Xue-Jun Jiang

Renmin University of China, Peping, Beijing, China

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Publications (20)63.49 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The expression of foreign gene was enhanced and prolonged by sustained releasing a target gene to cells from biodegradable dextran-poly(e-caprolactone)-2-hydroxylethylmethacrylate-poly(N-isopropylacrylamide) (Dex-PCL-HEMA/PNIPAAm) hydrogel in vitro. Moreover, we have demonstrated that injection of the same hydrogel improved post-infarct ventricular remodeling. Therefore, we hypothesized that intramyocardial injection of plasmid containing the short-hairpin RNA (shRNA) of angiotensin converting enzyme (ACE) with the same hydrogel enhances the cardioprotective effects superior to either alone or after rat myocardial infarction (MI). In this study, equal volume of phosphate-buffered solution (PBS), 10 μg ACE-shRNA plasmids, hydrogel containing 10 μg negative control ACE-shRNA plasmids and hydrogel containing 10 μg ACE-shRNA plasmids were shortly injected into the infarct area of rats after MI, respectively. We found that ACE-shRNA plasmid-loaded hydrogel extended the duration of gene expression in vivo. Moreover, it was shown that direct intramyocardial injection of ACE-shRNA plasmid-loaded hydrogel significantly decreased the expression of local ACE expression, inhibited cell apoptosis, reduced infarct size, and improved cardiac function compared with the injection of either alone 30 days after MI in rats. These results suggest that injection of ACE-shRNA plasmid-loaded hydrogel into impaired myocardium obtains more cardioprotective effects than either alone in rat with MI by prolonging the gene silencing of ACE. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
    Journal of Biomedical Materials Research Part A 11/2013; · 2.83 Impact Factor
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    ABSTRACT: Critical limb ischemia is regarded as a potentially lethal disease and the treatment effects of existing therapies are limited. Here, in order to develop a potential approach to improve the therapy effects, we designed a peptide of TAT-PKKKRKV as the vector for VEGF165 plasmid to facilitate in vivo angiogenesis. In in vitro studies, TAT-PKKKRKV with low cytotoxicity exhibited efficient transfection ability either with or without serum. Additionally, application of TAT-PKKKRKV/VEGF165 complexes in hindlimb ischemia rats obviously promoted the expression of VEGF protein, which further enhanced effective angiogenesis. The results indicated that TAT-PKKKRKV is an efficient gene vector with low toxicity both in vitro and in vivo, which has great potential for clinical gene therapy.
    Bioconjugate Chemistry 05/2013; · 4.58 Impact Factor
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    ABSTRACT: A facile and flexible targeting gene-delivery strategy is reported by mimicking the biological ligand-receptor principle based on avidin-biotin interaction. In vitro and in vivo investigations demonstrate that this strategy provides a simple, but universal alternative for potential target gene delivery as well as drug delivery.
    Advanced healthcare materials. 10/2012;
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    ABSTRACT: Na(+)/Ca(2+) exchanger (NCX) plays important roles in cardiac electrical activity and calcium homeostasis. NCX current (I(NCX)) shows transmural gradient across left ventricle in many species. Previous studies demonstrated that NCX expression was increased and transmural gradient of I(NCX) was disrupted in failing heart, but the mechanisms underlying I(NCX) remodeling still remain unknown. In present study, we used patch clamp technique to record I(NCX) from subepicardial (EPI) myocytes and subendocardial (ENDO) myocytes isolated from sham operation (SO) mice and heart failure (HF) mice. Our results showed that I(NCX) was higher in normal EPI cells compared with that in ENDO, whatever for forward mode or reverse mode. In HF group, I(NCX) was significantly up-regulated, but EPI-ENDO difference was disrupted because of a more increase of I(NCX) in ENDO myocytes. In order to explore the molecular mechanism underlying remodeling of I(NCX) in failing heart, we detected the protein expression of NCX1 and Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) by Western blot. We found that CaMKII activity was dramatically enhanced and parallel with the expression of NCX1 in failing heart. Our study demonstrated that transmural gradient of I(NCX) existed in murine left ventricle, and increased activity of CaMKII should account for I(NCX) remodeling in failing heart.
    Molecular Biology Reports 07/2011; 39(4):3847-52. · 2.51 Impact Factor
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    ABSTRACT: Many studies have examined the association between coffee consumption and risk of cardiovascular disease, but the results remain controversial. Caffeine is one of the main biologically active compounds of coffee. The aim of this study was to investigate the potential role of caffeine on myocardial ischemia/reperfusion (I/R) injury in the rats. We administered caffeine (25 mg/kg per day) or saline in rats for 4 weeks before myocardial ischemia/reperfusion operation. Compared with the sham group, caffeine treatment decreased ischemia-associated infarct size, serum creatine kinase, and lactate dehydrogenase 3-h reperfusion after 30-min ischemia. Myocardial neutrophil infiltration (assessed by myeloperoxidase activity) was significantly decreased compared with the control group. Meanwhile, caffeine reduced the myocardial apoptosis and suppressed the activation of caspase 3 during myocardial I/R. Importantly, we observed a strong poly(ADP-ribose) polymerase (PARP) activation during myocardial I/R, and caffeine administration inhibited PARP activation and attenuated the expression of PARP-related proinflammatory mediators such as inducible nitric oxide synthetase, IL-6, and TNF-α, all of which may be correlated with downregulated nuclear factor κB activity. We concluded that caffeine protected against myocardial I/R injury by inhibiting inflammation and apoptosis.
    Shock (Augusta, Ga.) 05/2011; 36(3):289-94. · 2.87 Impact Factor
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    ABSTRACT: L-type calcium current (ICa) plays a critical role in excitation–contraction coupling (ECC). Unlike transient outward K+ current (Ito), it is controversial whether ICa transmural gradient exists in left ventricle. Although previous studies have shown some evidences for ICa heterogeneity, the mechanism is still unknown. In this study, the authors recorded ICa from epicardial (EPI) and endocardial (ENDO) myocytes isolated from murine left ventricle using patch-clamp technique. It was found that ICa density was obviously larger in EPI than in ENDO (7.3 ± 0.3 pA/pF vs. 6.2 ± 0.2 pA/pF, at test potential of +10 mV, P Ca showed no difference between these two regions except for the fast inactivation time constants (9.9 ± 0.9 ms in EPI vs. 13.5 ± 0.9 ms in ENDO, at test potential of +10 mV, P Ca transmural gradient by Western blot. The authors demonstrated that a higher activity of CaMKII in ENDO cells induced more nuclear translocation of p65, a component of nuclear factor-kappa B (NF-kB). Consequently, p65 in ENDO inhibited more transcription of Cav1.2, the main encoding gene for L-type calcium channels (LTCCs). These results reveal a difference in CaMKII/p65 signal pathway between EPI and ENDO that underlies this mechanism of ICa heterogeneity in murine left ventricle.
    Molecular and Cellular Biochemistry 01/2011; 352(1):239-246. · 2.33 Impact Factor
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    ABSTRACT: Experimental and clinical studies have suggested that cell implantation could improve cardiac function after myocardial infarction (MI). However, this technique was limited by decreased engraftment and survival of transplanted cells within the ischemic tissue. The present study was performed to investigate whether implantation of bone marrow-derived mononuclear cells (BMMNCs) encapsulated in hydrogel could increase cell engraftment and help to restore cardiac function of MI rabbits. MI was induced in rabbits by coronary artery ligation. One week later, cell culture medium, Dex-PCL-HEMA/PNIPAAm hydrogel, BMMNCs in medium or BMMNCs in hydrogel were injected into the infarcted area of the left ventricle (LV). Increased cell engraftment was observed 48 h after injection when cells were encapsulated in hydrogel; 30 days after treatment, echocardiographic studies showed that injection of BMMNCs in hydrogel preserved LV ejection fraction and attenuated LV dilatation compared with other groups. Histological analysis indicated that injection of BMMNCs in hydrogel enhanced neovascular formation and prevented scar expansion compared with the other groups. Injection of hydrogel-encapsulated BMMNCs increased cell engraftment and improved LV function; this technique may serve as an effective approach to restore infarcted myocardium.
    Cardiology 02/2010; 115(3):194-9. · 1.52 Impact Factor
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    ABSTRACT: To compare the cardioprotective effect of self-assembling peptide (RAD16-II) and thermosensitive hydrogel DPHP (Dex-PCL-HEMA/PNIPAAm) and investigate the optimal property of hydrogel for the treatment of myocardial infarction (MI). MI model was induced by left anterior descending (LAD) coronary artery ligation in SD rats. The animals were randomized into three groups to receive RAD16-II hydrogel (n = 15), DPHP hydrogel (n = 15) or PBS (phosphate buffered saline; control group n = 15); sham-operated rats (n = 10), a suture was tied loosely around left coronary artery without ligating it. At Day 20 post-MI, left ventricle function was evaluated by echocardiography and cardiac catheter. Masson's trichrome was used for histological examination; anti-alpha-smooth muscle antigen (alpha-SMA) was applied to label the neovasculature formation in infarct area. The rats receiving DPHP hydrogel showed a significantly smaller left ventricle end diastolic diameter (LVEDd) and higher levels of left ventricle fraction shortening (LVFS) and left ventricle end systolic pressure (LVESP) than the rats in RAD16-II group [LVEDd (7.9 +/- 0.9) mm vs (8.9 +/- 0.8) mm]; [LVFS (25.4 +/- 5.1)% vs (21.9 +/- 2.9)%; LVESP (114.0 +/- 7.6) mm Hg vs (99.1 +/- 9.6) mm Hg; P < 0.05]. Histological examination showed uncompleted degraded hydrogel in infarct area of DPHP group but not in RAD16-II group. The animals receiving DPHP hydrogel demonstrated significantly a smaller infarct size, a higher infarct wall thickness and a lower vessel density in infarct than the animals receiving RAD16-II hydrogel (P < 0.05). DPHP hydrogel implantation further inhibits the post-MI ventricle remodeling than RAD16-II hydrogel implantation. The mechanism is not correlated with the vascular density in infarct area.
    Zhonghua yi xue za zhi 01/2010; 90(4):259-63.
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    ABSTRACT: Erythropoietin (EPO) can protect myocardium from ischemic injury, but it also plays an important role in promoting polycythaemia, the potential for thrombo-embolic complications. Local sustained delivery of bioactive agents directly to impaired tissues using biomaterials is an approach to limit systemic toxicity and improve the efficacy of therapies. The present study was performed to investigate whether local intramyocardial injection of EPO with hydrogel could enhance cardioprotective effect without causing polycythaemia after myocardial infarction (MI). To test the hypothesis, phosphate buffered solution (PBS), alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel, recombined human erythropoietin (rhEPO) in PBS, or rhEPO in hydrogel were injected into the infarcted area immediately after MI in rats. The hydrogel allowed a sustained release of EPO, which inhibited cell apoptosis and increased neovasculature formation, and subsequently reduced infarct size and improved cardiac function compared with other groups. Notably, there was no evidence of polycythaemia from this therapy, with no differences in erythrocyte count and hematocrit compared with the animals received PBS or hydrogel blank injection. In conclusion, intramyocardial delivery of rhEPO with alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel may lead to cardiac performance improvement after MI without apparent adverse effect.
    Biomaterials 07/2009; 30(25):4161-7. · 8.31 Impact Factor
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    ABSTRACT: Cellular transplantation represents a promising therapy for myocardial infarction (MI). However, it is limited by low transplanted cell retention and survival within the ischemic tissue. This study was designed to investigate whether injectable alpha-cyclodextrin/poly(ethylene glycol)-b-polycaprolactone-(dodecanedioic acid)-polycaprolactone-poly(ethylene glycol) (MPEG-PCL-MPEG) hydrogel could improve cell transplant retention and survival, reduce infarct expansion and inhibit left ventricle (LV) remodeling. Bone marrow-derived stem cells (BMSCs) were encapsulated in alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel and maintained their morphologies during the cell culturing. MTT assays were used for in vitro cell viability studies of the hydrogel and were shown to be non-cytotoxic. Seven days after MI, 100 microl of alpha-cyclodextrin solution containing 2 x 10(7) BMSCs and 100mul of MPEG-PCL-MPEG solution were injected into the infarcted myocardium simultaneously and the solutions solidified immediately. Injection of culture medium or cell alone served as controls. Four weeks after treatment, histological analysis indicated that the hydrogel was absorbed, and the injection of BMSCs with hydrogel had increased cell retention and vessel density around the infarct, and subsequently prevented scar expansion compared with BMSCs injection alone. Echocardiography studies showed that injection of BMSCs with hydrogel increased the LV ejection function and attenuated left ventricular dilatation. This study indicated that the injection of BMSCs with alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel was an effective strategy which could enhance the effect of cellular transplantation therapy for myocardial infarction.
    Acta biomaterialia 06/2009; 5(8):2939-44. · 5.68 Impact Factor
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    ABSTRACT: To observe whether injectable hydrogel implantation could prevent adverse cardiac remodeling and preserve cardiac function in rabbits with myocardial infarction (MI). A novel injectable hydrogel, the copolymer MPM/alpha-CD, which self-assembled between alpha-cyclodextrin and methoxy polyethylene glycol-poly (caprolactone)-(dodecanedioic acid)-poly (caprolactone)-methoxypolyethylene glycol triblock polymer, was synthesized by chemical crosslinking and characterized by biocompatibility and biodegradability. Experimental MI was induced in male rabbits by coronary artery ligation. The MI rabbits were randomly divided into hydrogel group (200 microl MPM/alpha-CD were injected into the infarcted myocardium 7 days after MI) and control group (equal volume phosphate buffered saline myocardial injection, n = 8 each). Four weeks after MPM/alpha-CD implantation, echocardiography, histochemistry and immunohistochemistry were performed. Left ventricle ejection fraction was significantly improved in the hydrogel-treated group (56.1% +/- 8.4%) than that in the control group (37.3% +/- 6.4%, P < 0.05). Histological analysis indicated that hydrogel degraded 4 weeks after hydrogel injection, and prevented scar expansion and wall thinning [(3.08 +/- 0.32) mm vs. (2.18 +/- 0.46) mm in control group, P < 0.05]. Neovasculature formation was similar between the hydrogel group [(100.8 +/- 2.4)/mm(2)] and the control [(98.5 +/- 2.9)/mm(2), P > 0.05]. MPM/alpha-CD could serve as an excellent injectable biomaterial for improves cardiac function and attenuating scar expansion and left ventricular dilation in MI rabbits.
    Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 02/2009; 37(2):174-7.
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    ABSTRACT: Myocardial infarction (MI) remains the commonest cause of cardiac-related death throughout the world. Adverse cardiac remodelling and progressive heart failure after MI are associated with excessive and continuous damage of the extracellular matrix (ECM). In this study, we hypothesized that implantation of hydrogel into infarcted myocardium could replace the damaged ECM, thicken the infarcted wall, and inhibit cardiac remodelling. MI was induced in rabbits by coronary artery ligation; 4 days later, 200 microL Dex-PCL-HEMA/PNIPAAm gel solution was injected into the infarcted myocardium. Injection of phosphate-buffered saline served as control. Thirty days after treatment, histological analysis indicated that injection of the biomaterial prevented scar expansion and wall thinning compared with controls. Echocardiography studies showed that injection of hydrogel increased left ventricular ejection fraction and attenuated left ventricular systolic and diastolic dilatation. Haemodynamic analysis demonstrated improved cardiac function following implantation of the hydrogel. These results suggest that injection of thermosensitive Dex-PCL-HEMA/PNIPAAm hydrogel is an effective strategy that prevents adverse cardiac remodelling and dysfunction in MI rabbits.
    European Journal of Heart Failure 02/2009; 11(1):14-9. · 5.25 Impact Factor
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    ABSTRACT: A series of hydrogels containing a biodegradable dextran (Dex) chain grafted with a hydrophobic poly(-caprolactone)-2-hydroxylethyl methacrylate (PCL-HEMA) chain and a thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) chain were synthesized. The molecular weight of PCL-HEMA was determined by gel permeation chromatography, and the inner morphology of the hydrogel was observed by scanning electron microscopy. The release profiles from the hydrogels were investigated using bovine serum albumen as a model drug. It was found that the release behavior could be adjusted by varying the composition of the hydrogel. In vitro cytotoxicity studies of the hydrogels showed that the copolymer Dex-PCL-HEMA/PNIPAAm exhibited low cytotoxicity. The in vivo degradation and histological studies demonstrated that the hydrogels had good biocompatibility and were promising for use as an injectable polymeric scaffold for tissue engineering applications.
    ACS Applied Materials & Interfaces 02/2009; 1(2):319-27. · 5.90 Impact Factor
  • Xiao-Yan Li, Tao Wang, Xue-Jun Jiang, Tao Lin, Shan Ren
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    ABSTRACT: The differences between cell behaviors in 2-dimension culture in vitro and 3-dimension (3-D) microenvironment in vivo have constituted a major obstacle for tissue engineers. In our research, we study the 3-D culture of ECV304 cells in thermosensitive hydrogel. Human umbilical vein endothelial cells were encapsulated in hydrogel, and then incubated. To determine whether endothelial cells were capable of surviving in Dex-PCL-HEMA/PNIPAAm gels, acridine orange/ethidium bromide (AO/EB) double staining was used to label live and dead cells after two days in culture. 100 muL supernatant was aspirated from each well after 48 hours for the measurement of NO concentration. Cells in gels exhibited mostly rounded morphology or spindlelike shape. After 2 days, gels were degradated as about 70% as the initial, adhered cells were found at the bottom of petri dishes, which escaped from the gels after their degradation. There was little cell apoptosis in 3-D culture. Endothelial cells had greatly proliferated and were at a high density within the matrix after culture for 2 days in growth media. No statistical significance of NO secretion were found between 2-D and 3-D culture.
    01/2009;
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    ABSTRACT: Galactosylated and fluorescein isothiocyanate (FITC) labeled polycaprolactone-g-dextran (Gal-PCL-g-Dex-FITC) polymers were synthesized. The grafted polymers can self-assemble into stable micelles in aqueous medium and in serum. Transmission electron microscopy (TEM) images showed that the self-assembled micelles were regularly spherical in shape. Micelle size determined by size analysis was around 120 nm. The anti-inflammation drug prednisone acetate as a model drug was loaded in the polymeric micelles, and the in vitro drug release was investigated. The galactosylated micelles could be selectively recognized by HepG2 cells and subsequently accumulate in HepG2 cells. The in vivo study demonstrated the relative uptake of the micelles by liver is much higher than the other tissues, indicating that the galactosylated micelles have great potential as a liver targeting drug carrier.
    Biomaterials 01/2009; 30(7):1363-71. · 8.31 Impact Factor
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    ABSTRACT: Supramolecular hydrogels self-assembled by alpha-cyclodextrin and methoxypolyethylene glycol-poly(caprolactone)-(dodecanedioic acid)-poly(caprolactone)-methoxypolyethylene glycol (MPEG-PCL-MPEG) triblock polymers were prepared and characterized in vitro and in vivo. The sustained release of dextran-fluorescein isothiocyanate (FITC) from the hydrogels lasted for more than 1 month, which indicated that the hydrogels were promising for controlled drug delivery. ECV304 cells and marrow mesenchymal stem cells (MSC) were encapsulated and cultured in the hydrogels, during which the morphologies of the cells could be kept. The in vitro cell viability studies and the in vivo histological studies demonstrated that the hydrogels were non-cytotoxic and biocompatible, which indicated that the hydrogels prepared were promising candidates as injectable scaffolds for tissue engineering applications.
    Langmuir 10/2008; 24(18):10306-12. · 4.38 Impact Factor
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    ABSTRACT: Myocardial infarction (MI) and the subsequent heart failure remain one of the leading causes of morbidity and mortality world wide. A number of studies have demonstrated that bioderived materials improve cardiac function after implantation because of their angiogenic potential. In this study, we hypothesized that injection of biomaterials into infarcted myocardium can preserve left ventricular (LV) function through its prevention of paradoxical systolic bulging. To test this hypothesis, infarction was induced in rabbit myocardium by coronary artery ligation. After 1 week, 200-microL alpha-cyclodextrin (alpha-CD)/MPEG-PCL-MPEG hydrogel was injected into the infarcted myocardium. Injection of phosphate buffered saline (PBS) served as controls. Twenty-eight days after the treatment, histological analysis indicated that the injection of hydrogel prevented scar expansion and wall thinning compared with the control (p < 0.05) without more microvessel density in infarcted myocardium (p = 0.70). LV ejection fraction, determined by echocardiography, was significantly greater in the hydrogel-treated group (56.09% +/- 8.42%) than the control group (37.26% +/- 6.36%, p = 0.001). The LV end-diastolic and end-systolic diameters were 2.07 +/- 0.33 cm and 1.74 +/- 0.30 cm, respectively, in the control group. Smaller LV end-diastolic diameter (1.61 +/- 0.26 cm, p = 0.005) and smaller end-systolic diameter (1.17 +/- 0.23 cm, p = 0.001) were found in the hydrogel-treated group. These results suggest that alpha-CD/MPEG-PCL-MPEG hydrogel could serve as an injectable biomaterial that prevents LV remodeling and dilation for the treatment of MI.
    Journal of Biomedical Materials Research Part A 06/2008; 90(2):472-7. · 2.83 Impact Factor
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    ABSTRACT: The hyperpolarization-activated, cyclic nucleotide-gated cation channels (HCN) have been identified as a key factor of cardiac pacemaker activity. The present study investigated the feasibility of using transfection of HCN4, an important subunit in the HCN family, to cure an experimental cardiac bradyarrhythmia. Two adenoviral vectors containing HCN4 and GFP (Ad-HCN4) or GFP alone (Ad-GFP) were constructed. Three or four days after gene injection, the pigs underwent catheter ablation of the atrioventricular (AV) node. After a complete AV block was created, the idioventricular heart rate in the Ad-HCN4 group was significantly greater than in controls. The heart rhythm in the Ad-HCN4 group could be modulated by the beta-adrenergic agonist isoproterenol. An I(f) current could be observed in the ventricular myocytes of the Ad-HCN4 group under patch clamp technique investigations. The expected cell membrane localization of GFP-tagged HCN4 expression was confirmed with confocal fluorescent microscopy. The successful in vivo transfection with Ad-HCN4 was also identified by real-time reverse transcription polymerase chain reaction (RT-PCR). Our study suggested that site-specific gene therapy for cardiac bradyarrhythmias using adenoviral vectors to overexpress HCN4 channels might be feasible.
    Life Sciences 05/2007; 80(19):1746-53. · 2.56 Impact Factor
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    ABSTRACT: To investigate whether autologous transplantation of adult stem cells could improve post-infarcted heart function. Bone marrow mononuclear cells (MNCs) were isolated from adult rabbits' tibias after coronary ligation. These cells were exposed to 5-azacytidine 10 micromol/L for 24 h on the third day of culture. After being labeled with bromodeoxyuridine (BrdU), the cells were auto-transplanted into bordering zone of the infarcted area at 2 weeks after injury. The animals were killed at 3 days, 2 weeks, 1 month, and 2 months after transplantation, respectively. The left ventricular functions, capillary density, and cardiac nerve density were measured and the differentiation of the engrafted cells was determined by immunostaining. BrdU-labeled MNCs were well aligned with the host cardiomyocytes. Parts of them were incorporated into capillary and arteriolar vessel walls. In addition to inducing angiogenic ligands (basic fibroblast growth factor, vascular endothelial growth factor) and inflammation cytokines (interleukin 1-beta) during the early period of MNCs implantation, MNCs induced 2.0-fold increase in capillary density as well. Moreover, GAP43-positive and TH-positive nerve density were markedly higher in the MNCs-treated groups than that in the non-treated hearts. Left ventricular ejection fraction, LV+dp/dt(max), and LV-dp/dt(max) were 47%, 67%, and 55% in MNCs-treated heart respectively, which was higher than that of the control heart, whereas left ventricular end-diastolic volume, left ventricular end-diastolic diameter, and left ventricular end-diastolic pressure were 45%, 22%, and 50% respectively in MNCs-treated heart, which was lower than that of the control heart at 2 months after cell transplantation. Autologous transplantation of MNCs induced angiogenesis and nerve sprouting and improved left ventricular diastolic function.
    Acta Pharmacologica Sinica 08/2004; 25(7):876-86. · 2.35 Impact Factor
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    ABSTRACT: A disulfide bond containing polypeptide, PolyK6-R8, was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK6HC and CR8C. The molar ratio between CHK6HC and CR8C within obtained PolyK6-R8 was 9:1, and PolyK6-R8 could combine DNA compactly when weight ratio reached 5. Through in vitro investigation, it was found that PolyK6-R8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells. Cellular uptake of DNA mediated by PolyK6-R8/DNA complexes was promoted after incubation for 4 h. Moreover, by examining the histological sections of hindlimb ischemia rats through immunohistochemistry, PolyK6-R8/VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation. The results indicated that polypeptide-based PolyK6-R8 is a promising gene vector for the limb ischemia treatment.
    Chinese Journal of Polymer Science 31(5). · 1.27 Impact Factor