M Gabrashanska

Bulgarian Academy of Sciences, Ulpia Serdica, Sofia-Capital, Bulgaria

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Publications (24)26.97 Total impact

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    ABSTRACT: This study was undertaken to determine the dietary supplements of Zn containing diet on the antioxidant status in chickens experimentally infected with Eimeria acervulina. The antioxidant status was monitored via determination of MDA concentrations and erythrocyte SOD and CAT activities, as well as vitamin E, vitamin C, Cu, and Zn in liver, muscle, and serum. The results showed increased MDA (P < .05), CAT (P < .001), and decreased SOD (P < .001) in the infected birds. Significant changes in Cu and Zn concentrations and dramatically reduction of vitamin C and E concentrations in the infected chickens were found. The observed deviations in the studied enzymes and nonenzymatic parameters evidence the occurrence of oxidative stress following the infection and impaired antioxidant status of chickens, infected with Eimeria acervulina. Our results proved the ameliorating role of CuZn(OH)(3)Cl (0.170 g per kg food) against Eimeria acervulina-induced oxidative damage in infected chickens.
    03/2011; 2011:647124. DOI:10.4061/2011/647124
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    Georgieva N.V. · M. Gabrashanska · V. Koinarski · S. Ermidou-Pollet
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    ABSTRACT: The effect of sodium selenite on the antioxidant status in broiler chickens infected with Eimeria acervulina was studied. Antioxidant status was measured via blood plasma malonyl dialdehyde (MDA), serumglutathione peroxidase (GPx), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities and plasma levels of selenium (Se), vitamins A, C and E with respect to parasitological and production traits. The study was performed on 100, 20-day old broiler chickens. Four groups of chickens were formed: the 1st – control, the 2nd – control and receivedNa2SeO3 (0.3mg/kg diet for 10 days), the 3rd – infected with 3 × 105 sporulated E. acervulina oocysts and the 4th –infected with E. acervulina oocysts and received the same dose Na2SeO3. Blood samples were taken on Day 8 after the first inoculation. Increased MDA and CAT, reduced SOD and GPx and Se content, and hypovitaminoses A, C and E in the infected chicks, compared to the healthy controls were observed. The changes in the small intestine, lesion scores and oocyst index, and body weight were indicative for a severe E. acervulina infection. Inorganic Se supplementation increased vitamin E, Se content, pGPx-activity, and improved body weights, but it did not influence the parasitological indices.
    Trace Elements and Electrolytes 01/2011; 28(1):42-48. DOI:10.5414/TEP28042 · 0.45 Impact Factor
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    ABSTRACT: Selenium (Se) supplementation may prevent the formation of free radicals and lipid peroxidation processes in trichinellosis. The oxidative-antioxidant status of male Wistar rats infected or uninfected with Trichinella spiralis (Nematoda) and supplemented or unsupplemented with Sel-plex (Alltech) was tested through blood biomarkers. Sel-plex was applied to restore antioxidant defense system. The oxidative marker was malondialdehyde (MDA) concentration. The antioxidant markers were superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and concentrations of Se and vitamin E. The animals were allocated into four groups. The experiment covered 8 weeks post infection. A mathematical model was proposed for the time course of host body weight. The model solutions were in good agreement with the experimental data. The relative rates of body weight gain were determined as growth kinetic parameters. The supplementation of the rats with dietary Se improved their antioxidant status. Increases by 10% in SOD activity, 6% in GPx activity, 13% in vitamin E concentration, 17% in plasma Se concentration, and 19% in liver Se concentration, respectively, and a decrease by 18% in serum MDA concentration were recorded in the infected and supplemented towards infected and unsupplemented rats. The reduction of muscle larvae after Sel-plex application was 63%. The mortality in infected and uninfected animals did not differ significantly. No statistically significant differences were established between the growth of the control and infected rats. At week 8, the body weight gain in the supplemented rats (both uninfected and infected) was 30% higher, compared to that in unsupplemented ones. Diet with Se could be beneficial in the treatment of diseases correlated with considerable oxidative stress, particularly parasitoses.
    Parasitology Research 02/2010; 106(3):561-70. DOI:10.1007/s00436-009-1695-3 · 2.33 Impact Factor
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    ABSTRACT: The effect of sodium selenite on the antioxidant status in broiler chickens infected with Eimeria acervulina was studied. Antioxidant status was measured via blood plasma malonyl dialdehyde (MDA), serum glutathione peroxidase (GPx), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities and plasma levels of selenium (Se), vitamins A, C and E with respect to parasitological and production traits. The study was performed on 100, 20-day old broiler chickens. Four groups of chickens were formed: the 1st – control, the 2nd – control and received Na2SeO3(0.3 mg/kg diet for 10 days), the 3rd - infected with 3x105 sporulated E. acervulina oocysts and the 4th – infected with E. acervulina oocysts and received the same dose Na2SeO3. Blood samples were taken on the day 8 after the first inoculation. Increased MDA and CAT, reduced SOD and GPx and Se content, and hypovitaminoses A, C and E in the infected chicks, compared to the healthy controls were observed. The changes in the small intestine, lesion scores and oocyst index, and body weight were indicative for a severe E. acervulina infection. Inorganic Se supplementation increased vitamin E and Se content and pGPx-activity, improved body weights but it did not influence the parasitological indices.
    7th. International Symposium on Trace Elements in Human: New Perspectives; 01/2009
  • Margarita Gabrashanska · Svetla E Teodorova · Milena Anisimova
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    ABSTRACT: The oxidative-antioxidant status of male Wistar rats infected or uninfected with Fasciola hepatica (Trematoda) and supplemented or unsupplemented with 2Gly.ZnCl(2).2H(2)O was tested through liver biomarkers. The oxidative marker was malondialdehyde concentration. The antioxidant markers were glutathione peroxidase activity and concentrations of zinc, selenium, vitamin A, vitamin C, and vitamin E. The animals were allocated into four groups. The experiment covered 8 weeks post infection. The gain in the host body weight and rats' mortality were also studied. A mathematical model was elaborated to describe the kinetics of concentrations of liver zinc. The kinetic parameters calculated reflect the peculiarities in zinc absorption in different conditions. Also, a model was proposed for the time course of host body weight. The model solutions were in good agreement with the experimental data. The supplementation of the rats with dietary Zn improved their antioxidant status. Increases by 9% in GPX activity, 6% in Vitamin A concentration, 3% in Vitamin C concentration, 35% in Vitamin E concentration, 17% in liver Zn concentration, and 11% in liver Se concentration, respectively, and a decrease by 30% in liver MDA concentration were recorded in the infected and supplemented towards infected and unsupplemented rats. Also, an increase by 6% in body weight in supplemented animals was established.
    Parasitology Research 09/2008; 104(1):69-78. DOI:10.1007/s00436-008-1160-8 · 2.33 Impact Factor
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    ABSTRACT: The activity of selenium-dependent glutathione peroxidase (GPX), liver concentration of vitamin E, and plasma and liver selenium levels were used for estimation of the antioxidant status of broiler chickens infected with Ascaridia galli. These biomarkers were recorded in an experiment covering 70 days p.i. At the same time the establishment rate of A. galli in chicken intestines, gain in the host body weight and chicken survival were studied. Broiler chickens (Cobb hybrids) were infected with 1450 embryonated A. galli eggs and treated with Sel-plex. A mathematical model was applied to determine the rate of nematode reduction and the relative rate of gain of host body weight, which are essential kinetic parameters of parasite-host interaction. The activity of GPX increased with both elevated selenium and reduced infection levels. The concentrations of selenium and vitamin E, and the GPX activity in the infected chickens demonstrated a similar pattern of change with time after day 30 p.i. The supplementation of the broilers with dietary selenium in the form of Sel-plex improved their antioxidant status. Increases by 29% in vitamin E concentration, 15% in GPX activity, and 22% in liver selenium concentration, respectively, were recorded in the infected and treated, compared to infected and untreated broilers.
    Journal of Helminthology 01/2008; 81(4):399-408. DOI:10.1017/S0022149X07868787 · 1.30 Impact Factor
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    ABSTRACT: The effect of selenium–yeast (Sel-plex 50, Alltech) on the antioxidant defense system (vitamins E, C, A, and Se content and Se-GPx-activity) in chicks experimentally infected with Eimeria tenella (Protozoa) was studied by us. The study was performed on 100 broiler chicks divided into four groups: first group - control, second - control and supplemented with Selplex, third group - infected with E. tenella, and fourth group - infected with E. tenella and supplemented with Sel-Plex. The chicks were fed a standard diet without antibiotics and coccidiostats. The second and fourth groups were fed on the diet to which Sel-Plex 50 was added - 0.3 mg/kg diet. The third and fourth groups were infected with 10.84 sporulated E. tenella oocysts. Serum and liver Se content was determined by a fluorimetric method. The content of liver vitamins C, E, and A was established by HPLC systems. Serum Se-GPx activity was determined spectrophotometrically. An antioxidant imbalance was developed due to the E. tenella infection. It was expressed by reduced vitamins A, C, and E levels, and Se content and reduced GPx activity. The Sel-Plex administration almost restored vitamin E and Se losses and increased GPx activity in the infected chickens. The observed changes in the small intestine, lesions, and oocyst indices, body weight gain, and feed conversion ratio were indicative of a severe E. tenella infection. They were correlated with oxidative stress. Administration of Sel-Plex resulted in significant increase in weight gain, improved feed conversion ratios, and increased GPx activity, Se, and vitamin E liver level in the control and infected chicks. The oocyst and lesion indices were slightly reduced after the treatment in the infected chicks. The experiment was approved by the Committee on Animal Experimentation at Trakia University, Stara Zagora, Bulgaria and was performed according to the recommendations of the Directive 86/609/EC.
    Cell Biology and Toxicology 01/2008; · 1.97 Impact Factor
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    ABSTRACT: Cited By (since 1996):2, Export Date: 18 October 2014
    Trace Elements and Electrolytes 01/2008; 25(4):199-205. DOI:10.5414/TEP25199 · 0.45 Impact Factor
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    ABSTRACT: Cited By (since 1996):1, Export Date: 18 October 2014
    Trace Elements and Electrolytes 01/2007; 24(1):61-66. DOI:10.5414/TEP24061 · 0.45 Impact Factor
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    V. KOINARSKI · M. GABRASHANSKA · N. GEORGIVA · P. PETKOV
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    ABSTRACT: The effect of 2Gly.ZnCl2.2H2O compound on the antioxidant status in chicks infected experimentally with Eimeria acervulina was studied. Antioxidant status was measured via determination of blood plasma malonyl dialdehyde (MDA) reactive products, activity of superoxide dismutase (SOD) and catalase (CAT) as well as blood concentrations of carotene, vitamins A, C, and E, and zinc. The results showed increased MDA and CAT-activity, decreased SOD activity, hypovitaminosis C, A and E, and reduced Znlevel in the infected chicks. An antioxidant imbalance was developed due to the E. acervulina infection. The 2Gly.ZnCl2.2H2O oral administration restored vitamin E and zinc losses, and reduced CAT-activity. However, SOD activity, vitamins C and A, carotene and MDA levels in the infected chicks were not statistically changed. The observed changes in the small intestine, lesion and oocyst index, and economical parameters (body weight gain and feed conversion ratio) were indicative for a severe E. acervulina infection. They were correlated with the oxidative stress. Administration of 2Gly.ZnCl2.2H2O increased body weight gain of the infected chicks. The oocyst and lesion indices as well as the feed conversion ratio did not respond to the treatment. 2Gly.ZnCl2.2H2O enhanced the antioxidant balance and performance of chicks with eimeriosis.
    Bulletin- Veterinary Institute in Pulawy 01/2006; 50(1):55-61. · 0.37 Impact Factor
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    V. Koinarski · N. Georgieva · M. Gabrashanska
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    ABSTRACT: The effect of a newly synthesized Zn salt (2Gly.ZnCl2.2H2O) co-administered with an antioxidant drug, maduramycin, on the antioxidant status of broiler chickens infected with E. tenella were investigated through measurements of plasma MDA (malondialdehyde) concentrations and of erythrocyte SOD (Superoxide Dismutase) and CAT (Catalase) activities. A total of 80 healthy Cobb 500 chickens were divided into 4 groups. In the group I, animals were not infected and not treated (negative controls). In the 3 other groups, birds, aged of 13 days, were infected with E. tenella (8.104 oocysts) and were not treated (group II, positive controls) or were orally treated for 9 days by only maduramycin (5 ppm) (group III) and by maduramycin (5 ppm) and Zn salt (2Gly.ZnCl2.2H2O) (0.12g Zn2+/kg of food/day) (group IV). A markedly stunted grouth and increase of FCR (food conversion ratio) were observed in positive controls, whereas the 2 treatments partially reduced the adverse effects of the E. tenella infection on growth. Compared to the negative controls, SOD activities were significantly reduced and CAT activities enhanced in all infected birds, and these changes were more intense in the 2 treated birds, particularly in birds receiving maduramycin and Zn salt. However, contrary to therapy with maduramycin alone, the cotreatment with Zn salt did not limit ROS formation: in this group, plasma MDA concentrations remained significantly elevated and similar to values observed in positive controls. These results confirm that the Eimeria tenella infection induces an oxidative stress in broiler chickens and that antioxidant therapy (maduramycin ± Zn salt) partially restored the growth, but they also suggest that Zn salt (2Gly.ZnCl2.2H2O) may present adverse effects on lipid peroxidation.
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    V. KOINARSKI · M. GABRASHANSKA · N. GEORGIVA · P. PETKOV
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    ABSTRACT: The antioxidant status of broiler chickens (Cobb 500 hybrids) experimentally infected with E. acervulina was monitored via determination of blood plasma malonyl dialdehyde (MDA) reactive products, the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as blood carotene, vitamin A and vitamin C concentrations. The results of the experiment showed a statistically significant increase of MDA concentrations (a marker of radical-induced damage) in E. acervulina-infected birds compared to healthy chickens (p<0.05). A decreased SOD activity was also observed in infected birds (p<0.001), whereas significant increase of CAT activities were obtained (p<0.001). Carotene, vitamin A and vitamin C concentrations were dramatically reduced in infected chickens (p<0.001, p<0.01 and p<0.05 respectively). The observed deviations in studied enzymes and non-enzymatic parameters evidence the occurrence of oxidative stress following the infection and impaired antioxidant status of broiler chickens infected with E. acervulina. The observed changes in small intestine, the oocyst production and the economical parameters (weight gain and feed conversion ratio) were indicative for a severe infection, in which the oxidative stress was also involved during pathogenesis.
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    ABSTRACT: Hisex chickens were infected with 1,450 embryonated Ascaridia galli eggs and treated with a new synthesized basic mixed salt (Zn(x)Co(y)Mn(1-x-y)) x (OH)6SO4 x 2H2O). The worm burden was determined and sex ratios for A. galli of M:F = 1.4 and M:F = 2 in untreated and treated chickens, respectively, were found. A decrease in the mean establishment rate of A. galli in treated chickens was observed. The levels of zinc, cobalt and manganese were determined in liver and muscle of the host and in male and female A. galli. The survival of the chickens and gain in body weight were improved, and the restoration of microelement content was observed by treatment with the salt. A positive effect of the basic Zn-Co-Mn salt was also observed in the nematode microelement levels. Significant differences were found between the levels of zinc, cobalt and manganese in male and female A. galli.
    Parasitology Research 07/2004; 93(3):242-7. DOI:10.1007/s00436-004-1117-5 · 2.33 Impact Factor
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    ABSTRACT: A newly synthesized basic mixed salt (Zn(x)Co(y)Mn(1-x-y)) x (OH)6SO4 x 2H2O) was administered to chickens with ascaridiosis. Improvement in survival, gain in body weight (of 19.03%) and restoration of microelement content were observed in the treated chickens. An increase in the gain in body weight of 7.62% in uninfected treated chickens was also observed. The establishment of Ascaridia galli populations in chickens, and chicken growth in control and infected hosts, untreated and treated, were modelled mathematically. Some kinetic parameters (the rate of reduction of the nematode population nu and the relative rate mu of gain in body weight of the host) were determined. The values of nu =0.027 day(-1) and nu* =0.032 day(-1) were calculated for the reduction rates in infected, untreated chickens and in infected, treated chickens, respectively. The worm burden in infected, treated chickens was 20.4% lower than in infected, untreated chickens.
    Parasitology Research 07/2004; 93(3):235-41. DOI:10.1007/s00436-004-1118-4 · 2.33 Impact Factor
  • M Gabrashanska · S E Teodorova · M Galvez-Morros · M Mitov
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    ABSTRACT: The biogenic elements zinc, manganese and cobalt are essential for metabolic processes in animals. Compounds of nGly.Me2+A. mH2O (Me2+=Zn2+, Mn2+, Co2+; A=Cl(-), SO4(2-), n=1, 2; m=2, 5), as supplements in the diet, were used separately on different experimental groups of male Hisex chickens to correct the mineral deficiency caused by Ascaridia galli infections. An amelioration of body weight gain, reduction of mortality and restoration of trace element levels were estimated in infected chickens. A mathematical model has been proposed for A. galli population kinetics in chickens, taking into account the stimulating effect of these elements on the nematodes. The model parameters are considered as phenomenological constants of the host-parasite system. An agreement with experimental data is observed using, for the parameters psi, alpha, micro and micros, values equal to those calculated in previously investigated A. galli-chicken systems. For parameter nu (immunological constant) the same value was obtained as in a previous experiment with high infection. This model is likely to be suitable for a range of host-nematode systems, including varying degrees of infection and treatment with different trace elements.
    Journal of Helminthology 04/2004; 78(1):25-32. DOI:10.1079/JOH2003204 · 1.30 Impact Factor
  • M Gabrashanska · S E Teodorova · M Mitov
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    ABSTRACT: The effect of dietary cobalt from three different sources on uninfected and Ascaridia galli-infected Hisex chickens, has been studied. The chicken diet was supplemented with 0.06 Co2+ kg-1 food either in the form of two glycine-cobalt compounds or mixed zinc-cobalt basic salt. An excess of dietary cobalt in small doses increases the gain of body weight and decreases host mortality. A greater bioefficiency of cobalt was established in infected chickens. A mathematical model has been used to provide a quantitative interpretation of the observed results. The model solutions of the kinetics of worm numbers and body weight are in a good agreement with experimental data. The model is valid for different degrees of A. galli infections and for treatment with different trace elements. The value of the kinetic parameter, regarded as a phenomenological constant of the host immune response, depends on the degree of infection.
    Journal of Helminthology 01/2003; 76(4):303-10. DOI:10.1079/JOH2002131 · 1.30 Impact Factor
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    Comptes rendus de l'Académie bulgare des sciences: sciences mathématiques et naturelles 01/2003; 56(3):95 - 98. · 0.20 Impact Factor
  • S E Teodorova · M Gabrashanska
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    ABSTRACT: Data from seven experiments with Ascaridia galli-infected chickens have been considered. The results of treatment with neutral and basic copper, zinc and copper-zinc salts and inorganic and organic manganese compounds have been compared. An optimal therapy, containing a pure Cu basic salt (Cu2(OH)3Cl) and an organic Mn compound (2Gly.MnCl2.2H2O), is proposed to correct mineral deficiencies and pathological symptoms and to ensure lower mortality and higher gains in body weight. A mathematical model has been proposed for the growth of a healthy chicken. The relative rates for two growth stages have been determined by the model using data from mean chicken weights. The time course of the average biomass of a single A. galli has been theoretically derived from the same logistic equation describing chicken growth, which in turn might explain, phenomenologically, the mechanisms involved in the biomass growth of eukaryote organisms.
    Journal of Helminthology 04/2002; 76(1):79-85. DOI:10.1079/JOH200189 · 1.30 Impact Factor
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    M. Gabrashanska · S. Tepavitcharova · MM GALVEZ-MORROS · M. Mitov
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    ABSTRACT: The effect of dietary cobalt from three different sources on chicks (uninfected and infected with Ascaridia galli) was studied. Chicks diet was supplemented with 0,06g Co 2+ /kg food either in the form of 2Gly.CoCl 2 .2H 2 O; Gly.CoSO 4 .5H 2 O or (Zn x Co 1-x ) 4 .(OH) 6 .SO 4 .2H 2 O for 20 days. Chicks were divided into eight groups: group 0, control (uninfected and untreated); group 1, untreated and infected with Ascaridia galli; group 2, uninfected and treated with 2Gly.CoCl 2 .2H 2 O; group 3, infected and treated with 2Gly.CoCl 2 .2H 2 O; group 4, uninfected and treated with Gly.CoSO 4 .5H 2 O; group 5, infected and treated with Gly.CoSO 4 .5H 2 O; group 6, uninfected and treated with (Zn x Co 1-x ) 4 .(OH) 6 .SO 4 .2H 2 O and group 7, infected and treated with (Zn x Co 1-x ) 4 .(OH) 6 .SO 4 .2H 2 O. Body weight gain, mortality, worm burden and tissue cobalt contents were investigated. Excess dietary co- balt in small doses increased weight gain and cobalt level and decreased mortality. Worm burden was uneffected. A greater bioefficacy of cobalt in infected chicks was established. Three cobalt compounds in a similar extent affected the performance of chicks.
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    ABSTRACT: The effect of dietary manganese from two different sources on chicks (uninfected and infected with Ascaridia galli) was studied. Chick diet was supplemented with 0.9 g Mn2+ kg-1 food either in the form of MnSO4.H2O or 2Gly. MnCl2.2H2O for 20 days. Chicks were divided into six groups: group 0, control; group 1, control + MnSO4.H2O; group 2, control + 2Gly.MnCl2.2H2O; group 3, infected with A. galli; group 4, infected with A. galli + MnSO4.H2O; and group 5, infected with A. galli + 2Gly.MnCl2.2H2O. Body weight, mortality, worn burden, and liver manganese content were investigated. Excess dietary manganese increased weights and manganese level, but mortality and worm burden were unaffected. A greater bioavailability of manganese from 2Gly.MnCl2.2H2O was established.
    Journal of Helminthology 01/2000; 73(4):313-6. DOI:10.1017/S0022149X99000517 · 1.30 Impact Factor