Andrew D Luster

Massachusetts General Hospital, Boston, Massachusetts, United States

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Publications (321)3150.12 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Background: Microvesicles (MVs) (100-1000 nm diameter) are subcellular particles that are enriched in nucleic acid, including microRNA (miR), which may be transferred from cell to cell as a novel form of intercellular communication. In this study, we wished to characterize the microRNA content of microvesicles purified from the synovial fluid of patients with osteoarthritis or rheumatoid arthritis. Furthermore, we wished to determine whether predominant miR species identified in RA SF MVs may be transferred to recipient fibroblast-like synoviocytes (FLS) in vitro. Methods: Discarded synovial fluid (SF) from patients with osteoarthritis (OA) (n=5) or rheumatoid arthritis (RA) (n=5) obtained during routine clinical care was transferred into tubes containing acid-citrate-dextrose and stored at 4°C until further processing. SF was treated with thromboplastin D, centrifuged at 300 g x 10 minutes and 3000 g x 15 minutes to remove cells and cellular debris, respectively, and filtered through a 0.8 m filter. Next, SF was placed into ultracentrifuge tubes diluted with sterile PBS in a 1:8 ratio and ultracentrifuged at 100,000 g x 90 min using a Beckman 70.1Ti rotor. Purified MV pellets were resuspended in sterile PBS at one-fifth the original volume and quantified using a Nanosight LM10 instrument. MicroRNA from purified MV pellets was purified using a miRNeasy Serum/Plasma Kit (Qiagen), reverse transcribed, and subject to miRNA analysis using miScript miRNA PCR Serum/Plasma Arrays. PCR array data were analyzed using the Qiagen GeneGlobe Data Analysis Center online software tool, normalizing to global CT mean of expressed miRNAs, using a cutoff of CT=37. Purified RA SF MVs were incubated with OA FLS for 16 hours, and pri-miR, pre-miR, and mature miR expression by FLS was determined by qPCR. Results: Purified RA SF MVs purified in this manner were significantly greater than OA SF MVs (OA vs. RA 24.95 vs. 53.55 million/l, p=0.0001). A total of 39 miRs showed greater than 2-fold statistically significant differences in expression between groups (p<0.05). Of these miRs, hsa-mir-223-3p expression was significantly upregulated in the RA SF MV group (102.4 fold regulation, p=0.005) when compared with the OA SF MV group. Addition of purified RA SF MVs to cultured human FLS induced expression of mir-223, but not pri-miR-223 or pre-mir-223, indicating that mir-223 in FLS was of exogenous (i.e. microvesicle) origin. Conclusion: Microvesicle numbers in RA synovial fluid are greater than in OA synovial fluid, consistent with other previously published reports. We found significantly increased expression of mir-223-3p in RA SF MV, likely reflecting myeloid cell activation in the inflamed joint, and demonstrated that mir-223 may be transferred to recipient FLS in vitro.
    2015 American College of Rheumatology/ ARHP Annual Meeting, San Francisco, CA; 11/2015

  • Chest 10/2015; 148(4_MeetingAbstracts):561A. DOI:10.1378/chest.2260159 · 7.48 Impact Factor
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    ABSTRACT: Anti-CD3 therapy of type 1 diabetes results in a temporary halt of the pathogenesis but does not constitute a permanent cure. One problem is the re-infiltration of the islets of Langerhans with regenerated, autoaggressive lymphocytes. We aimed at blocking such a re-entry by neutralizing the key chemokine CXCL10. Combination therapy of diabetic RIP-LCMV and NOD mice with anti-CD3 and anti-CXCL10 antibodies caused a substantial remission of diabetes and was superior to monotherapies with anti-CD3 or anti-CXCL10 alone. The combination therapy prevented islet-specific T cells from re-entry of the islets of Langerhans and thereby blocked the autodestructive process. In addition, the local immune balance in the pancreas was shifted towards a regulatory phenotype. A sequential temporal inactivation of T cells and blockade of T cell migration might constitute a novel therapy for patients with type 1 diabetes. © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
    Diabetes 08/2015; DOI:10.2337/db15-0479 · 8.10 Impact Factor
  • Nancy D Kim · Andrew D Luster ·
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    ABSTRACT: Neutrophils are first responders of the immune system, rapidly migrating into affected tissues in response to injury or infection. To effectively call in this first line of defense, strategically placed cells within the vasculature and tissue respond to noxious stimuli by sending out coordinated signals that recruit neutrophils. Regulation of organ-specific neutrophil entry occurs at two levels. First, the vasculature supplying the organ provides cues for neutrophil egress out of the bloodstream in a manner dependent upon its unique cellular composition and architectural features. Second, resident immune cells and stromal cells within the organ send coordinated signals that guide neutrophils to their final destination. Here, we review recent findings that highlight the importance of these tissue-specific responses in the regulation of neutrophil recruitment and the initiation and resolution of inflammation. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Trends in Immunology 08/2015; 36(9). DOI:10.1016/ · 10.40 Impact Factor
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    ABSTRACT: Initial events after exposure determine HIV-1 disease progression, underscoring a critical need to understand host mechanisms that interfere with initial viral replication. Although associated with chronic HIV-1 control, it is not known whether interleukin-21 (IL-21) contributes to early HIV-1 immunity. Here we take advantage of tractable primary human lymphoid organ aggregate cultures to show that IL-21 directly suppresses HIV-1 replication, and identify microRNA-29 (miR-29) as an antiviral factor induced by IL-21 in CD4 T cells. IL-21 promotes transcription of all miR-29 species through STAT3, whose binding to putative regulatory regions within the MIR29 gene is enriched by IL-21 signalling. Notably, exogenous IL-21 limits early HIV-1 infection in humanized mice, and lower viremia in vivo is associated with higher miR-29 expression. Together, these findings reveal a novel antiviral IL-21-miR-29 axis that promotes CD4 T-cell-intrinsic resistance to HIV-1 infection, and suggest a role for IL-21 in initial HIV-1 control in vivo.
    Nature Communications 06/2015; 6:7562. DOI:10.1038/ncomms8562 · 11.47 Impact Factor
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    ABSTRACT: T-cell trafficking at vascular sites has emerged as a key step in antitumour immunity. Chemokines are credited with guiding the multistep recruitment of CD8(+) T cells across tumour vessels. However, the multiplicity of chemokines within tumours has obscured the contributions of individual chemokine receptor/chemokine pairs to this process. Moreover, recent studies have challenged whether T cells require chemokine receptor signalling at effector sites. Here we investigate the hierarchy of chemokine receptor requirements during T-cell trafficking to murine and human melanoma. These studies reveal a non-redundant role for Gαi-coupled CXCR3 in stabilizing intravascular adhesion and extravasation of adoptively transferred CD8(+) effectors that is indispensable for therapeutic efficacy. In contrast, functional CCR2 and CCR5 on CD8(+) effectors fail to support trafficking despite the presence of intratumoral cognate chemokines. Taken together, these studies identify CXCR3-mediated trafficking at the tumour vascular interface as a critical checkpoint to effective T-cell-based cancer immunotherapy.
    Nature Communications 06/2015; 6:7458. DOI:10.1038/ncomms8458 · 11.47 Impact Factor
  • Jeffrey Lian · Andrew D Luster ·
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    ABSTRACT: The generation of adaptive immune responses occurs in the lymph node (LN) and requires that lymphocytes locate and interact with cognate antigen-bearing dendritic cells. This process requires the coordinated movement of both innate and adaptive immune cells, and is orchestrated by the chemokine family of chemotactic cytokines. Upon initiation of inflammation, the LN undergoes dramatic changes that include the marked induction of specific chemokines in distinct regions of the reactive LN. These chemokine rich domains establish LN niches that facilitate the differentiation of CD4+ T cells into effector cell subsets and the rapid activation of memory CD8+ T cells. This review will focus on recent advances highlighting the importance of LN chemokines for shaping adaptive immune responses by controlling immune cell migration, positioning, and interactions in the reactive LN. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Current opinion in cell biology 06/2015; 36:1-6. DOI:10.1016/ · 8.47 Impact Factor
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    ABSTRACT: Chronic exposure to crystalline silica (CS) causes silicosis, an irreversible lung inflammatory disease that may eventually lead to lung cancer. In this study, we demonstrate that in K-ras LA1 mice, CS exposure markedly enhances the lung tumour burden and genetic deletion of leukotriene B4 receptor-1 (BLT1 À / À) attenuates this increase. Pulmonary neutrophilic inflammation induced by CS is significantly reduced in BLT1 À / À K-ras LA1 mice. CS exposure induces LTB 4 production by mast cells and macrophages independent of inflammasome activation. In an air-pouch model, CS-induced neutrophil recruitment is dependent on LTB 4 production by mast cells and BLT1 expression on neutrophils. In an implantable lung tumour model, CS exposure results in rapid tumour growth and decreased survival that is attenuated in the absence of BLT1. These results suggest that the LTB 4 /BLT1 axis sets the pace of CS-induced sterile inflammation that promotes lung cancer progression. This knowledge may facilitate development of immunotherapeutic strategies to fight silicosis and lung cancer.
    Nature Communications 04/2015; 6. DOI:10.1038/ncomms8064 · 11.47 Impact Factor
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    ABSTRACT: The molecules and pathways that fine-tune innate inflammatory responses mediated by Toll-like receptor 7 (TLR7) remain to be fully elucidated. Using an unbiased genome-scale screen with short hairpin RNA (shRNA), we identified the receptor TREML4 as an essential positive regulator of TLR7 signaling. Macrophages from Treml4(-/-) mice were hyporesponsive to TLR7 agonists and failed to produce type I interferons due to impaired phosphorylation of the transcription factor STAT1 by the mitogen-activated protein kinase p38 and decreased recruitment of the adaptor MyD88 to TLR7. TREML4 deficiency reduced the production of inflammatory cytokines and autoantibodies in MRL/lpr mice, which are prone to systemic lupus erythematosus (SLE), and inhibited the antiviral immune response to influenza virus. Our data identify TREML4 as a positive regulator of TLR7 signaling and provide insight into the molecular mechanisms that control antiviral immunity and the development of autoimmunity.
    Nature Immunology 04/2015; 16(5). DOI:10.1038/ni.3143 · 20.00 Impact Factor
  • Maud Deruaz · Andrew D Luster ·
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    ABSTRACT: The genital tract mucosa is the site where sexually transmitted infections gain entry to the host. The immune response at this site is thus critical to provide innate protection against pathogens that are seen for the very first time as well as provide long-term pathogen-specific immunity, which would be required for an effective vaccine against sexually transmitted infection. A finely regulated immune response is therefore required to provide an effective barrier against pathogens without compromising the capacity of the genital tract to allow for successful conception and fetal development. We review recent developments in our understanding of the immune response in the female genital tract to infectious pathogens, using herpes simplex virus-2, human immunodeficiency virus-1 and Chlamydia trachomatis as examples, with a particular focus on the role of chemokines in orchestrating immune cell migration necessary to achieve effective innate and adaptive immune responses in the female genital tract.Immunology and Cell Biology advance online publication, 17 March 2015; doi:10.1038/icb.2015.20.
    Immunology and Cell Biology 03/2015; 93(4). DOI:10.1038/icb.2015.20 · 4.15 Impact Factor
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    Caroline L Sokol · Andrew D Luster ·
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    ABSTRACT: Chemokines are chemotactic cytokines that control the migration and positioning of immune cells in tissues and are critical for the function of the innate immune system. Chemokines control the release of innate immune cells from the bone marrow during homeostasis as well as in response to infection and inflammation. They also recruit innate immune effectors out of the circulation and into the tissue where, in collaboration with other chemoattractants, they guide these cells to the very sites of tissue injury. Chemokine function is also critical for the positioning of innate immune sentinels in peripheral tissue and then, following innate immune activation, guiding these activated cells to the draining lymph node to initiate and imprint an adaptive immune response. In this review, we will highlight recent advances in understanding how chemokine function regulates the movement and positioning of innate immune cells at homeostasis and in response to acute inflammation, and then we will review how chemokine-mediated innate immune cell trafficking plays an essential role in linking the innate and adaptive immune responses. Copyright © 2015 Cold Spring Harbor Laboratory Press; all rights reserved.
    Cold Spring Harbor perspectives in biology 01/2015; 7(5). DOI:10.1101/cshperspect.a016303 · 8.68 Impact Factor
  • Zamaneh Mikhak · William W. Agace · Andrew D. Luster ·
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    ABSTRACT: Lymphocytes are the key cells of the adaptive immune system that provide antigen-specific responses tailored to the context of antigen exposure. Through cytokine release and antibody production, lymphocytes orchestrate and amplify the recruitment and function of other immune cells and contribute to host defense against invading pathogens and the pathogenesis of many inflammatory diseases. Lymphocyte function is critically dependent on their ability to traffic into the correct anatomic locations at the appropriate times. This process is highly regulated and requires that lymphocytes interact with various homing molecules and respond to tightly regulated navigational cues.
  • Melvyn T Chow · Andrew D Luster ·
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    ABSTRACT: Chemokines are chemotactic cytokines that control the migration of cells between tissues and the positioning and interactions of cells within tissue. The chemokine superfamily consists of approximately 50 endogenous chemokine ligands and 20 G protein-coupled seven-transmembrane spanning signaling receptors. Chemokines mediate the host response to cancer by directing the trafficking of leukocytes into the tumor microenvironment. This migratory response is complex and consists of diverse leukocyte subsets with both antitumor and protumor activities. Although chemokines were initially appreciated as important mediators of immune cell migration, we now know that they also play important roles in the biology of nonimmune cells important for tumor growth and progression. Chemokines can directly modulate the growth of tumors by inducing the proliferation of cancer cells and preventing their apoptosis. They also direct tumor cell movement required for metastasis. Chemokines can also indirectly modulate tumor growth through their effects on tumor stromal cells and by inducing the release of growth and angiogenic factors from cells in the tumor microenvironment. In this Masters of Immunology primer, we focus on recent advances in understanding the complex nature of the chemokine system in tumor biology with a focus on how the chemokine system could be used to augment cancer immunotherapeutic strategies to elicit a more robust and long-lasting host antitumor immune response. Cancer Immunol Res; 2(12); 1125-31. ©2014 AACR. ©2014 American Association for Cancer Research.
    12/2014; 2(12):1125-1131. DOI:10.1158/2326-6066.CIR-14-0160
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    ABSTRACT: Dendritic cells (DCs) are well established as potent antigen-presenting cells critical to adaptive immunity. In vaccination approaches, appropriately stimulating lymph node-resident DCs (LNDCs) is highly relevant to effective immunization. Although LNDCs have been implicated in immune response, their ability to directly drive effective immunity to lymph-borne antigen remains unclear. Using an inactive influenza vaccine model and whole node imaging approaches, we observed surprising responsiveness of LNDC populations to vaccine arrival resulting in a transnodal repositioning into specific antigen collection sites within minutes after immunization. Once there, LNDCs acquired viral antigen and initiated activation of viral specific CD4(+) T cells, resulting in germinal center formation and B cell memory in the absence of skin migratory DCs. Together, these results demonstrate an unexpected stimulatory role for LNDCs where they are capable of rapidly locating viral antigen, driving early activation of T cell populations, and independently establishing functional immune response.
    Journal of Experimental Medicine 07/2014; 211(8). DOI:10.1084/jem.20132327 · 12.52 Impact Factor
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    ABSTRACT: Obesity associates with increased numbers of inflammatory cells in adipose tissue (AT), including T cells, but the mechanism of T-cell recruitment remains unknown. This study tested the hypothesis that the chemokine receptor CXCR3 participates in T-cell accumulation in AT of obese mice and thus in the regulation of local inflammation and systemic metabolism. Obese wild-type mice exhibited higher mRNA expression of CXCR3 in periepididymal AT-derived stromal vascular cells compared with lean mice. We evaluated the function of CXCR3 in AT inflammation in vivo using CXCR3-deficient and wild-type control mice that consumed a high-fat diet. Periepididymal AT from obese CXCR3-deficient mice contained fewer T cells than obese controls after 8 and 16 weeks on high-fat diet, as assessed by flow cytometry. Obese CXCR3-deficient mice had greater glucose tolerance than obese controls after 8 weeks, but not after 16 weeks. CXCR3-deficient mice fed high-fat diet had reduced mRNA expression of proinflammatory mediators, such as monocyte chemoattractant protein-1 and regulated on activation, normal T cell expressed and secreted, and anti-inflammatory genes, such as Foxp3, IL-10, and arginase-1 in periepididymal AT, compared with obese controls. These results demonstrate that CXCR3 contributes to T-cell accumulation in periepididymal AT of obese mice. Our results also suggest that CXCR3 regulates the accumulation of distinct subsets of T cells and that the ratio between these functional subsets across time likely modulates local inflammation and systemic metabolism.
    Arteriosclerosis Thrombosis and Vascular Biology 05/2014; 34(7). DOI:10.1161/ATVBAHA.113.303133 · 6.00 Impact Factor
  • Jason W Griffith · Caroline L Sokol · Andrew D Luster ·
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    ABSTRACT: Chemokines are chemotactic cytokines that control the migratory patterns and positioning of all immune cells. Although chemokines were initially appreciated as important mediators of acute inflammation, we now know that this complex system of approximately 50 endogenous chemokine ligands and 20 G protein-coupled seven-transmembrane signaling receptors is also critical for the generation of primary and secondary adaptive cellular and humoral immune responses. Recent studies demonstrate important roles for the chemokine system in the priming of naive T cells, in cell fate decisions such as effector and memory cell differentiation, and in regulatory T cell function. In this review, we focus on recent advances in understanding how the chemokine system orchestrates immune cell migration and positioning at the organismic level in homeostasis, in acute inflammation, and during the generation and regulation of adoptive primary and secondary immune responses in the lymphoid system and peripheral nonlymphoid tissue.
    Annual Review of Immunology 03/2014; 32(1):659-702. DOI:10.1146/annurev-immunol-032713-120145 · 39.33 Impact Factor
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    ABSTRACT: CCL1 is a naturally glycosylated chemokine protein that is secreted by activated T-cells and acts as a chemoattractant for monocytes.1 Originally, CCL1 was identified as a 73 amino acid protein having one N-glycosylation site,1 and a variant 74 residue non-glycosylated form, Ser-CCL1, has also been described.2 There are no systematic studies of the effect of glycosylation on the biological activities of either CCL1 or Ser-CCL1. Here we report the total chemical syntheses of both N-glycosylated and non-glycosylated forms of (Ser-)CCL1, by convergent native chemical ligation. We used an N-glycan isolated from hen egg yolk together with the Nbz linker for Fmoc chemistry solid phase synthesis of the glycopeptide-αthioester building block.3 Chemotaxis assays of these glycoproteins and the corresponding non-glycosylated proteins were carried out. The results were correlated with the chemical structures of the (glyco)protein molecules. To the best of our knowledge, these are the first investigations of the effect of glycosylation on the chemotactic activity of the chemokine (Ser-)CCL1 using homogeneous N-glycosylated protein molecules of defined covalent structure.
    Angewandte Chemie International Edition 03/2014; 53(20). DOI:10.1002/anie.201310574 · 11.26 Impact Factor
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    ABSTRACT: Vitiligo is an autoimmune disease of the skin that results in disfiguring white spots. There are no U.S. Food and Drug Administration-approved treatments for vitiligo, and most off-label treatments yield unsatisfactory results. Vitiligo patients have increased numbers of autoreactive, melanocyte-specific CD8(+) T cells in the skin and blood, which are directly responsible for melanocyte destruction. We report that gene expression in lesional skin from vitiligo patients revealed an interferon-γ (IFN-γ)-specific signature, including the chemokine CXCL10. CXCL10 was elevated in both vitiligo patient skin and serum, and CXCR3, its receptor, was expressed on pathogenic T cells. To address the function of CXCL10 in vitiligo, we used a mouse model of disease that also exhibited an IFN-γ-specific gene signature, expression of CXCL10 in the skin, and up-regulation of CXCR3 on antigen-specific T cells. Mice that received Cxcr3(-/-) T cells developed minimal depigmentation, as did mice lacking Cxcl10 or treated with CXCL10-neutralizing antibody. CXCL9 promoted autoreactive T cell global recruitment to the skin but not effector function, whereas CXCL10 was required for effector function and localization within the skin. Surprisingly, CXCL10 neutralization in mice with established, widespread depigmentation induces reversal of disease, evidenced by repigmentation. These data identify a critical role for CXCL10 in both the progression and maintenance of vitiligo and thereby support inhibiting CXCL10 as a targeted treatment strategy.
    Science translational medicine 02/2014; 6(223):223ra23. DOI:10.1126/scitranslmed.3007811 · 15.84 Impact Factor
  • Morris Ling · Sabina Islam · John Leung · Wayne G. Shreffler · Andrew D. Luster ·

    Journal of Allergy and Clinical Immunology 02/2014; 133(2):AB170. DOI:10.1016/j.jaci.2013.12.612 · 11.48 Impact Factor
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    ABSTRACT: The transcriptional repressor B lymphocyte-induced maturation protein 1 (BLIMP1) is a master regulator of B and T cell differentiation. To examine the role of BLIMP1 in innate immunity, we used a conditional knockout (CKO) of Blimp1 in myeloid cells and found that Blimp1 CKO mice were protected from lethal infection induced by Listeria monocytogenes. Transcriptome analysis of Blimp1 CKO macrophages identified the murine chemokine (C-C motif) ligand 8, CCL8, as a direct target of Blimp1-mediated transcriptional repression in these cells. BLIMP1-deficient macrophages expressed elevated levels of Ccl8, and consequently Blimp1 CKO mice had higher levels of circulating CCL8, resulting in increased neutrophils in the peripheral blood, promoting a more aggressive antibacterial response. Mice lacking the Ccl8 gene were more susceptible to L. monocytogenes infection than were wild-type mice. Although CCL8 failed to recruit neutrophils directly, it was chemotactic for γ/δ T cells, and CCL8-responsive γ/δ T cells were enriched for IL-17F. Finally, CCL8-mediated enhanced clearance of L. monocytogenes was dependent on γ/δ T cells. Collectively, these data reveal an important role for BLIMP1 in modulating host defenses by suppressing expression of the chemokine CCL8.
    The Journal of Immunology 01/2014; 192(5). DOI:10.4049/jimmunol.1301799 · 4.92 Impact Factor

Publication Stats

30k Citations
3,150.12 Total Impact Points


  • 1995-2015
    • Massachusetts General Hospital
      • • Center for Immunology and Inflammatory Diseases
      • • Division of Infectious Diseases
      • • Pediatric Infectious Disease Unit
      • • Allergy and Clinical Immunology Unit
      • • Department of Medicine
      Boston, Massachusetts, United States
  • 1993-2015
    • Harvard Medical School
      • • Department of Microbiology and Immunobiology
      • • Department of Pathology
      • • Department of Medicine
      • • Department of Genetics
      Boston, Massachusetts, United States
  • 2000-2014
    • Harvard University
      Cambridge, Massachusetts, United States
  • 2011
      • Institute for Infection Immunology
      Hannover, Lower Saxony, Germany
  • 1997-2011
    • Howard Hughes Medical Institute
      Ashburn, Virginia, United States
  • 2007
    • Medical University of Graz
      Gratz, Styria, Austria
  • 2003
    • Lund University
      Lund, Skåne, Sweden
  • 2002
    • Harbor-UCLA Medical Center
      Torrance, California, United States
    • Dana-Farber Cancer Institute
      • Department of Pediatric Oncology
      Boston, Massachusetts, United States
    • Vanderbilt University
      • Division of Cardiovascular Medicine
      Nashville, MI, United States
  • 1999
    • Metamark Genetics
      Cambridge, Massachusetts, United States
  • 1997-1999
    • McGill University
      • Meakins-Christie Laboratories
      Montréal, Quebec, Canada
  • 1996
    • Beth Israel Deaconess Medical Center
      • Department of Medicine
      Boston, MA, United States
  • 1987-1991
    • The Rockefeller University
      • • Laboratory of Investigative Dermatology
      • • Laboratory of Cellular Physiology and Immunology
      New York, New York, United States
  • 1985-1988
    • Memorial Sloan-Kettering Cancer Center
      • DeWitt Wallace Research Laboratory
      New York, New York, United States