D A Vuitton

University of Franche-Comté, Becoinson, Franche-Comté, France

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Publications (306)711.31 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Smad family proteins are essential cellular mediators of the transforming growth factor-β superfamily. In the present study, we identified two members of the Smad proteins, Smad8 and Smad4 homologues (termed as EgSmadE and EgSmadD, respectively), from Echinococcus granulosus, the causative agent of cystic echinococcosis (CE). Phylogenetic analysis placed EgSmadE in the Smad1, 5, and 8 subgroup of the R-Smad sub-family and EgSmadD in the Co-Smad family. Furthermore, EgSmadE and EgSmadD attained a high homology to EmSmadE and EmSmadD of E. multilocularis, respectively. Both EgSmadE and EgSmadD were co-expressed in the larval stages and exhibited the highest transcript levels in activated protoscoleces, and their encoded proteins were co-localized in the sub-tegumental and tegumental layer of the parasite. As shown by yeast two-hybrid and pull-down analysis, EgSmadE displayed a positive binding interaction with EgSmadD. In addition, EgSmadE localized in the nuclei of Mv1Lu cells (mink lung epithelial cells) upon treatment with human TGF-β1 or human BMP2, indicating that EgSmadE is capable of being translocated into nucleus, in vitro. Our study suggests that EgSmadE and EgSmadD may take part in critical biological processes, including echinococcal growth, development, and parasite-host interaction.
    Parasitology research. 07/2014;
  • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 07/2014;
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    ABSTRACT: Echinococcosis is a major parasitic zoonosis of public health importance in western China. In 2004, the Chinese Ministry of Health estimated that 380,000 people had the disease in the region. The Qinghai-Tibet Plateau is highly co-endemic with both alveolar echinococcosis (AE) and cystic echinococcosis (CE). In the past years, the Chinese government has been increasing the financial support to control the diseases in this region. Therefore, it is very important to identify the significant risk factors of the diseases by reviewing studies done in the region in the past decade to help policymakers design appropriate control strategies.Review: Selection criteria for which literature to review were firstly defined. Medline, CNKI (China National Knowledge Infrastructure), and Google Scholar were systematically searched for literature published between January 2000 and July 2011. Significant risk factors found by single factor and/or multiple factors analysis were listed, counted, and summarized. Literature was examined to check the comparability of the data; age and sex specific prevalence with same data structures were merged and used for further analysis.A variety of assumed social, economical, behavioral, and ecological risk factors were studied on the Plateau. Those most at risk were Tibetan herdsmen, the old and female in particular. By analyzing merged comparable data, it was found that females had a significant higher prevalence, and a positive linearity relationship existed between echinococcosis prevalence and increasing age. In terms of behavioral risk factors, playing with dogs was mostly correlated with CE and/or AE prevalence. In terms of hygiene, employing ground water as the drinking water source was significantly correlated with CE and AE prevalence. For definitive hosts, dog related factors were most frequently identified with prevalence of CE or/and AE; fox was a potential risk factor for AE prevalence only. Overgrazing and deforestation were significant for AE prevalence only. Tibetan herdsmen communities were at the highest risk of echinococcosis prevalence and should be the focus of echinococcosis control. Deworming both owned and stray dogs should be a major measure for controlling echinococcosis; treatment of wild definitive hosts should also be considered for AE endemic areas. Health education activities should be in concert with the local people's education backgrounds and languages in order to be able to improve behaviors. Further researches are needed to clarify the importance of wild hosts for AE/CE prevalence, the extent and range of the impacts of ecologic changes (overgrazing and deforestation) on the AE prevalence, and risk factors in Tibet.
    Infectious diseases of poverty. 01/2014; 3(1):3.
  • Revue Française d'Allergologie 01/2014; 54(3):227. · 0.35 Impact Factor
  • Médecine Nucléaire. 01/2014; 38(3):143.
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    ABSTRACT: Pathogenesis of chronically developing alveolar echinococcosis (AE) is characterized by a continuous, granulomatous, periparasitic infiltration of immune cells surrounding the metacestode of Echinococcus multilocularis (E.multilocularis) in the affected liver. A detailed cytokine and chemokine profile analysis of the periparasitic infiltrate in the liver has, however, not yet been carried out in a comprehensive way all along the whole course of infection in E. multilocularis intermediate hosts. We thus assessed the hepatic gene expression profiles of 18 selected cytokine and chemokine genes using qRT-PCR in the periparasitic immune reaction and the subsequent adjacent, not directly affected, liver tissue of mice from day 2 to day 360 post intra-hepatic injection of metacestode. DNA microarray analysis was also used to get a more complete picture of the transcriptional changes occurring in the liver surrounding the parasitic lesions. Profiles of mRNA expression levels in the hepatic parasitic lesions showed that a mixed Th1/Th2 immune response, characterized by the concomitant presence of IL-12α, IFN-γ and IL-4, was established very early in the development of E. multilocularis. Subsequently, the profile extended to a combined tolerogenic profile associating IL-5, IL-10 and TGF-β. IL-17 was permanently expressed in the liver, mostly in the periparasitic infiltrate; this was confirmed by the increased mRNA expression of both IL-17A and IL-17F from a very early stage, with a subsequent decrease of IL-17A after this first initial rise. All measured chemokines were significantly expressed at a given stage of infection; their expression paralleled that of the corresponding Th1, Th2 or Th17 cytokines. In addition to giving a comprehensive insight in the time course of cytokines and chemokines in E. multilocularis lesion, this study contributes to identify new targets for possible immune therapy to minimize E. multilocularis-related pathology and to complement the only parasitostatic effect of benzimidazoles in AE.
    PLoS ONE 01/2014; 9(3):e91638. · 3.53 Impact Factor
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    Parasite (Paris, France) 01/2014; 21:28. · 1.12 Impact Factor
  • Dominique A Vuitton, Solange Bresson-Hadni
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    ABSTRACT: Introduction: Care management of patients with alveolar echinococcosis (AE), a rare, severe and orphan parasitic disease, is essentially based on expert consensus. Treatment strategies and recommendations have regularly been updated by the WHO-Informal Working Group on Echinococcosis, based upon the 35 years’ experience, research and published papers from the main reference centers in Europe, Japan and China. Areas covered: This expert opinion covers the basics of benzimidazole chemotherapy and interventions used in the care management of patients with AE, and it summarizes the current status of potentially ‘candidate’ drugs. It closely follows and updates the statements of the last ‘expert consensus’ published in 2010, which includes radical resection of liver lesions whenever possible, and continuous and prolonged treatment with albendazole (ABZ) or mebendazole (MBZ) in all patients. It discusses the modalities of patient’s follow-up and of a possible benzimidazole withdrawal in selected patients. Expert opinion: The authors of this review are convinced that for such a disease, organization of care management and involvement of the patient in his/her own care are as important as technical skills and drug efficacy. It is why they propose networking and multidisciplinary approach as a basis for progress. This should allow the constitution of well-characterized multicenter cohorts of patients and the implementation of prospective controlled studies. They also provide some practical recommendations that reflect the current practice in their reference center, especially regarding treatment initiation and follow-up, and the management of ABZ adverse effects.
    Expert Opinion on Orphan Drugs. 12/2013; 2(1).
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    ABSTRACT: Flavonoids have antitumoral properties and may be attractive candidates as anticancer therapy. Isoliquiritigenin which is a constituent of licorice (Glycyrrhiza inflata), a plant commonly used in traditional Uyghur medicine in Xinjiang, China, was studied for antiproliferative and apoptotic activity in human cervical cancer cells, Ca Ski, SiHa, HeLa, and C-33A. Its molecular mechanism of action was specifically examined in Ca Ski cells. Isoliquiritigenin decreased cell viability, induced cell accumulation in G2/M and morphological and biochemical features of apoptosis in the four cancer cell lines. In Ca Ski cells, isoliquiritigenin led to a downregulation of HPV16 E6 expression associated with an increase of p53 and p21 levels, enhanced expression of Bax and decreased expression of Bcl-2 and Bid proform triggering dissipation of the mitochondrial membrane potential, released cytochrome c to the cytosol followed by activation of caspase cascade with cleavage of caspase-9, caspase-3, and PARP. Caspase-8 was also cleaved. Moreover treatment with a pan-caspase inhibitor prevented apoptosis. As Ca Ski cells are representative of carcinoma naturally occurring in the cervix, our results suggest a potential benefit of isoliquiritigenin for cervical cancer prevention and treatment.
    Planta Medica 11/2013; · 2.35 Impact Factor
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    ABSTRACT: European cross-sectional studies have suggested that prenatal and postnatal farm exposure decreases the risk of allergic diseases in childhood. Underlying immunologic mechanisms are still not understood but might be modulated by immune-regulatory cells early in life, such as regulatory T (Treg) cells. We sought to assess whether Treg cells from 4.5-year-old children from the Protection against Allergy: Study in Rural Environments birth cohort study are critical in the atopy and asthma-protective effect of farm exposure and which specific exposures might be relevant. From 1133 children, 298 children were included in this study (149 farm and 149 reference children). Detailed questionnaires until 4 years of age assessed farming exposures over time. Treg cells were characterized as upper 20% CD4(+)CD25(+) forkhead box protein 3 (FOXP3)(+) (intracellular) in PBMCs before and after stimulation (with phorbol 12-myristate 13-acetate/ionomycin or LPS), and FOXP3 demethylation was assessed. Atopic sensitization was defined by specific IgE measurements; asthma was defined by a doctor's diagnosis. Treg cells were significantly increased in farm-exposed children after phorbol 12-myristate 13-acetate/ionomycin and LPS stimulation. Exposure to farm milk was defined as a relevant independent farm-related exposure supported by higher FOXP3 demethylation. Treg cell (upper 20% CD4(+)CD25(+), FOXP3(+) T cells) numbers were significantly negatively associated with doctor-diagnosed asthma (LPS stimulated: adjusted odds ratio, 0.26; 95% CI, 0.08-0.88) and perennial IgE (unstimulated: adjusted odds ratio, 0.21; 95% CI, 0.08-0.59). Protection against asthma by farm milk exposure was partially mediated by Treg cells. Farm milk exposure was associated with increased Treg cell numbers on stimulation in 4.5-year-old children and might induce a regulatory phenotype early in life, potentially contributing to a protective effect for the development of childhood allergic diseases.
    The Journal of allergy and clinical immunology 08/2013; · 12.05 Impact Factor
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    ABSTRACT: SUMMARY Recent changes in the epidemiology of alveolar echinococcosis (AE) in Eurasia have led to increasing concerns about the risk of human AE and the need for a thorough evaluation of the epidemiological situation. The aim of this study was to explore the use of a National Register to detect complex distribution patterns on several scales. The data were human AE cases from the FrancEchino register, diagnosed in France from 1982 to 2011. We used the Kulldorff spatial scan analysis to detect non-random locations of cases. We proposed an exploratory method that was based on the successive detection of nested clusters inside each of the statistically significant larger clusters. This method revealed at least 4 levels of disease clusters during the study period. The spatial variations of cluster location over time were also shown. We conclude that National Human AE registers, although not exempted from epidemiological biases, are currently the best way to achieve an accurate representation of human AE distribution on various scales. Finally, we confirm the multi-scale clustered distribution of human AE, and we hypothesize that our study may be a reasonable starting point from which to conduct additional research and explore the processes that underlie such distributions.
    Parasitology 08/2013; · 2.36 Impact Factor
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    ABSTRACT: (18)F-FDG PET has already proved its usefulness in the follow-up of patients with alveolar echinococcosis (AE) and has been proposed as a surrogate marker for therapeutic decisions on structured treatment interruption by benzimidazoles. However, standard PET acquisition (1 h after (18)F-FDG injection) lacks sensitivity, and the parasite may stay viable even if (18)F-FDG perilesional uptake has disappeared. The aim of our study was to evaluate the usefulness of delayed (18)F-FDG PET in the management of AE patients. METHODS: During a 6-y period, 120 PET scans using (18)F-FDG were obtained for 70 AE patients treated by benzimidazoles, without selection. All patients underwent whole-body imaging on a PET/CT device 1 h after (18)F-FDG injection (4 MBq/kg), as well as an acquisition focused on the liver 3 h after the injection. We also analyzed the results of serologic tests. RESULTS: Of the 57 scans considered negative at the standard acquisition, 13 (22.8%) became clearly positive at the delayed acquisition, and 6 (10.5%) became indeterminate at the delayed acquisition. Furthermore, 20 of 22 scans interpreted as indeterminate at the standard acquisition were considered positive because of clear perilesional (18)F-FDG uptake at the delayed acquisition. Thus, delayed acquisition changed the interpretation in 32.5% of cases. Moreover, of 44 patients treated by benzimidazoles and followed for more than 2 y by regular (18)F-FDG PET scans and specific AE serology, 11 (25%) presented pathologic (18)F-FDG uptake at the delayed acquisition but not at the standard one. In these patients, the treatment was continued despite negative results on standard (18)F-FDG PET and negative serologic findings. On the other hand, in 7 patients with negative delayed (18)F-FDG PET and negative serology, the treatment was safely interrupted with no evidence of disease recurrence during 8-37 mo (mean, 23 mo). CONCLUSION: Our study clearly demonstrated that delayed (18)F-FDG PET greatly facilitated the differentiation between active and inactive liver lesions in AE patients. Also, our results strongly suggested that the combination of delayed (18)F-FDG PET and specific serology would prevent most of the recurrences observed after premature interruption of the treatment based only on standard (18)F-FDG PET.
    Journal of Nuclear Medicine 01/2013; · 5.77 Impact Factor
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    ABSTRACT: We report the 30-yr history of a well-documented human case of alveolar echinococcosis, with a lung lesion at presentation followed by the discovery of a liver lesion, both removed by surgery. Subsequently, within the 13 years following diagnosis, metastases were disclosed in eye, brain and skull, as well as additional lung lesions. This patient had no immune suppression, and did not have the genetic background known to predispose to severe alveolar echinococcosis; it may thus be hypothesized that iterative multi-organ involvement was mostly due to the poor adherence to benzimidazole treatment for the first decade after diagnosis. Conversely, after a new alveolar echinococcosis recurrence was found in the right lung in 1994, the patient accepted to take albendazole continuously at the right dosage. After serology became negative and a fluoro-deoxy-glucose-Positron Emission Tomography performed in 2005 showed a total regression of the lesions in all organs, albendazole treatment could be definitively withdrawn. In 2011, the fluoro-deoxy-glucose-Positron Emission Tomography showed a total absence of parasitic metabolic activity and the patient had no clinical symptoms related to alveolar echinococcosis.The history of this patient suggests that multi-organ involvement and alveolar echinococcosis recurrence over time may occur in non-immune suppressed patients despite an apparently "radical" surgery. Metastatic dissemination might be favored by a poor adherence to chemotherapy. Combined surgery and continuous administration of albendazole at high dosage may allow alveolar echinococcosis patients to survive more than 30 years after diagnosis despite multi-organ involvement.
    Annals of Clinical Microbiology and Antimicrobials 01/2013; 12(1):1. · 1.62 Impact Factor
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    ABSTRACT: Alveolar echinococcosis (AE) is characterized by the development of irreversible fibrosis and of immune tolerance towards Echinococcus multilocularis (E. multilocularis). Very little is known on the presence of transforming growth factor-β (TGF-β) and other components of TGF-β/Smad pathway in the liver, and on their possible influence on fibrosis, over the various stages of infection. Using Western Blot, qRT-PCR and immunohistochemistry, we measured the levels of TGF-β1, TGF-β receptors, and down-stream Smads activation, as well as fibrosis marker expression in both a murine AE model from day 2 to 360 post-infection (p.i.) and in AE patients. TGF-β1, its receptors, and down-stream Smads were markedly expressed in the periparasitic infiltrate and also in the hepatocytes, close to and distant from AE lesions. Fibrosis was significant at 180 days p.i. in the periparasitic infiltrate and was also present in the liver parenchyma, even distant from the lesions. Over the time course after infection TGF-β1 expression was correlated with CD4/CD8 T-cell ratio long described as a hallmark of AE severity. The time course of the various actors of the TGF-β/Smad system in the in vivo mouse model as well as down-regulation of Smad7 in liver areas close to the lesions in human cases highly suggest that TGF-β plays an important role in AE both in immune tolerance against the parasite and in liver fibrosis.
    PLoS ONE 01/2013; 8(2):e55379. · 3.53 Impact Factor
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    ABSTRACT: Human alveolar echinococcosis (AE) is a parasitic zoonosis with intermediate (rodents and lagomorphs) and final hosts (carnivores, mainly fox but also dogs). The latter can transmit the parasite to humans, by their feces, which are contaminated by the oncospheres of Echinococcus multilocularis, the larvae of which develop in the liver like a slow cancer. This chronically progressive disease may involve adjacent tissues and organs. Distant metastases may also be observed. Infected patient are frequently detected at earlier asymptomatic stage. Increasing fox populations, their invasion of the cities and the detection of the parasite in foxes from previously parasite-free areas seem to currently modify the epidemiology of AE. Patients’ management must be multidisciplinary and includes (i) radical liver resection whenever possible, (ii) avoidance of palliative surgical procedures, (iii) use of interventional radiology or endoscopy to treat biliary and vascular complications whenever possible, (iv) indication of liver transplantation only in patients with life-threatening complications, (v) treatment of all patients without exception by albendazole for at least two years after radical surgery and long-term (at least several years) in non operated cases, (vi) use of positron-emission tomography combined with computed tomography (PET-CT) and specific serological tools to assess the functional activity of the lesions and monitor treatment, (vii) regular monitoring of albendazole plasmatic levels. Patients’ survival and quality of life have improved very substantially, due to optimized care strategy.
    Journal des Anti-infectieux. 01/2013;
  • Journal of the American College of Cardiology 07/2012; 60(1):81-2; author reply 82-3. · 14.09 Impact Factor
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    ABSTRACT: La démarche qualité (« décrire, écrire, analyser ») génère inévitablement la rédaction de textes censés améliorer la communication au sein d’une équipe et être opposables en cas d’accident. Or, en dépit d’une gestion documentaire performante, la qualité des textes eux-mêmes est parfois laissée pour compte : les langues naturelles foisonnent d’ambigüités et les professionnels de santé perçoivent la rédaction comme une contrainte ; de plus, le consensus sur le contenu est souvent difficile à obtenir. Une Langue Contrôlée (LC) est un sous-ensemble d’une langue naturelle dont la grammaire et le lexique ont été restreints pour assurer la ompréhensibilité et/ou faciliter la traduction d’un texte. Dans le cadre du programme « Sécurité Globale » de l’Agence Nationale de la Recherche (ANR), nous avons donc conçu une méthodologie de LC qui permet de développer des LC sur mesure, adaptées au domaine d’activité, au type de texte (protocoles, notices, messages d’alerte, etc.), au public cible, au degré d’urgence, aux besoins d’outils de traitement automatique des langues. Notre recherche s’est appuyée sur l’analyse d’un corpus de textes et sur une collaboration entre linguistes, professionnels de santé et responsables qualité d’établissements de santé. Nous avons relevé nombre de coutumes rédactionnelles nuisibles à la transmission du message, dont voici quelques exemples : emploi immodéré de la forme passive appliquée à un contenu injonctif, incohérences chronologiques, usage de termes ambigus, mention des exceptions après le cas général. Nous avons ensuite appliqué notre méthodologie à 2 domaines de santé (immunobiologie et radio-protection) dans le cadre du projet Sensunique (Programme « Émergence » de l’ANR). Par ailleurs, nous avons mis au point un logiciel d’aide à la conception et à la rédaction de textes en LC. Issu d’un compromis entre les besoins du rédacteur et les exigences de la LC, cet outil assiste le rédacteur, assurant le respect des règles de rédaction sans pour autant attendre de lui des connaissances approfondies en linguistique, et permet la rédaction collaborative de textes en LC.
    4e Rencontre des métiers de lasanté à Strasbourg : Qualité & Risque, Strasbourg, France; 05/2012
  • Revue Française d'Allergologie 04/2012; 52(3):269. · 0.35 Impact Factor
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    ABSTRACT: Positron emission tomography (PET) with 18F-fluorodeoxyglucose (18F-FDG) can be used to evaluate parasitic activity in alveolar echinococcosis (AE) as visualization of host–parasite immune reaction around the lesions. We performed a retrospective interpretation of PET and MR (magnetic resonance) images in 24 patients with AE to compare the results of metabolic imaging to Kodama et al. anatomical classification. All patients underwent hepatic MRI and dual-time point FDG-PET (1 hour and 3 hours after injection), improving sensitivity of standard FDG-PET in AE. The comparison of the images showed perilesional 18F-FDG uptake in all seven patients with type 1 (multiple microcysts without a solid component) and type 2 (multiple microcysts with a solid component) lesions and in ten of 12 patients with type 3 lesions (a solid component surrounding cysts and microcysts). No abnormal perilesional uptake was observed at standard and delayed PET acquisitions in two of 12 patients with type 3 lesions and in all five patients with type 4 (a solid component without microcysts) and type 5 (a large liquid cavity without microcysts) lesions. Our study demonstrates a strong correlation between anatomical “microcystic” aspect and metabolic activity of AE lesions in 89.5% of cases. Thus, microcysts and metabolic perilesional activity seem correlated to parasite activity, which suggests an important role to combined follow-up of AE patients, through multimodality imaging using MRI and 18F-FDG-PET scan.
    Médecine Nucléaire. 02/2012; 36(2):77–82.
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    ABSTRACT: Alveolar echinococcosis (AE) is characterized by the tumor-like growth of Echinococcus (E.) multilocularis. Very little is known on the influence of helminth parasites which develop in the liver on the proliferation/growth arrest metabolic pathways in the hepatocytes of the infected liver over the various stages of infection. Using Western blot analysis, qPCR and immunohistochemistry, we measured the levels of MAPKs activation, Cyclins, PCNA, Gadd45β, Gadd45γ, p53 and p21 expression in the murine AE model, from day 2 to 360 post-infection. Within the early (day 2-60) and middle (day60-180) stages, CyclinB1 and CyclinD1 gene expression increased up to day30 and then returned to control level after day60; Gadd45β, CyclinA and PCNA increased all over the period; ERK1/2 was permanently activated. Meanwhile, p53, p21 and Gadd45γ gene expression, and caspase 3 activation, gradually increased in a time-dependent manner. In the late stage (day180-360), p53, p21 and Gadd45γ gene expression were significantly higher in infected mice; JNK and caspase 3 were activated. TUNEL analysis showed apoptosis of hepatocytes. No significant change in CyclinE, p53 mRNA and p-p38 expression were observed at any time. Our data support the concept of a sequential activation of metabolic pathways which 1) would first favor parasitic, liver and immune cell proliferation and survival, and thus promote metacestode fertility and tolerance by the host, and 2) would then favor liver damage/apoptosis, impairment in protein synthesis and xenobiotic metabolism, as well as promote immune deficiency, and thus contribute to the dissemination of the protoscoleces after metacestode fertility has been acquired. These findings give a rational explanation to the clinical observations of hepatomegaly and of unexpected survival of AE patients after major hepatic resections, and of chronic liver injury, necrosis and of hepatic failure at an advanced stage and in experimental animals.
    PLoS ONE 01/2012; 7(1):e30127. · 3.53 Impact Factor

Publication Stats

3k Citations
711.31 Total Impact Points

Institutions

  • 1991–2014
    • University of Franche-Comté
      Becoinson, Franche-Comté, France
  • 2000–2011
    • Xinjiang Medical University
      Ouroumtchi, Xinjiang Uygur Zizhiqu, China
  • 2006–2010
    • Sichuan Center for Disease Control and Prevention
      Hua-yang, Sichuan, China
    • Queensland Institute of Medical Research
      • Molecular Parasitology Laboratory
      Brisbane, Queensland, Australia
    • Universitätsklinikum Freiburg
      • Division of Infectious Diseases
      Freiburg, Lower Saxony, Germany
  • 2009
    • University of Pavia
      • Department of Public Health, Neuroscience, Experimental and Forensic Medicine
      Pavia, Lombardy, Italy
  • 1984–2008
    • Centre Hospitalier Régional et Universitaire de Besançon
      Becoinson, Franche-Comté, France
  • 2003
    • University of Constantine 1
      Cirta, Constantine, Algeria
    • Shenzhen Second People's Hospital
      Shen-ch’üan-shih, Zhejiang Sheng, China
  • 1995–2003
    • University of Salford
      • School of Environment and Life Sciences
      Salford, England, United Kingdom
    • CHRU de Strasbourg
      Strasburg, Alsace, France
    • Association Française pour la Recherche sur l'Hidrosadénite
      Lutetia Parisorum, Île-de-France, France
  • 2002
    • Asahikawa Medical University
      • Department of Parasitology
      Asakhigava, Hokkaidō, Japan
  • 1985–1998
    • Institut Pasteur
      Lutetia Parisorum, Île-de-France, France
  • 1997
    • Claude Bernard University Lyon 1
      Villeurbanne, Rhône-Alpes, France
  • 1996
    • French National Centre for Scientific Research
      Lutetia Parisorum, Île-de-France, France