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C W Lee
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ABSTRACT: Autoantibodies that are characteristic of autoimmune bullous diseases (AIBDs) can be detected by immunoblot assay using epidermal or dermal extracts. However, none of the substrates obtained by ordinary methods seems to contain a sufficient amount of all of the autoantigens involved in AIBDs, and diagnosis may require the use of several different substrates.
To examine the potential of A431 cell extracts as a substrate for immunoblotting.
Fourteen sera obtained from patients with major AIBDs (pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, bullous pemphigoid and epidermolysis bullosa acquisita) were tested using this substrate.
Bands corresponding to desmoglein 1 and 3, desmoplakin 1 and 2, periplakin, BPAG1, BPAG2 and type VII collagen were identified distinctly with these sera.
This finding suggests that culture extracts of A431 cells provide an effective substrate for the diagnosis and differential diagnosis of major AIBDs.
British Journal of Dermatology 11/2000; 143(4):821-3. · 3.67 Impact Factor
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C W Lee
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ABSTRACT: Annular erythema (AE) associated with anti-Ro (SSA) and/or La (SSB) autoantibody in patients with Sjögren syndrome (SS) or with SS/systemic lupus erythematosus overlap syndrome (SS/SLE), has recently been described in Orientals, and it may be a counterpart of annular skin lesion of the subacute cutaneous LE seen mostly in Caucasians. The author examined five Korean AE patients in respect to dinical diversity. In this small-sample study, subtle differences appeared between individual cases regarding the serologic features and the diagnoses of the disease. Among the five cases, four had circulating anti-Ro and anti-La antibodies, and one had only anti-La. Regarding the diagnosis, one was SS/SLE, two were primary SS, and the remaining two were only "AE associated with anti-Ro/La antibody". There seem to be a wide clinical spectrum in the disease expression of AE associated with anti-Ro/La autoantibody than previously thought.
Journal of Korean Medical Science 05/2000; 15(2):199-202. · 0.99 Impact Factor
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ABSTRACT: Studies at the population level have demonstrated associations between pemphigus (pemphigus vulgaris, PV, and pemphigus foliaceus, PF) and particular HLA haplotypes, which suggests that there may be a genetic predisposition for the disease.
The aim of the present study was to examine the distribution/frequency pattern of HLA class II alleles (DRB1, DQA1 and DQB1) from a group of 30 Korean patients with pemphigus (15 PV and 15 PF) by PCR amplification with sequence-specific primers.
In PV, the frequency of DRB1*01 allele was found to be significantly high (pc = 0.0014); in PF, DRB1*01, DQA1*0302 and DQB1*0603 alleles showed positive associations with statistical significances (pc = 0.0002, 0.0007 and 0.0067, respectively), when compared with those found in Korean controls.
In this small-sample study, findings of allelic frequencies among Korean patients with pemphigus are somewhat different from those found in other populations.
Dermatology 02/1998; 197(4):349-52. · 2.05 Impact Factor
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ABSTRACT: The distribution pattern of HLA class II alleles in 12 Korean patients with epidermolysis bullosa acquisita was examined. The major difference in the allelic frequency comparing with the controls was a higher DRB1*13 allele with these patients (p = 0.066).
Dermatology 02/1996; 193(4):328-9. · 2.05 Impact Factor
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ABSTRACT: A 44-year-old woman presented with blisters on her face of 1-month's duration. A biopsy showed a subepidermal bulla with a dermal infiltrate of inflammatory cells and thick linear deposits of immunoreactants along the dermo-epidermal junction. Using Western immunoblots, the patient's IgG antibody was found to recognize type VII procollagen. Moderate doses of oral prednisolone resulted in a complete remission, without a recurrence for more than 3 years.
Clinical and Experimental Dermatology 10/1992; 17(5):363-5. · 1.20 Impact Factor
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Journal of the American Academy of Dermatology 08/1991; 25(1 Pt 1):110-1. · 3.99 Impact Factor
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ABSTRACT: We report two infants, aged 4 and 9 months, respectively, who had recurrent cutaneous lesions of Langerhans cell histiocytosis confirmed by immunohistochemical and ultrastructural studies. The lesions were confined to the skin and appeared as transient papules on the trunk in both patients. Since neither showed evidence of internal organ involvement or further recurrences of the skin lesions during follow-up periods of 22 and 24 months, respectively, no therapy was given. These cases may be considered as an uncommon benign variant of the Langerhans cell proliferative disorder.
British Journal of Dermatology 09/1988; 119(2):259-65. · 3.67 Impact Factor
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C W Lee
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ABSTRACT: A 55-year-old woman with epidermolysis bullosa acquisita (EBA) who had associated vesicular cystitis is described. The clinical, histological and immunohistological features of the skin and mucous membranes were in accordance with previous reports of this disease. The patient also had symptoms of cystitis, and a cystoscopic examination showed areas of erosions and vesicles in the wall of the urinary bladder. Histological and immunopathological studies of the urinary bladder demonstrated sub-mucosal vesicles, and linear deposits of IgG and C3 along the mucosal-lamina propria junction. These bladder changes may be induced by the autoantibodies present in EBA.
British Journal of Dermatology 08/1988; 119(1):101-5. · 3.67 Impact Factor
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Journal of the American Academy of Dermatology 04/1988; 18(3):579-81. · 3.99 Impact Factor
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ABSTRACT: A 43-year-old man with generalized lichen planus demonstrated serum antibodies against autologous lesional skin. Indirect immunofluorescence using serum and papular lesional skin revealed a lichen planus specific antigen found only in the granular layer. The specific tissue antigen was not detected in normal skin from this patient, in normal skin from patients with skin disorders other than lichen planus or in skin from normal control persons. When titers of the serum antibodies against lichen planus antigen were examined before and after a successful therapy a positive correlation of the titer could be found in this patient.
Journal of Korean Medical Science 01/1988; 2(4):259-62. · 0.99 Impact Factor
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ABSTRACT: A 45-year-old woman with epidermolysis bullosa aquisita is presented. The clinical, histological, and immunopathological features were in keeping with the previous reports of this disease. The patient also had anti-basal cell cytoplasmic antibodies at a significant titer, which is considered an unusual finding associated with this disorder. Treatment with a moderate dose of corticosteroid was effective in controlling the bullous lesions.
Journal of Korean Medical Science 10/1986; 1(1):25-9. · 0.99 Impact Factor
