C Pathirana

Publications (3)4.01 Total impact

• Article: Effect of Asparagus falcatus on Acetaminophen Toxicity in Mice: A Comparison of Antioxidative Effect With N-Acetyl Cysteine.

  R P Hewawasam, K A P W Jayatilaka, C Pathirana
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  ABSTRACT: Objective: In this study, the effect of Asparagus falcatus extract on acetaminophen-induced liver injury was investigated in vivo. Method: Six arms of study. ICR mice (n = 20) were treated with acetaminophen at a single dose of 300 mg/kg (in saline, after a 16-hr fast) to induce hepatotoxicity. Drug control group and pre- and posttreated groups were administered 0.9 g/kg of Asparagus falcatus orally. Serum ALT, AST, ALP, liver GSH, antioxidant enzymes, GPx (glutathione peroxidase), GR (glutathione reductase), GST (glutathione-S-transferase), and liver/serum malondialdehyde (MDA) concentrations were estimated. Liver damage was also assessed histopathologically. The effect of the plant extract was compared with N-acetyl cysteine. Results: Acetaminophen produced liver damage, as manifested by a significant rise (P <. 001, one-way ANOVA) in serum ALT, AST, and ALP, and a reduction (P <. 001) in the liver reduced glutathione (GSH) as compared to respective controls. All enzyme activities and liver GSH were significantly improved in Asparagus-treated mice, with pretreatment providing better results than posttreatment (P <. 05). Histopathologically, mice pretreated with Asparagus showed no liver necrosis. A significant improvement was observed in antioxidant enzyme activities of GPx, GR, and GST in the Asparagus pretreated group (P <. 05). Mice posttreated with Asparagus showed a significant reduction in MDA formation (P <. 05). Conclusion: These results suggest that the feeding regimen with Asparagus extract inhibited the progression of hepatic injury induced by acetaminophen.
  Journal of Dietary Supplements 01/2008; 5(1):1-19.
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  Available from: icmr.nic.in

  Article: Hepatoprotective effect of Epaltes divaricata extract on carbon tetrachloride induced hepatotoxicity in mice.

  R P Hewawasam, K A P W Jayatilaka, C Pathirana, L K B Mudduwa
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  ABSTRACT: Epaltes divaricata is widely used in Sri Lanka as an Ayurvedic medicine. In the present study the hepatoprotective and antioxidative effects of an aqueous extract of E. divaricata plant (Family-Compositae) were investigated against carbon tetrachloride induced hepatocellular injury in mice. Healthy male mice (30-35 g body weight, 6-8 wk old) were used. A single dose of carbon tetrachloride (CCl4, 0.5 ml/kg in olive oil) was administered ip to induce hepatotoxicity and the plant extract at a dose of 0.9 g/kg was administered orally by gavage. Animals were sacrificed 24 h and 4 days after the administration of CCl4. Blood and liver tissue were collected for the assessment of serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and liver reduced glutathione level. The liver tissue was used for histopathological assessment of liver damage. Pre-treatment of mice with the plant extract of Epaltes (0.9 g/kg) orally for 7 days significantly reduced serum levels of ALT (P<0.01), AST (P<0.01) and ALP (P<0.001) enzymes by 21.40, 47.36 and 71.12 per cent respectively and significantly increased (P<0.001) the liver reduced glutathione level by 42.32 per cent, 24 h after the administration of carbon tetrachloride. A marked improvement in the enzyme activities and the liver reduced glutathione level was observed in the Epaltes pre-treated mice 4 days after the administration of carbon tetrachloride. Histopathological studies provided supportive evidence for the biochemical analysis. The results of the present study indicated that under the present experimental conditions, aqueous extract of Epaltes divaricata showed hepatoprotective abilities against carbon tetrachloride induced liver damage in mice.
  The Indian journal of medical research 07/2004; 120(1):30-4. · 1.84 Impact Factor
• Article: Protective effect of Asteracantha longifolia extract in mouse liver injury induced by carbon tetrachloride and paracetamol.

  R P Hewawasam, K A P W Jayatilaka, C Pathirana, L K B Mudduwa
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  ABSTRACT: This study was conducted to investigate the protective effect of Asteracantha longifolia Linn (Acanthaceae) plant extract on carbon tetrachloride (CCl4)- and paracetamol-induced acute hepatotoxicity in mice. Hepatotoxicity was induced by the administration of a single intraperitoneal dose of CCl4 (0.5 mL kg(-1) CCl4 in olive oil) in one model and in the other by administration of paracetamol (300 mg kg(-1) in saline) orally, after a 16-h fast. An aqueous extract of the whole plant (0.9 g kg(-1)) was used on a pre- and post-treatment basis. Asteracantha reduced the alanine aminotransferase (ALT) level by 69.32% (P < 0.001) and increased the liver reduced glutathione level by 64.65% (P < 0.001) in the pre-treated group, 4 days after the administration of CCl4. A similar pattern was observed in the pre-treated group 4 h after the administration of paracetamol with a reduction in serum levels of ALT, aspartate aminotransferase and alkaline phosphatase enzymes by 65.04, 55.79 and 45.75% respectively (P < 0.001). Plant extract also increased the glutathione concentration of the liver significantly (P < 0.001). Histopathological studies also provided supportive evidence for results from the biochemical analysis with marked improvement in liver architecture being observed in the Asteracantha-treated groups. Pre-treatment showed better results than post-treatment in both hepatotoxic models. Overall results indicate that the aqueous extract of Asteracantha longifolia possesses hepatoprotective effects on CCl4- and paracetamol-induced hepatotoxicity in mice.
  Journal of Pharmacy and Pharmacology 10/2003; 55(10):1413-8. · 2.17 Impact Factor