Chinese medical journal 10/2005; 118(17):1487-9. · 0.86 Impact Factor
ABSTRACT: To detect the level and dynamic change of severe acute respiratory syndrome (SARS)-coronavirus-specific IgG antibody in conavalescent SARS patients, and to provide information for prevention and vaccine development.
IgG antibody against coronavirus was detected by ELISA in the blood of 311 convalescent SARS patients for every 2 - 4 weeks. Stata 7.0 statistics software was used to analyse the results.
IgG antibody was detected positive on each testing of all the convalescent patients and its peak appeared 35 days after recovery. IgG antibody level showed a 35.8% decrease within one year.
Data showed that all the SARS convalescent patients had generated high level of specific IgG antibody against coronavirus in the early stage of recovery, but the antibody level declined along with the progress of convalescence, suggesting that the detection of the IgG antibody should go on until it disappeared.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 04/2005; 26(3):194-6.
ABSTRACT: To investigate the dynamic changes of the antibody specific to severe acute respiratory syndrome (SARS) coronavirus in convalescents who have suffered from SARS.
Samples of peripheral blood were collected twice during the first 2 weeks after discharge and then once every 2 - 4 weeks from 310 convalescents of SARS, 131 males and 179 females, aged 18 - 74, discharged from hospitals in Beijing April 3 to June 20 2003 with the average discharge date of June 10, to detect the level of immunoglobulin G (IgG) by ELISA. A curve of level of IgG was drawn to describe the change by the specific month in the year.
Most of the 310 convalescents underwent successive or non-successive testing for 2 - 4 months and 15 were tested for 5 months, 15 for 6 months, and 2 for 7 months. IgG was detected in each sample with the mean of A value of 0.97 +/- 0.37 (0.197 - 1.849). The mean IgG level peaked in July (1.203), about 35 days after discharge, and then gradually declined to 0.857 in December, a decline by 27.3%.
All SARS patients generate specific antibody against the coronavirus. However, the antibody level gradually decline with the lapse of time during the convalescence. Long-term surveillance of the change of antibody is necessary.
Zhonghua yi xue za zhi 11/2004; 84(20):1690-2.
ABSTRACT: To investigate clinical characteristics and long-term effects of SARS.
Clinical characteristics of 197 SARS patients in Xiao Tang-shan hospital were analyzed retrospectively, and prognosis of them were analyzed prospectively.
Among the 197 patients, 153 patients (77.7%) have positive results of serum SARS coronavirus-specific antibodies test, and 44 patients (22.3%) have negative results. The average age of SARS and non-SARS patients were (40 +/- 12) and (31 +/- 12) years, male/female ratio were 1.0:1.6 and 2.1:1.0, average body temperature were (38.5 degrees C +/- 0.3 degrees C) and (38.1 degrees C +/- 0.4 degrees C), median of fever length were 7.0 d (0.4 approximately 50 d) and 2.3 d (0.3 approximately 37 d) respectively. The occurrence of dyspnea, malaise and gastrointestinal symptom were more often in SARS patient than in non-SARS patients. Some patients have residual symptoms (such as cough, fatigue, dyspnea, abnormality of liver function, hyperglycemia and hyperglyceridemia), and only few patients have lung fibrosis.
Some patients with other respiratory diseases were misdiagnosed as SARS. There were several obvious differences of clinical characteristics between SARS patient and non-SARS patients. Prognosis of most patients were preciously well, and few still had abnormality of lung function. Residual symptoms of SARS and side effects of drugs used to treat SARS should be discriminated.
Zhonghua yi xue za zhi 05/2004; 84(8):642-5.
ABSTRACT: To analyze the clinical characteristics and prognostic changes of rehabilitating severe acute respiratory syndrome (SARS) patients through regular lung function tests and lung imaging studies after discharge and to retrospectively analyze the treatment data of these patients.
258 discharged SARS patients received regular SARS-Co virus IgG test, lung function test and chest X-ray and/or high resolution computerized tomography (HRCT) examination at General Hospital of PLA two months after discharge, and the treatment data of these patients were retrospectively analyzed.
80.6% patients (208 of 258 patients) were positive for SARS-Co virus IgG. 21.3% patients (55 of 258 patients) showed lung diffusion abnormity (D(LCO) < 80%pred). Compared to 155 SARS-Co virus IgG positive patients without lung diffusion abnormity and 50 SARS-Co virus IgG negative patients, the 53 SARS-Co virus IgG positive patients with lung diffusion abnormity had longer fever course, higher dosages of glucocorticoid therapy, higher percentage of oxygen therapy and non-invasive ventilation. 51 of the 53 patients with lung diffusion abnormity received lung function test after one month, and the results of D(LCO) improved in 80.4% patients (41 of 51 patients). 40 of 51 patients with lung diffusion abnormity showed lung fibrosis, and the fibrosis decreased in 55% patients (22 of 40 patients) after one month.
This finding suggests that lung fibrosis caused by SARS mostly occurs in severe patients, and it can resolve spontaneously. D(LCO) may be more sensitive than HRCT in evaluating the fibrotic changes.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 04/2004; 27(3):147-50.
ABSTRACT: To study the serum anti-coronavirus antibody titer in medical personnel who had closely contacted with severe acute respiratory syndrome (SARS) patients.
The serum anti-coronavirus IgG antibody titer in medical personnel who had closely contacted with SARS patients, healthy individuals, patients with community acquired pneumonia and patients recovered from SARS was detected by using an enzyme-linked immunosorbent assay (ELISA) method. The antibody titer was expressed as the value of absorbency (A) with common logarithm conversion.
The serum anti-coronavirus IgG antibody titer in patients recovered from SARS was 0.07 +/- 0.13, which was significantly higher as compared with those in other groups. The antibody titer in medical personnel was -1.18 +/- 0.20, which was also significantly higher as compared with those in community acquired pneumonia patients and healthy persons. In the healthy persons, the antibody titer of serum samples obtained from Beijing in May, 2003 was -1.61 +/- 0.13, which was significantly higher than that of samples obtained from Beijing in 2001 when SARS was not found -1.76 +/- 0.25 and that of samples from Shandong province where SARS was not found in May, 2003 -1.95 +/- 0.44. There was no significant difference in the antibody titer between patients of bacterial pneumonia and patients of atypical pneumonia, which was -1.99 +/- 0.31 and -2.05 +/- 0.23 respectively.
Close contact with SARS patients can cause the serum anti-coronavirus antibody titer to increase significantly in medical personnel, a phenomenon deserves further study.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 11/2003; 26(10):583-5.
ABSTRACT: It is clear from works already reported that depleted uranium (DU) affect human health. However, the late effect, especially the carcinogenesis, was not clearly understood. This study was designed to investigate the malignant transformation of human bronchial epithelial cell induced by insoluble DU and lung cancer related gene expression pattern, through imitating the condition that human absorbs depleted uranium aerosol.
Adenovirus-12/SV40 virus immortalized human bronchial epithelial cells (BEAS-2B) were reacted with insoluble DU oxide (dUO2); the characteristics of malignant transformation of cells were identified through observing the multiplication time of different generation cells, serum resistance, colony formation rate of semi-solid agar, and tumorigenesis in nude mice. Gene expression pattern of transferred BEAS-2B cell induced by DU was determined using 213 lung cancer related gene arrays.
The multiplication time of BEAS-2B cell treated with DU was obviously decreased and the serum reistance was significantly increased in 5th generation; the anchorage independent growth (semi-solid agar colony formation) was appeared in 10th generation cell. The 15th generation cell formed tumor in nude mice. DMSO showed overt protection effect on malignant transformation of BEAS-2B cell. The analyzing results of 213 lung cancer related gene arrays showed that the expression level changed in more than 70 genes of transferred cells, including the overt decrease of level of gene expression in more than 10 genes.
DU has carcinogenesis in vitro.
Ai zheng = Aizheng = Chinese journal of cancer 09/2002; 21(9):944-8.
ABSTRACT: Objective: To detect the expression difference of 60 lung cancer associated genes in human bronchial epithelial malignant
transformation cell model (BEP2D) induced by alpha-particles. Methods: 60 lung cancer associated genes were collected and
micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cells and
passage 20 (R15H-20), passage 35 (R15H-35) cells derived from BEP2D following 1.5 Gy alpha-particles was extracted and labeled
by fluorescent dye. The labeled probe was then hybridized with the cDNA. Results: 40, 47, 20 genes were detected in BEP2D,
R15H-20 and R15H-35 respectively. The expression level of tumor suppressor genes decreased greatly in the transformed R15H-35.
Most oncogenes decreased slightly in R15H-20. Most growth factors expressed only in R15H-20. Conclusion: In human bronchial
epithelial malignant transformed cell model generated by alpha-particles, the loss-function of tumor suppressor genes at initiation
stage was dominant, some related oncogenes and growth factors promoted the malignant transformation.
Chinese Journal of Cancer Research 02/2002; 14(1):18-23. · 0.18 Impact Factor
ABSTRACT: Escape from transforming growth factor-beta(TGF-beta)-induced inhibition of growth and proliferation may contribute to tumorigenesis. Smad7 is inhibitory Smads of TGF-beta s signal transduction pathway and prevents TGF-beta signaling. The disorder of Smad7 may lead to the perturbation of TGF-beta signal pathway. In this study, The authors analyzed the expression of Smad7 mRNA and the regulation of Smad7 gene by TGF-beta 1 in the process of malignant transformation of BEP2D cells to investigate the mechanism of cells malignant transformation.
Cells were cultured and stimulated with TGF-beta 1 followed by RNA extraction. Purified total RNA from TGF-beta 1 treated cells and untreated controls and performed an expression analysis with a human Smad7-specific probe applying Northern blot. As a loading control for the Northern experiment, the membrane was hybridized with a human glyceraldehyde-3-phosphate dehydrogenase(GAPDH) probe. Proteins were extracted from BEP2D and BERP35T-2 cells, then perform Western blot to examine the expression level of TGF-beta 1.
Before stimulation with TGF-beta 1, the expression level of Smad7 in the BERP35T-2 cells were higher than that in the BEP2D cells. When stimulated with TGF-beta 1, Smad7 expression levels was upregulated evidently in BEP2D cells, but not significant in BERP35T-2 cells. The expression level of endogenetic TGF-beta 1, BERP35T-2 cells was a little higher than BEP2D cells.
Over expression of Smad7 mRNA and down-regulation of the cells' responsiveness to TGF-beta 1 in human lung cancer cell line which induced by alpha-particles should be one of the mechanism of radiation induced lung cancer.
Ai zheng = Aizheng = Chinese journal of cancer 02/2002; 21(2):117-21.
ABSTRACT: Objective: Smad7 was identified as a TGF-β-inducible antagonist of TGF-β signaling and might participate in a negative feedback
loop to control TGF-β signaling. In this study, the responsiveness of Smad7 to TGF-β1 was examined in the BEP2D and BERP35T-2
cells to investigate the possible mechanism of Smad7 in the tumorigenesis. Methods: Northern and western blot were performed
to exam the Smad7 and TGF-β1 expression abundance in BEP2D and BERP35T-2 at both transcription and translation level. Results:
The expression level of Smad7 mRNA in BERP35T-2 cells was higher than that in BEP2D cells. When stimulated with TGF-β1, Smad7
expression was up-regulated evidently in BEP2D cells, but not significantly in BERP35T-2 cells. The abundance of TGF-β1 in
the cytoplasm of BERP35T-2 was not significantly higher than in BEP2D (P>0.05); whereas the abundance of TGF-β1 in BERP35T-2 cell culture medium was significantly higher than in BEP2D cell culture
medium (P<0.05). Conclusion: Over expression of Smad7 mRNA and down-regulation of the cells’ responsiveness to TGF-β1 in human lung
cancer cell line might be involved in lung carcinogenisis.
Chinese Journal of Cancer Research 01/2002; 14(3):170-174. · 0.18 Impact Factor