expression of both p21^cip1/waf1and active caspase 3. Clinically, suppression of p21^cip1/waf1in EBV⁺HLs was associated with a worse 2-year disease-free survival rate (45% for EBV⁺HLs vs . 77% for EBV^-HLs, p = 0.002). Conclusion Although the underlying mechanisms are still relatively unclear, EBER1 inhibits p21 ^cip1/waf1transcription and prevents apoptosis through down-regulation of p53, EGR1, and STAT1. The anti-apoptotic activity of EBER1 may be important in the rescue of Reed-Sternberg cells from drug-induced apoptosis and in the clinical behaviors of EBV⁺HLs.
  Molecular Cancer. 01/2010;