D E Korzhevskiĭ

Russian Academy of Medical Sciences, Moskva, Moscow, Russia

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Publications (61)0.05 Total impact

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    ABSTRACT: The results of examination and surgical treatment of 298 patients with chronic pancreatitis and the original morphological investigations of material of the pancreas were studied. The data allowed the detection of additional criteria features of inclusion of the patients with chronic pancreatitis to be made in groups according to foreign Marseilles-Roman classification (1988). It is shown, that the basis of study of morphogenesis of chronic pancreatitis is immunohistochemical method, which let the authors diagnose not only the pathological changes of exo- and endocrine sections of pancreas, but at the same time the structural features of nervous apparatus and vessels of microcircular bed. The revealed morphological features of different forms of chronic pancreatitis vs clinical finding characteristics and the data of instrumental and laboratory methods of research allow the substantiation of surgical treatment version to be made.
    Vestnik khirurgii imeni I. I. Grekova 01/2013; 172(4):29-39.
  • D E Korzhevskiĭ · E G Sukhorukova · I P Grigor'ev
    Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova / Ministerstvo zdravookhraneniia i meditsinskoi promyshlennosti Rossiiskoi Federatsii, Vserossiiskoe obshchestvo nevrologov [i] Vserossiiskoe obshchestvo psikhiatrov 01/2013; 113(6):77-80. · 0.05 Impact Factor
  • L I Khozhaĭ · V A Otellin · D E Korzhevskiĭ
    Zhurnal evoliutsionnoĭ biokhimii i fiziologii 05/2012; 48(3):293-5. DOI:10.1134/S0022093012030139
  • O V Kirik · I P Grigor'ev · E G Sukhorukova · N V Pavlova · D E Korzhevskiĭ
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    ABSTRACT: Forebrain subventricular zone (SVZ)--the putative major source of neural stem cells in the brain of adult mammals--can hardly be visualized using routine histological staining. The present study was focused on the possibility of application of immunocytochemical approach for accurate delineation of the border between SVZ and striatum. It was shown that immunocytochemical reactions demonstrating tyrosine hydroxylase or synaptophysin were optimal for the determination of the border between SVZ and striatum in different mammals.
    Morfologiia (Saint Petersburg, Russia) 01/2012; 141(1):81-4.
  • D E Korzhevskiĭ · O V Kirik · E G Sukhorukova · T D Vlasov
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    ABSTRACT: Microgliocytes are currently known as structurally labile cells, that, by changing the length of their processes, control the synaptic organization of the brain. The aim of this study was to investigate structural organization of microglia of the striatum and of the forebrain periventricular area in rat following transitory focal ischemia. In the absence of brain infarction, ischemic lesion was found to activate microglia, which contributed to rearrangement of striatal neuropil. As microgliocytes became activated, they changed their shape from characteristic dendritic one to oval and rounded, which are typical to amoeboid microglia. Microgliocytes were shown to be capable of proliferation.
    Morfologiia (Saint Petersburg, Russia) 01/2012; 141(2):28-32.
  • I P Grigor'ev · E G Sukhorukova · E A Kolos · D E Korzhevskiĭ
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    ABSTRACT: The distribution of tyrosine hydroxylase (TH), a key enzyme of catecholamine synthesis, was studied immunocytochemically in the neurons of substantia nigra in the human brain (n=7), and localization of neuromelanin in these cells was determined. The evidence indicating the existence of three types of neurons in substantia nigra was obtained, including the neurons containing both TH and neuromelanin, neurons containing only neuromelanin, and neurons expressing neither TH, nor neuromelanin. Presence of a population of neuromelanin-containing neurons lacking TH (which make up 7-30% of the cells) is discussed considering the participation of catecholamines and TH in neuromelanin synthesis.
    Morfologiia (Saint Petersburg, Russia) 01/2012; 141(2):65-7. DOI:10.1007/s11055-013-9755-7
  • E G Sukhorukova · O S Alekseeva · O V Kirik · N A Grudinina · D E Korzhevskiĭ
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    ABSTRACT: A study of structural and functional organization of the boundaries separating CNS compartments is a fundamental task of neurobiology. Taking into account the contradictory data on the structure of superficial layers of mammalian neocortex, it is pertinent to study structural and cytochemical organization of astrocytes--the main components of the brain barrier system in animals that are often used for experimental modeling of brain diseases and injuries. The aim of the present work was to study the structural organization of layer I astrocytes of rat neocortex. Astrocytes were demonstrated immunocytochemically using anti-GFAP, anti-vimentin and anti-nestin antibodies using light and confocal laser microscopy. The results of the study demonstrated that the superficial glial limiting membrane had significant structural differences in different cortical regions. Astrocytes in layer I of rat neocortex were different from typical protoplasmic astrocytes, common to gray matter The regional peculiar features of superficial glial limiting membrane organization that were found in this study, are probably determined by the differences in functional characteristics of CSF-encephalic barrier in the specific regions of the brain.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 140(6):32-5.
  • E I Chumasov · P A Voronchikhin · D E Korzhevskiĭ
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    ABSTRACT: The pulmonary vein (PV) of 16 adult male Wistar rat was studied using synaptophysin immunohistochemistry and staining with toluidine blue and hematoxylin. Its intrapulmonary trunk wall was found to have a unique structure. The middle tunic contained cross-striated muscle tissue that was identical to the cardiac muscle tissue in posterior wall of the myocardial atrium. The thickness of the muscular tunic increased with the increase of vein diameter. The thickest layer of cardiac muscle fibers was located in the orifice of the main trunk, and the thinnest - in the lateral veins (50-100 microm in diameter), carrying the arterial blood from the respiratory portion of pulmonary lobe. Along their whole length, cardiac muscle fibers are densely innervated. Efferent synaptophysin-positive endings of the terminal plexus were found to be tightly associated with cardiac muscle fibers, capillaries of the media in the area of PV orifice and vasa vasorum of the adventitia.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 140(6):53-5.
  • D E Korzhevskiĭ · M N Karpenko · O V Kirik
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    ABSTRACT: This paper is aimed at the characterization of beta-III-tubulin, MAP2 and doublecortin, the proteins which participate in the organization, stabilization and function of the microtubules of nerve cell cytoskeleton. Due to the structural and functional features, these proteins may be regarded as differentiation markers, associated with neurogenesis and as the indicators of nerve cell functional status under normal and pathological conditions. The data presented show that these proteins perform important structural and transport functions in nerve cells and are essential for some neurospecific intracellular processes. However, current knowledge of the functional role of these proteins in nerve cells is insufficient and requires significant supplementation indispensable for unequivocal interpretation of the studies results.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 139(1):13-21.
  • O V Kirik · O S Alekseeva · D É Korzhevskiĭ
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    ABSTRACT: The aim of the study was to examine the distribution of cells expressing Msi-1 (Musashi-1) protein which is believed to be a marker of neural stem cells, in rat telencephalon. These cells were found to be concentrated in the subventricular proliferative zone and diffusely scattered in striatum and hippocampus. Moreover, neurons with extensive Msi-1 reaction were found in habenular nuclei. The data obtained are not in full agreement with current views on the localization of neural stem cells in the brain.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 139(2):77-9.
  • D E Korzhevskiĭ · E G Sukhorukova · O V Kirik · O S Alekseeva
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    ABSTRACT: Subventricular proliferative zone is the brain area most intriguing and least understood in terms of cellular and spatial organization. The objective of this study was to focus on the structural and cytochemical organization of astrocytes of the subventricular zone of the rat brain. Astrocytes were detected immunocytochemically. The preparations were analyzed using conventional light microscopy and confocal laser microscopy. The study performed showed that the major cellular population of the subventricular zone was represented by a special kind of atypical fibrous astrocytes with the intermediate filaments containing only glial fibrillary acidic protein (GFAP). The processes of these cells formed a complex double-layer network beneath the ependyma. The described structural pattern of the glial skeleton of the subventricular zone seems to be determined by specific functions of the area and its location in the zone of CSF-brain barrier.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 139(3):77-9. DOI:10.1007/s11055-012-9636-5
  • E I Chumasov · E S Petrova · D E Korzhevskiĭ
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    ABSTRACT: Using the immunohistochemical methods for detection of neurofilaments (NF), peripherin (PRF), synaptophysin (SF), tyrosine hydroxylase (TH) and Nissl-staining with toluidine blue, thick sections (10-20 microm) prepared through all parts of pancreas of adult Wistar rats (n=7) were studied. The topography and the density of distribution of pancreatic islets in the various parts of pancreas were defined. The greatest density of the islets was found in the body of pancreas. The dense innervation of the organ was detected which included several nervous plexuses: the big-looped one consisting of nervous bundles and small trunks of NF-positive myelinated and unmyelinated nervous fibers, the second one was formed by PRF-positive thin posganglionic bundles of axons and microganglia, and the third one, detected with SF-staining, was main terminal plexus consisting of varicose axons with en passant synapses. Interactions of synaptophysin-positive terminals (distant en passant synapses) with blood vessels, endocrine (islet) and exocrine cells, excretory ducts of the pancreatic lobules are described in details. Peculiarities of the structure of parasympathetic ganglia, their neurons, and pericellular synaptic apparatus are described; problems of the innervation and the nature of pancreatic insular endocrine cells are discussed. Attention is drawn to the fact that in no case neurons were found in the islets in the rat pancreas.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 139(3):51-8. DOI:10.1007/s11055-012-9635-6
  • D E Korzhevskiĭ · O V Kirik · A V Giliarov
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    ABSTRACT: Ezrin (cytovillin or p81 protein) is an actin-binding protein, a member of ERM (ezrin, radixin and moesin) family, which species contribute to stabilization of the plasma membrane-formed structures. The aim of the present study was to demonstrate the ezrin-containing cells in the rat brain and to describe their topography and morphological features. The most pronounced immunohistochemical reaction to ezrin was found in the epithelium of the choroid plexus, cells of the subcommissural organ and ventricular ependyma. Moreover, ezrin staining was also detected in the unidentifiable cells in the subventricular zone, rostral migration pathway and astrocytes in various brain areas. Preferential ezrin localization in the brain cells contributing to formation of barrier structures suggests its involvement in transport processes in the CNS.
    Morfologiia (Saint Petersburg, Russia) 01/2011; 140(6):86-8. DOI:10.1007/s11055-012-9674-z
  • D É Korzhevskiĭ · O V Kirik · E G Sukhorukova · A V Giliarov
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    ABSTRACT: Semi-conducting nanocrystals represent a new class of fluorescent inorganic objects which have a promising perspective for the application in biology and medicine. The aim of the current work was a determination of advantages and shortcimingss of quantum dots (QD) application in immunocytochemistry. It was shown that streptavidin-QD conjugates have more advantages (i.e. high intensity of fluorescence, photostability, wide excitation range, short and symmetric emission range) as compared to streptavidin conjugates with organic fluorochromes. This allows to recommend the use of QD in immunocytochemical studies. However, there are several disadvantages (like lower stability during long-term storage as compared to that one of organic fluorochrome conjugates, poor safety of aliquots, impossibility of long-term preservation of fluorescence of stained sections during their storage, incompatibility with several commercial mounting media) that limit the widespread application of nanocrystals in immunocytochemical studies.
    Morfologiia (Saint Petersburg, Russia) 01/2010; 137(3):71-5.
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    ABSTRACT: Morphological changes in the spinal cord of rats with different intensity of pathological symptoms were studied at the peak of the experimental encephalomyelitis development. Light-microscopical and immunohistochemical methods were used. Distribution of proliferating cell nuclear antigen (PCNA), astrocyte marker - glial fibrillar acidic protein (GFAP), and microglia and macrophage marker Iba-1, was studied. Heterogeneity in morphological manifestations of the experimental allergic encephalomyelitis was shown. Four typical patterns of morphological manifestations of the disease were demonstrated depending on the preferential involvement of pia mater, vessels, spinal cell nuclei or conductive tracts in the pathological process.
    Morfologiia (Saint Petersburg, Russia) 01/2010; 137(5):16-20.
  • A V Giliarov · O V Kirik · D É Korzhevskiĭ
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    ABSTRACT: In immunomorphological studies, it is often necessary to visualize several antigens simultaneously within the same histological sections. However in practice, the application of double staining is limited due to methodological complexity. The aim of the current study was to reproduce different methods recommended for multiple immunohistochemical staining of one section and to estimate their advantages and drawbacks. Six different methodological approaches for detection of several antigens in one slide were examined. It was found that the method providing the widest resources for analysis was the fluorescent detection, the most convenient was the application of ready-to-use standardized reagents, and the cheapest method was two-color reaction with DAB-type chromogen.
    Morfologiia (Saint Petersburg, Russia) 01/2010; 137(5):59-64.
  • E I Chumasov · D É Korzhevskiĭ · E S Petrova · N N Kuznetsova · N S Sapronov
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    ABSTRACT: The purpose of this study was to investigate the long-term reactive changes in the subventricular zone (SVZ) of the rat brain lateral ventricles after the intraventricular injection of beta-amyloid peptide (BAP). It was found that 3 months after BAP injection ependymal cells and SVZ cells reacted to the intraventricular BAP injection by the formation of glial structures, similar to amyloid plaques in their composition, cell structure and possible developmental mechanism. Using immunohistochemical methods, it was shown that they consisted mainly of reactive astrocytes and microgliocytes. It is suggested that atypical morphogenesis of these structures is associated with the interrelationship between the degenerative and regenerative processes, which involve both ependymal cells and forebrain proliferative zone progenitor cells.
    Morfologiia (Saint Petersburg, Russia) 01/2010; 137(6):24-8.
  • A N Vetosh · V B Kostkin · O A Alekseeva · D E Korzhevskiĭ
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    ABSTRACT: The spontaneous motor activity and pose reflexes of male adult rats (Wistar) were observed in the course of high pressure nitrogen compression up to 4,1 MPa. The experiments were carried out under normoxic and hypoxic conditions. Stabile rat motor cortex oxygen tension was recording during the nitrogen compression up to 7,1 MPa under normoxic condition. Sensitivity to nitrogen high pressure to be on the increase under hypoxic conditions. In its turn, resistibility to nitrogen high pressure to be on the decrease under hypoxic conditions (oxygen partial pressure from 0,012 to 0,004 MPa). Quantity of high dencity heat shock proteins (Hsp70) rats motor cortex neurons was 3,44 times higher after course of high pressure nitrogen compression up to 4,1 MPa. For hypoxic exposure (6% O2) the difference was less pronounced - 2,2 times. Data about rat motor cortex neurons Hsp70 concentration under high nitrogen pressure and low oxygen pressure may turn to be a clear base for explanation hypoxic influence on processes of nitrogen narcosis.
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    ABSTRACT: The aim of the present study was to demonstrate a neuronal form of NO-synthase (type I NOS) in rat brain using immunocytochemical technique in comparison with the histochemical reaction demonstrating NADPH-diaphorase activity, as well as finding of optimal fixation protocol for the NOS demonstration. The histochemical reaction product was found to have strict cytoplasmic distribution. Immunocytochemical visualization of nNOS revealed not only cytoplasmic, but also nuclear localization of nNOS in some part of neural and glial cells without cytoplasmic nNOS staining. Searching for an optimal protocol of the material fixation and the primary antibody dilution, we succeeded in diminishing the intensity of intranuclear nNOS-like reaction while the specific cytoplasmic nNOS reaction was retained. The results obtained show that the optimal demonstration of nNOS in paraffin sections requires immersion fixation in Carnoy solution or posfixation in this solution following a perfusion with 4% paraformaldehyde solution.
    Morfologiia (Saint Petersburg, Russia) 02/2007; 132(4):77-80.
  • D E Korzhevskiĭ · A V Giliarov
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    ABSTRACT: The purpose of the present study was to examine the corpora amylacea (amyloid bodies, polyglucosan bodies) in human brain. To test the hypothesis on their neuronal origin, distribution of the selective neuronal marker NeuN was studied immunocytochemically in the brain areas containing amyloid bodies. The studied neuronal marker NeuN was detected in most of the amyloid bodies. The data obtained confirm the neuronal origin of the amyloid bodies.
    Morfologiia (Saint Petersburg, Russia) 02/2007; 131(2):75-6.

Publication Stats

46 Citations
0.05 Total Impact Points


  • 1996–2012
    • Russian Academy of Medical Sciences
      • • Institute of Experimental Medicine, St.Petersburg
      • • NorthWest Branch
      Moskva, Moscow, Russia
  • 2002
    • D. O. Ott Research Institute of Obstetrics and Gynecology
      Sankt-Peterburg, St.-Petersburg, Russia
  • 1998
    • Saint Petersburg Medical Academy
      Sankt-Peterburg, St.-Petersburg, Russia