S G Potapov

Publications (17)4.97 Total impact

• Article: The microsatellite polymorphism and gene flow in the contact zone of four common shrew (Sorex araneus L., Mammalia) chromosome races

  O. O. Grigoryeva, A. G. Shestak, S. G. Potapov, Yu. M. Borisov, S. Yu. Irkhin, N. P. Korablev, V. N. Orlov
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  ABSTRACT: The variation of microsatellite loci in 130 individuals of four common shrew chromosome races (Moscow, Western Dvina, Seliger, and St. Petersburg) contacting on the Valdai Hills was studied. A low level of genetic differences between the chromosome races, which differ at three-five fixed diagnostic metacentric chromosomes, was found. The genetic differentiation within the races is more considerable as compared with that between the races. A high deficiency in heterozygotes was recorded; presumably, this is connected with regular variation in the population sizes. It is assumed that the fixation of centric chromosome fusions was supported by selection (drive) in the evolution of the common shrew against the background of a neutral evolution of the microsatellite loci.
  Biology Bulletin 05/2012; 38(5):425-433. · 0.20 Impact Factor
• Article: Evaluation of phylogenetic relationships in the genus Mus using t-specific microsatellite DNA markers

  L. D. Safronova, S. G. Potapov, V. G. Petrosyan, A. I. Chekunova
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  ABSTRACT: The t-complex includes a complex system of genes localized in the proximal region of chromosome 17 of house mouse Mus musculus. The results of microsatellite analysis of laboratory stocks of house mice carrying t 12, t w5, t w12, and t w73 haplotypes and wild mice from natural populations of Russia (Volgograd, Rostov, Saratov oblasts, and Kalmykia), Armenia, Bulgaria, Iran, and Mongolia performed by the PCR method with the use of eight pairs of D17Mit primers (16, 21, 23, 28, 32, 57, 63, 78) are presented. These pairs of primers amplify microsatellite DNA sequences on mouse chromosome 17 in the region from 7.6 to 18.8 cM that correspond to inversions (In (17) 3.4). Each pair of primers recognized three to six variants of nucleotide sequences ranging in size from 90–120 bp (D17Mit 16) to 300–330 bp (D17Mit 57). In most cases, two variants of nucleotide sequences were detected in each individual, i. e., most individuals were heterozygous for the microsatellite loci under study. The highest similarity of the spectra of microsatellite DNA fragments was revealed in laboratory stocks of house mice carrying the t w5 and t w73 haplotypes. The spectra of animals from the Rostov and Volgograd oblasts appeard to be most similar to them. The microsatellite spectra of individuals from Iran closely resemble the spectrum of an individual from Armenia. It was demonstrated that amplified microsatellite fragments localized in the region of the t-complex can be used to identify representatives of the Mus genus from wild populations.
  Russian Journal of Genetics 04/2012; 45(5):580-586. · 0.43 Impact Factor
• Article: Contact zones between chromosome races of the common shrew Sorex araneus L. (insectivora, mammalia) at the margins of the Veps stage of the Valdai ice sheet

  Yu. M. Borisov, A. I. Kozlovsky, A. E. Balakirev, T. B. Demidova, S. Yu. Irchin, V. M. Malygin, N. M. Okulova, S. G. Potapov, A. N. Shchipanov, V. N. Orlov
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  ABSTRACT: Cytogenetic analysis of the common shrew populations in the Valdai Hills revealed the presence of four distinct chromosome races (Western Dvina, Seliger, St. Petersburg, and Moscow). The geographic distribution of these races relative to the borders of the Late Valdai glaciation area is described and discussed. Five contact zones between chromosomal races were found, located directly on the ice-marginal formations of the Veps stage. Two races, Seliger and St. Petersburg, are located exclusively in the former ice sheet area. Attention is drawn to the fact that many of the races located in the last glacial area from the Valdai Hills through Scandinavia are characterized by endemic chromosomes (gi, gp, mo, or, etc.). It is concluded that the situation in the former glaciation area could favor the preservation of tundra or forest vegetation refuges and populations of some small mammal species.
  Contemporary Problems of Ecology 04/2012; 1(5):583-589. · 0.10 Impact Factor
• Article: Genomic versus chromosomal polytypy in studies of mitochondrial and nuclear DNA markers in the Microtus arvalis group

  N. S. Bulatova, S. G. Potapov, L. A. Lavrenchenko
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  ABSTRACT: Common voles of the Microtus arvalis group distributed over the territory of European Russia are represented by three karyotypic categories, i.e., sympatric sibling species with 2n = 46 and 54, and two allopatric karyoforms in one of them, 2n = 46. For each category, molecular markers were found. For two 46-chromosome forms (arvalis and obscurus), iDNA was for the first time studied in karyotyped and non-karyotyped specimens for a parapatric hybrid zone, where high diversity of intermediate karyotypes was recorded. Preferential migration of the mitochondrial markers in arvalis and significant differences in the cline width for chromosomal and nuclear markers in obscurus were shown. The hybrid zone examined exhibited unusual combination of such features as the practically complete absence of “pure” representatives of the original parental forms and a clear deficiency of the first generation hybrids. The mtDNA divergence for the arvalis and obscurus karyoforms (4.6%) is comparable to the lowest limit for interspecies differences within the large and complex genus Microtus.
  Russian Journal of Genetics 04/2012; 46(5):586-594. · 0.43 Impact Factor
• Article: [Microsatellite analysis of two captive populations of sable (Martes zibellina L.)].

  S N Kashtanov, K I Afanas'ev, S G Potapov, O E Lazebnyĭ
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  ABSTRACT: The high value of sable (Martes zibellina L.) fur and stable demand for it over the centuries have led to suboptimal hunting patterns and, as a result, considerable fluctuations in the sizes of natural populations of this species. To maintain the traditional export of sable fur, efforts towards commercial domestication of sable have been made in Russia. The first farm population of sable consisted of animal from eight natural populations in 1929. After the problems related to breeding in captivity were solved, directional selection began. Eighty years of breeding have resulted in sable herds with homogeneous quantitative characters. Prospects for further breeding depend on the current level of genetic diversity in the captive populations of sables formed during the first stages of domestication. The sable populations of the Pushkinsky and Saltykovsky fur farms located in Moscow oblast, which were the objects of this study, are the progenitors of the existing captive populations. The first estimation of genetic variation of this species by means of a panel of microsatellite markers was developed for this study. Two captive sable populations were analyzed using ten microsatellite loci; a total of 75 alleles were found in both populations. Population-specific alleles were identified (6 and 13 in the Pushkinsky and Saltykovsky populations, respectively). The populations studied were found to be differentiated with respect to four microsatellite loci.
  Genetika 12/2011; 47(12):1622-8. · 0.44 Impact Factor
• Article: [A RAPD fingerprinting of sibling species of the Drosophila virilis group].

  S S Mikhaĭlovskiĭ, A M Kulikov, S G Potapov, O E Lazebnyĭ, V G Mitrofanov
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  ABSTRACT: A comparative analysis of the sibling species of Drosophila virilis was performed by RAPD-PCR technique using a set of random primers. The degree of relatedness was studied by cluster analysis (UPGMA) and multi-dimensional scaling. The resulting pattern of species relationships contradicts the classical taxonomy. The main result of the cluster analysis is that D. virilis does not cluster with the remaining three species of its phylad, while according to multidimensional scaling, D. virilis is equidistant from all the species of its group, from both the species of its phylad and the species of the montana phylad. The montana phylad is extremely heterogeneous; moreover, the species D. littoralis, D. ezoana, and D. kanekoi appear to be closer to the virilis phylad than to the other species of the montana phylad, wherein these species are traditionally included. The phylogenetic relationships between the studied species discovered using RAPD fingerprinting comply with the results obtained using protein markers and quantitative traits.
  Genetika 02/2007; 43(1):105-9. · 0.44 Impact Factor
• Article: A RAPD fingerprinting of sibling species of the Drosophila virilis group

  S. S. Mikhailovsky, A. M. Kulikov, S. G. Potapov, O. E. Lazebny, V. G. Mitrofanov
  [show abstract] [hide abstract]
  ABSTRACT: A comparative analysis of the sibling species of Drosophila virilis was performed by RAPD-PCR technique using a set of random primers. The degree of relatedness was studied by cluster analysis (UPGMA) and multidimensional scaling. The resulting pattern of species relationships contradicts the classical taxonomy. The main result of the cluster analysis is that D. virilis does not cluster with the remaining three species of its phylad, while according to multidimensional scaling, D. virilis is equidistant from all the species of its group, from both the species of its phylad and the species of the montana phylad. The montana phylad is extremely heterogeneous; moreover, the species D. littoralis, D. ezoana, and D. kanekoi appear to be closer to the virilis phylad than to the other species of the montana phylad, wherein these species are traditionally included. The phylogenetic relationships between the studied species discovered using RAPD fingerprinting comply with the results obtained using protein markers and quantitative traits.
  Russian Journal of Genetics 12/2006; 43(1):88-91. · 0.43 Impact Factor
• Article: [T-specific D17Leh66 DNA elements in family Muridae].

  S G Potapov, A I Chekunova, I V Shustrova, L A Lavrechenko, L D Safronova
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  ABSTRACT: Blot-hybridization analysis with the use of the t-specific probe D17Leh66 has been used to study DNA of various representatives of family Muridae. Hamsters from genus Phodopus have no homologs of this probe, whereas African rats from genus Lophuromys have some homologous elements. This indicates that sequence Dl7Leh66 is ancient and was probably present in the common ancestor of family Muridae.
  Genetika 01/2006; 41(12):1714-7. · 0.44 Impact Factor
• Article: t-Specific D17Leh66 DNA Elements in the Family Muridae

  S. G. Potapov, A. I. Chekunova, I. V. Shustrova, L. A. Lavrenchenko, L. D. Safronova
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  ABSTRACT: Blot-hybridization analysis with the use of the t-specific probe D17Leh66 has been used to study DNA of various representatives of family Muridae. Hamsters from genus Phodopus have no homologs of this probe, whereas African rats from genus Lophuromys have some homologous elements. This indicates that sequence D17Leh66 is ancient and was probably present in the common ancestor of family Muridae.
  Russian Journal of Genetics 01/2005; 41(12):1425-1427. · 0.43 Impact Factor
• Article: [Genetic differentiation of voles of the tribe Arvicolini (Cricetidae, Rodentia) using taxonprint analysis and polymerase chain reaction with random primers].

  S G Potapov, V N Orlov, Iu M Koval'skaia, V M Malygin, A P Ryskov
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  ABSTRACT: The variability of the genomes of ten vole species was analyzed by means of taxonomic DNA fingerprinting and polymerase chain reaction using random primers (RAPD-PCR). The dendrograms of genetic similarity between the representatives of the tribe Arvicolinae (Gray, 1821) were constructed, based on the data obtained by means of both methods. The topology of the genetic similarity dendrogram that is based on the RAPD-PCR data generally correlates with the genetic distances estimated from biochemical and karyological data. The results did not confirm the genus status of Terricola. On the other hand, the data from taxonprint analysis suggest the recognition of Lasiopodomys as a separate genus.
  Genetika 05/1999; 35(4):484-92. · 0.44 Impact Factor
• Article: [Use of taxonomic typing of DNA for analyzing genomic variability in representatives of Bovidae Gray, 1821].

  S G Potapov, I V Kudriavtsev, A P Ryskov
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  ABSTRACT: Genome variability in representatives of Bison and Bos was studied using taxonomic DNA typing. It was shown that this method, while not revealing individual genomic differences, can be used to differentiate Bovidae representatives at the genus levels. The species-specific DNA fragments found can serve as molecular genetic markers that characterize the genetic variability of the studied animal groups at the taxonomic level.
  Genetika 07/1994; 30(6):858-60. · 0.44 Impact Factor
• Article: [Analysis of the variability of repeated elements in rodent genomes at the taxonomic level].

  S G Potapov, A P Ryskov
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  ABSTRACT: "Taxonomic DNA fingerprints" [1] have been received for a number of rodent species of Muridae family. Some differences in DNA repeated sequences patterns at the species and a higher taxonomic levels are shown. Revealed specific species DNA fragments can be used as molecular-genetic markers for identification and differentiation of various rodent groups, as well as for solving of particular systematic problems.
  Genetika 06/1993; 29(5):869-72. · 0.44 Impact Factor
• Article: Transfer of mitochondrial genome of the northern redbacked vole (Clethrionomys rutilus) to the bank vole (C. glareolus) in northwestern Europe.

  S G Potapov, N A Illarionova, T A Andreeva, M I Baskevich, N M Okulova, L A Lavrenchenko, V N Orlov
  Doklady Biological Sciences 417:435-8.
• Article: Isolation effect in narrow hybrid zones of Sorex araneus chromosome races.

  O O Grigoryeva, A G Shestak, V B Sycheva, S G Potapov, Yu M Borisov, V N Orlov
  Doklady Biochemistry and Biophysics 436:41-3. · 0.33 Impact Factor
• Article: [The microsatellite polymorphism and gene flow in the contact zone of four common shrew (Sorex araneus L., Mammalia) chromosome races].

  O O Grigor'eva, A G Shestak, S G Potapov, Iu M Borisov, S Iu Irkhin, N P Korablev, V N Orlov
  [show abstract] [hide abstract]
  ABSTRACT: The variation of microsatellite loci in 130 individuals of four common shrew chromosome races (Moscow, Western Dvina, Seliger, and St. Petersburg) contacting on the Valdai Hills was studied. A low level of genetic differences between the chromosome races, which differ at three-five fixed diagnostic metacentric chromosomes, was found. The genetic differentiation within the races is more considerable as compared with that between the races. A high deficiency in heterozygotes was recorded; presumably, this is connected with regular variation in the population sizes. It is assumed that the fixation of centric chromosome fusions was supported by selection (drive) in the evolution of the common shrew against the background of a neutral evolution of the microsatellite loci.
  Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk
• Article: The genetic structure of an isolated population of the common shrew Sorex araneus L. (Mammalia) as determined from microsatellite variation.

  O O Grigoryeva, M L Oparin, S G Potapov, V N Orlov
  Doklady Biological Sciences 446(1):300-1.
• Article: A genetic study of natural hybridization between two forms of the common vole (Microtus arvalis) with the use of molecular and cytogenetic methods.

  L A Lavrenchenko, S G Potapov, N Sh Bulatova, F N Golenishchev
  Doklady Biological Sciences 426:222-4.