D E Korzhevskiĭ

Russian Academy of Medical Sciences, Moskva, Moscow, Russia

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Publications (75)0.05 Total impact

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    ABSTRACT: The regenerative capacity of the Central Nervous System (CNS) is a key factor implicated in the pathogenesis of neurodegenerative diseases. In the present study, the regenerative capacity of the CNS is considered using one of the markers of regeneration, Growth Associated Protein-43 (GAP-43) and its proteolytic fragment GAP-43-3 in the Experimental Autoimmune Encephalomyelitis (EAE) animal model of multiple sclerosis. The EAE on Wistar rats was characterized as an adequate model of multiple sclerosis, with typical clinical (pares and paralysis) and morphological (infiltration of spinal cord and deformation of motoneurons) disorders. Normally about 60% of GAP-43 is cleaved by m-calpain and stays in the form of GAP-43-3. During severe form of EAE up to 85% of GAP-43 can be found cleaved. We speculated that the cleavage of GAP-43 can play a crucial role for regenerative capacity of CNS during EAE development. Thus the distribution of GAP-43 and GAP-43-3 in the spinal cord was analyzed. The manifestation of clinical signs of EAE has been found to be in correlation with the levels of GAP-43 proteolysis both in the homogenate of the spinal cord and on the spinal cord slices. The immunoreactive staining enabled the observation of the accumulation of GAP-43-3 predominantly in microglial cells.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 04/2015; 101(1):74-84.
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    ABSTRACT: Neuroglobin is a recently discovered heme-containing protein located predominantly in the mammalian brain. This paper for the first time presents the data on neuroglobin distribution in human cerbellum using immunohistochemistry. Neuroglobin immunoreactivity in the cerebellum was found in all the cases studied (n = 7), although its intensity varied. Distinct reaction was found in Purkinje cells and the areas of cerebellar glomeruli.
    Morfologiia (Saint Petersburg, Russia) 01/2015; 146(4):75-7.
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    ABSTRACT: The purpose of this study was to develop the method for the simultaneous visualization of mast cells (MCs) and nerve terminals, based on generally accepted techniques of histochemical identification of MCs with alcian blue and immunohistochemical detection of synaptophysin. The protocol presented allows simultaneous identification of mast cells and nerve terminals in the sections of paraffin-embedded thymus of laboratory mammals with high selectivity and good reproducibility. The method can be used for both visualization of spatial relationship between MCs and nerve terminals and independent research of the innervation of mammalian internal organs. Zinc-ethanol-formaldehyde is recommended as an optimal fixative.
    Morfologiia (Saint Petersburg, Russia) 10/2014; 145(2):70-3.
  • O V Kirik · E G Sukhorukova · O S Alekseeva · D É Korzhevskiĭ
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    ABSTRACT: The goal of the study was to identify the subependymal microglial cells of the III ventricle of the rat brain and to determine their structural characteristics. The sections of the brain of intact Wistar (n = 3) and Sprague-Dawley (n = 3) male rats were studied using the methods of immunocytochemistry and confocal laser microscopy. Subependymal microglia of the III ventricle was found to be a constantly present cell population. Two types of subependymal microgliocytes were identified--spindle-like and basket cells. Their processes penetrate the ependymal layer and reach its surface, thus contacting the cerebrospinal fluid (CSF), which suggests a possible participation of these cells in the structure of CSF-brain barrier.
    Morfologiia (Saint Petersburg, Russia) 10/2014; 145(2):67-9.
  • D E Korzhevskiĭ · I P Grigor'ev · O V Kirik · N M Zelenkova · E G Sukhorukova
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    ABSTRACT: A protocol of immunocytochemical demonstration of choline acetyltransferase (ChAT), a key enzyme of acetylcholine synthesis, in paraffin sections of the brain of some laboratory animals, is presented. The method is simple, gives fairly reproducible results and allows for demonstration of ChAT in neurons, nerve fibers, and terminals in preparations of at least three species of laboratory animals including rat, rabbit, and cat. Different kinds of fixation (10% formalin, 4% paraformaldehyde, or zinc-ethanol-formaldehyde) were found suitable for immunocytochemical visualization of ChAT, however, optimal results were obtained with the application of zinc-ethanol-formaldehyde
    Morfologiia (Saint Petersburg, Russia) 04/2014; 144(6):69-72.
  • E A Kolos · D E Korzhevskiĭ
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    ABSTRACT: The aim of this study was to determine the possibility of immunohistochemical demonstration of various tissue antigens in paraffin sections of zinc-ethanol-formaldehyde-fixed spinal cord, surrounded by the vertebral tissues and adjacent muscles, after decalcification in formic acid, in adult (n = 5) and newborn (n = 2) Wistar rats. In the present work, the antibodies used were against: glial fibrillary acidic protein, neurofilaments, neuronal specific nuclear protein, enzyme tyrosine hydroxylase, protein of synaptic vesicles--synaptophysin and the intermediate filament protein--vimentin. Combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution was shown to provide an optimal preservation of the antigens studied.
    Morfologiia (Saint Petersburg, Russia) 03/2014; 144(4):76-9.
  • D E Korzhevskiĭ · O V Kirik · E G Gilerovich
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    ABSTRACT: Elaboration of the concept of neural stem cells at the end of the twentieth century resulted in the appearance of the multitude of new terms used for the designation of cells localized in the proliferative zones of the brain. Concurrently, a problem arose concerning the correlation of newly named cells, generally known elements of the nervous tissue, and classic views on its development. In the present study, an attempt is made on the basis of the literature data review and authors' own experience in the exploration of proliferative zones, to classify cell populations involved in the neurogenesis considering, primarily, the possibilities of their morphological and immunocytochemical in situ identification.
    Morfologiia (Saint Petersburg, Russia) 03/2014; 144(4):88-92.
  • E G Sukhorukova · I P Grigor'ev · O V Kirik · O S Alekseeva · D E Korzhevskiĭ
    Zhurnal evoliutsionnoĭ biokhimii i fiziologii 01/2014; 49(3):236-8.
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    ABSTRACT: The authors proposed a number of immunohistochemical markers to be used in histological diagnostic studies of the heart and protocols for their identification that extend the possibilities for the estimation of the heart function in case of pathological changes and sudden cardiac death.
    Sudebno-meditsinskaia ekspertiza 01/2014; 56(4):38-40.
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    ABSTRACT: The results of examination and surgical treatment of 298 patients with chronic pancreatitis and the original morphological investigations of material of the pancreas were studied. The data allowed the detection of additional criteria features of inclusion of the patients with chronic pancreatitis to be made in groups according to foreign Marseilles-Roman classification (1988). It is shown, that the basis of study of morphogenesis of chronic pancreatitis is immunohistochemical method, which let the authors diagnose not only the pathological changes of exo- and endocrine sections of pancreas, but at the same time the structural features of nervous apparatus and vessels of microcircular bed. The revealed morphological features of different forms of chronic pancreatitis vs clinical finding characteristics and the data of instrumental and laboratory methods of research allow the substantiation of surgical treatment version to be made.
    Vestnik khirurgii imeni I. I. Grekova 12/2013; 172(4):29-39.
  • E S Petrova · E N Isaeva · D E Korzhevskiĭ
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    ABSTRACT: The purpose of this paper was to examine the possibilities of engraftment, and to study the differentiation of the dissociated cells from the embryonic primordia of the spinal cord and the neocortex of Wistar rats, after their transplantation into the sciatic nerve of adult animals. The cell suspension obtained as a result of a dissociation of fragments of the cervical spinal cord and the anterior cerebral vesicle from rat fetuses at day 15 of development, was injected into the proximal segment of a previously damaged sciatic nerve. Using the immunocytochemichal marker of neural stem/progenitor cells (Msi-1) the transplanted cells were identified in the nerve trunks after 1 day after the operation. After 21 day some of these cells underwent differentiation into NeuN-immunopositive neurons, however their number was small. Thus, dissociated precursor cells from embryonic rat spinal cord and neocortex survive for three weeks under conditions of transplantation into the damaged nerve and retain the ability to differentiate into neurons, but the number is small. Most of the cells in the neocortex transplants, unlike those from spinal cord transplants, within 21 days after the operation were represented by the ependymocytes.
    Morfologiia (Saint Petersburg, Russia) 07/2013; 143(2):30-4.
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    ABSTRACT: The paper presents an analysis of authors' results obtained using fixation of various tissues in zink-ethanol-formaldehyde (ZEF). It was found that fixation in ZEF, in comparison with other methods of fixation, allowed to achieve higher sensitivity of immunocytochemical reaction for a large number of antigens studied and, in many cases, to avoid heat unmasking of antigens. It also provided high resolution of the images obtained using the fluorescent and confocal laser microscopy. However, the studies of antigens with small molecular mass revealed the antigen diffusion from the site of original localization. The data obtained suggest that fixation of a material in ZEF fixative is promising for both immunocytochemical studies, including those using the fluorescent and confocal laser microscopy, and general histological practice.
    Morfologiia (Saint Petersburg, Russia) 07/2013; 143(2):81-5.
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    ABSTRACT: The aim of the study was to investigate neuroprotective effect of creatine glycine ethylic ether fumarate (creamide). The methods involved intragastric administration of creamide in doses of 30 and 50 mg/kg twice a day for 10 days. Focal 30 minutes cerebral ischemia model by endovascular suture occlusion of the middle cerebral artery in a rat with subsequent reperfusion period for 48 hours was produced. Assessment of creamide stability in gastric juice was performed. Ischemic lesion volume accompanying focal ischemia was visualized and determined. Similar infarction patterns had been found with histological methods. Garcia scale was used for clinical study of neurological deficit in rats. Our data suggest a significant neuroprotective effect of creamide in dosage 50 mg/kg administered twice a day which decreased brain lesion volume produced by ischemic and reperfusion injury.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2013; 98(10):1258-63.
  • D E Korzhevskiĭ · M V Lentsman · O V Kirik · V A Otellin
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    ABSTRACT: The aim of the study was to evaluate, by using immunohistochemistry (demonstration of Iba-1 protein), the morphological features of the rat hippocampal microglia and to describe its cellular types during the response to a single total brain ischemia of 12 min duration. The results obtained suggest the existence of several morphologic types of microgliocytes in the intact hippocampus, while some more types appear in response to ischemic lesion of neurons. These changes in general population of the hippocampal microgliocytes is related to their functional activation. During the post-ischemic period (3, 7 and 14 days following the challenge), there appeared multiple micro-outgrowths of the microglial cell processes, which suggests the enhancement of their interaction with surrounding cellular elements and may be the manifestation of the process of the elimination of neuropil lesioned synaptic structures.
    Morfologiia (Saint Petersburg, Russia) 01/2013; 142(5):30-3.
  • E G Sukhorukova · M S Zakhriapin · N M Anichkov · D E Korzhevskiĭ
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    ABSTRACT: Relatively simple protocol for identification of microgliocytes in archived material is proposed. A novel variant is based on preparation processing with the use of polyclonal antibodies against Iba-1 protein. The proposed approach includes antigen heat unmasking in a modified citrate buffer and prolonged incubation of the sections with the primary antibodies at high temperature. The method is highly specific and allows selective detection of microgliocytes in sections prepared from a material stored in formalin for 2 months.
    Morfologiia (Saint Petersburg, Russia) 01/2013; 142(5):68-71.
  • D E Korzhevskiĭ · E G Sukhorukova · I P Grigor'ev
    Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova / Ministerstvo zdravookhraneniia i meditsinskoi promyshlennosti Rossiiskoi Federatsii, Vserossiiskoe obshchestvo nevrologov [i] Vserossiiskoe obshchestvo psikhiatrov 01/2013; 113(6):77-80. · 0.05 Impact Factor
  • E I Chumasov · P A Voronchikhin · D É Korzhevskiĭ
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    ABSTRACT: In this investigation the peculiarities of innervation of bronchi and blood vessels of the lung were studied in 20 rats using immunohistochemical demonstration of synaptophysin and alpha-actin. The results obtained have showen that the densest innervation is typical for bronchial walls, particularly, for the muscular lamina. Synaptophysin-immunoreactive terminals (SFIT) were detected in the bronchi in close association with both circular bundles of smooth muscle cells and microganglia. Dense network of SFIT was found in the pulmonary vein--in its middle tunic formed by cardiomyocytes. In contrast to the bronchi and pulmonary vein, large branches of the pulmonary artery contained no SFIT. We briefly discuss the problem of the origin of the nerve fibers described and their functions and suggest that SFIT are formed by efferent fibers (axons) of neurons arising from either the intrapulmonary parasympathetic ganglia.
    Morfologiia (Saint Petersburg, Russia) 12/2012; 142(4):49-53.
  • O V Kirik · T D Vlasov · D É Korzhevskiĭ
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    ABSTRACT: Nestin and Musashil (Msi-1) proteins are most often used for labeling of neural stem cells and progenitor cells in vivo, however it remains unclear if these markers really label the same cells. As a result of the study of structural characteristics and localization of nestin- and Msil-expressing cells it was found that these proteins were detected in non-identical cell populations in the brain of intact 15 rats. We failed to find cell groups demonstrating a coexpression of nestin and Msi-1. However, after ischemic lesion of the brain, which was caused in 38 rats by an endovascular occlusion of the left medial cerebral artery for 30 min with the following reperfusion for 48 hours, both markers were detected in cells of subventricular zone and in ependymocytes. These results indicate the changes in cytochemical patterns of the candidate stem cells.
    Morfologiia (Saint Petersburg, Russia) 12/2012; 142(4):19-24.
  • I P Grigor'ev · E G Sukhorukova · E A Kolos · D E Korzhevskiĭ
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    ABSTRACT: The distribution of tyrosine hydroxylase (TH), a key enzyme of catecholamine synthesis, was studied immunocytochemically in the neurons of substantia nigra in the human brain (n=7), and localization of neuromelanin in these cells was determined. The evidence indicating the existence of three types of neurons in substantia nigra was obtained, including the neurons containing both TH and neuromelanin, neurons containing only neuromelanin, and neurons expressing neither TH, nor neuromelanin. Presence of a population of neuromelanin-containing neurons lacking TH (which make up 7-30% of the cells) is discussed considering the participation of catecholamines and TH in neuromelanin synthesis.
    Morfologiia (Saint Petersburg, Russia) 08/2012; 141(2):65-7. DOI:10.1007/s11055-013-9755-7
  • D E Korzhevskiĭ · O V Kirik · E G Sukhorukova · T D Vlasov
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    ABSTRACT: Microgliocytes are currently known as structurally labile cells, that, by changing the length of their processes, control the synaptic organization of the brain. The aim of this study was to investigate structural organization of microglia of the striatum and of the forebrain periventricular area in rat following transitory focal ischemia. In the absence of brain infarction, ischemic lesion was found to activate microglia, which contributed to rearrangement of striatal neuropil. As microgliocytes became activated, they changed their shape from characteristic dendritic one to oval and rounded, which are typical to amoeboid microglia. Microgliocytes were shown to be capable of proliferation.
    Morfologiia (Saint Petersburg, Russia) 08/2012; 141(2):28-32.

Publication Stats

47 Citations
0.05 Total Impact Points


  • 1996–2012
    • Russian Academy of Medical Sciences
      • • Institute of Experimental Medicine, St.Petersburg
      • • NorthWest Branch
      Moskva, Moscow, Russia
  • 2002
    • D. O. Ott Research Institute of Obstetrics and Gynecology
      Sankt-Peterburg, St.-Petersburg, Russia
  • 1998
    • Saint Petersburg Medical Academy
      Sankt-Peterburg, St.-Petersburg, Russia