Kazuhiro Asada

Publications (18)38.36 Total impact

• Article: Respiratory mechanics measured by forced oscillation technique in combined pulmonary fibrosis and emphysema.

  Kazutaka Mori, Toshihiro Shirai, Masashi Mikamo, Yuichiro Shishido, Takefumi Akita, Satoru Morita, Kazuhiro Asada, Masato Fujii, Hironao Hozumi, Takafumi Suda, Kingo Chida
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  ABSTRACT: The coexistence of emphysema and pulmonary fibrosis is known as combined pulmonary fibrosis and emphysema (CPFE). The aim of this study was to compare the lung mechanics measured by multi-frequency forced oscillation technique (FOT) among patients with CPFE, interstitial pneumonia (IP), and chronic obstructive pulmonary disease (COPD). FOT and pulmonary function tests were performed in 41 patients with CPFE, 47 with IP, and 86 with COPD. Whole-breath resistance at 20Hz was significantly lower in patients with CPFE than in those with IP or COPD, irrespective of the severity of airflow limitation. Within-breath analyses of resistance revealed no difference among the 3 groups; however, the difference between inspiratory and expiratory phases of reactance at 5Hz, which reflects expiratory flow limitation, in patients with CPFE was significantly higher than in those with IP and lower than in those with COPD. In conclusion, both emphysema and fibrosis affect lung mechanics in CPFE, leading to different findings from IP or COPD alone.
  Respiratory Physiology & Neurobiology 10/2012; · 2.24 Impact Factor
• Article: Impaired cough-related quality of life in patients with controlled asthma with gastroesophageal reflux disease.

  Toshihiro Shirai, Masashi Mikamo, Yuichiro Shishido, Takefumi Akita, Satoru Morita, Kazuhiro Asada, Masato Fujii, Kazutaka Mori, Takafumi Suda, Kingo Chida
  Annals of allergy, asthma & immunology: official publication of the American College of Allergy, Asthma, & Immunology 05/2012; 108(5):379-80. · 2.83 Impact Factor
• Article: Serum indoleamine 2,3-dioxygenase activity predicts prognosis of pulmonary tuberculosis.

  Yuzo Suzuki, Takafumi Suda, Kazuhiro Asada, Seiichi Miwa, Masako Suzuki, Michio Fujie, Kazuki Furuhashi, Yutaro Nakamura, Naoki Inui, Toshihiro Shirai, Hiroshi Hayakawa, Hirotoshi Nakamura, Kingo Chida
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  ABSTRACT: Tuberculosis (TB) continues to be a major health problem, and there are few biomarkers for predicting prognosis. Indoleamine 2,3-dioxygenase (IDO), a potent immunoregulatory molecule, catalyzes the rate-limiting step of tryptophan (Trp) degradation in the kynurenine (Kyn) pathway. An increase in IDO activity determined by the serum Trp/Kyn ratio has been shown to be associated with poor prognosis in cancers and bacteremia. In TB, however, there are no studies measuring serum IDO activity to determine its clinical significance. We evaluated serum IDO activity with 174 pulmonary TB (PTB) patients and 85 controls, using liquid chromatography/electrospray ionization tandem mass spectrometry. IDO activity was estimated by calculating the serum Kyn-to-Trp ratio. PTB patients had significantly higher Kyn concentrations and IDO activity and significantly lower Trp concentrations (P < 0.0001, P < 0.0001, and P < 0.0001, respectively) than the controls. Of 174 PTB patients, 39 (22.4%) died. The patients who died had significantly higher concentrations of Kyn and significantly lower Trp concentrations, resulting in significantly higher IDO activity (P < 0.0001, P < 0.0001, and P < 0.0001, respectively). In a receiver operating characteristic (ROC) analysis, serum IDO activity had the highest area under the curve (0.850), and this activity was an independent prognostic factor in multivariate analysis. These results suggest that serum IDO activity can be used as a novel prognostic marker in PTB.
  Clinical and vaccine immunology: CVI 01/2012; 19(3):436-42. · 2.37 Impact Factor
• Article: Colored 3-dimensional analyses of respiratory resistance and reactance in COPD and asthma.

  Kazutaka Mori, Toshihiro Shirai, Masashi Mikamo, Yuichiro Shishido, Takefumi Akita, Satoru Morita, Kazuhiro Asada, Masato Fujii, Takafumi Suda, Kingo Chida
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  ABSTRACT: The forced oscillation technique (FOT) is a noninvasive method with which to measure respiratory system resistance (Rrs) and reactance (Xrs) at a wide range of frequencies during breathing at rest in a short time. The purpose of this study was to assess the differences in Rrs and Xrs between patients with chronic obstructive pulmonary disease (COPD) and asthma using a new method of FOT with colored 3-dimensional visualization. Fifty-one patients with stable COPD and 49 patients with controlled or partly controlled asthma were enrolled. Whole-breath or within-breath changes of Rrs and Xrs were measured and compared between the diseases. The colored 3-dimensional images clarified the complex oscillatory properties of the respiratory system. Whole-breath resistance (the difference in Rrs at 5 and 20 Hz) and reactance (Xrs at 5 Hz and resonant frequency), and within-breath changes in reactance (Xrs at 5 Hz and resonant frequency) discriminated between patients with COPD and asthma. In multivariate regression analyses, inspiratory-expiratory differences in Xrs at 5 Hz contributed significantly to the differentiation between COPD and asthma, independent of age, gender, body weight, and pulmonary function. This new method of FOT is useful in the differential diagnosis of COPD and asthma.
  COPD Journal of Chronic Obstructive Pulmonary Disease 12/2011; 8(6):456-63. · 1.79 Impact Factor
• Article: Elevated alveolar nitric oxide concentration after environmental challenge in hypersensitivity pneumonitis.

  Toshihiro Shirai, Masaki Ikeda, Satoru Morita, Kazuhiro Asada, Takafumi Suda, Kingo Chida
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  ABSTRACT: We describe a 57-year-old male patient admitted to hospital with hypersensitivity pneumonitis (HP) that resolved without treatment. The total and alveolar nitric oxide (NO) concentrations were measured on initial admission and after re-exposure to his home environment. Following environmental exposure he became ill again, alveolar NO concentration was increased to the same level as on initial admission and impaired pulmonary function and radiologic abnormalities were found. It suggested a diagnosis of environmentally induced HP. The clinical value of measuring alveolar NO as an acute-phase reactant in HP is demonstrated in this patient.
  Respirology 03/2010; 15(4):721-2. · 2.42 Impact Factor
• Article: [A case of psittacosis showing a localized peripheral air-space consolidation].

  Masato Karayama, Naoki Inui, Hideki Yasui, Sawa Yamazaki, Eriko Muramatsu, Tomohiro Uto, Satoru Morita, Kazuhiro Asada, Tomoyoshi Tsutiya, Yutaka Nakano, Takafumi Suda, Kingo Chida
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  ABSTRACT: A 29-year-old man was admitted with fever and anorexia. Radiographic examinations of the chest showed a localized peripheral non-segmental air-space consolidation in the right lower lobe. He had a history of exposure to parakeets, and psittacosis was diagnosed based on the elevated serum complement fixation titer against Chlamydia psittaci. The common radiographic finding of psittacosis is ground-glass attenuation radiating from the hilar areas. We report a rare case of psittacosis presenting a localized consolidation, clearly limited to the subpleural region of the lung.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 10/2006; 44(9):670-3.
• Article: Suplatast tosilate alters DC1/DC2 balance in peripheral blood in bronchial asthma.

  Koushi Yokomura, Takafumi Suda, Hiroyuki Matsuda, Hideo Hashizume, Kazuhiro Asada, Kenichiro Suzuki, Kingo Chida
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  ABSTRACT: Suplatast tosilate is an antiallergic drug that selectively suppresses Th2-cytokine production and inhibits airway hyperresponsiveness and eosinophilic airway inflammation. This drug has been also shown to improve pulmonary function and symptoms in steroid-dependent asthma, resulting in a decrease in doses of inhaled corticosteroid. However, the precise mechanism by which suplatast tosilate exerts an antiasthmatic effect in vivo remains to be known. Our previous study showed the polarization of circulating type 1 dendritic cells (DC1)/type 2 dendritic cells (DC2) balance toward DC2 in asthma, which might be associated with its Th2-dominant immune response. In the present study, we attempted to clarify the effect of suplatast tosilate on DC1/DC2 balance in asthma. Using multicolor flow cytometry, we enumerated circulating DC1 and DC2 before and 8 weeks after treatment with suplatast tosilate in nine patients with asthma. Before the treatment, the patients with asthma had a significant higher percentage of DC2 together with a significant lower ratio of DC1/DC2 compared with normal subjects. Administration of suplatast tosilate significantly decreased the percentage of DC2, but not that of DC1, resulting in a significant raises of the ratio of DC1/DC2. Concomitantly, intracellular cytokine analysis showed that the percentage of IL-4 producing CD4+ T cells was significantly decreased after the treatment. These data suggest that suplatast tosilate normalizes the polarized DC1/DC2 balance toward DC2 in asthma, which may also alter its Th2-dominant Th1/Th2 balance toward Th1.
  Journal of Asthma 10/2005; 42(7):567-70. · 1.52 Impact Factor
• Article: Antiinflammatory roles of peroxisome proliferator-activated receptor gamma in human alveolar macrophages.

  Kazuhiro Asada, Shigekazu Sasaki, Takafumi Suda, Kingo Chida, Hirotoshi Nakamura
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  ABSTRACT: Peroxisome proliferator-activated receptor gamma (PPARgamma) is a ligand-activated transcriptional factor belonging to the nuclear receptor superfamily. PPARgamma, which is predominantly expressed in adipose tissue, plays a major regulatory role in glucose metabolism and adipogenesis. Interestingly, recent studies have demonstrated PPARgamma expression in monocytes/macrophages and its antiinflammatory activities. However, it is unclear whether alveolar macrophages (AMs) express functional PPARgamma. The present study was conducted to investigate the expression of PPARgamma by AMs and to elucidate its functional role. Using reverse transcription-polymerase chain reaction and Western blotting, we demonstrated the strong expression of PPARs messenger RNA and protein in freshly isolated human AMs. Ligands of PPARgamma, 15-deoxy-delta(12,14)prostaglandin J2, and troglitazone significantly decreased LPS-induced tumor necrosis factor-alpha production by AMs. These ligands markedly upregulated the expression of CD36, a scavenger receptor that mediates the phagocytosis of apoptotic neutrophils. Indeed, ligand-treated AMs ingested a significantly higher number of apoptotic neutrophils than untreated AMs. These data indicate that PPARgamma expressed by AMs play an antiinflammatory role through inhibiting cytokine production and increasing their CD36 expression together with the enhanced phagocytosis of apoptotic neutrophils, which is an essential process for the resolution of inflammation. This suggests the potential therapeutic application of PPARgamma ligands in inflammatory disorders of the lung.
  American Journal of Respiratory and Critical Care Medicine 02/2004; 169(2):195-200. · 11.08 Impact Factor
• Article: [An autopsy case of cor pulmonale due to a pulmonary tumor embolism as the first clinical manifestation of occult gastric cancer].

  Hiroyuki Matsuda, Kingo Chida, Seiiti Miwa, Hideki Nakano, Hirofumi Kuwata, Kenichiro Suzuki, Koshi Yokomura, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Takafumi Suda, Hirotoshi Nakamura, Yasuhisa Naito, Haruhiko Sugimura
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  ABSTRACT: A 47-year-old man was admitted to our hospital because of progressive dyspnea and cough. Physical examination and chest radiographs showed the signs of cor pulmonale. A lung scan using perfused radionuclide revealed multiple peripheral perfusion defects and catheterization of the right heart showed severe pulmonary hypertension. A diagnosis of severe pulmonary embolism was made. Despite intensive care with anti-coagulation therapy, the patient died on the third-hospital day. Autopsy disclosed gastric cancer in the pylorus with metastases to the regional lymph nodes. There were no macroscopic pulmonary artery emboli or parenchymal lesions, but more than 60% of the small arteries and arterioles were occluded by casts of tumor cells. Cor pulmonale due to a pulmonary tumor embolism is a rare complication of cancer. This case is particularly unusual because the embolus-caused cor pulmonale was the initial manifestation of clinically occult, but pathologically advanced, gastric cancer.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 12/2002; 40(11):910-4.
• Article: Alteration of balance between myeloid dendritic cells and plasmacytoid dendritic cells in peripheral blood of patients with asthma.

  Hiroyuki Matsuda, Takafumi Suda, Hideo Hashizume, Koushi Yokomura, Kazuhiro Asada, Kenichiro Suzuki, Kingo Chida, Hirotoshi Nakamura
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  ABSTRACT: Asthma, a well-known helper T cell Type 2 (Th2)-mediated disease, has a polarized immune response toward a Th2 phenotype. However, the factors causing the Th2 polarization remain to be fully determined in this disease. Dendritic cells (DCs) are the most potent antigen-presenting cells that play a central role in initiating the primary immune response. In human blood, two functional distinct subsets of DCs, myeloid DCs and plasmacytoid DCs, have been identified. Myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) are also called Type 1 DCs (DC1) and Type 2 DCs (DC2), respectively, because mDCs and pDCs were shown to preferentially differentiate naive T cells into Th1 and Th2 cells, respectively. In asthma, it can thus be speculated that an altered balance of mDCs to pDCs toward pDCs may contribute to the Th2 polarization. To clarify this, we examined the numbers of mDCs and pDCs in the peripheral blood of 44 patients with asthma and 38 normal subjects, using multicolor flow cytometry. We found that the patients with asthma had a significantly higher number of pDCs, resulting in a significant decrease in the ratio of mDCs to pDCs compared with normal subjects. These data indicate that the patients with asthma had a polarization of the mDC:pDC balance toward pDCs, which may be involved in producing the Th2-dominant immune phenotype in asthma.
  American Journal of Respiratory and Critical Care Medicine 11/2002; 166(8):1050-4. · 11.08 Impact Factor
• Article: [Clinical analysis of chronic hypersensitivity pneumonitis].

  Seiichi Miwa, Kyoutarou Ide, Hideki Nakano, Hiroshi Kuwata, Kenichirou Suzuki, Hiroyuki Matsuda, Kouji Yokomura, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Masahiro Shirai, Takafumi Suda, Kingo Chida, Hirotoshi Nakamura
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  ABSTRACT: Chronic hypersensitivity pneumonitis (CHP) has a poor prognosis because of the difficulties in its diagnosis and treatment. Recently, we have encountered six cases of CHP (4 patients with the home-related type, a patient with bird fancier's lung, and one with flour-induced CHP), and we examined the clinical characteristics of these cases. Environmental provocation testing has been considered useful for diagnosing HP, but all patients gave negative results in short-term environmental exposure tests performed routinely for the diagnosis of HP. However, 5 patients had a positive response in long-term environmental exposure tests. Two patients died of respiratory failure after ten years' observation despite improvement of the causative environment, and were given steroid therapy. Radiographically and pathologically, the process of progressive and irreversible lesions were recognized in our series of CHP patients. The diagnosis of CHP should be carried out on the basis of the comprehensive findings including the detailed history, clinical course, radiography, and histology.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 10/2002; 40(9):732-7.
• Article: [Prognosis and prognostic factors of sarcoidosis in Japan].

  Kazuhiro Asada, Kingo Chida
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  ABSTRACT: Sarcoidosis is a systemic granulomatous disease that primarily affects the lung and lymphatic systems. Sarcoidosis patients commonly have spontaneous remission and good prognosis. But some patients show chronic, progressive and life-threatening conditions. Determination of prognosis based on the initial clinical presentation is important for the treatment. In this article, we review natural history, prognosis and the factors related prognosis of sarcoidosis patients in Japan. Shadows on chest radiograph were cleared in 76% of patients for 10 years. Approximately 15% of patients had severe organ dysfunction. In 60% of the sarcoidosis autopsy cases, the causes of death were attributed to organ involvement of sarcoidosis. Adverse prognostic factors are advanced age at onset, the presence of symptoms, extrathoracic involvement and treatment of corticosteroids.
  Nippon rinsho. Japanese journal of clinical medicine 10/2002; 60(9):1839-44.
• Article: [Secondary pulmonary alveolar proteinosis associated with myelodysplastic syndrome].

  Koshi Yokomura, Kingo Chida, Takafumi Suda, Seiichi Miwa, Hideki Nakano, Hirofumi Kuwata, Kenichiro Suzuki, Hiroyuki Matsuda, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Masahiro Shirai, Kazuya Suzuki, Hirotoshi Nakamura
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  ABSTRACT: A 51-year-old man with myelodysplastic syndrome (MDS) was admitted to our hospital because of dyspnea on exertion in December 1999. Chest radiography showed ground-glass shadows in the middle and lower fields of both lungs, and chest computed tomography revealed a typical "crazy paving appearance". The bronchoalveolar lavage fluid was milky in appearance, and so secondary pulmonary alveolar proteinosis associated with MDS was diagnosed. Because there was no need to treat his MDS, we twice performed whole-lung lavage under general anesthesia in January and February 2000. The treatments were effective, and his abnormal chest radiography findings, laboratory data and pulmonary function were normalized. This was a rare case of secondary pulmonary alveolar proteinosis associated with MDS successfully treated with whole-lung lavage.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 08/2002; 40(7):599-604.
• Article: [Eosinophilic pleural effusion associated with dantrolene administration].

  Koshi Yokomura, Kingo Chida, Takafumi Suda, Seiichi Miwa, Hideki Nakano, Hirofumi Kuwata, Kenichiro Suzuki, Hiroyuki Matsuda, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Toshihiro Shirai, Kazuya Suzuki, Hirotoshi Nakamura
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  ABSTRACT: A 42-year old man was admitted to our hospital for evaluation of pleural effusion in the right hemithorax. He had been treated for spastic paraplegia with dantrolene sodium for 28 months. The pleural fluid consisted of sterile exudates with a very high eosinophil count, and peripheral blood eosinophilia was noted. Thoracoscopy revealed no apparent abnormalities and a pleural biopsy specimen showed only non-specific inflammation. Two weeks after discontinuing dantrolene therapy, the pleural effusion disappeared. The toxicity of dantrolene in patients with pleural effusion must be taken into consideration when long-term dantrolene therapy is given.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 07/2002; 40(6):503-7.
• Article: Oncostatin M production by human dendritic cells in response to bacterial products.

  Takafumi Suda, Kingo Chida, Akihito Todate, Kyotaro Ide, Kazuhiro Asada, Yutaro Nakamura, Kenichiro Suzuki, Hirofumi Kuwata, Hirotoshi Nakamura
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  ABSTRACT: Oncostatin M (OSM) is a pleiomorphic cytokine that belongs to the IL-6 cytokine family. It is produced by activated T cells and monocytes/macrophages and plays an important role in the process of inflammatory responses. Although dendritic cells (DCs) have been shown to secrete a variety of cytokines, it is not elucidated whether DCs are able to produce OSM. To clarify this, using human DCs derived from peripheral blood cells, we measured the protein levels of OSM in the supernatants of DC cultures by ELISA and examined the expression of OSM mRNA by RT-PCR after stimulation with lipopolysaccharide (LPS) or fixed Staphylococcus aureus (SACS). Upon stimulation with bacterial products, DCs secreted a large amount of OSM protein in a dose- and time-dependent manner. Concomitantly, the expression of OSM mRNA by DCs was markedly up-regulated. Compared the ability of DCs to produce OSM with that of monocytes, which are major producers of OSM, DCs released significantly higher amounts of OSM protein in the culture supernatants than monocytes. These findings indicate for the first time that human monocyte-derived DCs can synthesize and secrete large amounts of OSM in response to bacterial products, suggesting that OSM produced by DCs at infectious sites may play a role in modulating inflammatory responses.
  Cytokine 04/2002; 17(6):335-40. · 3.02 Impact Factor
• Article: [Recurrence of inflammatory pseudotumor of the lung with endobronchial lesion after resection].

  Koshi Yokomura, Kingo Chida, Takafumi Suda, Hirofumi Kuwata, Kenichiro Suzuki, Hiroyuki Matsuda, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Tomoyoshi Tsuchiya, Kazuya Suzuki, Hirotoshi Nakamura
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  ABSTRACT: A 74-year-old man was admitted to our hospital because of an abnormal shadow in the left middle lung field on chest radiography. Since a transbronchial biopsy was not diagnostic, a left lower lobectomy was performed. Histopathologically, the patient was diagnosed as having an inflammatory pseudotumor of the lung. Four months after the surgery, the patient was readmitted with hemosputum. Bronchoscopically, an endobronchial lesion was found in the left main bronchus. Transbronchial biopsy of the lesion showed proliferation of inflammatory cells compatible with inflammatory pseudotumor. Therefore, a recurrent inflammatory pseudotumor was diagnosed. Treatment with corticosteroid resulted in the disappearance of the endobronchial lesion and the erythrocyte sedimentation rate (ESR) returned to normal. The patient showed a prolonged elevation of ESR even after the resection, suggesting that the ESR may be a good indicator of the activity of this disease.
  Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 03/2002; 40(2):129-34.
• Article: F-13 当院におけるCT上径2cm以下の小結節影切除症例の検討

  祐太郎 中村, 政敏 岩田, 倫博 堀口, 諭 大井, 三郎 小田, 和博 朝田, 秀樹 中野, 金吾 千田, 篤彦 佐藤, Yutaro Nakamura, Masatoshi IWATA, Tomohiro HORIGUCHI, Satoshi OHI, Kazuhiro Asada, Hideki Nakano, Kingo Chida, Atsuhiko SATO
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  ABSTRACT: rights: 日本肺癌学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation：isVersionOf: http://ci.nii.ac.jp/naid/110003123949/
• Article: 19.脈絡膜転移による視力障害にて発症した肺癌の1例(第76回日本肺癌学会中部支部会)

  宏幸 松田, 光司 横村, 和博 朝田, 祐太郎 中村, 直輝 乾, 智義 土屋, 潤 佐藤, 亮人 戸舘, 隆文 須田, 金吾 千田, Koshi Yokomura, Kazuhiro Asada, Yutaro Nakamura, Naoki Inui, Tomoyoshi TSUTIYA, Akihito Todate, Takafumi Suda, Kingo Chida
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  ABSTRACT: rights: 日本肺癌学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation：isVersionOf: http://ci.nii.ac.jp/naid/110003111607/