Dong Wook Shin

Seoul National University Hospital, Sŏul, Seoul, South Korea

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Publications (132)367.69 Total impact

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    ABSTRACT: Testosterone is an endocrine hormone with functions in the reproductive organs, anabolic events and skin homeostasis. We report here that GPRC6A serves as a sensor and mediator of the rapid action of testosterone in epidermal keratinocytes. The silencing of GPRC6A inhibited testosterone-induced intracellular calcium ([Ca2+]i) mobilization and H2O2 generation. These results indicated that a testosterone-GPRC6A complex is required for the activation of Gq protein, IP3 generation, and [Ca2+]i mobilization, leading to Duox1 activation. H2O2 generation by testosterone stimulated the apoptosis of keratinocytes through the activation of caspase-3. The application of testosterone into 3-D skin equivalents increased the apoptosis of keratinocytes between the granular and stratified corneum layers. These results support an understanding of the molecular mechanism of testosterone-dependent apoptosis in which testosterone stimulates H2O2 generation through the activation of Duox1.
    The Journal of biological chemistry. 08/2014;
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    ABSTRACT: The complexity of end-of-life (EOL) communication in cancer care is often increased by family caregivers, who frequently affect the information and decision-making process. We assessed cancer patient preferences (PP), family caregiver preferences (FCP), and family caregiver predictions of patient preferences (FCPPP) regarding the disclosure of terminal status, family involvement in the disclosure process, and EOL choices, and we evaluated the concordances among them.
    Psycho-Oncology 08/2014; · 3.51 Impact Factor
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    ABSTRACT: Background Communication between cancer patients and caregivers is often suboptimal. The Cancer Communication Assessment Tool for Patient and Families (CCAT-PF) is a unique tool developed to measure congruence in patient–family caregiver communication employing a dyadic approach. We aimed to examine the cross-cultural applicability of the CCAT in the Korean healthcare setting.Methods Linguistic validation of the CCAT-PF was performed through a standard forward–backward translation process. Psychometric validation was performed with 990 patient–caregiver dyads recruited from 10 cancer centers.ResultsMean scores of CCAT-P and CCAT-F were similar at 44.8 for both scales. Mean CCAT-PF score was 23.7 (8.66). Concordance of each items between patients and caregivers was low (weighted kappa values <0.20 for all items and Spearman's rho <0.18 for scale scores). Scale scores did not differ significantly across a variety of cancer types and stages. The CCAT-P or CCAT-F score was weakly associated with mental health and quality of life outcomes. The CCAT-PF was correlated weakly with both patient-perceived and caregiver-perceived family avoidance of cancer care scales.Conclusion The CCAT-PF Korean version showed similar psychometric properties to the original English version in the assessment of communication congruence between cancer patient and family caregivers. Copyright © 2014 John Wiley & Sons, Ltd.
    Psycho-Oncology 07/2014; · 3.51 Impact Factor
  • Dong Wook Shin, Jong Hyock Park
    Cancer 07/2014; · 5.20 Impact Factor
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    ABSTRACT: Abstract Purpose: To investigate the health behaviors of people with retinitis pigmentosa (RP) in the Republic of Korea. Methods: An online questionnaire was used to survey members of the Korean Retinitis Pigmentosa Society (KRPS), over 3 months from December 2010 to February 2011. Controls were selected from participants of the 4th Korean National Health and Nutrition Examination Survey and were matched with people with RP using the propensity score method to optimize comparative analysis. A semi-structured interview was also conducted with five members of the KRPS to identify potential reasons for physical inactivity and unhealthy diet and to develop effective interventions. Results: A total of 194 eligible people with RP responded to the online survey, and 187 individuals were matched with the control population by propensity score matching. RP subjects reported a lower rate of current smoking and greater use of preventive healthcare services. However, people with RP were more physically inactive (50.8% vs 27.3%, p < 0.001) and consumed more fast food (69.5% vs 58.3%, p = 0.024) than the control population. Conclusion: Physical inactivity and fast food consumption were more frequently reported in people with RP than the general population. Efforts to understand the possible reasons and develop interventions to improve these health behaviors are warranted.
    Ophthalmic epidemiology. 06/2014;
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    ABSTRACT: Human epidermal γδ T cells are known to play crucial roles in the defense and homeostasis of the skin. However, their precise mechanism of action in skin inflammation remains less clear.
    Annals of Dermatology 06/2014; 26(3):308-13. · 0.61 Impact Factor
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    ABSTRACT: Decisions for cancer susceptibility genetic testing (CSGT) uptake and dissemination of results occur within the family context. A national survey was performed with 990 patient-family member dyads (participation rate:76.2%), with paired questionnaires examining attitudes toward CSGT uptake and disclosure of results in response to a hypothetical scenario in which a reliable cancer susceptibility genetic test was available for the specific cancer a patient was being treated. While most patients and family members responded they would uptake or recommend CSGT if available, concordance between the dyads was poor for both patient's testing (agreement rate 77.5%, weighted κ =0.09) and first-degree relatives' testing(agreement rate 78.0%, weighted κ =0.09). Most patients (93.2%) and family members (92.9 %) indicated that patients should disclose positive CSGT results to family members, with dyadic agreement of 89.1% (κ = 0.15). However, there were substantial disagreement regarding when disclosure should take place, who should make the disclosure (the patient or the health care professionals), and to whom the results should be disclosed. Patients and family members may hold different attitudes toward CSGT uptake of and disclosure of results within the family. Our findings reinforce the need for a family system approach to incorporate perspectives of patients as well as their family members.
    Clinical Genetics 01/2014; · 4.25 Impact Factor
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    ABSTRACT: Many tissues of the human body encounter hyperosmotic stress. The effect of extracellular osmotic changes on melanin production has not yet been elucidated. In this study, we determined that hyperosmotic stress induced by organic osmolytes results in reduced melanin production in human melanoma MNT-1 cells. Under hyperosmotic stress, few pigmented mature melanosomes were detected, but there was an increase in swollen vacuoles. These vacuoles were stained with an anti-M6PR antibody that recognizes late endosomal components and with anti-TA99 and anti-HMB45 antibodies, implying that melanosome formation was affected by hyperosmotic stress. Electron microscopic analysis revealed that the M6PR-positive swollen vacuoles were multi-layered and contained melanized granules, and they produced melanin when L-DOPA was applied, indicating that these vacuoles were still capable of producing melanin, but the inner conditions were not compatible with melanin production. The vacuolation phenomenon induced by hyperosmotic conditions disappeared with treatment with the PI3K activator 740 Y-P, indicating that the PI3K pathway is affected by hyperosmotic conditions and is responsible for the proper formation and maturation of melanosomes. The microarray analysis showed alterations of the vesicle organization and transport under hyperosmotic stress. Our findings suggest that melanogenesis could be regulated by physiological conditions, such as osmotic pressure.
    PLoS ONE 01/2014; 9(8):e105965. · 3.73 Impact Factor
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    ABSTRACT: We demonstrate a simple electrospinning for preparing Si-carbon composite Nanofiber (NF) in which aciniform aggregates of Si particles are well encased by amorphous carbon. The Si-carbon composite NF exhibit a significantly improved electrochemical performance with a high specific capacity of 1250 mAh·g−1 and a superior cycling performance during 50 cycles at a rate of 0.2 C. More importantly, Si-carbon composite NF maintain about 70% of initial capacity at 0.2 C and an excellent cycling stability even at 25 times higher current density compared to the initial condition, proving that it has superb kinetics compared to ever reported Si or SiOx materials. The electrochemical superiority of Si-carbon composite NF can be attributed to amorphous carbon framework accommodating the inherent volume expansion of Si during lithiation as well as the enlarged contact area between active materials and conducting agent attributed to the morphological characteristics of its one dimensional (1D) nanostructure.
    Electrochimica Acta. 01/2014; 118:100–105.
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    ABSTRACT: Objective To investigate the potentially different relationship between family history (FH) of coronary heart disease (CHD) and carotid or coronary atherosclerosis. Methods Asymptomatic healthy Korean adults older than 30 years who received both coronary CTA and carotid USG as part of a self-referred health check-up were retrospectively investigated (N = 662). Multivariable logistic regression analysis was employed to investigate the relationship between FH of CHD and either coronary CTA or carotid USG results. Results Adjusted for major CVD risk factors, FH of CHD was significantly associated with presence of any plaque in coronary arteries (aOR 2.10, 95% CI 1.07–4.16) and significant coronary stenosis (aOR 4.92, 95% CI 1.58–15.4), but was not associated with presence of any plaque in carotid arteries (aOR 1.27, 95% CI 0.61–2.63) and increased carotid IMT (aOR 1.44, 95% CI 0.40–5.22). Addition of FH of CHD had significant incremental predictive value to models for any coronary plaque (AUC 0.781 vs. 0.786, p = 0.0351), and significant coronary stenosis (AUC 0.772 vs. 0.808, p = 0.0129), but not for any carotid plaque (AUC 0.748 vs. 0.748, p = 0.528), and increased carotid IMT (AUC 0.778 vs. 0.783, p = 0.591). Conclusion To our knowledge, our study is the first to show specific comparative evidence that FH of CHD is more strongly associated with coronary than with carotid atherosclerosis. Our results suggest FH of CHD adds predictive value specifically to coronary atherosclerosis, but not carotid atherosclerosis, and suggest the possibility that screening for coronary atherosclerosis (via CAC) among low to intermediate risk asymptomatic adults with FH of CHD may be beneficial, who otherwise would not be screened according to traditional risk algorithms.
    Atherosclerosis 01/2014; 233(2):584–589. · 3.71 Impact Factor
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    ABSTRACT: (-)-Epigallocatechin-3-O-gallate (EGCG) has long been known as a potent inducer of keratinocyte differentiation. Although its molecular mechanisms have been extensively studied, its actions on human skin remain to be elucidated. In this study, we demonstrated that methylated EGCG and EGCG increase the expression of klotho, and that klotho functions as a downstream target of EGCG and methylated EGCG in keratinocyte differentiation. We demonstrated that methylated EGCG3 and EGCG induce morphological changes in normal human epidermal keratinocytes (NHEKs) that are related to up-regulation of klotho expression. We also demonstrated that a klotho-induced keratinocyte differentiation marker in NHEKs is inhibited by H-89, a protein kinase (PKA) inhibitor. These results suggest that methylated EGCG and EGCG may function as inducers of keratinocyte differentiation via transcriptional regulation of the klotho protein.
    International Journal of Molecular Sciences 01/2014; 15(4):5749-61. · 2.46 Impact Factor
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    ABSTRACT: We assessed the accuracy of communication between doctors and patients by evaluating the consistency between patient perception of cancer stage and the medical records, and analyzed the most influential factors of incongruence among cancer patients at 10 cancer centers across Korea. Information was gathered from cancer patients at the National Cancer Center and nine regional cancer centers located in every province of Korea between 1 July 2008 and 31 August 2008. Data were analyzed using Pearson's χ2 test and multivariate logistic regression analysis. The stages of cancer reported by the 1,854 patients showed a low degree of congruence with the stages given in medical records (k = 0.35, P<0.001). Only 57.1% of the patients had accurate knowledge of their cancer stage. In total, 18.5% underestimated their stage of disease, and the more advanced the cancer stage, the more likely they were to underestimate it, in order of local (14.2%), regional (23.7%), and distant (51.6%). Logistic regression analysis showed that congruence was lower in patients with cervical cancer (odds ratio [OR] = 0.51, 95% confidence interval [CI] = 0.30-0.87), recurrence (OR = 0.64, 95% CI = 0.50-0.83), and treatment at the National Cancer Center (OR = 0.53, 95% CI = 0.39-0.72). There are knowledge gaps between patients' perceived and actual stage of cancer. Patients with cervical cancer, recurrence, and who received treatment at a regional cancer center showed less understanding of their cancer stage.
    PLoS ONE 01/2014; 9(5):e90483. · 3.73 Impact Factor
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    ABSTRACT: This study aims to assess the current status of shared decision-making on instituting postmenopausal hormone therapy (HT). Two cross-sectional nationwide surveys of postmenopausal women and primary care physicians in the Republic of Korea were conducted in 2012 via face-to-face interviews. A total of 685 women (aged 50-69 y) who with natural menopause and 250 primary care physicians were included. Only 56.8% of primary care physicians reported that they explain the benefits and risks of HT and leave the decision to postmenopausal women. The others usually recommended using or not using HT. Of those postmenopausal women who had discussed such therapy with physicians (147 of 685; 21.5%), not all were aware of breast cancer or cardiovascular risks (only 65.3% and 38.8% were informed, respectively). Although most physicians perceived HT as beneficial for menopausal symptom control (99.6%) and acknowledged the related risk of breast cancer (84.8%), nearly half had the impression that HT was preventive of cardiovascular diseases. The interviewed women were less informed of the benefits and risks of HT than were the physician respondents. The awareness levels of the treated and untreated women did not differ. Participation of postmenopausal women in deciding whether to use HT is not prevalent. Physician-woman information transfer is suboptimal, and treatment decisions often are not based on the best available evidence. The current status of shared decision-making in this setting is clearly in need of improvement.
    Menopause (New York, N.Y.) 12/2013; · 3.08 Impact Factor
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    ABSTRACT: We aimed to identify the prevalence of feelings of guilt, censure, and concealment of smoking status among cancer patients and their family members who continued to smoke after the patient's diagnosis. Among 990 patient-family member dyads, 45 patients and 173 family members who continued to smoke for at least 1 month after the patients' diagnoses were administered questions examining feelings of guilt, censure, and smoking concealment. Most patients who continued to smoke reported experiencing feelings of guilt toward their families (75.6%) and censure from their family members (77.8%), and many concealed their smoking from their family members (44.4%) or healthcare professionals (46.7%). Family members who continued to smoke also reported feelings of guilt with respect to the patient (63.6%) and that the patient was critical of them (68.9%), and many concealed their smoking from the patient (28.5%) or healthcare professionals (9.3%). Patients' feeling of guilt was associated with concealment of smoking from family members (55.9% vs. 10.0%) or health care professionals (55.9% vs. 20.0%). Family members who reported feeling guilty (36.5% vs. 16.3%) or censured (34.5% vs. 16.7%) were more likely to conceal smoking from patients. Many patients and family members continue to smoke following cancer diagnosis, and the majority of them experience feelings of guilt and censure, which can lead to the concealment of smoking status from families or health care professionals. Feelings of guilt, censure, and concealment of smoking should be considered in the development and implementation of smoking cessation programs for cancer patients and family members. Copyright © 2013 John Wiley & Sons, Ltd.
    Psycho-Oncology 12/2013; · 3.51 Impact Factor
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    ABSTRACT: We verified the reliability and validity of the Korean version of the Minneapolis-Manchester Quality of Life Instrument-Adolescent Form (KMMQL-AF) among Korean childhood cancer survivors. A total of 107 childhood cancer patients undergoing cancer treatment and 98 childhood cancer survivors who completed cancer treatment were recruited. To assess the internal structure of the KMMQL-AF, we performed multi-trait scaling analyses and exploratory factor analysis. Additionally, we compared each domains of the KMMQL-AF with those of the Karnofsky Performance Status Scale and the Revised Children's Manifest Anxiety Scale (RCMAS). Internal consistency of the KMMQL-AF was sufficient (Cronbach's alpha: 0.78-0.92). In multi-trait scaling analyses, the KMMQL-AF showed sufficient construct validity. The "physical functioning" domain showed moderate correlation with Karnofsky scores and the "psychological functioning" domain showed moderate-to-high correlation with the RCMAS. The KMMQL-AF discriminated between subgroups of different adolescent cancer survivors depending on treatment completion. The KMMQL-AF is a sufficiently reliable and valid instrument for measuring quality of life among Korean childhood cancer survivors.
    Journal of Korean medical science 12/2013; 28(12):1788-95. · 0.84 Impact Factor
  • Joong Hyun Shim, Tae Ryong Lee, Dong Wook Shin
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    ABSTRACT: Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O2 concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.
    Molecules and Cells 11/2013; · 2.21 Impact Factor
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    ABSTRACT: Objective: We evaluated the psychometric properties of the Korean version of the European Organization for Research and Treatment of Cancer QLQ-PR25 when applied to Korean prostate cancer (PC) patients. Methods: A total of 172 patients who underwent curative radical prostatectomy (RP) with or without adjuvant androgen deprivation therapy were asked to complete the Korean version of the EORTC QLQ-C30 and PR25 questionnaires 3 times (before and 3 and 6 months after RP). Psychometric evaluation of the questionnaire was conducted. Results: Multitrait scaling analysis showed satisfactory construct validity in most scales except for bowel symptoms and hormonal treatment-related symptoms. Internal consistency tested by Cronbach's α coefficient met the 0.70 criterion for the urinary symptom, sexual activity and sexual functioning scales at the all 3 time points. Known-group comparison analyses showed better quality-of-life (QOL) scores in patients with higher performance status, and higher hormonal treatment-related symptom scores in patients on hormonal treatment. Responsiveness to changes was in line with clinical implications over time after RP. Conclusions: Our results show that the EORTC QLQ-PR25 questionnaire has adequate levels in cross-cultural validity. The Korean version of the EORTC QLQ-PR25 is a generally reliable and robust instrument for the assessment of various QOL aspects that can be self-administered to Korean PC patients undergoing RP. © 2013 S. Karger AG, Basel.
    Oncology 11/2013; 85(5):299-305. · 2.17 Impact Factor
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    ABSTRACT: Transparent conducting films (TCF) made up from carbon nanotubes (CNTs) have a tremendous potential in replacing the indium tin oxide films. Compare to single wall CNTs multiwall CNTs are more metallic and are more suitable candidate for the TCF. In this letter we report the use of selectively grown mm-scale, few-wall, vertically aligned CNTs for the fabrication of TCF. Water-assisted chemical vapor deposition was used to grow the mm-scale CNTs within short growth time. A special post-growth water-vapor treatment allowed us to remove the catalyst-free CNT forest very easily from the substrate and use it for the further process. A filtration-wet transfer process was used to form the TCF. The TCF shows sheet resistance of 228 omega/sq. at 72% transparency (at 550 nm). The ratio of optical conductivity to dc conductivity was observed in between 0.21 to 0.25 for below 80% transmission.
    Journal of Nanoscience and Nanotechnology 11/2013; 13(11):7413-7. · 1.15 Impact Factor
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    ABSTRACT: American Heart Association (AHA) defined 7 cardiovascular health metrics for the general population to improve cardiovascular health in 2010: not smoking; having normal blood pressure; being physically active; normal body mass index, blood glucose, and total cholesterol levels; and eating a healthy diet. To investigate trends in cardiovascular health metrics in Korea, we used data from the third and fourth Korean National Health and Nutrition Examination Surveys. We defined seven cardiovascular health metrics similar to the one defined by AHA but physical activity, body mass index, and healthy diet were properly redefined to be suited for the Korean population. We compared each cardiovascular health metric and calculated the sum of cardiovascular health metrics after dichotomizing each health metric to ideal (scored 1) and poor (scored 0). Health metric scores of smoking in males (P value for trend < 0.001), physical activity both in males and females (P-value for trend < 0.001 both), body mass index in females (P-value for trend = 0.030), and blood pressure both in males and females (P-value for trend < 0.001, both) were improved. On the other hand, health metric scores of healthy diet in males (P-value for trend = 0.002), and fasting blood glucose both in males and females (P-value for trend < 0.001 both) got worse. The total scores of seven health metrics were stationary. Total scores were not changed but each metric showed various trends. A long-term study is necessary for analyzing exact trends.
    Korean Journal of Family Medicine 11/2013; 34(6):403-12.
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    ABSTRACT: Thin graphene/polymer sheet composites were fabricated using easily soluble expanded graphite (ESEG), and their field emission (FE) parameters were examined. Due to the high dispersability of ESEG, a stable graphene suspension was prepared by ultrasonication in toluene without the need for a surfactant. The suspension consisted of exfoliated graphene sheets with a thickness of 1 - 2 nm. Using a calendering process, the solution was further shear mixed with ethyl cellulose to obtain a well-dispersed graphene/polymer composite. The composite was screen printed onto a conducing substrate to fabricate the FE cathode layers. The FE measurements were taken in a diode configuration at an applied electrostatic field and inter-electrode distance of 1.7 to 6 V/microm and approximately 200 microm, respectively. The threshold turn-on-field was approximately 3.5 V/microm at a current density of approximately 10 microA/cm2 with a corresponding mean field enhancement factor of 1350 +/- 50. Emission occurred mainly from the edges and bends of the graphene layers. The luminescence uniformity of the composite cathode layers was examined using a phosphor-coated anode.
    Journal of Nanoscience and Nanotechnology 11/2013; 13(11):7689-94. · 1.15 Impact Factor

Publication Stats

464 Citations
367.69 Total Impact Points

Institutions

  • 2010–2014
    • Seoul National University Hospital
      • Department of Family Medicine
      Sŏul, Seoul, South Korea
  • 2008–2014
    • Amorepacific Corporation
      Sŏul, Seoul, South Korea
  • 2002–2014
    • Hanyang University
      • Division of Materials Science and Engineering (MSE)
      Sŏul, Seoul, South Korea
    • Pohang University of Science and Technology
      • Department of Materials Science and Engineering
      Andong, North Gyeongsang, South Korea
  • 2011–2013
    • University of Texas at Austin
      • Department of Materials Science and Engineering
      Austin, Texas, United States
  • 2009–2013
    • Sungkyunkwan University
      • School of Advanced Materials Science and Engineering (AMSE)
      Sŏul, Seoul, South Korea
    • Korea Advanced Institute of Science and Technology
      • Department of Mechanical Engineering
      Seoul, Seoul, South Korea
  • 2008–2013
    • National Cancer Center Korea
      • Gastric Cancer Branch
      Kōyō, Gyeonggi Province, South Korea
    • Korea Institute of Science and Technology
      • Electronic Materials Research Center
      Sŏul, Seoul, South Korea
  • 2012
    • Yonsei University Hospital
      Sŏul, Seoul, South Korea
    • CHA University
      Sŏul, Seoul, South Korea
  • 2005
    • Yonsei University
      Sŏul, Seoul, South Korea