C L Yuan

Publications (12)37.09 Total impact

• Article: Molecular detection of Torque teno sus virus from tissues samples of sick pigs in China.

  C X Zhu, C L Yuan, L Cui, Y Yu, R A Liu, W Zhao, X G Hua
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  ABSTRACT: In the present study, Torque teno sus virus (TTSuV) was detected from different tissues, stool and serum samples of 25 sick pigs. The total prevalence of TTSuV1 and TTSuV2 were 64% (16/25) and 28% (7/25), 24% (6/25) were co-infected with both TTSuV1 and TTSuV2. The prevalence of TTSuV infection in spleen is a slightly higher, with positive rates of 52% (13/25) for TTSuV1 and 24% (6/25) for TTSuV2. Phylogenetic analysis of TTSuV1 showed that 21 isolates were distributed into two clusters (genotype TTSuV1a and TTSuV1b), with genotype TTSuV1b was the dominant genotype. Phylogenetic analysis of TTSuV2 showed that the nine isolates shared 80.9-99.2% nucleotide homology with each other, and were distributed in different genotypes (TTSuV2a-TTSuV2f). TTSuV2d was the most prevalent genotype in this study, which contained five Spanish strains and nine Chinese strains, and shared 94.2-96.8% homology.
  Virus Research 02/2012; 165(2):225-30. · 2.94 Impact Factor
• Article: Segmentation expression of capsid protein as an antigen for the detection of avian nephritis virus infection in chicken flocks.

  W Zhao, A L Zhu, Y Yu, C L Yuan, C X Zhu, D L Lan, Z B Yang, L Cui, X G Hua
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  ABSTRACT: Subclinical pathological changes in the kidneys of broiler chickens and suppression of growth caused by the avian nephritis virus (ANV) affect poultry flocks worldwide. A test for detection of virus-specific antibodies in serum would be useful for epidemiological investigations, however the poor propagation in cell cultures has restricted the development of serological tests based on the use of ANV particles as antigens. An enzyme-linked immunosorbent assay (ELISA) was developed for detection of ANV-specific antibodies in chicken serum, using a recombinant protein antigen prepared by segmentation expression of the capsid protein antigen epitope of ANV (HM029238) transfected into Escherichia coli. The expressed fusion protein was detected by Western blotting with ANV-positive serum, and the optimal immunoreactive fusion P1 protein was determined. Using the optimized P1-ELISA, ANV-specific antibodies were detected in commercial chicken flocks aged 10-25 days obtained from the Liaoning Province, China. Out of 960 serum samples, 459 (47.8%) were positive for infection with ANV. These results indicate that the P1-ELISA is helpful for preliminary serological diagnosis of ANV infection, and could be used to for screening in ANV infection and for determining antibodies against ANV.
  Journal of virological methods 01/2012; 179(1):57-61. · 2.13 Impact Factor
• Article: Detection of astrovirus infection in pigeons (Columbia livia) during an outbreak of diarrhoea.

  W Zhao, A L Zhu, C L Yuan, Y Yu, C X Zhu, D L Lan, Z B Yang, L Cui, X G Hua
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  ABSTRACT: Avian astrovirus infections are widespread in many countries, and infections have been linked to enteritis and increased mortality in young poultry. Although pigeons are treated as an important poultry product in some countries, their diseases are often poorly understood and astrovirus infection in pigeons has not been reported. In the present study, faecal samples were collected during an outbreak of gastrointestinal illness in a population of Shanghai pigeons. The samples were examined for astroviruses by reverse transcription-polymerase chain reaction. Eighty-nine per cent (40/45) and 4% (2/45) were found to be positive for avian nephritis virus (ANV) and chicken astrovirus, respectively. One positive sample indicated a co-infection with both ANV and chicken astrovirus. Phylogenetic analysis based on the partial polymerase gene sequence and full-length capsid protein from published avian astrovirus sequences in GenBank revealed that the pigeon viruses detected in this study were evolutionarily closely related to chicken ANV. The present study provided evidence for the presence of astrovirus in pigeons and suggests that cross-infection between pigeons and commercial chickens was likely. Whether the astroviruses in pigeons were responsible for the diarrhoea remains to be determined.
  Avian Pathology 08/2011; 40(4):361-5. · 1.71 Impact Factor
• Article: Complete sequence and genetic characterization of pigeon avian nephritis virus, a member of the family Astroviridae.

  W Zhao, A L Zhu, Y Yu, C L Yuan, C X Zhu, Z B Yang, L Cui, X G Hua
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  ABSTRACT: In the current study, the complete genome sequence of a member of the family Astroviridae isolated from pigeons was determined through genetic characterization and phylogeny analysis. The isolated genome sequence was proposed to be that of pigeon avian nephritis virus (ANV), whose genome structure and characteristics were similar to previously reported avian astroviruses. The sequenced ssRNA genome comprises 6928 nucleotides, excluding the poly(A) tail, and contains three open reading frames. Phylogenetic analysis using a partial nucleotide sequence of the polymerase gene and the entire amino acid sequence of the full-length capsid protein revealed that pigeon avian nephritis virus is closely related to the previously published ANV, especially to the Japanese G-4260 and Chinese strains. This investigation provides information on the sequence and genetic characteristics of this virus and contributes to a better understanding of pigeon ANV and the possible occurrence of astrovirus transmission between chickens and pigeons.
  Archives of Virology 05/2011; 156(9):1559-65. · 2.11 Impact Factor
• Article: Molecular detection of Saffold Virus in children in Shanghai, China.

  X Q Dai, C L Yuan, Y Yu, W Zhao, Z B Yang, L Cui, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 02/2011; 50(2):186-7. · 3.12 Impact Factor
• Article: Molecular detection and sequence analysis of feline Torque teno virus (TTV) in China.

  C X Zhu, T L Shan, L Cui, X N Luo, Z J Liu, S D Tang, Z W Liu, C L Yuan, D L Lan, W Zhao, X G Hua
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  ABSTRACT: In the present study, two isolates (SH-F1 and SH-F2) of Torque teno felis virus (feline TTV) were detected in 16 (12.5%) serum samples collected from cats in China. Their full length genomes were cloned and sequenced. The results showed that they were 2063bp in length and contained three open reading frames (ORFs) (ORF1: nt438-1748, ORF2: nt268-585 and ORF3: nt268-581, 1461-1842). Phylogenetic analysis showed that they were clustered with the strain of Japan (Fc-TTV4, AB076003) and the strain of France (PRA4, EF538878). Sequence analysis indicated that SH-F1 had high (97.5% and 93.3%) identity with the strain of Japan and the strain of France, and SH-F2 shared 94.5% and 92.1% homology with them, respectively. In conclusion, we demonstrated that feline TTV is present in China.
  Virus Research 12/2010; 156(1-2):13-6. · 2.94 Impact Factor
• Article: Porcine torque teno virus infections in China.

  C X Zhu, L Cui, T L Shan, X N Luo, Z J Liu, C L Yuan, D L Lan, W Zhao, Z W Liu, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 08/2010; 48(4):296-8. · 3.12 Impact Factor
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  Available from: Tongling Shan

  Article: Human parechovirus infections in monkeys with diarrhea, China.

  T L Shan, C M Wang, L Cui, Eric Delwart, C L Yuan, W Zhao, W Guo, X Q Dai, Y Yu, X G Hua
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  ABSTRACT: Information about human parechovirus (HPeV) infection in animals is scant. Using 5' untranslated region reverse transcription-PCR, we detected HPeV in feces of monkeys with diarrhea and sequenced the complete genome of 1 isolate (SH6). Monkeys may serve as reservoirs for zoonotic HPeV transmissions and as models for studies of HPeV pathogenesis.
  Emerging Infectious Diseases 07/2010; 16(7):1168-9. · 6.79 Impact Factor
• Article: The first detection of human parechovirus infections in China.

  T L Shan, W Guo, L Cui, X G Shang, X Q Dai, C L Yuan, Y Yu, W Zhang, J G Zhu, Q Shen, Z B Yang, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 09/2009; 45(4):371-2. · 3.12 Impact Factor
• Article: The first detection of human bocavirus 2 infections in China.

  T L Shan, W Zhang, W Guo, L Cui, C L Yuan, X Q Dai, Q Shen, Z B Yang, J G Zhu, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 09/2009; 46(2):196-7. · 3.12 Impact Factor
• Article: Human astrovirus infection in children with gastroenteritis in Shanghai.

  T L Shan, X Q Dai, W Guo, W Zhang, L Cui, C L Yuan, X G Shang, Q Shen, X G Hua
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 02/2009; 44(3):248-9. · 3.12 Impact Factor
• Article: Cross-species infection of hepatitis E virus in a zoo-like location, including birds.

  W Zhang, Q Shen, J Mou, Z B Yang, C L Yuan, L Cui, J G Zhu, X G Hua, C M Xu, J Hu
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  ABSTRACT: Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animals are considered to be reservoirs. Thirty-eight faecal samples, obtained from 22 species of animals including birds in a wildlife first-aid centre in Eastern China, were tested for HEV RNA. Our survey revealed that in total 28.9% (95% confidence interval 14.5-43.4) of the faecal samples from various mammals and birds were HEV RNA positive. Sequence and phylogenetic analyses of the 11 isolates demonstrated that all sequences clustered in genotype 4 with 96-100% identity to each other. In addition, serum samples from seven animal handlers have shown that five (71.4%) were seropositive. The findings imply that cross-species infection of HEV had probably occurred in this zoo-like location, and moreover, birds can be infected naturally with mammalian HEV.
  Epidemiology and Infection 09/2008; 136(8):1020-6. · 2.84 Impact Factor