Byung Chul Jee

Seoul National University Bundang Hospital, Sŏul, Seoul, South Korea

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Publications (169)363.42 Total impact

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    ABSTRACT: To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. In this experimental study, we researched a total of 182 ovaries from 4-week-old ICR mice. The equilibration solution included 20% ethylene glycol (EG), and the vitrification solution included 40% EG, 18% Ficoll, and 0.3 M sucrose. Intact ovaries were first suspended in 1 mL of equilibration solution for 10 min, and then mixed with 0.5 mL of vitrification solution for 5 min. Ovaries were randomly assigned to 3 groups and 0, 5, or 20 mg/mL of type III AFP was added into the vitrification solution (control, AFP5, and AFP20 groups, respectively). The vitrified ovaries were evaluated after warming and 2 weeks after autotransplantation. The main outcome measurements are follicular morphology and apoptosis assessed by histology and the TUNEL assay. A significantly higher intact follicle ratio was shown in the AFP treated groups (control, 28.9%; AFP5, 42.3%; and AFP20, 44.7%). The rate of apoptotic follicles was significantly lower in the AFP treated groups (control, 26.6%; AFP5, 18.7%; and AFP20, 12.6%). After transplantation of the vitrified-warmed ovaries, a significantly higher intact follicle ratio was shown in the AFP20 group. The rate of apoptotic follicles was similar among the groups. The results of the present study suggest that supplementing AFP in the vitrification solution has beneficial effects on the survival of ovarian tissue during cryopreservation and transplantation.
    Yonsei medical journal 05/2015; 56(3):778-84. DOI:10.3349/ymj.2015.56.3.778 · 1.26 Impact Factor
  • Seul Ki Kim, Byung Chul Jee, Seok Hyun Kim
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    ABSTRACT: To assess global methylation and acetylation level before and after swim-up in ejaculated human sperm. Prospective experimental study. University-based hospital. Semen samples were obtained from 50 healthy volunteers (single sample per person) between December 2012 and May 2014. The semen samples were processed by the swim-up method. Sperm motility, strict morphology, DNA fragmentation index (DFI) by TUNEL, abnormal chromatin structure by toluidine blue staining, immunostaining of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as an oxidative stress marker, immunostaining of 5-methyl-cytosine (5-mC) as a measurement of methylation level and immunostaining of six acetylation markers (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) before and after swim-up. After swim-up, motility and the percentage of normal form was significantly elevated. In the swim-up fraction, DFI and the percentage of abnormal chromatin structure were significantly lower and 8-OHdG levels were significantly higher. The 5-mC levels were significantly lower in the swim-up fraction, whereas no changes were observed in the positivity of six acetylation markers. Elevated percentage of normal form and decreased DFI and 5-mC levels were observed only in nonsmokers; 8-OHdG level was significantly higher in the swim-up fraction only from smokers. After swim-up, 5-mC levels were positively correlated with DFI. This is the first study demonstrating decreased methylation and unchanged acetylation patterns in human sperm after swim-up preparation. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
    Fertility and sterility 04/2015; DOI:10.1016/j.fertnstert.2015.03.007 · 4.30 Impact Factor
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    ABSTRACT: The aim of this study was to identify the risk factors of twin gestation in fresh double day-3 embryo transfer cycles. Out of a total of 386 cycles of fresh double embryo transfers on day 3 between 2006 and 2013 at a single center, 72 women with single intrauterine gestational sac (GS) (single-GS group) and 32 women with double GS (double-GS group) were identified. The patients' clinical characteristics and laboratory results were extracted from electronic medical records and were compared. The double-GS group had a significantly younger age (P = 0.012), and higher bodyweight (P = 0.033) compared with the single-GS group. The total number of blastomeres (P = 0.013) and cumulative embryo score (P = 0.012) were higher in the double-GS group. By receiver-operator curve analysis, maternal age ≤ 35 years, maternal bodyweight > 60 kg, total number of blastomeres > 14, and cumulative embryo score > 49 were the cut-off values to predict twin pregnancy. Among eight women with all four risk factors, the twin pregnancy rate was 87.5%. There was no twin pregnancy among seven women with no risk factors. Younger age, higher bodyweight, and better embryo quality are all associated with a higher incidence of twin pregnancy after double embryo transfer on day 3. Single embryo transfer might be advisable for couples with risk factors for twin pregnancy. © 2015 The Authors. Journal of Obstetrics and Gynaecology Research © 2015 Japan Society of Obstetrics and Gynecology.
    Journal of Obstetrics and Gynaecology Research 04/2015; DOI:10.1111/jog.12687 · 0.93 Impact Factor
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    ABSTRACT: This study attempted to derive an objective and sophisticated definition of poor ovarian response (POR). A total of 176 consecutive in vitro fertilization (IVF) cycles (137 patients) with conventional ovarian stimulation during 2009 to 2012 were studied by retrospective analysis. Optimal oocyte number (total or mature) was determined by statistics-based (distribution of oocyte number) and prognosis-based approaches (prediction for IVF outcome). Receiver operating characteristics curve analysis was used to show what number of oocytes could predict IVF pregnancy and whether clinical and laboratory variables could predict newly defined POR. The 25th percentile of the distribution corresponded to total oocytes ≤2 and mature oocyte ≤1. The cut-off values for the prediction of IVF outcomes were total oocytes >5 and mature oocyte >1. Considering the incidence of POR (34.1%), a reasonable definition of POR was decided as total oocytes ≤2 or mature oocyte ≤1. For the prediction of this new definition, the extreme cut-off value (by setting a false positive rate of 5%) of serum anti-Mullerian hormone (AMH) was ≤0.76 ng/mL, which was better than serum follicle stimulating hormone or age. A new simple definition of POR was derived as total oocytes ≤2 or mature oocyte ≤1 in a previous cycle or a serum AMH level of ≤0.76 ng/mL. When this simple criterion was re-applied to our data, the predictive performance was similar to the Bologna criteria. We here propose a new definition of POR, which is simple and supported by statistical and prognostic analyses.
    Yonsei Medical Journal 03/2015; 56(2):482-9. DOI:10.3349/ymj.2015.56.2.482 · 1.26 Impact Factor
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    ABSTRACT: This study aimed to evaluate the efficacy of random-start controlled ovarian stimulation (COS) in cancer patients for emergency fertility preservation. In this retrospective comparative study, 22 patients diagnosed with cancer and 44 infertile women undergoing conventional in vitro fertilization (IVF) were included. In cancer patients, ovarian stimulation was started on the day of referral, irrespective of their menstrual cycle date. The control group was selected by age matching among women undergoing conventional IVF. COS outcomes were compared between groups. The number of total and mature oocytes retrieved and the oocyte maturity rate were higher in the random-start group than in the conventional-start group. However, duration of ovarian stimulation was longer in the random-start group (11.4 vs. 10.3 days, P = 0.004). The addition of letrozole to lower the estradiol level during COS did not adversely affect total oocytes retrieved. However, oocyte maturity rate was lower in cycles with letrozole than in cycles without letrozole (71.6% vs. 58.2%, P = 0.019). Our study confirms the feasibility and effectiveness of random-start COS in cancer patients. Graphical Abstract
    Journal of Korean Medical Science 03/2015; 30(3):290-5. DOI:10.3346/jkms.2015.30.3.290 · 1.25 Impact Factor
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    ABSTRACT: To report the various anatomic locations of surgically and pathologically proven endometriosis. Pathologic reports (n=1,376) of women who underwent surgeries at a single center between April 2005 and March 2013 were retrieved from the electronic medical record system of the hospital. Pathologic reports were included after performing a search by using the key-words "endometrial cyst," "endometriotic cyst," "endometriosis," or "endometrioma." Only reports dealing with female patients were selected, and the pathologic reports of 1,350 women (1,374 surgery cases) were included in the analysis. The predominant location of endometriosis was the ovaries (96.4%), followed by the soft tissue (2.8%), gastrointestinal tract (0.3%) and urinary tract (0.2%). Laparoscopic surgery was the major surgical technique used for the pelvic endometriosis (89.2%). Adrenal gland endometriosis was found in a 55-year-old woman. We established the various anatomic locations of surgically and pathologically proven endometriosis in Korean women.
    01/2015; 58(1):53-8. DOI:10.5468/ogs.2015.58.1.53
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    ABSTRACT: To evaluate the association between the severity of endometriosis and the preoperative neutrophil-to-lymphocyte ratio (NLR) and serum level of cancer antigen 125 (CA 125). Data were obtained from the medical records of 419 patients who underwent laparoscopic conservative surgery for ovarian endometrioma between April 2005 and March 2013. Each patient's preoperative complete blood count was recorded and the endometriosis score was assessed. The endometriosis score was not associated with either the NLR or the serum level of CA 125. The endometriosis score was negatively related to preoperative hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. The only positive association was between NLR and the patients' age. NLR and preoperative serum anti-Müllerian hormone level were found to be negatively related. The severity of endometriosis was not associated with the serum level of CA 125 or the NLR. The presence of a negative correlation between the severity of endometriosis and red blood cell dynamics needs further investigation.
    12/2014; 41(4):151-7. DOI:10.5653/cerm.2014.41.4.151
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    ABSTRACT: Many studies have shown that ischemic injuries during the transplantation process were more detrimental than cryoinjuries for follicle survival and death, and it has been reported that transplantation sites can affect the outcomes of grafted ovarian tissue (OT). The purpose of this study was to assess the impact of different OT transplantation sites on follicular integrity and function of OT grafts. B6D2F1 mice were randomly assigned to control (sham) and four experimental groups according to transplantation sites (back muscle [BM], fat pad [FP], kidney capsule [KC], and subcutaneous [SC]). The ovaries from four groups were autotransplanted to each site. The OT recovery ratios on Days 2, 7, and 21 were significantly decreased in the FP group. The mean numbers of follicles were significantly lower in all the grafting groups compared with the sham group, except in the KC group on Days 7 and 21 and the BM group on Day 21. On Day 2, all the experimental groups showed low intact (G1) follicle ratio when compared with the sham group; however, the BM, KC, and FP groups recovered their morphologic integrity on Day 7, and only the SC group presented a significant decrease in G1 follicle ratios. On Day 21, the G1 follicle ratios of the FP and KC groups were greater than the sham control group. The proportion of apoptotic follicles of the four OT graft groups was higher than in the sham group on Day 2, followed by a significant decrease in the KC group and an increase in the SC group on Day 7. The serum follicle-stimulating hormone levels were significantly increased in all grafting groups on Day 2. On Day 7, only the SC group showed the high follicle-stimulating hormone level compared with the other groups. The mean numbers of oocytes from OT grafts were the highest in the KC group, except in the control group, and the lowest in the SC group. The ratios of mature oocytes were also significantly greater in the sham and KC groups. However, the ratios of normal spindle did not differ among the five groups. In conclusion, the KC was the optimal site for OT transplantation in this murine model, whereas the SC site was unfavorable for this procedure. In this study, we confirmed that the different grafting sites influenced the outcomes of transplantation. Copyright © 2014 Elsevier Inc. All rights reserved.
    Theriogenology 11/2014; 83(5). DOI:10.1016/j.theriogenology.2014.11.026 · 1.85 Impact Factor
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    ABSTRACT: Necrostatin 1 (Nec1) is widely used in disease models to examine the contribution of receptor-interacting protein kinase 1 in cell death. The biological actions of Nec1 are blocking necrotic cell death. The purpose of this study was to investigate whether adding Nec1 into in vitro maturation (IVM) media, followed by vitrification procedures, could enhance the survival and developmental competency of oocytes. Germinal vesicle oocytes were matured in IVM medium containing 2 different doses of Nec1 (0.5 and 1 μmol/L). After IVM, the oocytes were vitrified using a 2-step exposure to equilibrium and vitrification solutions. After warming, the rates of survival, fertilization, embryonic development up to blastocyst in vitro, morphology of spindle and chromosome, membrane integrity, mitochondria integrity, and several gene expressions were evaluated. The survival and developmental competency of oocytes were higher in the 1 μmol/L Nec1-treated group than control. The proportion with intact spindles/chromosomes and stable membranes was similar in all the groups. The mitochondrial integrity of all Nec1-treated groups showed a higher score with strong staining. The 1 μmol/L Nec1 showed significantly increased expressions of Mad2, Gdf9, and Bcl2. The Cirp level had a tendency to be downregulated in the 0.5 µmol/L Nec1 but upregulated in the 1 μmol/L Nec1, compared with the control. The Mtgenome expressions were significantly decreased in both Nec1 groups. The supplementation of 1 μmol/L Nec1 into the IVM medium could be beneficial for the survival and development of immature oocytes after vitrification.
    Reproductive sciences (Thousand Oaks, Calif.) 11/2014; DOI:10.1177/1933719114556482 · 2.18 Impact Factor
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    ABSTRACT: Objective To study variations in semen parameters among cancer patients who visited a sperm banking clinic before undergoing cancer treatment. Design Retrospective, consecutive study. Setting University-based hospital. Patient(s) Eighty-six patients, diagnosed with various cancers, undergoing multiple semen collections on 5 consecutive days, for fertility preservation, between 2004 and 2013. Intervention(s) None. Main Outcome Measures Within- and between-subject coefficients of variation were estimated using a random-effects analysis of variance to assess the consistency of semen parameters (volume, sperm concentration, motility, rapid motility, total motile sperm count, and computer-based sperm parameters), whereas intraclass correlation coefficients (ICCs) were calculated to assess the size of the between-component of variance relative to the total component of variance. Result(s) When analyzing semen parameters over a maximum of 5 consecutive days, only the semen volume was significantly reduced in day-1 and -3 samples compared with the first sample. Almost all of the parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were noted in volume (ICC 0.76; 95% confidence interval [CI] 0.52–0.89), followed by total motile count (ICC 0.71; 95% CI 0.30–0.89); the least consistent measure was wobble (ICC 0.14; 95% CI −0.13, 0.51). Conclusion(s) Repeated ejaculates from cancer patients did not show substantial variation in semen quality.
    Fertility and Sterility 10/2014; 102(4). DOI:10.1016/j.fertnstert.2014.06.045 · 4.30 Impact Factor
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    Yeo Joo Kim, Jina Youm, Jee Hyun Kim, Byung Chul Jee
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    ABSTRACT: ObjectiveTo investigate the incidence of actinomyces-like organisms in routine cervical smears of Korean women and to evaluate its association with the development of pelvic inflammatory disease (PID) in these women.MethodsThe results of cervical smears between 2011 and 2012 at a single university hospital were searched. If positive for actinomyces-like organisms, the medical record of the patient was searched and development of PID was followed. If the data were not available in the medical record, the patient was contacted by telephone.ResultsThe incidence of actinomyces-like organisms in cervical smears was 0.26% (52/20,390). Forty-two women (80.8%) were intrauterine device (IUD) users: the copper-IUD in 25 women and the levonorgestrel-releasing intrauterine system in 13 women (type unknown in 4 women). The majority (71.4%, 30/42) of the IUD users maintained the IUD. Prophylactic antibiotics were prescribed to 12 women. Two continuous IUD users were later diagnosed with PID, but these cases were not pelvic actinomycosis.ConclusionIt would be a reasonable option to choose the expectant management for asymptomatic women who incidentally showed actinomyces-like organisms in their cervical smear.
    09/2014; 57(5):393-6. DOI:10.5468/ogs.2014.57.5.393
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    Fertility and Sterility 09/2014; 102(3):e155-e156. DOI:10.1016/j.fertnstert.2014.07.535 · 4.30 Impact Factor
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    ABSTRACT: Purpose The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Materials and Methods Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay. Results The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01). Conclusion Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI.
    Yonsei Medical Journal 09/2014; 55(5):1333-40. DOI:10.3349/ymj.2014.55.5.1333 · 1.26 Impact Factor
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    ABSTRACT: ObjectiveTo evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) or brain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos.MethodsMature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1 mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance medium supplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocyst formation rate and their cell numbers were assessed.ResultsThe blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groups when compared with the control. However, the blastocyst formation rate and the total cell count was significantly higher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8±11.5 vs. 52.3%, 38.0±6.8; P<0.05, respectively).ConclusionSupplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos, but supplementation of rmGM-CSF did not.
    09/2014; 57(5):373-8. DOI:10.5468/ogs.2014.57.5.373
  • Fertility and Sterility 09/2014; 102(3):e319-e320. DOI:10.1016/j.fertnstert.2014.07.1082 · 4.30 Impact Factor
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    ABSTRACT: This prospective study investigated the relationship between anti-Müllerian hormone (AMH) level in the follicular fluid (FF) and the quality of the oocyte and embryo. A total of 65 FF samples from 54 women were included in this study. FF was collected from the largest preovulatory follicle sized≥20 mm of mean diameter from each ovary. Samples were divided into 3 groups according to the FF AMH levels: below the 33th percentile (low group, FF AMH<2.1 ng/mL, n=21), between the 33th and the 67th percentile (intermediate group, FF AMH=2.1-3.6 ng/mL, n=22), and above the 67th percentile (high group, FF AMH>3.6 ng/mL, n=22). The quality of the ensuing oocytes and embryos was evaluated by fertilization rate and embryo score. FF AMH levels correlated positively with the matched embryo score on day 3 after fertilization (r=0.331, P=0.015). The normal fertilization rate was significantly lower in the low group than in the intermediate group (61.9% vs. 95.5% vs. 77.3%, respectively, P=0.028). Our results suggest that the FF AMH level could be a predictor of the ensuing oocyte and embryo quality. Graphical Abstract
    Journal of Korean Medical Science 09/2014; 29(9):1266-70. DOI:10.3346/jkms.2014.29.9.1266 · 1.25 Impact Factor
  • Fertility and Sterility 09/2014; 102(3):e261. DOI:10.1016/j.fertnstert.2014.07.890 · 4.30 Impact Factor
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    ABSTRACT: Our aim was to investigate whether celecoxib, a cyclooxygenase 2 (COX-2) inhibitor, decreases the in vitro proliferation of leiomyoma cells if the inflammatory pathway is blocked. Menstruation is an inflammation of uterus that produces cytokines and prostanoids, but the inflammatory mechanism underlying the growth of leiomyoma remains unexplained. Using in vitro cultures of leiomyoma cells obtained from 5 patients who underwent hysterectomy, cell proliferation, inflammatory signaling, transcription factors, growth factors, and extracellular matrix were examined by (4,5-dimethylthiaxol-2-yi)-2,5-diphenyltetraxolium bromide assay, immunoblotting, and quantitative polymerase chain reaction. Prostaglandin E2 was used to induce menstruation-like condition in the cells. We found that celecoxib inhibited COX-2 through the expression of nuclear factor κB in the cells. Celcoxib also decreased the gene expression of interleukin 6, tumor necrosis factor α, collagen A, fibronectin, platelet-derived growth factor, epidermal growth factor, and transforming growth factor β. In conclusion, the present study indicated that celecoxib could inhibit leiomyoma cell proliferation through blocking the inflammatory pathway that is probably one of the mechanisms underlying its pathogenesis.
    Reproductive sciences (Thousand Oaks, Calif.) 07/2014; 21(9). DOI:10.1177/1933719114542010 · 2.18 Impact Factor
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    ABSTRACT: Objective To investigate the effects of necrostatin-1 (Nec-1) supplementation on vitrification, warming and transplantation of ovarian tissue. Study design Ovaries from 4-week-old ICR mice were vitrified using a two-step procedure; ovaries were suspended in equilibration solution for 10 min, and then mixed with vitrification solution for 5 min. Ovaries were divided at random into three groups and 0 (control), 25 or 100 μM Nec-1 was added to the vitrification solution. After warming, follicular morphology and apoptosis were assessed. For each group, a sample of vitrified, warmed ovaries was autotransplanted. The same dose of Nec-1 that was added to the vitrification solution was added to each warming solution and injected intraperitoneally. Follicular morphology and apoptosis of transplanted ovaries were assessed after 2 weeks. Results After vitrification and warming, morphological analysis revealed that the intact follicle ratio was significantly higher in the Nec-1-treated groups compared with the control group (control, 45.1%; 25 μM Nec-1, 51.7%; 100 μM Nec-1, 57.9%). The rate of apoptosis was lower in the Nec-1 treated groups compared with the control group (control, 11.2%; 25 μM Nec-1, 8.5%; 100 μM Nec-1, 7.2%). After transplantation of the vitrified, warmed ovaries, morphological analysis revealed that the intact follicle ratio was significantly higher in the Nec-1 treated groups compared with the control group (control, 43.1%; 25 μM Nec-1, 60.6%; 100 μM Nec-1, 70.7%). The rate of apoptosis was lower in the Nec-1 treated groups compared with the control group (control, 5.3%; 25 μM Nec-1, 2.5%; 100 μM Nec-1, 2.0%). Conclusions Nec-1 supplementation during vitrification, warming and transplantation has beneficial effects on the survival of ovarian tissue. These results can help to improve ovarian tissue vitrification and transplantation protocols for fertility preservation.
    European Journal of Obstetrics & Gynecology and Reproductive Biology 07/2014; 178. DOI:10.1016/j.ejogrb.2014.04.040 · 1.63 Impact Factor
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    ABSTRACT: Objective To determine whether intracytoplasmic sperm injection (ICSI) could prevent total fertilization failure (TFF) and enhance the embryo quality in patients with non-male factor infertility. Study Design Two hundred and ninety-six in vitro fertilization (IVF) cycles performed in patients with non-male factor infertility between April 2009 and March 2013 were included in this retrospective study. During the period, ICSI and conventional IVF were performed in 142 and 154 cycles, respectively. The usual indications for ICSI were in the cycles of patients with 1) known low fertilization rate, 2) repetitive implantation failure, 3) advanced maternal age, 4) presence of endometrioma, 5) low oocyte yield (number of oocytes ≤3), or 6) poor quality oocytes. The rate of TFF, normal fertilization, abnormal pronuclei (PN) formation, embryo quality, and pregnancy outcomes between the patients treated with ICSI and conventional IVF cycles were compared. Results The patients treated with ICSI (ICSI group, n = 142) presented fewer number of oocytes than patients treated with conventional IVF cycles (n = 154). The TFF rate was not different (4.2% vs. 0.6%, P = 0.059), but the ICSI group presented a significantly higher rate of normal fertilization (83.4% vs. 79.1%, P = 0.04) and lower rate of abnormal PN formation (3.9% vs. 13.3%, P < 0.01). The cleavage stage embryo quality was better in the ICSI group (grade A: 31.1% vs. 21.3%, P = 0.001; grade A + B: 65.1% vs. 47.6%, P < 0.001). Conclusion The result of this study does not support the use of ICSI to prevent TFF in patients with non-male factor infertility. However, ICSI improved the fertilization rate and the embryo quality.
    European journal of obstetrics, gynecology, and reproductive biology 07/2014; 178. DOI:10.1016/j.ejogrb.2014.03.044 · 1.63 Impact Factor

Publication Stats

868 Citations
363.42 Total Impact Points

Institutions

  • 2003–2015
    • Seoul National University Bundang Hospital
      • Department of Obstetrics and gynecology
      Sŏul, Seoul, South Korea
  • 1999–2014
    • Seoul National University
      • • Department of Obstetrics and Gynecology
      • • Medical Research Center
      • • College of Medicine
      Sŏul, Seoul, South Korea
  • 2011
    • Yonsei University Hospital
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
  • 2006–2011
    • Seoul National University Hospital
      • Department of Obstetrics and Gynecology
      Sŏul, Seoul, South Korea
  • 2008
    • McGill University
      • Department of Obstetrics and Gynecology
      Montréal, Quebec, Canada
  • 2002
    • Jeju National University
      Tse-tsiu, Jeju, South Korea