Cristiane Franco Pinto

University of Campinas, Campinas, Estado de Sao Paulo, Brazil

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Publications (6)3.53 Total impact

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    ABSTRACT: This study evaluated the effects of curing modes and storage conditions on fluoride release of resin cements. In phase 1, the cumulative fluoride release rate from samples of the resin cements (Panavia F 2.0, RelyX Unicem, MaxCem, and BisCem) was quantified after 15 days storage in water (n=4). In phase 2, the fluoride release profiles from the same materials were analyzed during pH cycling (n=4). In this second phase, fluoride was measured at specific times (one, two, three, five, eight, and 15 days). Disk-shaped specimens were prepared (10 mm × 0.5 mm), and the materials were either light activated or allowed to autopolymerize. For both phases, the fluoride release was measured using a fluoride ion-specific electrode. The fluoride release in water was not affected by the curing mode of RelyX Unicem and Maxcem resin cements. Panavia F. 2.0 and BisCem resin cements, either light cured or autopolymerized modes, released higher amounts of fluoride in water than the other self-adhesive cements. In phase 2, the concentration of fluoride released decreased from the first day of pH cycling until the 15th day for all resin cements, for both curing modes, regardless of the storage solution used (demineralizing/remineralizing). The fluoride release rate during pH cycling by Panavia F 2.0 and MaxCem was not affected by the curing mode. The effect of the curing mode on fluoride ion release in water or during pH cycling was product dependent.
    Operative Dentistry 09/2011; 37(1):63-70. · 1.31 Impact Factor
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    ABSTRACT: Objectives: This study tested the fluoride-release rate and the microtensile bond strength to enamel and dentin of two self-etching dental adhesives. Methods: The buccal bovine enamel and dentin surfaces (n=7) were flattened with SiC paper (#600), bonded with Clearfil Protect Bond/Kuraray Med. (CPB) or One-Up Bond F Plus/Tokuyama Dental (OBP) and restored with Filtek Z350 composite resin (3M ESPE). Restored teeth were sectioned in order to obtain bonded beams, which were submitted to pH-cycling regimen (PHC) or not. The PHC consisted of demineralization (DE - 8h/day) and remineralization (RE - 16h/day) cycles at 37 C during 8 days. The solutions were renewed daily and 2 mL of each solution was collected during DE and RE cycles. The fluoride release analysis were performed using an ion-selective electrode (96-09, Orion). After PHC, the beams were tested in a universal testing machine (4411, Instron) at a crosshead speed of 0.5 mm/min until failure. The microtensile bond strength data were analyzed by two-way ANOVA and Tukey test, while fluoride released analysis were analyzed by Friedman and Wilcoxon tests (p< 0.05). Results: The results did not show statistical differences on bond strength to enamel between the adhesive systems when submitted or not to PHC. The bond strength of CPB to dentin was greater than OBP, with no influence of PHC. The amount of fluoride released was low and with some variations among the groups. Conclusions: The bond strength of adhesives to enamel and dentin was not influenced by the PHC, however, the CPB showed the highest bond strength to dentin. In general, the fluoride-release rate for both adhesives was similar and did not increase as function of time. Fapesp (06-53828-2) and CNPq (303587/2007-5).
    AADR Annual Meeting 2010; 03/2010
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    ABSTRACT: This study compared the ability of adhesive systems to inhibit in vitro caries lesions in enamel under high cariogenic challenge. Bovine enamel blocks with cavity preparations were restored with AP-X resin composite (Kuraray Med) using four adhesives systems: Clearfil SE Bond (Kuraray Med); Clearfil Protect Bond (Kuraray Med); One-Up Bond F (Tokuyama) and Single Bond (3M ESPE). The specimens were submitted to an eight-day pH-cycling regimen. After cariogenic challenge, the enamel was evaluated to detect caries lesions using cross-sectional microhardness, polarized light microscopy and scanning electronic microscopy. Data from cross-sectional microhardness and polarized light microscopy evaluations were statistically analyzed by ANOVA and Tukey's test. The mineral % volume showed no statistical difference among adhesives (p > 0.05); however, polarized light microscopy analysis showed lower caries lesions with Clearfil Protect Bond (p < 0.05). The scanning electron microscopy images showed greater caries lesions and demineralization areas close to restorations for Clearfil SE Bond, One-Up Bond F and Single Bond compared to Clearfil Protect Bond. The pH-cycling regimen promoted subsurface enamel demineralization in all specimens treated. The polarized light microscopy and scanning electronic microscopy analyses showed that Clearfil Protect Bond seems to produce lower enamel demineralization around restorations; however, cross-sectional microhardness did not demonstrate differences among the adhesives.
    Operative Dentistry 01/2010; 35(3):345-52. · 1.31 Impact Factor
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    ABSTRACT: To evaluate the effect of a novel antibacterial and fluoride-releasing adhesive formulation on enamel demineralization under sucrose exposure in situ (simulating high caries risk conditions). This crossover, blind study was performed in two phases of 14 days. Volunteers (n = 14) wore an intraoral appliance containing four bovine enamel blocks with cavity preparations restored using self-etching primers/composite resin (Clearfil SE Bond or Clearfil Protect Bond/Clearfil AP-X). The volunteers dropped 20% sucrose solution 8x/day and used fluoridated dentifrice 3x/day. After 14 days, enamel mineral loss was assessed by cross-sectional microhardness (CSMH), and the demineralization areas in enamel adjacent to the restoration were examined by scanning electron microscopy (SEM) and polarizing light microscopy (PLM). The CSMH data were analyzed using ANOVA and Tukey's test (p < 0.05). Enamel blocks restored with Clearfil Protect Bond showed higher mineral content (%vol) adjacent to restorations than Clearfil SE Bond only for the first site of microhardness measurement and close to enamel surface (20 microm). SEM and PLM analysis suggested that the Clearfil Protect Bond adhesive promoted less enamel demineralization around restorations. After 14 days of cariogenic challenge, the findings suggested that Clearfil Protect Bond might help to control the demineralization around restorations in cases of high caries risk.
    The journal of adhesive dentistry 09/2009; 11(4):293-7. · 0.91 Impact Factor
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    ABSTRACT: This study evaluated the effect of 10% carbamide peroxide (CP) bleaching on Knoop surface microhardness (KHN) and morphology of sound enamel and enamel with early artificial caries lesions (CL) after pH-cycling model (pHcm). Human dental enamel blocks were randomly divided into 6 groups (n=10): 1 - sound enamel bleached (S) with CP (Rembrandt/Den-Mat); 2 - S and submitted to pHcm; 3 - CL bleached with CP; 4 - CL stored in artificial saliva and submitted to pHcm; 5 - CL treated with placebo gel and submitted to pHcm; 6 - CL bleached with CP and submitted to pHcm. Enamel blocks with known initial KHN values were demineralized (groups 3 to 6) and submitted to 12 day pHcm (groups 2, 4, 5 and 6). After demineralization and treatments, KHN was determined and the specimens were examined using scanning electron microscopy (SEM). Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. The results showed that among CL groups (3 to 6) only the group 3 presented remineralization after treatments. S groups (1 and 2) showed higher KHN and presented less formation of porosities on enamel surface than CL groups after treatments. In conclusion, bleaching procedures on enamel with CL did not exacerbate the demineralization, but should be indicated with caution.
    Brazilian dental journal 02/2009; 20(1):48-53.
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    ABSTRACT: The aim of this study was to evaluate the surface roughness, microhardness and morphology of human enamel exposed to six bleaching agents (at baseline and post-treatment). Human dental enamel samples were obtained from human third molars and randomly divided into seven groups (n = 11): control, Whiteness Perfect--10% carbamide peroxide (10% CP), Colgate Platinum--10% CP, Day White 2Z--7.5% hydrogen peroxide (7.5% HP), Whiteness Super--3% CP, Opalescence Quick--35% CP and Whiteness HP--35% HP. Bleaching agents were applied according to manufacturers' instructions. The control group remained not treated and stored in artificial saliva. Microhardness testing was performed with a Knoop indentor and surface roughness was analyzed with a profilometer. Morphologic observations were carried out with scanning electron microscopy (SEM). Results were statistically analyzed by two-way analysis of variance and Tukey's test (5%), and revealed a significant decrease in microhardness values and a significant increase in surface roughness post-bleaching. Changes in enamel morphology after bleaching were observed under SEM. It was concluded that bleaching agents can alter the microhardness, roughness and morphology of dental enamel surface.
    Brazilian Oral Research 01/2004; 18(4):306-11.