H Isono

Gifu University, Gihu, Gifu, Japan

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Publications (72)52.5 Total impact

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    ABSTRACT: Tibetan medicated-bath therapy has been applied to patients with rheumatoid arthritis for centuries. However, the detailed action mechanism of Tibetan medicated-bath therapy on the morphology and function of joints remains unknown. We designed our investigation to evaluate the efficacy of Tibetan medicated-bath therapy on adjuvant arthritis (AA) of rats in comparison with water-bath and dexamethasone administration. AA was induced by intradermal injection of Mycobacterium butyricum suspended in sterile mineral oil. The control animals were similarly injected with sterile vehicle. Eight days after injection, rats were treated with fresh-water bath, Tibetan medicated-bath (40 degrees C, 15 min) or intramuscular injection with dexamethasone for 21 consecutive days after which we evaluated the severity of arthritis visually and microscopically and measured serum interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha levels. While arthritis did not significantly change after water-bath treatment, the Tibetan medicated-bath and dexamethasone groups showed diminished joint swelling and alleviation of, inflammatory cell infiltration and the destruction of bone and cartilage. Serum IL-6 and TNF-alpha levels significantly decreased. Our results demonstrated that Tibetan medicated-bath therapy exerted a reliable effect on rat adjuvant arthritis, which may be involved in the inflammatory cytokines, IL-6 and TNF-alpha. Our data provide evidence for clinical use of Tibetan-medicated bath therapy for arthritis patients.
    Evidence-based Complementary and Alternative Medicine 12/2007; 6(2):211-7. · 1.72 Impact Factor
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    ABSTRACT: We evaluated the effects of the traditional Chinese medicines, Hachimi-jio-gan, Juzen-taiho-to and Unkei-to, on bone loss in murine model of senile osteoporosis (SAMP6). Two-month-old SAMP6 were divided into control and experimental groups. The control mice had the tap water available as the only drinking fluid. The experimental mice were given 0.05% aqueous solution of Hachimi-jio-gan, Juzen-taiho-to or Unkei-to for three months. The solution intake of a mouse averaged 5 ml per day. The bones were studied morphologically and histomorphometrically, together with bone mineral density (BMD), serum parathyroid hormone (PTH) and estradiol levels. In the control group, BMD and the amount of bone forming surface were low, the serum PTH level was high when compared with the normal mice SAMR1. Many osteocytes and osteoblasts showed degenerative changes and numerous mast cells were observed in the bone marrow. Compared with controls, the serum estradiol level was higher in the Unkei-to group. However, we did not find any significant changes of bones. In the Hachimi-jio-gan and Juzen-taiho-to groups, the bone mass and the amount of bone forming surface increased. Most of the osteocytes and osteoblasts appeared normal. As compared with controls, the number of mast cells in bone marrow decreased in the Hachimi-jio-gan group. The serum PTH level had declined in the Juzen-taiho-to group. The present study provides certain evidence that Hachimi-jio-gan and Juzen-taiho-to are effective in preventing bone loss in SAMP6, while Unkei-to can only improve the ovary function.
    Biological & Pharmaceutical Bulletin 06/2005; 28(5):865-9. · 1.85 Impact Factor
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    ABSTRACT: The ultrastructure of the parathyroid glands of adult Japanese lizards (Takydromus tachydromoides) in the spring and summer season was examined. The parenchyma of the gland consists of chief cells arranged in cords or solid masses. Many chief cells contain numerous free ribosomes and mitochondria, well-developed Golgi complexes, a few lysosome-like bodies, some multivesicular bodies and relatively numerous lipid droplets. The endoplasmic reticulum is mainly smooth-surfaced. Cisternae of the rough endoplasmic reticulum are distributed randomly in the cytoplasm. Small coated vesicles of 700-800 Å in diameter are found occasionally in the cytoplasm, especially in the Golgi region. The chief cells contain occasional secretory granules of 150-300 nm in diameter that are distributed randomly in the cytoplasm and lie close to the plasma membrane. Electron dense material similar to the contents of the secretory granules is observed in the enlarged intercellular space. These findings suggest that the secretory granules may be discharged into the intercellular space by an eruptocrine type of secretion. Coated vesicles (invaginations) connected to the plasma membrane and smooth vesicles arranged in a row near the plasma membrane are observed. It is suggested that such coated vesicles may take up extracellular proteins. The accumulation of microfilaments is sometimes recognized. Morphological evidence of synthetic and secretory activities in the chief cells suggests active parathyroid function in the Japanese lizard during the spring and summer season.
    Journal of Morphology 02/2005; 161(2):145 - 155. · 1.60 Impact Factor
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    ABSTRACT: In this study, the preventive effects of the traditional Chinese medicine, Hachimi-jio-gan, Juzen-taiho-to and Unkei-to were investigated, on bone loss in the murine model of senile osteoporosis, senescence-accelerated mouse P6 (SAMP6). The experimental animals were given 0.05% solution of the Chinese medicines for 3 months. Bone mineral density and bone morphology were studied together with serum parathyroid hormone and estradiol levels. After the treatment with Unkei-to, the serum estradiol level was increased without any marked change in the bone. Bone mass was significantly increased after the treatment with Hachimi-jio-gan or Juzen-taiho-to. These findings suggest that Hachimi-jio-gan and Juzen-taiho-are effective in preventing bone loss in SAMP6.
    Geriatrics and Gerontology International 01/2004; 4. · 2.17 Impact Factor
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    ABSTRACT: We studied the ultrastructure of the parathyroid gland of young golden hamsters after short-term treatment with ethanol (1.5 g/kg bw or 6.0 g/kg bw). We did not find any ultrastructural changes of the parathyroid gland after administration of 1.5 g/kg ethanol. In the hamsters, 3 hours after administration of 6.0 g/kg ethanol, the mean serum calcium concentration was significantly low as compared to that of the control animals. In the parathyroid gland 1 hour after administration of 6.0 g/kg ethanol, the Golgi complexes associated with a few prosecretory granules and the volume density occupied by the Golgi complexes decreased compared with that of the control animals. In the parathyroid glands 3 hours after administration of 6.0 g/kg ethanol, the Golgi complexes decreased as compared with those of the control animals, while the large vacuolar bodies increased. These findings suggest that the cellular activity of the parathyroid gland is suppressed after short-term treatment with ethanol. Intracellular lumen was found in the parathyroid chief cells 3 hours after administration of 6.0 g/kg ethanol, and the significance of this structure is discussed.
    Histology and histopathology 11/1998; 13(4):973-9. · 2.28 Impact Factor
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    ABSTRACT: Although there have been many reports regarding the structure of the parathyroid glands of various terrestrial mammals, little is known about the parathyroid glands of marine mammals including Cetacea. The morphology of the parathyroid glands of three Risso's dolphins,Grampus griseus(about 3 m in length and 300 kg in weight), and three bottlenose dolphins,Tursiops truncatus(about 3 m in length and 300 kg in weight), was examined macroscopically and microscopically. The dolphins examined in the present study had two or four parathyroid glands that varied in size and location on the thyroid gland. Each parathyroid gland was encapsulated by fibrous tissue on the dorsal surface of the thyroid gland, and was divided into several lobules by interlobular connective tissue which contained numerous capillaries. The parenchymal cells consisted of pale staining chief cells. Each cell was polygonal and about 15 μm in diameter, and had one round or oval nucleus. Oxyphil cells were not found. Considering their greater body size, the parathyroid glands were rather small. By electron microscopic observation, the parathyroid gland of the bottlenose dolphin had sparse granular endoplasmic reticulum, poorly developed Golgi complexes, and abundant secretory granules in the cytoplasm of the chief cells. These results support a possibility that the activity of the parathyroid gland is suppressed to adapt to a sea habitat.
    General and Comparative Endocrinology 04/1998; · 2.82 Impact Factor
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    ABSTRACT: To investigate the morphological changes of the parathyroid gland of the immobilized hamsters, we studied the ultrastructure of the parathyroid gland of golden hamsters kept in special small cage (Ballman cage II). All hamsters of the control group were kept in one ordinary cage. Each hamster of the isolated group was kept in ordinary cage individually. Each hamster of the immobilized group was kept in Ballman cage II individually. All hamsters were kept for 5 days. On the first and fifth day of the experiment, bone mineral content (BMC) and bone mineral density (BMD) of whole body were measured by dual energy X-ray absorptiometry (DXA). In the control and isolated groups, BMD of the fifth day was significantly increased as compared to that of the first day. In the immobilized group BMC and body weight were significantly decreased. There was no significant difference among 3 groups concerning the mean serum calcium level. Volume density of the cell organelles and inclusions was estimated and compared among 3 groups. Volume density of the lysosomes and large vacuolar bodies of the isolated and immobilized groups was significantly higher than that of the control group. Much more lipid droplets were observed in the immobilized group than the control and isolated groups. No particular differences were observed as to the Golgi complex in the isolated and the immobilized groups compared to the control group. These findings suggest that the cellular activity of the parathyroid gland is suppressed with immobilization.
    Okajimas Folia Anatomica Japonica 04/1998; 74(6):259-69.
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    ABSTRACT: The ultrastructure of the parathyroid glands (PTG) of magnesium (Mg)-treated golden hamsters exposed to centrifugation at 1.2 G for 7 days was compared to that of the control, Mg-treated or centrifuged groups. In the PTG of the Mg-treated group exposed to centrifugation, the Golgi apparatus increased compared with that of the Mg-treated group, but was similar to that of the control and centrifuged groups. The cisternae of the rough endoplasmic reticulum (rER) increased compared with that of the Mg-treated group and decreased compared with that of the centrifuged group, but was the same as that of the control group. There were no significant differences among the 4 groups with regard to lysosomes, large vacuolar bodies, lipid droplets and secretory granules. These observations suggest that the rER is sensitive to centrifugation compared with the Golgi apparatus and more importantly the inhibitive effect of Mg on the synthesis of the hamster parathyroid hormone is enfeebled by centrifugation at 1.2 G.
    Okajimas Folia Anatomica Japonica 11/1997; 74(4):125-31.
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    ABSTRACT: Lung piece obtained from a female patient with psittacosis by biopsy. A nuclear bodies of five different types were observed, depending on their degree of ultrastructural development. First type showed fibrillary nuclear bodies surrounded by a clear halo. Type II resembled the nuclear body of type I but showed heterogeneously electron density. Type III comprised nuclear bodies with a fibrillary cortex containing a central granular component. These bodies were separated from the nucleoplasm by a clear halo. Type IV was characterized by a more complex structure and resembled the nuclear body of type III but with dense osmiophilic granules. Nuclear bodies of type V were comprised of numerous osmiophilic granules.
    Okajimas Folia Anatomica Japonica 11/1997; 74(4):139-45.
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    ABSTRACT: We examined the effect of magnesium (Mg) on the fine structure of the golden hamster parathyroid gland in vivo and in vitro. In the in vivo study, the principal changes in the parathyroid glands 10 and 30 min, and 1 hr after Mg injection showed a significant decrease in the area of the Golgi apparatus and cisternae of the rough endoplasmic reticulum compared with those of the control animals. The serum calcium level in the experimental animals 30 min and 1 hr after the injection was significantly low compared with that of the control animals. In the vitro study, the area of the Golgi apparatus in the Mg-treated group significantly decreased as compared with that of the control group. These changes suggest that Mg directly suppresses the synthesis of hamster parathyroid hormone in the short term.
    Okajimas Folia Anatomica Japonica 09/1997; 74(2-3):81-91.
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    ABSTRACT: The large vacuolar bodies in the parathyroid glands of pregnant golden hamsters after administration of CaCl2 or EDTA were investigated. In the parathyroid glands of the pregnant animals 15 min after administration of CaCl2, the mean serum calcium concentration was significantly high when compared to that of the control animals. In the parathyroid glands of the pregnant animals 15, 30 and 60 min after administration of EDTA, the mean serum calcium concentration was significantly low when compared to that of the control animals. In the parathyroid glands of the pregnant animals 15 min after administration of CaCl2, the percentage area occupied by large vacuolar bodies was significantly increased when compared to that of the control animals. In the parathyroid glands of the pregnant animals 15 min after administration of EDTA, the percentage area occupied by large vacuolar bodies was significantly decreased when compared to that of the control animals.
    Histology and histopathology 08/1997; 12(3):617-21. · 2.28 Impact Factor
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    ABSTRACT: This anatomical study concerns the arterial supply of the human acetabulum. The purpose is to contribute to prevention of postoperative necrosis of the acetabular bone fragment after rotational acetabular osteotomy (RAO). The arterial supply of the acetabulum was determined using a corrosion casting method. The acetabular branch of the obturator artery supplies the acetabulum through the acetabular notch. The pubic branches supply the pelvic surface of the acetabulum. Deep branches of the superior gluteal artery supply the superior region and the inferior gluteal artery supplies the postero-inferior region. From these results, we recognized that the important artery for achieving successful RAO is the acetabular branch of the obturator artery that supplies the acetabulum through the acetabular notch.
    Clinical Anatomy 02/1997; 10(2):77-81. · 1.16 Impact Factor
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    ABSTRACT: Several previous studies have indicated that ingestion of ethanol can induce hypocalcemia or osteoporosis. However, few data are available concerning the effects of ethanol on the parathyroid gland. To clarify the mechanism of ethanol-induced hypocalcemia, we studied the ultrastructure to the parathyroid gland in golden hamsters after shortterm treatment with ethanol. Ethanol was administered by gavage via an intragastric tube at 6g/kg of 50% ethanol in distilled water. The mean serum calcium concentration was significantly low at 3 and 5h after administration. The Golgi complexes of the parathyroid chief cells significantly decreased 1 and 3h after administration. The lipid droplets and the large vacuolar bodies significantly increased 5h after administration. These findings suggest that the cellular activity of the parathyroid gland is suppressed after shortterm treatment with ethanol.
    Medical Electron Microscopy 01/1997; 30(3):148-153.
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    ABSTRACT: The effects of streptozotocin treatment on parathyroid water-clear cells in golden hamsters were investigated. In the cytoplasm of the water-clear cells, lipid droplets were increased as compared to that of the control animals. This finding suggests that treatment of streptozotocin affects functional activity in the parathyroid water-clear cells of the golden hamsters.
    Okajimas Folia Anatomica Japonica 06/1996; 73(1):7-13.
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    ABSTRACT: The ultrastructure of the parathyroid glands in golden hamsters after administration of progesterone was investigated. In the parathyroid glands of the female hamsters after administration of progesterone, the mean serum calcium concentration was significantly higher when compared to that of the control hamsters. In the male experimental hamsters, the percentage area occupied by Golgi complexes and lipid droplets was significantly increased when compared to that of the control hamsters, respectively. In the female hamsters after administration of progesterone, the percentage area occupied by large vacuolar bodies was significantly decreased when compared to that of the control hamsters. In the male and female experimental hamsters, the mean number of secretory granules per 100 microns2 of cytoplasm showed a significant increase compared with that of the male and female control hamsters, respectively. These findings suggest that the secretory activity of the parathyroid glands may be stimulated in response to hypocalcemia induced by progesterone.
    Histology and histopathology 11/1995; 10(4):907-11. · 2.28 Impact Factor
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    ABSTRACT: The effects of different ages on large vacuolar bodies in the parathyroid glands of golden hamsters after administration of prostaglandin E2 (PGE2) were investigated. In the parathyroid glands of the young and senile animals 15 min and the senile animals 60 min after administration of PGE2, the mean serum calcium concentration was significantly higher when compared to that of the young and senile control animals, respectively. In the experimental adult animals 60 min after administration of PGE2, the serum calcium concentration was seen to increase. In the parathyroid glands of the young animals 15 min and the adult and senile animals 60 min after administration of PGE2, the percent area occupied by large vacuolar bodies was significantly increased when compared to that of the young, adult and senile control animals, respectively. These findings suggest that the percent area occupied by large vacuolar bodies is increased in response to hypercalcemia induced by PGE2. It is thought that in the parathyroid glands suppressed by hypercalcemia there is a relationship between the percent area occupied by large vacuolar bodies and aging.
    Histology and histopathology 05/1994; 9(2):269-73. · 2.28 Impact Factor
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    ABSTRACT: The ultrastructure of the parathyroid glands of magnesium-treated golden hamsters exposed to a 5 gravity environment was studied. In the parathyroid glands of the magnesium-treated animals exposed to a hypergravity environment, the Golgi complexes and cisternae of the granular endoplasmic reticulum were increased as compared to those of the magnesium-treated animals and decreased as compared to those of animals exposed to a hypergravity environment, but were almost the same as those of the control animals. In the control and experimental animals, the chief cells were rich in free ribosomes and mitochondria. In addition, numerous secretory granules were situated close to the plasma membrane in the magnesium-treated animals exposed to a hypergravity environment. These observations suggest that the synthesis of parathyroid hormone may be stimulated in the parathyroid glands of magnesium-treated hamsters exposed to hypergravity environment.
    Okajimas Folia Anatomica Japonica 01/1994; 70(5):209-17.
  • S Shoumura, S Emura, H Isono
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    ABSTRACT: Since Sandström reported the first detailed description of the parathyroid glands of human beings in 1880, and Lever first described the ultrastructure of the parathyroid chief cells of rat in 1957, a large number of light and electron microscopic studies have been done on the parathyroid glands of numerous animal species under normal and experimental conditions. This review deals with the comparative morphology of the parathyroid glands in amphibians, reptiles, birds and mammals under normal conditions, and the problem concerning the effects of various experimental conditions on the parathyroid glands in mice, rabbits and hamsters. The parathyroid glands were recognized in all vertebrate animals higher than fish, and arose from the third and fourth branchial pouches. Several animals, such as the newt, lizard, gecko, mouse, rat, hamster and gerbil, had only two parathyroid glands, but most animals had four. In mammals, most of the parathyroid glands were closely associated with the thyroid gland, but in amphibians, reptiles and birds, the glands separated from the thyroid gland. In some mammals, the parenchymal cells of the parathyroid gland were classified under a light microscope into two main types of cells: chief cells and oxyphil cells. Examinations under an electron microscope also showed the chief cells having many cell organelles and the oxyphil cells filled with numerous mitochondria in the parathyroid glands of human beings, monkeys, cows, horses, bats and turtles. In addition, the chief cells in most animals were classified at the light microscopic level into light cells and dark cells, moreover the chief cells were also electron microscopically divided into a light and dark type showing different functional phases of a single cell type when osmium or glutaraldehyde fixative was used. However, it is widely accepted today that differences in cytoplasmic density of the chief cells are due to artifacts produced in the process of tissue preparation. The parenchymal cells of the parathyroid gland of the newt were divided into the basal cells (supporting cells) and the suprabasal cells (chief cells). In the parathyroid gland of the frog and toad, blood vessels and connective tissues were not present. In the parathyroid gland of the rabbit and hamster, the water-clear cell was observed. In the electron microscopic radioautograph of the parathyroid gland treated with 3H-leucine, most of the silver grains were seen over cisternae of the granular endoplasmic reticulum at 15 minutes, over the Golgi complexes at 30 minutes, and over secretory granules at 60 minutes.(ABSTRACT TRUNCATED AT 400 WORDS)
    Kaibogaku Zasshi 03/1993; 68(1):5-29.
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    ABSTRACT: We examined the relationship between the incidence of ultrastructural changes in the alveolar septum and the extravascular lung water content. Pulmonary edema was induced in 18 mongrel dogs by either dextran (n = 12) or alloxan (n = 6) administration. Six other dogs served as controls. Extravascular lung water content was measured by the thermal-dye double indicator dilution method. Specimens of lung tissue were examined with an electron microscope, and the incidence of 13 types of pathological changes in the alveolar septum was studied. For each type of pathological change, the incidence was correlated with the magnitude of lung water content. The following results were obtained. The incidence of edematous changes in the alveolar interstitium (widening of the interstitial space, and dispersion and disarray of collagen fibres in the interstitial space) was well correlated with lung water content (r = 0.78, p < 0.01, and r = 0.84, p < 0.01, respectively). The correlation was not significant in the remaining types of changes. We conclude that the incidence of the pathological changes in the alveolar septum is increased along with the increase in the content of lung water in both dextran- and alloxan-induced experimental pulmonary edema in dogs.
    Histology and histopathology 01/1993; 8(1):73-82. · 2.28 Impact Factor
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    ABSTRACT: Isolated parathyroid glands from adult female golden hamsters were incubated on a black Millipore filter in an incubation vessel containing Ham's F-12 medium, with or without melatonin at final concentration of 10(-5) M for 1 hour. In the parathyroid glands used for in vitro treatments with melatonin, the Golgi complexes associated with a few prosecretory granules and cisternae of the rough endoplasmic reticulum showed a significant decrease, and lipid droplets and lysosomes appeared to be increased compared with those of the control parathyroid glands. These changes are considered to be induced by suppression of the synthesis of parathyroid hormone in parathyroid glands incubated in a vessel containing medium with melatonin.
    Histology and histopathology 11/1992; 7(4):715-8. · 2.28 Impact Factor

Publication Stats

167 Citations
52.50 Total Impact Points

Institutions

  • 1994–2007
    • Gifu University
      • Department of Anatomy
      Gihu, Gifu, Japan
  • 1992–2005
    • Gifu University Hospital
      Gihu, Gifu, Japan
  • 1997
    • Aichi Gakuin University
      • Department of Anatomy
      Nagoya-shi, Aichi-ken, Japan