Entao Wang

Qingdao University of Science and Technology, Tsingtao, Shandong Sheng, China

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Publications (6)6.02 Total impact

  • Jie Liu, Entao Wang, Wenxin Chen
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    ABSTRACT: Recently, some research reports showed varied endophytic bacteria in the root nodules of some legumes, which attracts great interest in research field of rhizobia. Here, we reviewed the discovery, identification of some endophytic bacteria (Agrobacterium, non-symbiotic rhizobia, and other bacteria) in root nodules and their influence on symbiosis or plant growth, to understand the microecosystem of root nodule and to extend the field of rhizobia research.
    ACTA MICROBIOLOGICA SINICA 08/2011; 51(8):1001-6.
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    ABSTRACT: Fifty rhizobial isolates of Lathyrus and Oxytropis collected from northern regions of China were studied in their genotypic characterization based upon analyses of ARDRA, 16S-23S IGS PCR-RFLP, TP-RAPD, MLEE, sequences of 16S rDNA gene and housekeeping genes of atpD, recA and glnII. The results demonstrated that most of the Lathyrus rhizobia belonged to Rhizobium and most of the Oxytropis rhizobia belonged to Sinorhizobium. A novel group of Rhizobium sp. I and S. meliloti were identified as the main microsymbionts respectively associated with Lathyrus and Oxytropis species in the collection area, which were new associations between rhizobia and the mentioned hosts. This study also provides new evidence for biogeography of rhizobia.
    Science in China Series C Life Sciences 03/2009; 52(2):182-92. · 1.61 Impact Factor
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    ABSTRACT: Sugarcane bagasse (SCB) is a residual product of sugar processing, containing mainly lignocellulose. The biodegradation of cellulose is an economical approach to recycling SCB. To obtain an effective microbial community and study the SCB biodegradation process, a SCB-degrading mesophilic microbial community, EMSD13, growing at 50 °C was isolated. More than 77% of alkali pretreated SCB was degraded and over 83% of the cellulose it contained was utilized by the EMSD13 community within 6 days. To understand the biodegradation procedure of EMSD13, extracellular and SCB-absorbable cellulases were analyzed. The peak activity (42 mU/mL) of the extracellular cellulase in the supernatant occurred on the 3rd day of incubation. About 73.6 mg of substrate-bound proteins with a cellulase activity of 61.1 mU/mg were recovered from residual SCB by eluting with 3 M guanidine hydrochloride. Also, 15.9 mg of proteins with 28.6 mU/mg of cellulase activity were obtained from residual SCB by eluting with 6 M urea. SDS-PAGE analysis showed that cellulases in both elutes were similar, but most differed from those in the supernatant. This is the first report on substrate-bound cellulases from a microbial community.
    Process Biochemistry. 01/2008;
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    ABSTRACT: A cadmium(II)-resistant fungus, strain F2, isolated from blende soil was identified as Phoma sp. by morphological study and internal transcribed spacer sequencing. This strain could accumulate 280 mg of Cd(II)/g dry weight mycelium. In liquid medium containing 163.8 mg Cd(II)/L, 96% of Cd(II) was removed by the actively growing mycelium. In addition, both oven-dried and lyophilized mycelium could effectively adsorb Cd(II). There were removed 91% and 46.2% of Cd(II) from 51.6 mg Cd(II)/L solution by lyophilized biomass and oven-dried biomass respectively. Transmission electron microscopy and energy-dispersive X-ray analysis showed the accumulation of Cd(II) in the mycelium cell walls. Our results demonstrated that Phoma sp. F2 was a hyperaccumulator for the removal of Cd(II) from contaminated soil and water.
    Current Microbiology 10/2007; 55(3):223-7. · 1.52 Impact Factor
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    ABSTRACT: TfxG, one of the tfxABCDEFG cluster genes that code for trifolitoxin (TFX) production, was initially described in Rhizobium leguminosarum bv. trifolii T24. Although several genes in the tfx family have functions related to TFX production or resistance to TFX, the function of tfxG is largely unknown. Using cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis, we found that expression of the tfxG gene dramatically increased under alkaline culture conditions in Sinorhizobium meliloti CCBAU 81024. This result was confirmed by northern blot analysis. Mutagenesis of tfxG significantly decreased the viability of Sinorhizobium meliloti CCBAU 81024 under alkali stress. Complementation of the tfxG mutant strain using the functional tfxG gene recovered its alkali tolerance to a wild-type level. Genomic analysis of the tfxG gene suggests that choline and homoserine kinase domains may contribute to its alkali tolerance function. This is the first clear evidence that tfxG plays a crucial role in the alkali tolerance of S. meliloti CCBAU 81024, and the finding provides its biological function.
    Research in Microbiology 01/2007; 158(6):501-5. · 2.89 Impact Factor
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    ABSTRACT: Biodegradation of wood chips of broad-leaved trees Eucalyptus grandis×Eucalyptus urophylla and Populus canadensis, and of the conifer Larix olgensis by Pseudomonas sp. PKE117 was studied. After 60 days of biopulping by PKE117, the weight losses of E. grandis×E. urophylla, P. canadensis and L. olgensis were 7.58%, 17.15% and 26.75%, respectively. Elemental analysis showed that the C, H contents of three wood chips decreased and O content increased after the biopulping. FT-IR results also showed that the lignin structure was destroyed more than that of cellulose. Solid-state CP/MAS 13C-NMR spectroscopy results showed that the content of guaiacylpropanoid (G) lignin-unit in lignin of three samples decreased after biodegradation. The hemicellulose in L. Olgensis decreased much more than other samples. All the results demonstrated that the degradation of L. olgensis by Pseudomonas sp. PKE117 was more efficient than that of P. canadensis and E. grandis×E. urophylla. The strain Pseudomonas sp. PKE117 could be an alternative bioagent to replace the white-rot fungi in the biopulping of softwood.
    International Biodeterioration & Biodegradation - INT BIODETERIOR BIODEGRAD. 01/2007; 60(2):90-95.

Publication Stats

20 Citations
6.02 Total Impact Points

Institutions

  • 2011
    • Qingdao University of Science and Technology
      Tsingtao, Shandong Sheng, China
  • 2007–2009
    • China Agricultural University
      • College of Biological Sciences
      Beijing, Beijing Shi, China
    • National Polytechnic Institute
      • Departamento de Microbiología
      Villa Gustavo A. Madero, The Federal District, Mexico