Alev Haliki-Uztan

Ege University, İzmir, Izmir, Turkey

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Publications (5)6.36 Total impact

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    ABSTRACT: Long-term use of prosthesis is the most important risk factor for the colonization of Candida species on the mucosal surfaces, which can lead to the development of denture-related stomatitis (DRS). Some individuals wearing prosthesis develop DRS and others do not. C. albicans strains isolated from both groups were genotypically compared. The purpose of this study was to determine whether the strain causing prosthesis stomatitis was different from the other strains genotypically. The study included 90 individuals wearing different prostheses and 20 control individuals with natural teeth. In the study 109 C. albicans strains were used which were isolated from the saliva samples and the mucosal surfaces of the tongues and palates of 51 individuals and then defined phenotypically. Phenotypic diagnosis of the isolates was genotypically verified by using species-specific PCR. For molecular typing, repetitive extragenic palindromic sequence polymerase chain reaction (REP-PCR) was employed. The results of the study revealed that REP-PCR had the capability to separate 109 C. albicans strains and six reference strains into 44 genotypes. Whereas C. albicans strains showed heterogenic distribution, C. albicans strains isolated from the individuals suffering from prosthesis stomatitis showed no specific genotypes. REP-PCR is a simple, fast and low-cost method and helped work on a great number of samples.
    European Journal of Clinical Microbiology 09/2010; 30(2):141-9. · 3.02 Impact Factor
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    ABSTRACT: The aim of this study was to determine Candida spp. incidence in the oral cavity of denture wearers and characterize predisposing factors in denture-related stomatitis (DRS). Three groups of denture wearers and a control group were evaluated for DRS according to Newton's classification. The amount of yeast in saliva and the presence of yeast on mucosal surfaces were determined by phenotyping methods, and the impact of some risk factors on candidal carriage was evaluated. The development of DRS is most common in complete prosthesis users. When the count of yeast in saliva is >or=400 cfu/ml, the frequency of DRS is increased. In individuals who develop DRS, the most frequently encountered species that was identified as C. albicans. Prosthetic hygiene was related to the intensity of candidal growth and the development of DRS. C. albicans live as saprophyte in the oral cavity. But, it is capable of causing infection if there are predisposing conditions related to the host. Usage of removable prosthesis may cause these microorganisms to gain pathogenicity.
    Mycopathologia 02/2010; 169(5):365-72. · 1.65 Impact Factor
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    ABSTRACT: In this research, in order to determine mean fungus counts, indoor and outdoor air samples were taken in five elementary school buildings located in the city center of Seferihisar, Izmir (45 km from Izmir) within a 1-year period between March and April 2004, and between January and February 2005 representing similar climatic characteristics. Five samples, three from three classes where 5-8, 10-12, and 12-15 age group students attended, one from the corridors, and one from outside the buildings in all the schools, were taken for each period. Within the period of the study, in indoor and outdoor air samples, 7,122 microfungus colonies were counted. Sixty-four species were identified that belong to 17 genera as a result of the identification of isolates obtained. Skin prick tests were applied to 55 out of a total of 117 teachers by the clinicians. According to these test results, 24 teachers were sensitive to at least one agent. Results revealed that, in terms of mold counts, the difference between the schools and the difference between the times (periods) were statistically significant (p < 0.05).
    Environmental Monitoring and Assessment 09/2009; 168(1-4):691-702. · 1.68 Impact Factor
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    Ozlem Abaci, Alev Haliki-Uztan
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    ABSTRACT: The aim of this study was to determine the the prevalence of in vitro resistance amongst Candida species isolated from the oral cavity of denture wearers. The in vitro susceptibility of 156 Candida isolates to amphotericin B, fluconazole, 5-fluorocytosine, caspofungin and terbinafine was determined. The Clinical Laboratory Standards Institute' (CLSI; formally National Committee for Clinical Laboratory Standards) broth microdilution method was used and MIC 50 and MIC 90 determined. Candida albicans, the most frequently isolated strains, are sensitive to amphotericin (61%) and fluconazole (44%), frequently used agents in the treatment of Candida-associated denture stomatitis. A 100% susceptibility to 5-fluorocytosine was observed among the 109 isolates of C. albicans. Among non C. albicans strains only 1 Candida kefyr strain was determined as susceptible dependent upon dose for 5-fluorocytosine. Among Candida glabrata, the second most common isolate, a 100% susceptibility to caspofungin and 5-fluorocytosine were observed. Since the isolates are sensitive to Caspofungin and 5-fluorocytosine, rarely used in the treatment of oral fungal infections, it is suggested that these antifungal agents be used as alternative medicine in the treatment of oral infections especially caused by strains resistant to amphotericin B and fluconazole. INTRODUCTON Since Candida species, which are among the normal flora microorganisms of human body, are opportunist pathogens, they can cause different clinical manifestations of candidiasis (Scardina et al., 2007). Oral candidiasis is the most common mycotic infection in the oral cavities in humans. Long-term use of prosthesis is the most important risk factor for Candida species colonization of the mucosal surfaces, this may be sufficient for the development of oral candidiasis. Oral fungal infection that causes Candida associated prosthesis stomatitis is a common disease in 50 to 60% of denture wearers (Budtz-Jorgensen, 2000; Darwazeh et al., 2001; Pıres et al., 2002).
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    Ayşegül Yoltaş, Alev Haliki-Uztan
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    ABSTRACT: Giriş Ev, okul, işyeri gibi çevrelerdeki kimyasal ve biyolojik ajanların potansiyel tehlikeleri hakkında bilgi sahibi olunmasıyla iç alanlarda mikotoksin ve mikotoksin üreticisi küflerin varlığının araştırılması büyük önem kazanmıştır. Son yıllarda hava kaynaklı küfler ve biyolojik kontaminatların potansiyel sağlık etkileri IAQ (International Air Quality) araştırmalarında ciddi bir şekilde göz önünde tutulan bir faktördür (1, 2). İç alan havasında bulunan funguslar insan sağlığını alerjik, infeksiyöz ve toksik yollar ile etkilemektedirler (3). Medya ve halkın iç alanlarda bulunan küflere karşı ilgisinin artmasıyla endüstriyel hijyen uzmanları daha çok aranır hale gelmişlerdir. Endüstriyel hijyen uzmanlarından ortamlardaki küf varlığının incelenmesi ve bunlardan kurtulma yöntemleri hakkında öneri vermeleri istenmektedir. Bu öneriler yapıldığında "eğer küf kontaminasyonu ortadan kaldırılmasa ciddi sağlık sorunları ortaya çıkabilir" gibi bir kanı oluşur. Toksik küfler hakkındaki haberler giderek mikotoksin üreticisi olduğu bilinen belirli küf türlerine karşı ilgi ve endişeyi de artırmıştır. Bu konuda özellikle Stachybotrys chartarum en çok ilgi çeken tür olmuştur. Küf ve mikotoksinler için belirli maruz kalma standartları bulunmadığından endüstriyel hijyen uzmanları iç çevrelerdeki küflerle ilişkili sağlık etkileri ve bu küflere maruz kalma potansiyeli hakkında geniş bilgiye sahip olmalıdır (2, 3). Mikotoksinlerin solunması konusundaki nicel risk yargılarının tam olarak oluşturulabilmesi için gerekli bilgilerde büyük eksikler bulunmaktadır. Mikotoksinlerin hayvanlara injeksiyonunu içeren çok sayıda kaynak bulunmasına rağmen mikotoksinlerin solunması hakkında yapılmış çok az çalışma vardır. Buna ek olarak, yapılmış inhalasyon toksisitesi çalışmaları arasında bazı uyuşmazlıklar da gözlenmiştir. Bazı çalışmalarda mikotoksinlerin solunarak alınmasının diğer maruz kalma yollarına nazaran daha riskli olduğu söylenmekte bazılarında ise mikotoksinlerin solunmasına bu kadar önem verilmemektedir (3).