Xiangmin Zhang

Fudan University, Shanghai, Shanghai Shi, China

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Publications (211)762.39 Total impact

  • Ya Xiong, Chunhui Deng, Xiangmin Zhang
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    ABSTRACT: Herein, we developed a novel method based on aptamer-conjugated magnetic graphene/gold nanoparticles nanocomposites (MagG@Au) for specific enrichment and rapid analysis of thrombin in biological samples using MALDI-TOF-MS.
    Talanta 11/2014; 129:282–289. · 3.51 Impact Factor
  • Qi Chen, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
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    ABSTRACT: To analyze the proteome of an extremely low number of cells or even a single cell, we established a new method of digesting whole cells into mass-spectrometry-identifiable peptides in a single step within 2 h. Our sampling method greatly simplified the processes of cell lysis, protein extraction, protein purification, and overnight digestion, without compromising efficiency. We used our method to digest hundred-scale cells. As far as we know, there is no report of proteome analysis starting directly with as few as 100 cells. We identified an average of 109 proteins from 100 cells, and with three replicates, the number of proteins rose to 204. Good reproducibility was achieved, showing stability and reliability of the method. Gene Ontology analysis revealed that proteins in different cellular compartments were well represented.
    Analytical and Bioanalytical Chemistry 10/2014; · 3.66 Impact Factor
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    ABSTRACT: A rapid and accurate in-vitro drug metabolism strategy has been proposed based on the design of a biomimetic nanoreactor composed of amino-functionalized periodic mesoporous organosilica (NH2-PMO) and microsomes. The amphiphilic and positive charged NH2-PMO makes it highly suited for the immobilization of hydrophobic and negatively charged microsomes to form nanoreactors, which can in turn extract substrates from solutions. Such nanoreactors provide a suitable environment to confine multiple enzymes and substrates with high local concentrations, as well as to maintain their catalytic activities for rapid and highly effective drug metabolic reactions. Coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis, the metabolites of nifedipine and testosterone were characterized and the reaction kinetics was quantitatively evaluated. Both the metabolism conversion and reaction rate were significantly improved with the NH2-PMO nanoreactors compared to bulk reactions. This strategy is simple and cost-effective for promising advances in biomimetic metabolism study.
    Analytical Chemistry 10/2014; · 5.83 Impact Factor
  • Chenyi Shi, Chunhui Deng, ShiEn Zou, Xiangmin Zhang
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    ABSTRACT: Mass spectrometric technique has emerged as a preferred technique in the analysis of protein phosphorylation. Owing to the low stoichiometry of phosphopeptides and the signal suppression effect by non-phosphopeptides, there is a demand for efficient enrichment of phosphopeptides. The selective enrichment of phosphopeptides in modified eppendorf tubes prior to mass spectrometry analysis, which can minimize sample loss as well as nonspecific interferences effectively, has become a hot topic in current proteomics field. In our work, an easy-to-use phosphopeptide-selective eppendorf tube was initially prepared, with its inner surface being modified with a Ti(4+)-immobilized polydopamine (PDA) layer. The unique Ti(4+)-immobilized PDA-modified eppendorf tubes (EP tube@PDA-Ti(4+)) are investigated for its application in selective enrichment of phosphopeptides from complex biological samples. Due to the high Ti(4+) loading amount on the surface of PDA, the EP tube@PDA-Ti(4+) exhibits remarkable phosphopeptide enrichment ability in protein digests and human serum, which presents a powerful evidence for its high selectivity in detecting the low-abundance phosphopeptides from complex biological samples.
    Talanta 09/2014; 127:88–93. · 3.50 Impact Factor
  • Man Zhao, Yiqin Xie, Chunhui Deng, Xiangmin Zhang
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    ABSTRACT: Many endogenous proteins/peptides and proteins/peptides with post-translational modifications (PTMs) are presented at extremely low abundance, and they usually suffer strong interference with highly abundant proteins/peptides as well as other contaminants, resulting in low ionization efficiency in MS analysis. Therefore, the separation and enrichment of proteins/peptides from complex mixtures is of great importance to the successful identification of them. Core-shell structured magnetic microspheres have been widely used in the enrichment and isolation of proteins/peptides, thanks to unique properties such as strong magnetic responsiveness, outstanding binding capacity, excellent biocompatibility, robust mechanical strength and admirable recoverability. The aim of this review is to update the advances in the application of core-shell structured magnetic materials for proteomics analysis, including the separation and enrichment of low-concentration proteins/peptides, the selective enrichment of phosphoproteins and the selective enrichment of glycoproteins, and to compare the enrichment performance of magnetic microspheres with different kinds of functionalization
    Journal of Chromatography A 08/2014; · 4.26 Impact Factor
  • Chunhui Deng, Xiangmin Zhang, Danni Huang
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    ABSTRACT: Functionalized magnetic nanomaterials, composed of both organic and inorganic components, have recently been identified as promising solid-phase extraction adsorbents for various applications. Due to their superparamagnetic property, large specific surface area, and selective adsorption capacity, this unique class of nanomaterials exhibits an excellent performance in extracting and enriching numerous targeted analytes. This review mainly focuses on the recent advances in the synthesis and application of functionalized magnetic nanomaterials for the preconcentration of organic pollutants in environmental analysis. The development of three kinds of magnetic solid-phase adsorbents, including magnetic polymer nanomaterials, magnetic hybrid nanomaterials and magnetic mesoporous nanomaterials are elaborated in this review.
    Analytical methods 07/2014; · 1.94 Impact Factor
  • Nianrong Sun, Chun-Hui Deng, Yan Li, Xiangmin Zhang
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    ABSTRACT: Developing an effective separation method is necessary for identifying low-abundant endogenous phosphorylated peptides with the removal of proteins. In this work, we prepared size-exclusive magnetic graphene /mesoporous silica composites with titanium (Ⅳ)-immobilized pore wall (denoted as Ti4+- MGMSs) for capturing endogenous phosphorylated peptides for mass spectrometry analysis. The introduction of hydrophilic polydopamine simplified the synthetic process of Ti4+- MGMSs and the ordered mesoporous channels are beneficial to trap the endogenous phosphopeptides while large-size protein excluded. Furthermore, the magnetic performance simplifies the entire process of the enrichment greatly. With all the advances, the novel Ti4+-MGMSs present high enrichment efficiency either from the low concentration of β-casein tryptic digest (0.5 fmol/μL) or the mixture of β-casein tryptic digest and α-casein (or plus BSA, with the mass ratio up to 1:500). Besides, Ti4+- MGMSs have also been successfully applied to enrich endogenous phosphorylated peptides from human serum and human saliva.
    ACS Applied Materials & Interfaces 07/2014; · 5.90 Impact Factor
  • Mengyi Wang, Chun-Hui Deng, Yan Li, Xiangmin Zhang
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    ABSTRACT: In this work, for the first time, binary metal oxides ((Ti-Sn)O4) were integrated into one entity on an atomic scale on magnetic graphene as affinity probe for highly selective enrichment of phosphopeptides. The newly prepared Fe3O4/graphene/(Ti-Sn)O4 (magG/(Ti-Sn)O4) composites gathered the advantages of large specific surface area of graphene, super-paramagnetism and biocompatibility of iron oxide, and enhanced affinity properties of binary metal oxides. The phosphopeptide enrichment efficiency of the magG/(Ti-Sn)O4 composite was investigated, and the results indicated an ultra-low detection limit (1 pg/μL or 4.0×10-11 M) and an ultra-high selectivity (weight ratio of β-casein and BSA reached up to 1:1500). Compared with magnetic affinity probes with single metal oxide (magG/TiO2, magG/SnO2) or the simple physical mixture of magG/TiO2 and magG/SnO2, the magG/(Ti-Sn)O4 composite possessed stronger specificity, higher selectivity and better efficiency; and more importantly, it possessed the ability to enrich both the mono- and multi- phosophorylated peptides, demonstrating the notable features of the novel binary metal oxides affinity probe in the specific and selective enrichment of phosphopeptides. Additionally, by utilizing the magG/(Ti-Sn)O4 composites, a total number of 349 phosphorylation sites on 170 phosphopeptides including 66 mono-phosphopeptides and 104 multi-phosphopeptides were captured and identified from mouse brain, indicating the great potential for their application in phosphoproteomics analysis in the future.
    ACS Applied Materials & Interfaces 06/2014; · 5.90 Impact Factor
  • Man Zhao, Chunhui Deng, Xiangmin Zhang
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    ABSTRACT: In this work, polydopamine (PDA)-coated magnetic microspheres with surface modification of zirconium-based MOFs were synthesized for the first time. The as-synthesized Fe3O4@PDA@Zr-MOF composites were successfully applied as a novel immobilized metal ion affinity platform for phosphoproteome research.
    Chemical Communications 04/2014; · 6.38 Impact Factor
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    ABSTRACT: In this work, a facile route was initially developed for preparation of a novel metal oxide affinity chromatography (MOAC) material by grafting titania nanoparticles on polydopamine (PD)-coated graphene (denoted as G@PD@TiO2). In the first step, self-assemble polymerization of dopamine on graphene was performed in basic solution at room temperature, which not only offered the coupling linker between titania and graphene but also improved the hydrophilicity and biological compatibility of the nanohybrids. Thereafter, the titania nanoparticles were grafted on the surface of the PD-coated graphene via a simple hydrothermal treatment. The as-prepared G@PD@TiO2 nanohybrids exhibited high sensitivity (detection limit of 5 fmol) and high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonphosphopeptides (1:1000). Moreover, the as-prepared nanohybrids were also investigated for enrichment of phosphopeptides from real biological samples (human serum and mouse brain). A total number of 556 phosphorylation sites were identified from the digest of mouse brain proteins, showing great potential in the practical application.
    Analytical Chemistry 04/2014; 86(9):4327–4332. · 5.83 Impact Factor
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    ABSTRACT: Human plasma is one of the proteins-containing samples most difficult to characterize on account of the wide dynamic concentration range of its intact proteins. Herein, we developed a high-throughput conventional array-based two-dimensional liquid chromatographic system for proteins separation in human plasma in online mode. In the system, a conventional strong-anion exchange chromatographic column was used as the first separation dimension and eight parallel conventional reversed-phase liquid chromatographic columns were integrated as the second separation dimension. The fractions from the first dimension were sequentially transferred into the corresponding reversed-phase liquid chromatographic precolumns for retention and enrichment using a 10-port electrically actuated multi-position valve. The second dimensional solvent flow was directly and identically split into 8 channels. The fractions were concurrently back-flushed from the precolumns into the 8 conventional RP columns and were separated simultaneously. An 8-channel fraction collector was refitted to collect the reversed-phase liquid chromatographic fractions for further investigation. Bicinchoninic acid (BCA) dyein solution was conveniently used for high-abundance protein location. Two separation dimensions were relatively independent parts, as well as each channel of the second dimensional array separation. Therefore, the new system could improve the separation throughput and total peak capacity. The system was successfully applied for the separation of human plasma intact proteins. The results indicated the established system is an effective method for removing high abundance proteins in plasma and in-depth research in plasma proteomics.
    04/2014; 32(4):343-8.
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    ABSTRACT: In this work, polydopamine-coated magnetic graphene (MG@PDA) nanocomposites were synthesized by a facile method. Trypsin was then directly immobilized on the surface of the nanocomposites through simple PDA chemistry with no need for introducing any other coupling groups. The as-made MG@PDA nanocomposites inherit not only the large surface area of graphene which makes them capable of immobilizing high amount of trypsin (up to 0.254 mg/mg), but also the good hydrophilicity of PDA which greatly improves their biocompatibility. Moreover, the strong magnetic responsibility makes them easy to be separated from the digested peptide solution when applying a magnetic field. The feasibility of the trypsin-immobilized MG@PDA (MG@PDA-trypsin) nanocomposites for protein digestion was investigated and the results indicated their high digestion efficiency in a short digestion time (10 min). In addition, the reusability and stability of the MG@PDA-trypsin nanocomposites were also tested in our work. To further confirm the efficiency of MG@PDA-trypsin nanocomposites for proteome analysis, they were applied to digest proteins extracted from skimmed milk, followed by nano RPLC-ESI-MS/MS analysis, and totally 321 proteins were identified, much more than those obtained by 16 h in-solution digestion (264 proteins), indicating the great potential of MG@PDA-trypsin nanocomposites as the supports for high throughput proteome study.This article is protected by copyright. All rights reserved
    Proteomics 04/2014; · 3.97 Impact Factor
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    ABSTRACT: In this work, a facile route was initially developed for preparation of a novel MOAC material by grafting titania nanoparticles on polydopamine (PD) coated graphene (denoted as G@PD@TiO2). In the first step, self-assemble polymerization of dopamine on graphene was performed in basic solution at room temperature, which not only offered the coupling linker between titania and graphene but also improved the hydrophilicity and biological compatibility of the nanohybrids. Thereafter, the titania nanoparticles were grafted on the surface of the PD coated graphene via a simple hydrothermal treatment. The as-prepared G@PD@TiO2 nanohybrids exhibited high sensitivity (detection limit of 5 fmol) and high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonphosphopeptides (1:1000). Moreover, the as-prepared nanohybrids were also investigated for enrichment of phosphopeptides from real biological samples (human serum and mouse brain). A total number of 556 phosphorylation sites were identified from the digest of mouse brain proteins, showing great potential in the practical application.
    Analytical Chemistry 03/2014; · 5.83 Impact Factor
  • Yinghua Yan, Xiangmin Zhang, Chun-Hui Deng
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    ABSTRACT: Metal oxide affinity chromatography (MOAC) is a powerful technique in phosphoproteome research. However, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by MOAC remains a big challenge since the lack of high specificity and large binding capacity of conventional MOAC materials. In this work, a new MOAC material, TiO2-coated hierarchically ordered macro/mesoporous silica (denotes as HOMMS@TiO2) composites were prepared via a facile process. The HOMMS@TiO2 composites were demonstrated to have low limit of detection (8 fmol) and great specificity with a very rapid enrichment speed (within 1 min). These experiment results have demonstrated that the HOMMS@TiO2 exhibit great potential in phosphoproteome research.
    ACS Applied Materials & Interfaces 03/2014; · 5.90 Impact Factor
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    ABSTRACT: Feasible design is essential to achieve ideal chemical and biological properties of nanomaterials. For the first time, new nanocomposites with a polydopamine coating on hierarchically ordered macro-/mesoporous silica functionalized with titanium ions (denoted as HOMMS-PD-Ti4+) were prepared through a facile reaction route at room temperature. The applicability of as-synthesized HOMMS-PD-Ti4+ for the selective enrichment of phosphopeptides was tested. The experimental results demonstrate that, by taking advantage of the pure phosphate–Ti4+ interface and high loading amount of Ti4+, HOMMS-PD-Ti4+ presents remarkable selectivity for phosphopeptides, even at a low molar ratio of phosphopeptides/non-phosphopeptides (1:1000) with a very rapid enrichment speed (within 1 min). The superior sensitivity for low-abundant phosphopeptides and the high selectivity and effectiveness for the enrichment of phosphopeptides from human serum are also proven. These outstanding features demonstrate that HOMMS-PD-Ti4+ exhibits great potential in phosphoproteome research in the future.
    ChemPlusChem 03/2014; · 3.24 Impact Factor
  • Nianrong Sun, Chun-Hui Deng, Yan Li, Xiangmin Zhang
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    ABSTRACT: Abnormal protein glycosylation has been demonstrated to be associated with many diseases,therefore,it is very important to conduct a comprehensive structure analysis of glycan for prognosis and diagnosis of the disease, such as cancer. In this work, for the first time, carbon-functionalized ordered graphene/mesoporous silica composites (denoted as C-graphene@mSiO2) with large surface area and uniform pore size were designed and synthesized. By taking advantage of the special interaction between the carbon and glycans as well as size-exclusion ability, 25 N-linked glycan released from ovalbumin could be observed clearly with strong MS signals and increased signal-to-noise (S/N) ratio. In addition, after enrichment with the C-graphene@mSiO2 composites, 48 N-linked glycans (S/N>10) with sufficient peak intensities could be obtained from only 400 nL of the healthy pristine human serum. The facile and low-cost synthesis method as well as high selective enrichment ability of the novel C-graphene@mSiO2 composite makes it a promising tool for the glycosylation research.
    Analytical Chemistry 01/2014; · 5.83 Impact Factor
  • Man Zhao, Chunhui Deng, Xiangmin Zhang
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    ABSTRACT: For the first time, C8-functionalized magnetic graphene (magG) has been designed and synthesized with a polydopamine (PDA) coating. The magG prepared by a solvothermal reaction was encapsulated in a layer of PDA through the oxidative polymerization of dopamine in alkaline buffer, and C8 groups were grafted onto magG/PDA composites through a silanization method. The as-prepared material integrates the merits of graphene, magnetic microspheres, PDA, and C8 chains; thus possessing an ultrahigh specific area, strong magnetic responsiveness, excellent solubility, and an extraordinary enrichment capability. The C8-functionalized magnetic composites were employed in the enrichment and identification of low-concentration standard peptides, peptides in standard protein digest solutions, and endogenous peptides in human urine. The enriched peptides were eluted and analyzed by MALDI-TOF MS. The MS results indicated that the C8-functionalized material exhibited the distinguished ability to enrich hydrophobic peptides mainly through hydrophobic–hydrophobic interactions. Moreover, thanks to the enrichment approach based on magG@PDA@C8, the limit of detection of the standard peptide decreased to as low as 50 pM. The experimental results demonstrate that the magG@PDA@C8 composite is a promising candidate for the enrichment of low-abundance peptides in biological samples.
    ChemPlusChem 01/2014; · 3.24 Impact Factor
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    ABSTRACT: Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO2 composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO2 material demonstrated low limit of detection (20fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein β-casein with molar ratios of BSA/β-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO2 composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis.
    Talanta 01/2014; 118:14-20. · 3.50 Impact Factor
  • Chaofeng Wang, Mingxia Gao, Zhi Huang, Xiangmin Zhang
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    ABSTRACT: The new approach to one-step derivatization of saccharide with 5-(((2-(carbohydrazino)methyl)thio)acetyl)-aminofluorescein (C356) was described. In this approach, high fluorescent C356 was applied to label saccharide to enhance the response of derivative saccharide and high sensitive capillary high performance liquid chromatography with laser-induced fluorescence (Capillary-HPLC-LIF) associated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to characterize C356 labeled saccharide. The effect of derivatization conditions was evaluated and discussed. The limit of detection (LOD) of neutral saccharide in our method attained the level of femtomolar. As a result, this method could be successfully applied to determine the structure of N-glycans of glycoprotein.
    Talanta 12/2013; 117C:229-234. · 3.50 Impact Factor
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    ABSTRACT: In this study, novel raisin-bread sandwich-structured magnetic graphene/mesoporous silica composites with C18-modified interior pore-walls (mag-graphene@mSiO2-C18) were synthesized by coating mesoporous silica layers onto each side of magnetic graphene through a surfactant-mediated co-condensation sol-gel process. The prepared functionalized nanocomposites possessed marvelous properties of extended plate-like morphology, fine water dispersibility, high magnetic response, large surface area (315.4cm(2)g(-1)), uniform pore size (3.3nm) and C18-modified interior pore-walls. Several kinds of phthalates were selected as model analytes to systematically evaluate the performance of adsorbents in extracting hydrophobic molecules followed by gas chromatography-mass spectrometry analyses. Various extraction parameters, including pH value of sample solution, amounts of adsorbents, adsorption time, species and volume of eluting solvent, and desorption time were optimized. The anti-interference ability to macromolecular proteins was also investigated. Method validations such as linearity, recovery, reproducibility, and limit of detection were also studied. Finally, mag-graphene@mSiO2-C18 composites were successfully applied to analyzing phthalates in environmental water samples. The results indicated that this novel approach offered an attractive alternative for rapid, convenient, efficient and selective magnetic solid-phase extraction for targeted hydrophobic compounds.
    Journal of Chromatography A 12/2013; · 4.61 Impact Factor

Publication Stats

3k Citations
762.39 Total Impact Points

Institutions

  • 2001–2014
    • Fudan University
      • • Department of Chemistry
      • • Department of Macromolecular Science
      Shanghai, Shanghai Shi, China
  • 2012
    • Shenyang Ligong University
      Feng-t’ien, Liaoning, China
  • 2009
    • Shanghai Institutes for Biological Sciences
      Shanghai, Shanghai Shi, China
  • 2007
    • Second Military Medical University, Shanghai
      Shanghai, Shanghai Shi, China