Entao Wang

Instituto Politécnico Nacional, Ciudad de México, Mexico City, Mexico

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Publications (13)33.71 Total impact

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    ABSTRACT: In present study, we report our extensive survey on the diversity and biogeography of rhizobia associated with Sophora flavescens, a sophocarpidine (matrine)-containing medicinal legume. We additionally investigated the cross nodulation, infection pattern, light and electron microscopies of root nodule sections of S. flavescens infected by various rhizobia. Seventeen genospecies of rhizobia belonging to 5 genera with 7 types of symbiotic nodC genes were found to nodulate S. flavescens in natural soils. In the cross nodulation tests, most representative rhizobia in the class alpha-Proteobacteria whose host plants belong to different cross-nodulation groups form effective indeterminate nodules, while representative rhizobia in the class beta-Proteobacteria form ineffective nodules on S. flavescens. Highly host-specific biovars of Rhizobium leguminosarum (bv. trifolii and bv. viciae) and R. etli bv. phaseoli could establish symbioses with S. flavescens, providing further evidence that S. flavescens is an extremely promiscuous legume and it does not have strict selectivity on either the symbiotic genes or the species-determining housekeeping genes of rhizobia. Root hair infection is found as the pattern that rhizobia have gained entry into the curled root hairs. Electron microscopies of ultra-thin sections of S. flavescens root nodules formed by different rhizobia show that the bacteroids are regular or irregular rod shape and nonswollen types. Some bacteroids contain poly-β-hydroxybutyrate (PHB) while others do not, indicating the synthesis of PHB in bacteroids is rhizobia-dependent. The extremely promiscuous symbiosis between S. flavescens and different rhizobia provide us a basis for future studies aimed at understanding the molecular interactions of rhizobia and legumes.
    Molecular Plant-Microbe Interactions 09/2015; DOI:10.1094/MPMI-06-15-0141-R · 3.94 Impact Factor
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    ABSTRACT: The aim of this study was to learn the interactions among the endophytic bacteria, the plant growth, the foliar spray of fulvic acid, and the accumulation of steviol glycosides in the leaves of Stevia rebaudiana. Metagenomic DNA was extracted from the Stevia leaves at different growth stages with or without the fulvic acid treatment; and the diversity of endophytic bacteria in Stevia leaves was estimated by pyrosequencing of 16S rRNA genes. As results, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes were found to be the dominant phyla despite the growth stages and fulvic acid application. Stevia growth stages strongly regulated composition of endophytic community. The genera Agrobacterium (12.3%) and Erwinia (7.2%) dominated in seedling stage were apparently declined in the vegetable and initial flowering stages, while Sphingomonas and Methylobacterium increased in mature leaves at harvest time, which showed that the mature leaves of Stevia preferred to accumulate some certain endophytic bacteria. Sphingomonas and Methylobacterium constituted an important part of the core endophytic community and were positively correlated with the stevioside content and UGT74G1 gene expression, respectively; while Erwinia, Agrobacterium, and Bacillus were negatively correlated with the stevioside accumulation. Fulvic acid treatment accelerated the variation of endophytes along the growth stages and increased the steviol glycosides content. This is the first study to reveal the community composition of endophytic bacteria in the Stevia leaves, to evidence the strong effects of growth stage and fulvic acid application on the endophytes of Stevia, and to demonstrate the correlation between the endophytic bacteria and the steviol glycosides accumulation.
    Frontiers in Microbiology 08/2015; 6. DOI:10.3389/fmicb.2015.00867 · 3.99 Impact Factor
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    ABSTRACT: Although the biogeography of rhizobia has been investigated extensively, little is known about the adaptive molecular evolution of rhizobia influenced by soil environments and selected by legumes. In this study, microevolution of Mesorhizobium strains nodulating Caragana in a semi-fixing desert belt in northern China was investigated. Five core genes-atpD, glnII, gyrB, recA, and rpoB, six heat-shock factor genes-clpA, clpB, dnaK, dnaJ, grpE, and hlsU, and five nodulation genes-nodA, nodC, nodD, nodG, and nodP, of 72 representative mesorhizobia were studied in order to determine their genetic variations. A total of 21 genospecies were defined based on the average nucleotide identity (ANI) of concatenated core genes using a threshold of 96% similarity, and by the phylogenetic analyses of the core/heat-shock factor genes. Significant genetic divergence was observed among the genospecies in the semi-fixing desert belt (areas A-E) and Yunnan province (area F), which was closely related to the environmental conditions and geographic distance. Gene flow occurred more frequently among the genospecies in areas A-E, and three sites in area B, than between area F and the other five areas. Recombination occurred among strains more frequently for heat-shock factor genes than the other genes. The results conclusively showed that the Caragana-associated mesorhizobia had divergently evolved according to their geographic distribution, and have been selected not only by the environmental conditions but also by the host plants. Copyright © 2015 Elsevier GmbH. All rights reserved.
    Systematic and Applied Microbiology 03/2015; 38(3). DOI:10.1016/j.syapm.2015.02.007 · 3.28 Impact Factor
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    ABSTRACT: Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73mgL(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Bioresource Technology 01/2015; 182C:18-25. DOI:10.1016/j.biortech.2015.01.100 · 4.49 Impact Factor
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    ABSTRACT: The legume species of Astragalus as traditional Chinese medicine source and environmental protection plants showed an extensive distribution in the arid region of northwestern China. However, few rhizobia associating with Astragalus have been investigated in this region so far. In this study, 78 endophytic bacteria were isolated from root nodules of 12 Astragalus species and characterized by the PCR-RFLP of 16S rRNA gene and symbiotic genes together with the phylogenetic analysis. Results showed that the majority (53%) of isolates are non-nodulating Agrobacterium sp. and the rest are Mesorhizobium genomic species (41%), Ensifer spp. and Rhizobium gallicum (6%), respectively. Mesorhizobium genomic species are broadly distributed in the Astragalus symbioses and most of them share similar symbiotic genes. It seems that horizontal gene transfer occurred frequently among different genomic species independent of their original hosts and sites. Astragalus adsurgens is nodulated by a widely range of rhizobial species in the nodulation test, revealing that it could play an important role in diversification of Astragalus symbionts and that might be a reason for its wide adaptation to diverse environments.
    Journal of Basic Microbiology 01/2015; 55(1). DOI:10.1002/jobm.201300209 · 1.82 Impact Factor
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    ABSTRACT: Two novel Gram-negative strains (CCBAU 03422T and CCBAU 03415) isolated from root nodules of Sophora flavescens were phylogenetically classified into the genus of Phyllobacterium based on the comparative analysis of 16S rRNA and atpD genes. They showed 99.8% rRNA gene sequence similarities to Phyllobacterium brassicacearum LMG 22836T, and CCBAU 03422T showed 91.2% and 88.6% atpD gene similarities to strains P. endophyticum LMG 26470T and P. brassicacearum LMG 22836T, respectively. Strain CCBAU 03422T contained Q-10 as its major quinone and showed cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of the related species. DNA-DNA relatedness (lower than 48.8%) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422T could nodulate and fix nitrogen effectively on its original host plant, Sophora flavescens. Based upon the studies mentioned above, a novel species named as Phyllobacterium sophorae is proposed and the type strain is CCBAU 03422T (= A-6-3T = LMG 27899T = HAMBI 3508T).
    International Journal of Systematic and Evolutionary Microbiology 11/2014; 65(Pt 2). DOI:10.1099/ijs.0.067017-0 · 2.51 Impact Factor
  • Jie Liu · Entao Wang · Wenxin Chen
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    ABSTRACT: Recently, some research reports showed varied endophytic bacteria in the root nodules of some legumes, which attracts great interest in research field of rhizobia. Here, we reviewed the discovery, identification of some endophytic bacteria (Agrobacterium, non-symbiotic rhizobia, and other bacteria) in root nodules and their influence on symbiosis or plant growth, to understand the microecosystem of root nodule and to extend the field of rhizobia research.
    ACTA MICROBIOLOGICA SINICA 08/2011; 51(8):1001-6.
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    ABSTRACT: A total of seventy-five symbiotic bacterial strains isolated from root nodules of wild Sophora alopecuroides grown in different regions of China's Loess Plateau were characterized. Based on the combined RFLP patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including Mesorhizobium alhagi and M. gobiense as the main groups, as well as Agrobacterium tumefaciens, M. amorphae, Phyllobacterium trifolii, Rhizobium giardinii, R. indigoferae, Sinorhizobium fredii and S. meliloti as the minor groups according to the 16S rRNA and recA gene analyses. Five and three lineages of nodA and nifH were found, respectively, in these strains, implying that the symbiotic genes of the S. alopecuroides rhizobia had different origins or had divergently evolved. Results of correspondence analysis showed that there was a correlation between rhizobial genotypes and the geographic origins. Possible lateral transfer of the recA and 16S rRNA genes between the P. trifolii and A. tumefaciens strains, and that of symbiotic genes (nodA, nifH) between different genera, was shown by discrepancies of the phylogenetic relationships of the four gene loci. These results revealed diverse rhizobia associated with wild S. alopecuroides grown in different regions of China's Loess Plateau, and demonstrated for the first time the existence of symbiotic A. tumefaciens strains in root nodules of S. alopecuroides.
    Systematic and Applied Microbiology 10/2010; 33(8):468-77. DOI:10.1016/j.syapm.2010.08.004 · 3.28 Impact Factor
  • XinHua Sui · LiLi Han · EnTao Wang · Feng Jiang · YiHai Liu · WenXin Chen
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    ABSTRACT: Fifty rhizobial isolates of Lathyrus and Oxytropis collected from northern regions of China were studied in their genotypic characterization based upon analyses of ARDRA, 16S-23S IGS PCR-RFLP, TP-RAPD, MLEE, sequences of 16S rDNA gene and housekeeping genes of atpD, recA and glnII. The results demonstrated that most of the Lathyrus rhizobia belonged to Rhizobium and most of the Oxytropis rhizobia belonged to Sinorhizobium. A novel group of Rhizobium sp. I and S. meliloti were identified as the main microsymbionts respectively associated with Lathyrus and Oxytropis species in the collection area, which were new associations between rhizobia and the mentioned hosts. This study also provides new evidence for biogeography of rhizobia.
    Science in China Series C Life Sciences 03/2009; 52(2):182-92. DOI:10.1007/s11427-008-0132-7 · 1.61 Impact Factor
  • Zhiwei Lv · Jinshui Yang · Entao Wang · Hongli Yuan
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    ABSTRACT: Sugarcane bagasse (SCB) is a residual product of sugar processing, containing mainly lignocellulose. The biodegradation of cellulose is an economical approach to recycling SCB. To obtain an effective microbial community and study the SCB biodegradation process, a SCB-degrading mesophilic microbial community, EMSD13, growing at 50 °C was isolated. More than 77% of alkali pretreated SCB was degraded and over 83% of the cellulose it contained was utilized by the EMSD13 community within 6 days. To understand the biodegradation procedure of EMSD13, extracellular and SCB-absorbable cellulases were analyzed. The peak activity (42 mU/mL) of the extracellular cellulase in the supernatant occurred on the 3rd day of incubation. About 73.6 mg of substrate-bound proteins with a cellulase activity of 61.1 mU/mg were recovered from residual SCB by eluting with 3 M guanidine hydrochloride. Also, 15.9 mg of proteins with 28.6 mU/mg of cellulase activity were obtained from residual SCB by eluting with 6 M urea. SDS-PAGE analysis showed that cellulases in both elutes were similar, but most differed from those in the supernatant. This is the first report on substrate-bound cellulases from a microbial community.
    PROCESS BIOCHEMISTRY 12/2008; 43(12-43):1467-1472. DOI:10.1016/j.procbio.2008.08.001 · 2.52 Impact Factor
  • HonGli Yuan · ZhiJian Li · JiaoYan Ying · EnTao Wang
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    ABSTRACT: A cadmium(II)-resistant fungus, strain F2, isolated from blende soil was identified as Phoma sp. by morphological study and internal transcribed spacer sequencing. This strain could accumulate 280 mg of Cd(II)/g dry weight mycelium. In liquid medium containing 163.8 mg Cd(II)/L, 96% of Cd(II) was removed by the actively growing mycelium. In addition, both oven-dried and lyophilized mycelium could effectively adsorb Cd(II). There were removed 91% and 46.2% of Cd(II) from 51.6 mg Cd(II)/L solution by lyophilized biomass and oven-dried biomass respectively. Transmission electron microscopy and energy-dispersive X-ray analysis showed the accumulation of Cd(II) in the mycelium cell walls. Our results demonstrated that Phoma sp. F2 was a hyperaccumulator for the removal of Cd(II) from contaminated soil and water.
    Current Microbiology 10/2007; 55(3):223-7. DOI:10.1007/s00284-007-0088-z · 1.42 Impact Factor
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    ABSTRACT: TfxG, one of the tfxABCDEFG cluster genes that code for trifolitoxin (TFX) production, was initially described in Rhizobium leguminosarum bv. trifolii T24. Although several genes in the tfx family have functions related to TFX production or resistance to TFX, the function of tfxG is largely unknown. Using cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis, we found that expression of the tfxG gene dramatically increased under alkaline culture conditions in Sinorhizobium meliloti CCBAU 81024. This result was confirmed by northern blot analysis. Mutagenesis of tfxG significantly decreased the viability of Sinorhizobium meliloti CCBAU 81024 under alkali stress. Complementation of the tfxG mutant strain using the functional tfxG gene recovered its alkali tolerance to a wild-type level. Genomic analysis of the tfxG gene suggests that choline and homoserine kinase domains may contribute to its alkali tolerance function. This is the first clear evidence that tfxG plays a crucial role in the alkali tolerance of S. meliloti CCBAU 81024, and the finding provides its biological function.
    Research in Microbiology 07/2007; 158(6):501-5. DOI:10.1016/j.resmic.2007.04.003 · 2.71 Impact Factor
  • Jin Shui Yang · Jin Ren Ni · Hong Li Yuan · EnTao Wang
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    ABSTRACT: Biodegradation of wood chips of broad-leaved trees Eucalyptus grandis×Eucalyptus urophylla and Populus canadensis, and of the conifer Larix olgensis by Pseudomonas sp. PKE117 was studied. After 60 days of biopulping by PKE117, the weight losses of E. grandis×E. urophylla, P. canadensis and L. olgensis were 7.58%, 17.15% and 26.75%, respectively. Elemental analysis showed that the C, H contents of three wood chips decreased and O content increased after the biopulping. FT-IR results also showed that the lignin structure was destroyed more than that of cellulose. Solid-state CP/MAS 13C-NMR spectroscopy results showed that the content of guaiacylpropanoid (G) lignin-unit in lignin of three samples decreased after biodegradation. The hemicellulose in L. Olgensis decreased much more than other samples. All the results demonstrated that the degradation of L. olgensis by Pseudomonas sp. PKE117 was more efficient than that of P. canadensis and E. grandis×E. urophylla. The strain Pseudomonas sp. PKE117 could be an alternative bioagent to replace the white-rot fungi in the biopulping of softwood.
    International Biodeterioration & Biodegradation 07/2007; 60(2):90-95. DOI:10.1016/j.ibiod.2006.12.006 · 2.13 Impact Factor

Publication Stats

45 Citations
33.71 Total Impact Points


  • 2007–2015
    • Instituto Politécnico Nacional
      • Departamento de Microbiología
      Ciudad de México, Mexico City, Mexico
  • 2011
    • Qingdao University of Science and Technology
      Tsingtao, Shandong Sheng, China
  • 2009
    • China Agricultural University
      • State Key Laboratory for Agrobiotechnology
      Peping, Beijing, China