Krisztián Bányai

Publications (64)175.11 Total impact

• Article: Review of group A rotavirus strains reported in swine and cattle.

  Hajnalka Papp, Brigitta László, Ferenc Jakab, Balasubramanian Ganesh, Simona De Grazia, Jelle Matthijnssens, Max Ciarlet, Vito Martella, Krisztián Bányai
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  ABSTRACT: Group A rotavirus (RVA) infections cause severe economic losses in intensively reared livestock animals, particularly in herds of swine and cattle. RVA strains are antigenically heterogeneous, and are classified in multiple G and P types defined by the two outer capsid proteins, VP7 and VP4, respectively. This study summarizes published literature on the genetic and antigenic diversity of porcine and bovine RVA strains published over the last 3 decades. The single most prevalent genotype combination among porcine RVA strains was G5P[7], whereas the predominant genotype combination among bovine RVA strains was G6P[5], although spatiotemporal differences in RVA strain distribution were observed. These data provide important baseline data on epidemiologically important RVA strains in swine and cattle and may guide the development of more effective vaccines for veterinary use.
  Veterinary Microbiology 04/2013; · 3.33 Impact Factor
• Article: Detection of novel porcine bocaviruses in fecal samples of asymptomatic pigs in Cameroon.

  Valentine Ngum Ndze, Dániel Cadar, Attila Cságola, Péter Kisfali, Eszter Kovács, Szilvia Farkas, Akum Felix Ngu, Mathew Dioh Esona, Adám Dán, Tamás Tuboly, Krisztián Bányai
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  ABSTRACT: Improvements and widespread use of nucleic acid amplification and sequencing methods have led to the recognition of new virus diversity in various domestic animals, including pigs. In this study we utilized either virus species specific or broadly reactive PCR assays to describe the occurrence and genetic diversity of selected DNA viruses belonging to families Adenoviridae, Circoviridae, Anelloviridae and Parvoviridae in Cameroonian pigs. Fecal specimens were collected during spring of 2011. No adenoviruses, circoviruses and anelloviruses were detected, however, high prevalence and remarkable genetic diversity within the identified parvoviruses and, particularly, within bocaviruses was observed. PPV4 was the most prevalent virus (20%), followed by PBoV3 (18%), PBoV4 (18%), PBoV5 plus 6V/7V (16%), and PBoV1 plus PBoV2 (6%). The frequency of mixed infections with various combinations of these virus species reached 20%. Genetic analysis of the identified viruses showed that the capsid gene of PBoV1 and PBoV2 strains shared up to 91% and 94% nt sequence similarities to reference PBoV1 and PBoV2 strains, respectively. The identified PBoV3 and PBoV4 strains shared ⩽95% and ⩽98% nt identities with reference PBoV3 and PBoV4 strains, respectively, along the NS gene, whereas the PBoV5 strains shared 86% nt identities with Hungarian and 87% nt identities with Chinese PBoV5 strains along the capsid gene. In addition, a single PBoV5-like strain shared ⩽ 71% nt sequence identity with other PBoV5 strains. This is the first study to report evidence of the circulation of bocaviruses in Africa and contributes to our understanding of the impact of globalization on the dispersal of new and emerging viruses.
  Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 03/2013; · 3.22 Impact Factor
• Article: Emergence and characterisation of pandemic H1N1 influenza viruses in Hungarian swine herds.

  Adám Bálint, István Kiss, Krisztián Bányai, Imre Biksi, Katalin Szentpáli-Gavallér, Tibor Magyar, István Jankovics, Mónika Rózsa, Bálint Szalai, Mária Takács, Adám György Tóth, Adám Dán
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  ABSTRACT: In 2010, two novel porcine H1N1 influenza viruses were isolated from pigs with influenza-like illness in Hungarian swine herds. Sequence and phylogenetic analysis of these strains revealed that they shared molecular features with the pandemic H1N1 influenza virus strains, which emerged globally during 2009. The PB2, HA and NA genes contained unique amino acid changes compared to the available new H1N1 influenza virus sequences of pig origin. Furthermore, the investigated strains could be separated with respect to parallel amino acid substitutions affecting the polymerase genes (PB2, PB1 and PA) and the nucleoprotein (NP) gene, supporting the proposed complementarities between these proteins, all required for the viral fitness. Molecular characterisation of two Hungarian human pandemic H1N1 isolates was also performed, so that we could compare contemporaneous strains of different host species origins. Shared molecular motifs in various genes of animal and human influenza strains suggested that the Hungarian porcine strains could have originated from humans through direct interspecies transmission. This study is among the few that support the natural human-to-pig transmission of the pandemic H1N1 influenza virus.
  Acta Veterinaria Hungarica 03/2013; 61(1):125-34. · 0.67 Impact Factor
• Article: Shared G12 VP7 gene among human and bovine rotaviruses detected in cameroonian villages.

  Valentine Ngum Ndze, Eric Akum Achidi, Hajnalka Papp, Eszter Kovács, Szilvia Farkas, Dieudonne Adiogo, Péter Kisfali, Marxcel Bong Ngeng, Marie Therese Obama Abena, Vito Martella, Mathew Dioh Esona, Krisztián Bányai
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  ABSTRACT: Group A rotaviruses (RVA) are an important enteric pathogen in humans and livestock animals. Transmission of animal RVA strains to humans has been documented on several occasions. A reverse route of transmission of RVA under natural circumstances is anticipated, although evidence is scarce. However, experimental studies indicated that animals can be infected with human RVAs. By screening the stool samples collected from 157 cattle during 2011 in two Cameroonian villages, four samples (2.5%) were found positive for RVA. Upon sequence analysis of a 410 bp fragment of the VP7 gene, the RVA strains shared up to 100% nt identity to each other and to G12 RVAs identified in human patients living in the same geographic regions. This finding provides evidence for a human-to-animal transmission of an epidemic human rotavirus strain.
  Acta Microbiologica et Immunologica Hungarica 03/2013; 60(1):21-8. · 0.79 Impact Factor
• Article: Serologic Evidence of Crimean-Congo Hemorrhagic Fever Virus Infection in Hungary.

  Viktória Németh, Miklós Oldal, László Egyed, Miklós Gyuranecz, Károly Erdélyi, Krisztián Kvell, Nikolay Kalvatchev, Herve Zeller, Krisztián Bányai, Ferenc Jakab
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  ABSTRACT: Abstract Crimean-Congo hemorrhagic fever virus (CCHFV) is a typical tick-borne pathogen that causes an increasing number of severe infections in many parts of Africa, Asia, the Middle East, and the Balkans, as well as in some other parts of Europe. The virus is transmitted primarily by Hyalomma spp., and the spectrum of natural hosts for CCHFV is broad, including wild and domestic animals. Although, the presence of CCHFV was hypothesized in Hungary, no significant research activity has been carried out in the past 30 years. In the present study, we provide serological evidence of CCHFV infection in Lepus europeus using newly developed antibody detection assays. Of 198 samples, 12 (6%) were positive for immunoglobulin G antibody against CCHFV, with 2 independent detection assays. This observation indicates a need for a large-scale surveillance to estimate the potential public health risk of CCHFV in Hungary.
  Vector borne and zoonotic diseases (Larchmont, N.Y.) 02/2013; · 2.61 Impact Factor
• Article: Genome Sequence of a Monoreassortant H1N1 Swine Influenza Virus Isolated from a Pig in Hungary.

  Krisztián Bányai, Eszter Kovács, Adám György Tóth, Imre Biksi, Katalin Szentpáli-Gavallér, Adám Bálint, László Dencso, Adám Dán
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  ABSTRACT: The genome of a porcine H1N1 influenza A strain is reported in this study. The strain proved to be a monoreassortant strain with a typical porcine N1 gene on the genetic backbone of the pandemic H1N1 influenza A virus strain. Monitoring of descendants of the pandemic 2009 H1N1 strain is needed because of concerns that more-virulent strains may emerge in forthcoming epidemic seasons.
  Journal of Virology 12/2012; 86(23):13133. · 5.40 Impact Factor
• Article: Novel NSP4 genotype in a camel G10P[15] rotavirus strain.

  Hajnalka Papp, Laila Zaar Al-Mutairi, Wassim Chehadeh, Szilvia L Farkas, György Lengyel, Ferenc Jakab, Vito Martella, György Szűcs, Krisztián Bányai
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  ABSTRACT: In this study a Kuwaiti camel rotavirus strain, RVA/Camel-wt/KUW/s21/2010/G10P[15], is characterized by sequencing and phylogenetic analysis. The strain had multiple genes with high nucleotide sequence similarities to ovine and bovine strains (VP2, ≤ 96%; NSP2 and NSP5, ≤ 97%, NSP3, ≤ 94%), or, to porcine strains (VP1, ≤ 89%). Other genes had moderate sequence similarities (VP4, ≤ 87%; VP6, ≤ 81%; VP7, ≤ 82%) with reference strains from ruminants. The NSP4 gene shared limited sequence identity (≤ 71%) with other mammalian and avian rotavirus NSP4 types, and was designated a novel genotype, E15. This study demonstrates genetic diversity in the outer capsid and some backbone genes of an old-world camelid rotavirus strain and uncovers its common evolutionary roots with strains from other ruminants.
  Acta Microbiologica et Immunologica Hungarica 09/2012; 59(3):411-21. · 0.79 Impact Factor
• Article: Identification of a G8P[14] Rotavirus Isolate Obtained from a Taiwanese Child: Evidence for a Relationship with Bovine Rotaviruses.

  Fang-Tzy Wu, Krisztián Bányai, Ho-Sheng Wu, Dustin Chen-Fu Yang, Jen-Shiou Lin, Chao Agnes Hsiung, Yhu-Chering Huang, Kao-Pin Hwang, Baoming Jiang, Jon R Gentsch
  Japanese journal of infectious diseases. 09/2012; 65(5):455-7.
• Article: Putative novel genotype of avian hepatitis E virus, Hungary, 2010.

  Krisztián Bányai, Ádám György Tóth, Éva Ivanics, Róbert Glávits, Katalin Szentpáli-Gavallér, Ádám Dán
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  ABSTRACT: To explore the genetic diversity of avian hepatitis E virus strains, we characterized the near-complete genome of a strain detected in 2010 in Hungary, uncovering moderate genome sequence similarity with reference strains. Public health implications related to consumption of eggs or meat contaminated by avian hepatitis E virus, or to poultry handling, require thorough investigation.
  Emerging Infectious Diseases 08/2012; 18(8):1365-8. · 6.79 Impact Factor
• Article: Detection of rare reassortant G5P[6] rotavirus, Bulgaria.

  Zornitsa Mladenova, Hajnalka Papp, György Lengyel, Péter Kisfali, Andrej Steyer, Adela F Steyer, Mathew D Esona, Miren Iturriza-Gómara, Krisztián Bányai
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  ABSTRACT: During the ongoing rotavirus strain surveillance program conducted in Bulgaria, an unusual human rotavirus A (RVA) strain, RVA/Human/BG/BG620/2008/G5P[6], was identified among 2200 genotyped Bulgarian RVAs. This strain showed the following genomic configuration: G5-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. Phylogenetic analysis of the genes encoding the neutralization proteins and backbone genes identified a probable mixture of RVA genes of human and porcine origin. The VP1, VP6 and NSP2 genes were more closely related to typical human rotavirus strains. The remaining eight genes were either closely related to typical porcine and unusual human-porcine reassortant rotavirus strains or were equally distant from reference human and porcine strains. This study is the first to report an unusual rotavirus isolate with G5P[6] genotype in Europe which has most likely emerged from zoonotic transmission. The absence of porcine rotavirus sequence data from this area did not permit to assess if the suspected ancestral zoonotic porcine strain already had human rotavirus genes in its genome when transmitted from pig to human, or, the transmission was coupled or followed by gene reassortment event(s). Because our strain shared no neutralization antigens with rotavirus vaccines used for routine immunization in children, attention is needed to monitor if this G-P combination will be able to emerge in human populations. A better understanding of the ecology of rotavirus zoonoses requires simultaneous monitoring of rotavirus strains in humans and animals.
  Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 07/2012; 12(8):1676-1684. · 3.22 Impact Factor
• Article: Surveillance of human rotaviruses in 2007-2011, Hungary: exploring the genetic relatedness between vaccine and field strains.

  Brigitta László, József Kónya, Eszter Dandár, Judit Deák, Ágnes Farkas, Jim Gray, Gábor Grósz, Miren Iturriza-Gomara, Ferenc Jakab, Ágnes Juhász, [......], Hajnalka Papp, László Pátri, Erzsébet Puskás, Ildikó Sántha, Ferenc Schneider, Katalin Szomor, András Tóth, Erzsébet Tóth, György Szűcs, Krisztián Bányai
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  ABSTRACT: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5 years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.
  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology 07/2012; 55(2):140-6. · 3.12 Impact Factor
• Article: Simian genogroup I picobirnaviruses: prevalence, genetic diversity, and zoonotic potential.

  Yuhuan Wang, Krisztián Bányai, Xinming Tu, Baoming Jiang
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  ABSTRACT: We previously reported the first detection of simian picobirnaviruses (PBVs) by polyacrylamide gel electrophoresis in fecal specimens of two monkeys with diarrhea in China. We now report the detection of genogroup I PBVs in 48% (44/92) of the fecal specimens by reverse transcriptase PCR (RT-PCR) and amplicon sequencing using primers specific for the RNA-dependent RNA polymerase (RDRP) gene. Molecular characterization of these 44 strains demonstrated both sequence conservation and diversity among simian PBVs and among simian, porcine, and human PBVs. We further determined full-length sequences of segment 2 of the two simian PBV strains, monkey/CHN-14/2002 and monkey/CHN-49/2002, and demonstrated 52.5% to 54.2% nucleotide sequence similarity to the corresponding gene of the bovine strain RUBV and the prototype human strain 1-CHN-97 of genogroup I PBVs and an even lower similarity (38.4%) to segment 2 of the prototype human genogroup II strain 4-GA-91. Further studies are needed to investigate the epidemiology and pathogenesis of PBVs in animals and humans.
  Journal of clinical microbiology 05/2012; 50(8):2779-82. · 4.16 Impact Factor
• Article: Systematic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era: insights for understanding the impact of rotavirus vaccination programs.

  Krisztián Bányai, Brigitta László, Jazmin Duque, A Duncan Steele, E Anthony S Nelson, Jon R Gentsch, Umesh D Parashar
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  ABSTRACT: Recently, two rotavirus vaccines have been recommended for routine immunization of infants worldwide. These vaccines proved efficacious during clinical trials and field use in both developing and developed countries, and appear to provide good protection against a range of rotavirus genotypes, including some that are not included in the vaccines. However, since conclusive data that the vaccines will protect against a wide variety of rotavirus strains are still lacking and since vaccines may exert some selection pressure, a detailed picture of global strain prevalence from the pre-rotavirus vaccine era is important to evaluate any potential changes in circulating strains observed after widespread introduction of rotavirus vaccines. Thus, we systematically reviewed rotavirus genotyping studies spanning a 12-year period from 1996 to 2007. In total, ~110,000 strains were genotyped from 100 reporting countries. Five genotypes (G1-G4, and G9) accounted for 88% of all strains, although extensive geographic and temporal differences were observed. For example, the prevalence of G1 strains declined from 2000 onward, while G3 strains re-emerged, and G9 and G12 strains emerged during the same period. When crude strain prevalence data were weighted by region based on the region's contribution to global rotavirus mortality, the importance of genotypes G1 and G9 strains that were more prevalent in regions with low mortality was reduced and conversely the importance of G8 strains that were more prevalent in African settings with greater contribution to global rotavirus mortality was increased. This study provides the most comprehensive, up-to-date information on rotavirus strain surveillance in the pre-rotavirus vaccine era and will provide useful background to examine the impact of rotavirus vaccine introduction on future strain prevalence.
  Vaccine 04/2012; 30 Suppl 1:A122-30. · 3.77 Impact Factor
• Article: Molecular characterization of a rare, human-porcine reassortant rotavirus strain, G11P[6], from Ecuador

  Krisztián Bányai, Mathew D. Esona, Tara K. Kerin, Jennifer J. Hull, Slavica Mijatovic, Nancy Vásconez, Carlos Torres, Ana M. B. de Filippis, Jon R. Gentsch
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  ABSTRACT: The Pan-American Health Organization established a rotavirus pre-vaccination disease burden and strain surveillance network in Latin America and the Caribbean in 2004. During strain surveillance in Ecuador in 2005–2006, a rare rotavirus genotype, G11P[6], was detected among common strains. Sequencing and phylogenetic analysis of this strain identified a novel lineage of the G11 VP7 gene, most closely related to A253 (91.8% nt identity), a porcine rotavirus strain identified in Venezuela. Most genes of this strain clustered with porcine, human-porcine or bovine-porcine reassortant strains; only VP6 and perhaps NSP2 genes were more closely related to cognate genes of human rotaviruses. Thus, this strain was likely generated by gene reassortment between porcine and human parental strains. Our study provides further evidence that animal rotaviruses play an important role in genetic and antigenic diversity of rotaviruses pathogenic for humans.
  Archives of Virology 04/2012; 154(11):1823-1829. · 2.11 Impact Factor
• Article: Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children.

  Kao-Pin Hwang, Fang-Tzy Wu, Krisztián Bányai, Ho-Sheng Wu, Dustin Chen-Fu Yang, Yhu-Chering Huang, Jen-Shiou Lin, Chao Agnes Hsiung, Jason C Huang, Baoming Jiang, Jon R Gentsch
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  ABSTRACT: The molecular characterization of genotype P[6] rotavirus strains collected from children admitted to hospital with acute dehydrating diarrhoea during a 6-year surveillance period in Taiwan is described in this study. In total, three G4P[6] strains, one G5P[6] and one G12P[6] were characterized by sequencing and phylogenetic analysis of the VP4, VP7, VP6 and NSP4 genes. Whilst all four genes of the single Taiwanese G12P[6] strain clustered with the respective genes of globally common human rotavirus strains, the G4 and G5 strains showed remarkable similarities to porcine rotavirus strains and putative porcine-origin human P[19] strains reported previously from Taiwan. The overall proportion of porcine rotavirus-like strains in Taiwan remains around 1 % among hospitalized children; however, the circulation and sporadic transmission of these heterotypic strains from pigs to humans could pose a public-health concern. Therefore, continuation of strain monitoring is needed in the vaccine era to detect any possible vaccine breakthrough events associated with the introduction of such heterologous rotavirus strains.
  Journal of Medical Microbiology 03/2012; 61(Pt 7):990-7. · 2.50 Impact Factor
• Article: Picobirnavirus infections: viral persistence and zoonotic potential.

  Balasubramanian Ganesh, Krisztián Bányai, Vito Martella, Ferenc Jakab, Gisela Masachessi, Nobumichi Kobayashi
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  ABSTRACT: Picobirnaviruses (PBVs) are small, non-enveloped, bisegmented double-stranded RNA genomic viruses of vertebrate hosts. Since their discovery in the late 1980s in clinical specimens from outbreaks of acute gastroenteritis in children, significant efforts have been made to investigate the role of PBV in diarrheic diseases. PBV has been detected in sporadic episodes of diarrhea as sole pathogen or coinfection as well as in outbreaks of acute gastroenteritis and in immunocompromised patients with diarrhea. However, PBV is frequently detected in non-diarrheic healthy hosts, and prolonged shedding has been observed in some individuals. Of interest, similar patterns of PBV infection have also been observed in pigs and other animal hosts. The increasing amount of PBV sequence data gathered from molecular epidemiological studies has evidenced a great sequence diversity of PBVs in various hosts and environmental samples. Importantly, evidence has been found for genetic relatedness between human and animal PBV strains, suggesting extant crossing points in the ecology and evolution of heterologous PBV strains. At present, no cell culture and animal model exists for PBVs. Well-structured epidemiological studies are still the only alternative to demonstrate the potential etiological role of PBVs in acute gastroenteritis or other diseases. This review aims to analyze the public health aspects of PBV infection, especially its possible association with zoonosis.
  Reviews in Medical Virology 02/2012; 22(4):245-56. · 7.20 Impact Factor
• Article: Putative canine origin of rotavirus strain detected in a child with diarrhea, Taiwan.

  Fang-Tzy Wu, Krisztián Bányai, Jen-Shiou Lin, Ho-Sheng Wu, Chao Agnes Hsiung, Yhu-Chering Huang, Kao-Pin Hwang, Baoming Jiang, Jon R Gentsch
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  ABSTRACT: Rotavirus G3P[3] strains have been reported from a variety of species including humans, cats, dogs, monkeys, goats, and cows. Here, we report the characterization of the first human G3P[3] rotavirus from East Asia identified in a 2-year-old child who was treated in a hospital's emergency ward in Taiwan in February 2005. Sequence and phylogenetic analysis demonstrated a close genetic relationship between the VP4, VP6, VP7, and NSP4 genes of Taiwanese G3P[3] strain 04-94s51 and an Italian canine strain isolated a decade ago, suggesting a canine origin for the Taiwanese strain. In contrast, the Taiwanese strain was only moderately related to well-characterized canine-origin human G3P[3] strains Ro1845 and HCR3, suggesting a distinct origin for the rotavirus strain from Taiwan.
  Vector borne and zoonotic diseases (Larchmont, N.Y.) 02/2012; 12(2):170-3. · 2.61 Impact Factor
• Article: Diversity and zoonotic potential of rotaviruses in swine and cattle across Europe.

  Sofie E Midgley, Krisztián Bányai, Javier Buesa, Nabil Halaihel, Charlotte K Hjulsager, Ferenc Jakab, Jérôme Kaplon, Lars E Larsen, Marina Monini, Mateja Poljšak-Prijatelj, Pierre Pothier, Franco M Ruggeri, Andrej Steyer, Marion Koopmans, Blenda Böttiger
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  ABSTRACT: Group A rotaviruses can infect both humans and animals. Individual rotavirus strains can occasionally cross species barriers and might hereby contribute to the emergence of new genotypes in heterologous hosts. The incidence and impact of zoonotic rotavirus are not well defined, and one reason for this is a lack of data about strains circulating in suspected reservoir animal hosts. In this study we report the incidence, genetic diversity, and molecular epidemiology of rotaviruses detected in domestic cattle and swine in 6 European countries. From 2003 to 2007, 1101 and more than 2000 faecal specimens were collected from swine and cattle, both healthy and diarrhoeic, and tested for rotaviruses. Viruses from positive stools were genotyped and a subset of strains was characterized by nucleotide sequencing and phylogenetic analysis of the VP7 (G) and VP4 (P) genes. Rotaviruses were detected in 43% of bovine samples and in 14% of porcine samples. In cattle, 10 different combinations of G and P types were identified and the most common strains were G6P[11] and G6P[5]. In swine, the number of identified G-P combinations was higher (n=21), however, no single combination was predominant across Europe. Newly described genotype specificities, P[27] and P[32], were identified in swine. When compared at the nucleotide sequence level, the identified porcine rotavirus strains and contemporary human strains grouped together phylogenetically, whereas bovine rotavirus strains formed separate clades. These data demonstrate large genetic diversity of porcine and bovine rotavirus strains across Europe, and suggest that livestock herds may serve as potential reservoirs for human infections.
  Veterinary Microbiology 10/2011; 156(3-4):238-45. · 3.33 Impact Factor
• Article: Diverse origin of P[19] rotaviruses in children with acute diarrhea in Taiwan: Detection of novel lineages of the G3, G5, and G9 VP7 genes.

  Fang-Tzy Wu, Krisztián Bányai, Jason C Huang, Ho-Sheng Wu, Feng-Yee Chang, Jyh-Yuan Yang, Chao Agnes Hsiung, Yhu-Chering Huang, Jen-Shiou Lin, Kao-Pin Hwang, Baoming Jiang, Jon R Gentsch
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  ABSTRACT: We previously reported the detection of genotype P[19] rotavirus strains from children hospitalized with acute dehydrating diarrhea during a 5-year surveillance period in Taiwan. The characterization of five P[19] strains (0.4% of all typed), including three G3P[19], a novel G5P[19], and a unique G9P[19] genotype is described in this study. Phylogenetic analysis of the VP4, VP7, VP6, and NSP4 genes was performed, which demonstrated novel lineages for respective genotypes of the VP4 and the VP7 genes. The sequence similarities of the P[19] VP4 gene among Taiwanese human strains was higher (nt, 91.5-96.2%; aa, 93.7-97.6%) than to other P[19] strains (nt, 83.5-86.6%; aa, 89.4-94.1%) from different regions of the world. The VP7 gene of the three G3P[19] Taiwanese strains shared up to 93.4% nt and 97.5% aa identity to each other but had lower similarity to reference strain sequences available in GenBank (nt, <90.1%; aa, <95.6%). Similarly, the VP7 gene of the novel G5P[19] strain was only moderately related to the VP7 gene of reference G5 strains (nt, 82.2-87.3%; aa, 87.0-93.1%), while the VP7 gene of the single G9P[19] strain was genetically distinct from other known human and animal G9 rotavirus strains (nt, ≤ 92.0%; aa, ≤ 95.7%). Together, these findings suggest that the Taiwanese P[19] strains originated by independent interspecies transmission events. Synchronized surveillance of human and animal rotaviruses in Taiwan should identify possible hosts of these uncommon human rotavirus strains.
  Journal of Medical Virology 07/2011; 83(7):1279-87. · 2.82 Impact Factor
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  Available from: Giovanni M Giammanco

  Article: Molecular characterization of genotype G6 human rotavirus strains detected in Italy from 1986 to 2009.

  Simona De Grazia, Vito Martella, Valentina Rotolo, Floriana Bonura, Jelle Matthijnssens, Krisztián Bányai, Max Ciarlet, Giovanni M Giammanco
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  ABSTRACT: Group A human rotavirus (HRV) strains with a bovine-like (G6) major outer capsid protein VP7 were first detected in Palermo, Italy, in the late 1980s, and subsequently worldwide. During a 25-year rotavirus surveillance period, additional HRV G6 strains, associated with either a P[9] or P[14] VP4 genotype, have been detected sporadically, but repeatedly, in Palermo. Whether these G6 HRVs were transmitted to humans directly from an animal reservoir or could have circulated at low prevalence in susceptible individuals is uncertain. Upon sequence analyses of the VP7, VP4, VP6, NSP4 and NSP5 gene segments, all the Italian HRV strains displayed a conserved genotype constellation, G6-P[9]/[14]-I2-E2-H3. Intra-genotypic lineages and/or sub-lineages were observed among the various HRV strains, with some lineage/sublineage combinations being retained over time. Interestingly, two epidemiologically unrelated G6P[9] viruses, collected in the same rotavirus season, were found to have a clonal origin. In conclusion, our results indicate not only diverse origin of animal derived G6 HRVs in Palermo but also suggest human-to-human transmission of certain strains.
  Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 05/2011; 11(6):1449-55. · 3.22 Impact Factor