Publications (9)23.07 Total impact
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Article: Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L.
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ABSTRACT: An es-t (early senescence-temporary) mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identified in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffin section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromosome 5. These results will facilitate the positional cloning and functional studies of the gene. Keywords Oryza sativa –morphological and physiological analysis–genetic mechanism–map-based cloningChinese Science Bulletin 04/2012; 56(23):2437-2443. · 1.32 Impact Factor -
Article: Identification of a novel tillering dwarf mutant and fine mapping of the TDDL(T) gene in rice (Oryza sativa L.)
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ABSTRACT: Rice plant architecture is an important agronomic trait that affects the grain yield. To understand the molecular mechanism that controls plant architecture, a tillering dwarf mutant with darker-green leaves derived from an indica cultivar IR64 treated with EMS is characterized. The mutant, designated as tddl(t), is nonallelic to the known tillering dwarf mutants. It is controlled by one recessive nuclear gene, TDDL(T), and grouped into the dn-type dwarfism according to Takeda’s definition. The dwarfism of the mutant is independent of gibberellic acid based on the analyses of two GA-mediated processes. The independence of brassinosteroid (BR) and naphthal-3-acetic acid (NAA) of the tddl(t) mutant, together with the decreased size of parenchyma cells in the vascular bundle, indicates that the TDDL(T) gene might participate in another hormone pathway. TDDL(T) is fine mapped within an 85.51 kb region on the long arm of rice chromosome 4, where 20 ORFs are predicted by RiceGAAS (http://ricegaas.dna.affrc. go.jp/rgadb/). Further cloning of TDDL(T) will benefit both marker assisted selection (MAS) of plant architecture and dissection of the molecular mechanism underlying tillering dwarf in rice.Chinese Science Bulletin 04/2012; 54(12):2062-2068. · 1.32 Impact Factor -
Article: Identification and characterization of SHORTENED UPPERMOST INTERNODE 1, a gene negatively regulating uppermost internode elongation in rice.
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ABSTRACT: In rice, the elongated internodes are derived from the vegetative shoot apical meristem (SAM), and the transition of the SAM from the vegetative to the reproductive stage induces internode elongation. In this study, we characterize two shortened uppermost internode mutants (sui1-1 and sui1-2). During the seedling and tillering stages, sui1 plants are morphologically similar to wild-type plants. However, at the heading stage, the sui1-1 mutant exhibits a shortened uppermost internode and a partly sheathed panicle, and the sui1-2 mutant shows an extremely shortened uppermost internode and a fully sheathed panicle. Gibberellin treatment results in elongation of every internode, but the shortened uppermost internode phenotype remains unaltered. Microscopic analysis indicates that cell length of sui1-1 uppermost internode exhibits decreased. Map-based cloning revealed that SUI1 is located on Chromosome 1, and encodes a putative phosphatidyl serine synthase (PSS) family protein. Searches for matches in protein databases showed that OsSUI1 contains the InterPro domain IPR004277, which is conserved in both animal and plant kingdoms. Introduction of a wild-type SUI1 gene fully rescued the mutant phenotype of sui1-1 and sui1-2, confirming the identity of the cloned gene. Consistent with these results, the SUI1-RNAi transgenic plants displayed decreased elongation of the uppermost internode. Our results suggest that SUI1 plays an important role in regulating uppermost internode length by decreasing longitudinal cell length in rice.Plant Molecular Biology 09/2011; 77(4-5):475-87. · 4.15 Impact Factor -
Article: Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.).
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ABSTRACT: In this study, one major QTL affecting gel consistency (GC) of japonica/indica cross was identified on chromosome 6 using a DH population. To understand the molecular mechanism that regulates GC in rice grains, the major QTL (qGC-6) was isolated through a map-based cloning approach utilizing chromosome segment substitution lines (CSSLs). Using 64 plants with extremely soft GC that were selected on recombinant break points between two SSR markers, RM540 and RM8200 in a BC4F2 population, qGC-6 was mapped to a 60-kb DNA region between two STS markers, S26 and S27. These two markers were then used to further identify recombination break points. Finally, qGC-6 was delimited in an interval of a 11-kb region. Gene prediction analysis of the 11-kb DNA sequence containing qGC-6 identified only one putative ORF, which encodes granule-bound starch synthesis protein (Wx protein). Results of sequencing analysis and complementation experiment confirmed that this candidate ORF is responsible for rice GC. Genetic evidences revealed that Wx might contribute equally to the grain amylose content-controlling gene as well as gel consistency. This new information is important to breed rice varieties with improved grain quality.Theoretical and Applied Genetics 06/2011; 123(5):859-67. · 3.30 Impact Factor -
Article: Genetic analysis of leaffolder resistance in rice.
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ABSTRACT: A double haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid 'CJ06'/'TN1', was used to investigate the genetic basis for rice leaffolder resistance. Using a constructed molecular linkage map, five QTLs for rolled leaves were detected on chromosomes 1, 2, 3, 4, and 8. The positive alleles from CJ06 on chromosomes 3, 4, and 8 increased the resistance to rice leaffolder, and the alleles from TN1 on chromosomes 1 and 2 also enhanced resistance to leaffolder. The interactions between QTLs were identified and tested, and four conditional interactions were acquired for resistance to rice leaffolder. These loci were located on chromosomes 2, 9, 10, and 11, respectively. QTL pyramiding indicated that the positive alleles affect resistance to leaffolder. The prospective application of this data in rice breeding was also discussed.Journal of Genetics and Genomics 05/2010; 37(5):325-31. · 1.88 Impact Factor -
Article: Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice.
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ABSTRACT: Leaf morphology is an important agronomic trait in rice breeding. We isolated three allelic mutants of NARROW AND ROLLED LEAF 1 (nrl1) which showed phenotypes of reduced leaf width and semi-rolled leaves and different degrees of dwarfism. Microscopic analysis indicated that the nrl1-1 mutant had fewer longitudinal veins and smaller adaxial bulliform cells compared with the wild-type. The NRL1 gene was mapped to the chromosome 12 and encodes the cellulose synthase-like protein D4 (OsCslD4). Sequence analyses revealed single base substitutions in the three allelic mutants. Genetic complementation and over-expression of the OsCslD4 gene confirmed the identity of NRL1. The gene was expressed in all tested organs of rice at the heading stage and expression level was higher in vigorously growing organs, such as roots, sheaths and panicles than in elsewhere. In the mutant leaves, however, the expression level was lower than that in the wild-type. We conclude that OsCslD4 encoded by NRL1 plays a critical role in leaf morphogenesis and vegetative development in rice.Plant Molecular Biology 02/2010; 73(3):283-92. · 4.15 Impact Factor -
Article: Quantitative trait loci mapping of flag-leaf ligule length in rice and alignment with ZmLG1 gene.
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ABSTRACT: A doubled haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid ‘CJ06’/‘TN1’, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci (QTL) mapping using the DH population. Five QTLs (qLL-2, qLL-4, qLL-6, qLL-10 and qLL-12) controlling the ligule length (LL) were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL-4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.Journal of Integrative Plant Biology 04/2009; 51(4):360-6. · 2.53 Impact Factor -
Article: Genetic analysis and fine mapping of two genes for grain shape and weight in rice.
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ABSTRACT: To identify genetic loci controlling grain weight, an elite indica rice variety, Baodali, with large grains was identified and used in this study. Its derived F(2), F(3) and BC(2)F(2) with another japonica rice variety Zhonghua 11 were used as mapping populations. Linkage analyses demonstrated that two genes controlling grain weight, designated as GW3 and GW6, were mapped to chromosome 3 and chromosome 6, respectively. Fine mapping delimited GW3 to a 122 kb physical distance between two sequence tagged site markers (WGW16 and WGW19) containing 16 open reading frames annotated by The Institute for Genomic Research (http://www.tigr.org). GW6 was further mapped between two simple sequence repeat markers (RM7179 and RM3187). These results are useful for both marker assisted selection of grain weight, and for further cloning of GW genes, which will contribute to the dissection of the molecular mechanism underlying grain weight in rice.Journal of Integrative Plant Biology 02/2009; 51(1):45-51. · 2.53 Impact Factor -
Article: Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice.
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ABSTRACT: A dwarf mutant of rice (Oryza sativa L.) by mutagenesis of ethylene methylsulfonate (EMS) treatment from Nipponbare was identified. The mutant exhibited phenotypes of dwarfism and withered leaf tip (dwl1). Based on the internode length of dwl1, this mutant belongs to the dm type of dwarfing. Analysis of elongation of the second sheath and alpha-amylase activity in endosperm showed that the phenotype caused by dwl1 was insensitive to gibberellin acid treatment. Using a large F2 population derived from a cross between the dwl1 and an indica rice variety, TN1, the DWL1 gene was mapped to the terminal region of the long arm of chromosome 3. Fine-mapping delimited it into a 46 kb physical distance between two STS markers, HL921 and HL944, where 6 open reading frames were predicted. Cloning of DWL1 will contribute to dissecting molecular mechanism that regulates plant height in rice, which will be beneficial to molecular assisted selection of this important trait.Journal of Genetics and Genomics 01/2009; 35(12):715-21. · 1.88 Impact Factor
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Institutions
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2009–2012
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China National Rice Research Institute
Hangzhou, Zhejiang Sheng, China
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