E. Spalburg

Publications of E. Spalburg

• Molecular characterisation of PFGE non-typable methicillin-resistant Staphylococcus aureus in The Netherlands, 2007.

  Authors: X.W. Huijsdens, T Bosch, M G van Santen-Verheuvel, E. Spalburg, G N Pluister, M van Luit, M E O C Heck, A Haenen, A J de Neeling

  Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin. 01/2009; 14(38).

  In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed byIn 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA isolates revealed 27 different spa types and two distinct SCCmec types, type IV and V. All NT-MRSA isolates were closely related based on spa and multi-locus sequence typing and belonged to the ST398 lineage. The rapid increase of NT-MRSA (ST398) isolates over the last years shows the importance of this relatively new clonal lineage.

• Multiple cases of familial transmission of community-acquired methicillin-resistant Staphylococcus aureus.

  Authors: X.W. Huijsdens, M G van Santen-Verheuvel, E. Spalburg, M E O C Heck, G N Pluister, B A Eijkelkamp, A J de Neeling, W J B Wannet

  Journal of clinical microbiology. 09/2006; 44(8):2994-6.

  The worldwide emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can have severe public health implications. Familial transmissions of CA-MRSA in The NetherlandsThe worldwide emergence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can have severe public health implications. Familial transmissions of CA-MRSA in The Netherlands were investigated. Among the families studied, two clusters of CA-MRSA could be identified. This report demonstrates that family members can serve as reservoirs of CA-MRSA which may become a serious problem in containing the spread of MRSA.

• High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

  Authors: M Aires de Sousa, K Boye, H de Lencastre, A Deplano, M C Enright, J Etienne, A Friedrich, D Harmsen, A Holmes, X.W. Huijsdens [......] J K Rasheed, E. Spalburg, B Strommenger, M J Struelens, F C Tenover, J Thomas, U Vogel, H Westh, J Xu, W Witte

  Journal of clinical microbiology. 03/2006; 44(2):619-21.

  Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureusCurrent DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

• High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

  Authors: M. ires-de-Sousa, K. Boye, Lencastre H. de, A. Deplano, M.C. Enright, J. Etienne, A. Friedrich, D. Harmsen, A. Holmes, X.W. Huijsdens [......] J.K. Rasheed, E. Spalburg, B. Strommenger, M.J. Struelens, F.C. Tenover, J. Thomas, U. Vogel, H. Westh, J. Xu, W. Witte

  J.Clin.Microbiol. 02/2006; 44.

  Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureusCurrent DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature

• Emergence of virulent methicillin-resistant Staphylococcus aureus strains carrying Panton-Valentine leucocidin genes in The Netherlands.

  Authors: W J B Wannet, E. Spalburg, M E O C Heck, G N Pluister, E Tiemersma, R.J.L. Willems, X.W. Huijsdens, A J de Neeling, J Etienne

  Journal of clinical microbiology. 08/2005; 43(7):3341-5.

  Methicillin-resistant Staphylococcus aureus (MRSA) strains carrying the Panton-Valentine leucocidin (PVL) genes have been reported worldwide and are a serious threat to public health. The PVL genesMethicillin-resistant Staphylococcus aureus (MRSA) strains carrying the Panton-Valentine leucocidin (PVL) genes have been reported worldwide and are a serious threat to public health. The PVL genes encode a highly potent toxin which is involved in severe skin infections and necrotizing pneumonia, even in previously healthy individuals. We assessed the prevalence of PVL-positive MRSA in The Netherlands for two periods of time: (i) 1987 through 1995 and (ii) 2000 and 2002, and determined their characteristics by using multilocus sequence typing and staphylococcal chromosome cassette (SCCmec) typing. It was found that up to 15% of all MRSA isolates detected in The Netherlands harbored the PVL genes. Most PVL-positive MRSA isolates were obtained from severe soft tissue infections in relatively young individuals. The first PVL-positive MRSA described in The Netherlands, isolated in 1988, was a single-locus variant of the "Berlin" epidemic MRSA clone. The 20 PVL-positive MRSA isolates studied in 2000 and 2002 consisted of five different sequence types (STs) that belonged to four clonal complexes. One of the STs, ST80, is considered to be a widespread European clone and was the most predominant ST (60%) in this study, while ST37 had never been found to be associated with PVL-positive MRSA. Most isolates harbored SCCmec type IV, a supposed marker for community-acquired MRSA. The number and type of virulence-associated genes varied among the different STs.

• Panton-Valentine leukocidin positive MRSA in 2003: the Dutch situation.

  Authors: W J B Wannet, M E O C Heck, G N Pluister, E. Spalburg, M G van Santen, X W Huijsdans, E Tiemersma, A J de Neeling

  Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin. 12/2004; 9(11):28-9.

  Analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolates in the Netherlands in 2003 revealed that 8% of the hospital isolates carried the loci for Panton-Valentine leukocidin (PVL).Analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolates in the Netherlands in 2003 revealed that 8% of the hospital isolates carried the loci for Panton-Valentine leukocidin (PVL). Molecular subtyping showed that most Dutch PVL-MRSA genotypes corresponded to well-documented global epidemic types. The most common PVL-MRSA genotypes were sequence type ST8, ST22, ST30, ST59 and ST80. MRSA with ST8 increased in the Netherlands from 1% in 2002 to 17% in 2003. It is emphasised that PVL-MRSA might not only emerge in the community, but also in the hospital environment.

• Widespread dissemination in The Netherlands of the epidemic berlin methicillin-resistant Staphylococcus aureus clone with low-level resistance to oxacillin.

  Authors: W J B Wannet, E. Spalburg, M E O C Heck, G N Pluister, R.J.L. Willems, A J de Neeling

  Journal of clinical microbiology. 08/2004; 42(7):3077-82.

  Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen and represents a growing public health burden due to the emergence and spread of epidemic strains, particularlyMethicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen and represents a growing public health burden due to the emergence and spread of epidemic strains, particularly within the hospital environment. An epidemic MRSA clone, with characteristic low-level resistance to oxacillin, emerged in the year 2000 and became endemic in the Netherlands. Multilocus sequence typing characterized the strain as sequence type 45, which was previously designated the Berlin epidemic MRSA clone. In 2 years, this strain has become the predominant MRSA clone in the Netherlands.

• Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in and humans pigs

  Authors: A van Belkum, D.C. Melles, J.K. Peeters, W.B. van Leeuwen, E. van Duijkeren, X.W. Huijsdens, E. Spalburg, A J de Neeling, H A Verbrugh

  Methicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers. ST398 methicillin-susceptible S. aureus circulates among humans at lowMethicillin-resistant Staphylococcus aureus sequence type 398 (ST398 MRSA) was identified in Dutch pigs and pig farmers. ST398 methicillin-susceptible S. aureus circulates among humans at low frequency (0.2%) but was isolated in 3 human cases of bacteremia (2.1%; p = 0.026). Although its natural host is probably porcine, ST398 MRSA likely causes infections in humans.

• High prevalence of methicillin resistant Staphylococcus aureus in pigs

  Authors: A J de Neeling, M.J.M. van den Broek, E. Spalburg, M G van Santen-Verheuvel, W.D.C. Dam-Deisz, H.C. Boshuizen, A. van der Giessen, E. van Duijkeren, X.W. Huijsdens

• Transmission of methicillin-resistant Staphylococcus aureus strains between different kinds of pig farms

  Authors: E. van Duijkeren, R. Ikawaty, M.J. Broekhuizen-Stins, M.D. Jansen, E. Spalburg, A J de Neeling, JG Allaart, A. van Nes, J.A. Wagenaar, A.C. Fluit

• Widespread dissemination in The Netherlands of the epidemic berlin methicillin-resistant Staphylococcus aureus clone with low-level resistance to oxacillin

  Authors: W J B Wannet, E. Spalburg, M.E. Heck, G N Pluister, R.J.L. Willems, A J de Neeling

• Epidemic and nonepidemic multidrug-resistant Enterococcus faecium

  Authors: H.L. Laevis, R.J.L. Willems, J. Top, E. Spalburg, E.M. Mascini, A.C. Fluit, I.M. Hoepelman, A J de Neeling, M.J.M. Bonten

• Emergence of virulent methicillin-resistant Staphylococcus aureus strains carrying Panton-Valentine leucocidin genes in The Netherlands

  Authors: W J B Wannet, E. Spalburg, M.E. Heck, G N Pluister, E Tiemersma, R.J.L. Willems, X.W. Huijsdens, A J de Neeling, J Etienne