Cristiane Franco Pinto

University of Campinas, Conceição de Campinas, São Paulo, Brazil

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Publications (10)9.52 Total impact

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    ABSTRACT: To evaluate the in situ effect of fluoride and MDPB-containing adhesives on antibacterial activity around restorations in conditions of high caries risk. Bovine enamel and dentin blocks were restored with a fluoride-containing (One-up Bond F Plus - OP) or a MDPB and fluoride-containing adhesive (Clearfil Protect Bond - PB). Volunteers (n = 17) wore an intra-oral appliance containing three enamel and three dentin blocks, aligned side-by-side and restored with OP or PB and one enamel and dentin block (controls). The cariogenic challenge was carried out in two phases of 14 days each. The counts of total streptococci (TM), mutans streptococci (MS) and lactobacilli (LB) were analyzed in the biofilm formed. Cross-sectional microhardness (CSM) and polarized light microscopy (PLM) evaluated caries lesions around the restorations and the demineralization extension. Data obtained by CSM testing was analyzed by Split-Split Plot ANOVA (P < 0.05). PLM and microbiota results were analyzed by Wilcoxon test (P < 0.05). TM and MS counts were highest for the OP enamel restorations, and these presented higher lesion depths than PB in both the enamel and dentin. The CSM in dentin was the lowest at 60 μm from the restoration wall. None of the adhesives prevented demineralization and bacteria growth, but PB reduced the amount of oral pathogens in enamel and demineralization around restorations in enamel and dentin.
    American journal of dentistry 06/2015; 28(3):167-73. · 0.85 Impact Factor
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    ABSTRACT: Objectives. The objective of this study was to evaluate the effect of chemical collagen cross-linkers on the bond strength of three self-etching adhesives to dentin. Materials and methods. Exposed dentin from the buccal surface of 45 incisors bovine was used to analyze the effect of chemical cross-linkers. The control groups were tested with self-etching adhesives (Clearfil SE Bond, Clearfil SE Protect and One-up Bond F Plus) according to the manufacturer instructions. Two cross-linkers agents were tested: 5% glutaraldehyde and 6.5% proanthocyanidin-rich grape seed extract (both for 10 min). After surface treatments with the agents, the surfaces were washed with distilled water, followed by the bonding/build-up procedures. Restored teeth were prepared for microtensile bond strength test and specimens tested in a universal testing machine (0.5 mm/min) after 24 h storage. Fracture sites of the bonded interface qualitatively evaluated. Results. According to two-way ANOVA and Tukey test (p<0.05) glutaraldehyde pretreatment did not affect the microtensile bond strength of any of two self-etching systems (p>0.05). However, when the grape seed extract was used with Clearfil SE Bond, the dentin bond strength values increased (p<0.05), but decrease for the One-up Bond F Plus treated-group (p<0.05). For the Clearfil SE Protect there was no difference between treatments (p>0.05). Conclusions. The effect of the application of grape seed extract cross-linker was product-dependent and glutaraldehyde did not affect the bond strength to dentin.
    International Journal of Adhesion and Adhesives 04/2015; 60. DOI:10.1016/j.ijadhadh.2015.04.008 · 1.77 Impact Factor
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    ABSTRACT: Purpose: This study evaluated the effects of pH cycling on fluoride release and bond strength of two self-etching adhesive systems to both enamel and dentin. The ultramorphology of the interfaces produced by the adhesive systems were also analyzed. Materials and methods: The buccal surfaces of bovine incisors were flattened to expose enamel and dentin, which were bonded with either Clearfil Protect Bond (CPB) or One-Up Bond F Plus (OBP). The bonded samples were prepared for microtensile bond strength (μTBS) testing, fluoride ion release, and transmission electron microscopy. pH cycling comprised demineralization (8 h/day) and remineralization (16 h/day) cycles for 8 days. The μTBS data were analyzed by two-way ANOVA, while fluoride release was analyzed using the Friedman and Wilcoxon tests. Results: The adhesives presented similar bond strengths to enamel. However, the dentin bond strength of CPB was higher than that of OBP. pH cycling did not influence enamel or dentin μTBS. The amount of fluoride released from the bonded enamel and dentin was low and varied among the groups. The morphological evaluation showed that the thickness of the dentin hybrid layers was similar for both adhesives. Conclusion: The pH-cycling regime did not affect enamel or dentin bond strengths. In enamel, both the self-etching adhesives tested presented similar bond strengths, but in dentin, Clearfil Protect Bond showed higher dentin bonding than One-Up Bond F Plus.
    The journal of adhesive dentistry 01/2015; 17(1). DOI:10.3290/j.jad.a33343 · 1.31 Impact Factor
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    ABSTRACT: This study evaluated the effects of curing modes and storage conditions on fluoride release of resin cements. In phase 1, the cumulative fluoride release rate from samples of the resin cements (Panavia F 2.0, RelyX Unicem, MaxCem, and BisCem) was quantified after 15 days storage in water (n=4). In phase 2, the fluoride release profiles from the same materials were analyzed during pH cycling (n=4). In this second phase, fluoride was measured at specific times (one, two, three, five, eight, and 15 days). Disk-shaped specimens were prepared (10 mm × 0.5 mm), and the materials were either light activated or allowed to autopolymerize. For both phases, the fluoride release was measured using a fluoride ion-specific electrode. The fluoride release in water was not affected by the curing mode of RelyX Unicem and Maxcem resin cements. Panavia F. 2.0 and BisCem resin cements, either light cured or autopolymerized modes, released higher amounts of fluoride in water than the other self-adhesive cements. In phase 2, the concentration of fluoride released decreased from the first day of pH cycling until the 15th day for all resin cements, for both curing modes, regardless of the storage solution used (demineralizing/remineralizing). The fluoride release rate during pH cycling by Panavia F 2.0 and MaxCem was not affected by the curing mode. The effect of the curing mode on fluoride ion release in water or during pH cycling was product dependent.
    Operative Dentistry 09/2011; 37(1):63-70. DOI:10.2341/10-328-L · 1.67 Impact Factor

  • 08/2011; 13(1/2).
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    ABSTRACT: This study compared the ability of adhesive systems to inhibit in vitro caries lesions in enamel under high cariogenic challenge. Bovine enamel blocks with cavity preparations were restored with AP-X resin composite (Kuraray Med) using four adhesives systems: Clearfil SE Bond (Kuraray Med); Clearfil Protect Bond (Kuraray Med); One-Up Bond F (Tokuyama) and Single Bond (3M ESPE). The specimens were submitted to an eight-day pH-cycling regimen. After cariogenic challenge, the enamel was evaluated to detect caries lesions using cross-sectional microhardness, polarized light microscopy and scanning electronic microscopy. Data from cross-sectional microhardness and polarized light microscopy evaluations were statistically analyzed by ANOVA and Tukey's test. The mineral % volume showed no statistical difference among adhesives (p > 0.05); however, polarized light microscopy analysis showed lower caries lesions with Clearfil Protect Bond (p < 0.05). The scanning electron microscopy images showed greater caries lesions and demineralization areas close to restorations for Clearfil SE Bond, One-Up Bond F and Single Bond compared to Clearfil Protect Bond. The pH-cycling regimen promoted subsurface enamel demineralization in all specimens treated. The polarized light microscopy and scanning electronic microscopy analyses showed that Clearfil Protect Bond seems to produce lower enamel demineralization around restorations; however, cross-sectional microhardness did not demonstrate differences among the adhesives.
    Operative Dentistry 05/2010; 35(3):345-52. DOI:10.2341/09-124-L · 1.67 Impact Factor
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    ABSTRACT: Objectives: This study tested the fluoride-release rate and the microtensile bond strength to enamel and dentin of two self-etching dental adhesives. Methods: The buccal bovine enamel and dentin surfaces (n=7) were flattened with SiC paper (#600), bonded with Clearfil Protect Bond/Kuraray Med. (CPB) or One-Up Bond F Plus/Tokuyama Dental (OBP) and restored with Filtek Z350 composite resin (3M ESPE). Restored teeth were sectioned in order to obtain bonded beams, which were submitted to pH-cycling regimen (PHC) or not. The PHC consisted of demineralization (DE - 8h/day) and remineralization (RE - 16h/day) cycles at 37 C during 8 days. The solutions were renewed daily and 2 mL of each solution was collected during DE and RE cycles. The fluoride release analysis were performed using an ion-selective electrode (96-09, Orion). After PHC, the beams were tested in a universal testing machine (4411, Instron) at a crosshead speed of 0.5 mm/min until failure. The microtensile bond strength data were analyzed by two-way ANOVA and Tukey test, while fluoride released analysis were analyzed by Friedman and Wilcoxon tests (p< 0.05). Results: The results did not show statistical differences on bond strength to enamel between the adhesive systems when submitted or not to PHC. The bond strength of CPB to dentin was greater than OBP, with no influence of PHC. The amount of fluoride released was low and with some variations among the groups. Conclusions: The bond strength of adhesives to enamel and dentin was not influenced by the PHC, however, the CPB showed the highest bond strength to dentin. In general, the fluoride-release rate for both adhesives was similar and did not increase as function of time. Fapesp (06-53828-2) and CNPq (303587/2007-5).
    AADR Annual Meeting 2010; 03/2010
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    ABSTRACT: To evaluate the effect of a novel antibacterial and fluoride-releasing adhesive formulation on enamel demineralization under sucrose exposure in situ (simulating high caries risk conditions). This crossover, blind study was performed in two phases of 14 days. Volunteers (n = 14) wore an intraoral appliance containing four bovine enamel blocks with cavity preparations restored using self-etching primers/composite resin (Clearfil SE Bond or Clearfil Protect Bond/Clearfil AP-X). The volunteers dropped 20% sucrose solution 8x/day and used fluoridated dentifrice 3x/day. After 14 days, enamel mineral loss was assessed by cross-sectional microhardness (CSMH), and the demineralization areas in enamel adjacent to the restoration were examined by scanning electron microscopy (SEM) and polarizing light microscopy (PLM). The CSMH data were analyzed using ANOVA and Tukey's test (p < 0.05). Enamel blocks restored with Clearfil Protect Bond showed higher mineral content (%vol) adjacent to restorations than Clearfil SE Bond only for the first site of microhardness measurement and close to enamel surface (20 microm). SEM and PLM analysis suggested that the Clearfil Protect Bond adhesive promoted less enamel demineralization around restorations. After 14 days of cariogenic challenge, the findings suggested that Clearfil Protect Bond might help to control the demineralization around restorations in cases of high caries risk.
    The journal of adhesive dentistry 09/2009; 11(4):293-7. · 1.31 Impact Factor
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    Cristiane Franco Pinto · Adriana Franco Paes Leme · Vanessa Cavalli · Marcelo Giannini ·
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    ABSTRACT: This study evaluated the effect of 10% carbamide peroxide (CP) bleaching on Knoop surface microhardness (KHN) and morphology of sound enamel and enamel with early artificial caries lesions (CL) after pH-cycling model (pHcm). Human dental enamel blocks were randomly divided into 6 groups (n=10): 1 - sound enamel bleached (S) with CP (Rembrandt/Den-Mat); 2 - S and submitted to pHcm; 3 - CL bleached with CP; 4 - CL stored in artificial saliva and submitted to pHcm; 5 - CL treated with placebo gel and submitted to pHcm; 6 - CL bleached with CP and submitted to pHcm. Enamel blocks with known initial KHN values were demineralized (groups 3 to 6) and submitted to 12 day pHcm (groups 2, 4, 5 and 6). After demineralization and treatments, KHN was determined and the specimens were examined using scanning electron microscopy (SEM). Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. The results showed that among CL groups (3 to 6) only the group 3 presented remineralization after treatments. S groups (1 and 2) showed higher KHN and presented less formation of porosities on enamel surface than CL groups after treatments. In conclusion, bleaching procedures on enamel with CL did not exacerbate the demineralization, but should be indicated with caution.
    Brazilian dental journal 02/2009; 20(1):48-53. DOI:10.1590/S0103-64402009000100008
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    Cristiane Franco Pinto · Rogério de Oliveira · Vanessa Cavalli · Marcelo Giannini ·
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    ABSTRACT: The aim of this study was to evaluate the surface roughness, microhardness and morphology of human enamel exposed to six bleaching agents (at baseline and post-treatment). Human dental enamel samples were obtained from human third molars and randomly divided into seven groups (n = 11): control, Whiteness Perfect--10% carbamide peroxide (10% CP), Colgate Platinum--10% CP, Day White 2Z--7.5% hydrogen peroxide (7.5% HP), Whiteness Super--3% CP, Opalescence Quick--35% CP and Whiteness HP--35% HP. Bleaching agents were applied according to manufacturers' instructions. The control group remained not treated and stored in artificial saliva. Microhardness testing was performed with a Knoop indentor and surface roughness was analyzed with a profilometer. Morphologic observations were carried out with scanning electron microscopy (SEM). Results were statistically analyzed by two-way analysis of variance and Tukey's test (5%), and revealed a significant decrease in microhardness values and a significant increase in surface roughness post-bleaching. Changes in enamel morphology after bleaching were observed under SEM. It was concluded that bleaching agents can alter the microhardness, roughness and morphology of dental enamel surface.
    Brazilian Oral Research 10/2004; 18(4):306-11. DOI:10.1590/S1806-83242004000400006 · 0.94 Impact Factor