Bao-Liang Li

Publications (2)0.82 Total impact

• Article: [Mechanism of multiple organ injury subsequent to acute renal failure with respect to membrane pump activity in rabbits].

  Rui Han, Bao-liang Li, Chun-yu Niu, Yu-ping Zhang, Ya-li Hou, Zi-gang Zhao
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  ABSTRACT: To observe the changes in membrane pump activity of kidney, myocardium and pancreas in rabbits with acute renal failure (ARF) in rabbits, and inquire into the mechanism of multiple organ injury subsequent to ARF. Forty-two rabbits were randomly divided into control group, HgCl(2) group and glycerine group, and the latter two groups were subdivided into 12, 24, 48-hour subgroups, with 6 rabbits in each group. The ARF model was reproduced by hypodermic injection 1% HgCl(2) (1.3 ml/kg) in HgCl(2) group, or intramuscularly injection 50% glycerine (10 ml/kg) in glycerine group, respectively. At different time points, the kidney, myocardium and pancreas were harvested, and homogenates of them were prepared. The ATPase activities of different organ homogenates were determined. It showed that the activities of Na(+)-K(+)-ATPase, Ca(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase of renal homogenate in two model groups were reduced gradually with worsening of renal function, and they became lowest at 48 hours [(0.84±0.16), (0.52±0.17), (0.45±0.09) μmol×mg(-1)×h(-1) in HgCl(2) group; (0.85±0.22), (0.49±0.21), (0.54±0.17) μmol×mg(-1) ×h(-1)in glycerine group]. The respective activities of Na (+)-K (+)-ATPase, Ca (2+)-ATPase, Mg(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase of myocardium and pancreas homogenates in two model groups were reduced gradually following depression of renal function, and they became lowest at 48 hours [(0.56±0.11), (0.51±0.19), (0.55±0.19), (0.37±0.19) μmol×mg(-1)×h(-1) in HgCl(2) group and (0.52±0.19), (0.62±0.10), (0.61±0.16), (0.54±0.10) μmol×mg(-1) ×h(-1) in myocardium homogenate of glycerine group; (0.81±0.12), (0.71±0.15), (0.73±0.18), (0.62±0.16) μmol×mg(-1) ×h(-1) in HgCl(2) group and (0.72±0.13), (0.57±0.18), (0.66±0.14), (0.59±0.23) μmol×mg(-1) ×h(-1) in pancreas homogenate of glycerine group], there was statistical difference compared with control group (P<0.05 or P<0.01). The mechanism of myocardial and pancreatic injury subsequent to ARF might be related to reduction of the activity of cell membrane pump.
  Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 12/2010; 22(12):733-5.
• Article: The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities.

  Zi-Gang Zhao, Chun-Yu Niu, Yu-Ping Zhang, Rui Han, Ya-Li Hou, Bao-liang Li, Shu-ting Du
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  ABSTRACT: There is increasing evidence indicating that the distant organ injury is a major contributor of high mortality in patients subjected to acute renal failure (ARF). However, sources and mechanisms that ARF causes distant organ injury remain to be determined. The aim of this study is to explore the mechanism from polymorphonuclear neutrophil (PMN) sequestration and membrane pump suppression. To achieve this, we examined myeloperoxidase (MPO), a marker of PMN accumulation in tissues, and membrane pump activities of heart, pancreas, and kidney in two ARF rabbit models. Rabbits are randomly assigned to control, HgCl(2)-treated, and glycerin-treated groups. ARF animal models are established by hypodermic injection of 1% HgCl(2) with 1.3 mL/kg bodyweight (bw) in HgCl(2)-treated group or intramuscular injection of 50% glycerin with 10 mL/kg bw in glycerin-treated group, respectively, and all animals in each group are further divided into 12 h, 24 h, and 48 h subgroups with each consisting of six rabbits. Six healthy rabbits serve as control group. Results have shown that MPO activities of kidney, myocardium, and pancreas in two model groups were significantly increased than control group at diverse time points. Membrane pump activities of kidney in two model groups are significantly lower than the control group at multiple time points. Moreover, Na(+)-K(+)-, Ca(2+)-, Mg(2+)-, and Ca(2+)-Mg(2+)-ATPase activities of myocardium and pancreas in two model groups are gradually declined with the development of ARF. These findings suggest that PMN sequestration and membrane pump suppression plays an important role in the pathogenesis of ARF and also a major mechanism of myocardium and pancreas injury during the process of ARF.
  Renal Failure 01/2010; 32(10):1216-22. · 0.82 Impact Factor