Brian K Agan

Publications (2)14.53 Total impact

• Source

  Available from: David Metzgar

  Article: Broad-spectrum respiratory tract pathogen identification using resequencing DNA microarrays.

  Baochuan Lin, Zheng Wang, Gary J Vora, Jennifer A Thornton, Joel M Schnur, Dzung C Thach, Kate M Blaney, Adam G Ligler, Anthony P Malanoski, Jose Santiago, Elizabeth A Walter, Brian K Agan, David Metzgar, Donald Seto, Luke T Daum, Russell Kruzelock, Robb K Rowley, Eric H Hanson, Clark Tibbetts, David A Stenger
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  ABSTRACT: The exponential growth of pathogen nucleic acid sequences available in public domain databases has invited their direct use in pathogen detection, identification, and surveillance strategies. DNA microarray technology has offered the potential for the direct DNA sequence analysis of a broad spectrum of pathogens of interest. However, to achieve the practical attainment of this potential, numerous technical issues, especially nucleic acid amplification, probe specificity, and interpretation strategies of sequence detection, need to be addressed. In this report, we demonstrate an approach that combines the use of a custom-designed Affymetrix resequencing Respiratory Pathogen Microarray (RPM v.1) with methods for microbial nucleic acid enrichment, random nucleic acid amplification, and automated sequence similarity searching for broad-spectrum respiratory pathogen surveillance. Successful proof-of-concept experiments, utilizing clinical samples obtained from patients presenting adenovirus or influenza virus-induced febrile respiratory illness (FRI), demonstrate the ability of this approach for correct species- and strain-level identification with unambiguous statistical interpretation at clinically relevant sensitivity levels. Our results underscore the feasibility of using this approach to expedite the early surveillance of diseases, and provide new information on the incidence of multiple pathogens.
  Genome Research 05/2006; 16(4):527-35. · 13.61 Impact Factor
• Article: Potential use of microarray technology for rapid identification of central nervous system pathogens.

  Eric H Hanson, Debra M Niemeyer, Les Folio, Brian K Agan, Robb K Rowley
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  ABSTRACT: Outbreaks of central nervous system (CNS) diseases result in significant productivity and financial losses, threatening peace and wartime readiness capabilities. To meet this threat, rapid clinical diagnostic tools for detecting and identifying CNS pathogens are needed. Current tools and techniques cannot efficiently deal with CNS pathogen diversity; they cannot provide real-time identification of pathogen serogroups and strains, and they require days, sometimes weeks, for examination of tissue culture. Rapid and precise CNS pathogen diagnostics are needed to provide the opportunity for tailored therapeutic regimens and focused preventive efforts to decrease morbidity and mortality. Such diagnostics are available through genetic and genomic technologies, which have the potential for reducing the time required in serogroup or strain identification from 500+ hours for some viral cultures to less than 3 hours for all pathogens. In the near future, microarray diagnostics and future derivations of these technologies will change the paradigm used for outbreak investigations and will improve health care for all.
  Military medicine 09/2004; 169(8):594-9. · 0.92 Impact Factor