ABSTRACT: A computer program has been written for the kinetic method of substrate reaction (MSR) in enzyme modification studies. The program analyses a series of progress curves that continuously record signals (absorbance, fluorescence emission, etc.) reflecting the concentration changes of substrate or product during enzyme modification in the presence of substrate. After the apparent kinetic parameters are calculated by graphdrawing or curve-fitting method, a series of relevant plots is shown, from which information about the enzyme-inhibitor interaction can be deduced and microscopic kinetic constants calculated.
Computers in Biology and Medicine 08/1993; 23(4):301-6. · 1.09 Impact Factor