Kwon-Ha Yoon

Wonkwang University, Riri, Jeollabuk-do, South Korea

Are you Kwon-Ha Yoon?

Claim your profile

Publications (90)191.69 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: X-ray phase-contrast imaging can provide images with much greater soft-tissue contrast than conventional absorption-based images. In this paper, we describe differential X-ray phase-contrast images of insect specimens that were obtained using a grating-based Talbot interferometer and a laboratory X-ray source with a spot size of a few tens of micrometers. We developed the interferometer on the basis of the wavelength, periods, and height of the gratings; the field of view depends on the size of the grating, considering the refractive index of the specimen. The phase-contrast images were acquired using phase-stepping methods. The phase contrast imaging provided a significantly enhanced soft-tissue contrast compared with the attenuation data. The contour of the sample was clearly visible because the refraction from the edges of the object was strong in the differential phase-contrast image. Our results demonstrate that a grating-based Talbot interferometer with a conventional X-ray tube may be attractive as an X-ray imaging system for generating phase images. X-ray phase imaging obviously has sufficient potential and is expected to soon be a great tool for medical diagnostics.
    Journal- Korean Physical Society 12/2015; 65(12):2111-2116. DOI:10.3938/jkps.65.2111 · 0.42 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals. The authors have developed a new method for administering intraperitoneal injections in mice, with the goal of causing less stress during handling and injection. Here, they compare their novel technique with a conventional technique in three experiments. In the first experiment, the authors administered intraperitoneal injections of contrast medium using either technique and then used micro-computed tomography to evaluate the placement and retention of the medium. In the second and third experiments, the authors administered intraperitoneal injections or control treatments, then sampled blood to determine circulating concentrations of stress-related hormones. Imaging showed that both the novel and the conventional techniques properly delivered a contrast medium into the peritoneal cavity. The novel technique was also associated with lower concentrations of stress-related hormones than was the conventional technique. These results indicate that this novel technique might be beneficial to investigators that use intraperitoneal injections with mice.
    Lab Animal 10/2015; 44(11):440-444. DOI:10.1038/laban.880 · 0.74 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Ingestion of Citrus unshiu Markovich (CUM) has been widely used as an alternative therapy for thousands years in Korean traditional medicine. Both anti-osteoclastic actions of CUM extracts (CUME) and the possibility for development as a healthy food product were investigated. CUME significantly (p<0.05) and dose-dependently diminished the receptor activator of nuclear factor kappa-B ligand (RANKL)-induced formation of tartrate resistant acid phosphatase (TRAP)-positive osteoclasts. At the molecular level, CUME interrupted osteoclastogenesis based on selective stimulation of ERK and Akt activation. CUME caused down-regulation of osteoclast-specific marker gene expression derived from impairment of c-Fos and nuclear factor of activated T cells c1 (NFATc1) activation. Treatment with CUME caused localization failure of filamentous actin (F-actin) rings during cellularization and, subsequently, mature osteoclasts suffered malfunction of osteolytic activity. CUME has potential for prevention of osteoporosis. © 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
    Food science and biotechnology 10/2015; 24(5):1837-1843. DOI:10.1007/s10068-015-0240-7 · 0.65 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Harpagoside (HAR) is a natural compound isolated from Harpagophytum procumbens (devil's claw) that is reported to have anti-inflammatory effects; however, these effects have not been investigated in the context of bone development. The current study describes for the first time that HAR inhibits receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis in vitro and suppresses inflammation-induced bone loss in a mouse model. HAR also inhibited the formation of osteoclasts from mouse bone marrow macrophages (BMMs) in a dose-dependent manner as well as the activity of mature osteoclasts, including filamentous actin (F-actin) ring formation and bone matrix breakdown. This involved a HAR-induced decrease in extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinase (JNK) phosphorylation, leading to the inhibition of Syk-Btk-PLCγ2-Ca(2+) in RANKL-dependent early signaling, as well as the activation of c-Fos and nuclear factor of activated T cells cytoplasmic 1 (NFATc1), which resulted in the down-regulation of various target genes. Consistent with these in vitro results, HAR blocked lipopolysaccharide (LPS)-induced bone loss in an inflammatory osteoporosis model. However, HAR did not prevent ovariectomy-mediated bone erosion in a postmenopausal osteoporosis model. These results suggest that HAR is a valuable agent against inflammation-related bone disorders but not osteoporosis induced by hormonal abnormalities.
    Journal of Natural Products 08/2015; 78(9). DOI:10.1021/acs.jnatprod.5b00233 · 3.80 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Pemetrexed, a multitarget antifolate used to treat malignant mesothelioma and non-small cell lung cancer (NSCLC), has been shown to stimulate autophagy. In this study, we determined whether autophagy could be induced by pemetrexed and simvastatin cotreatment in malignant mesothelioma and NSCLC cells. Furthermore, we determined whether inhibition of autophagy drives apoptosis in malignant mesothelioma and NSCLC cells. Malignant mesothelioma MSTO-211H and A549 NSCLC cells were treated with pemetrexed and simvastatin alone and in combination to evaluate their effect on autophagy and apoptosis. Cotreatment with pemetrexed and simvastatin induced greater caspase-dependent apoptosis and autophagy than either drug alone in malignant mesothelioma and NSCLC cells. 3-Methyladenine (3-MA), ATG5 siRNA, bafilomycin A, and E64D/pepstatin A enhanced the apoptotic potential of pemetrexed and simvastatin, whereas rapamycin and LY294002 attenuated their induction of caspase-dependent apoptosis. Our data indicate that pemetrexed and simvastatin cotreatment augmented apoptosis and autophagy in malignant mesothelioma and NSCLC cells. Inhibition of pemetrexed and simvastatin-induced autophagy was shown to enhance apoptosis, suggesting that this could be a novel therapeutic strategy against malignant mesothelioma and NSCLC.
    Oncotarget 08/2015; 6(30). DOI:10.18632/oncotarget.5022 · 6.36 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate whether suppression of tumor microvasculature by double anti-angiogenic protein (DAAP) treatment could increase the extent of radiofrequency ablation (RFA)-induced coagulation in a murine renal cell carcinoma model. Renal cell carcinoma cell lines were implanted subcutaneously into 10 nude mice. Four mice received adenoviral DAAP treatment and 6 mice received sterile 0.9% saline solution as DAAP-untreated group. The effect of DAAP was evaluated according to the vascularity by contrast-enhanced ultrasound (CEUS) using microbubbles. Four DAAP-treated mice and 4 DAAP-untreated mice were then treated with RFA, resulting in 3 groups: no-therapy (n = 2), RFA only (n = 4), and RFA combined with DAAP treatment (n = 4). Immediately after RFA, the size of coagulation necrosis and mitochondrial enzyme activity were compared between the groups using analysis of variance (ANOVA) and post hoc test. The contrast enhancement ratio for tumor vascularization on CEUS was significantly lower in the DAAP treated group than in DAAP-untreated group (30.2 ± 9.9% vs. 77.4 ± 17.3%; p = 0.021). After RFA, the mean coagulation diameter was 0 mm for no-therapy group, 6.7 ± 0.7 mm for the RFA only group and 8.5 ± 0.4 mm for the RFA with DAAP group (ANOVA, p < 0.001). The area of viable mitochondria within the tumor was 27.9 ± 3.9% in no-therapy group, 10.3 ± 4.5% in the RFA only group, and 2.1 ± 0.7% in the RFA with DAAP group (ANOVA, p < 0.001). Our results suggest the potential value of combining RFA with anti-angiogenic therapy.
    Korean journal of radiology: official journal of the Korean Radiological Society 07/2015; 16(4):776-82. DOI:10.3348/kjr.2015.16.4.776 · 1.57 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: Mobile computed tomography (CT) with a cone-beam source is increasingly used in the clinical field. Mobile cone-beam CT (CBCT) has great merits; however, its clinical utility for brain imaging has been limited due to problems including scan time and image quality. OBJECTIVE: The aim of this study was to develop a dedicated mobile volumetric CBCT for obtaining brain images, and to optimize the imaging protocol using a brain phantom. METHODS: The mobile volumetric CBCT system was evaluated with regards to scan time and image quality, measured as signal-to-noise-ratio (SNR), contrast-to-noise-ratio (CNR), spatial resolution (10% MTF), and effective dose. Brain images were obtained using a CT phantom. RESULTS: The CT scan took 5.14 s at 360 projection views. SNR and CNR were 5.67 and 14.5 at 120 kV/10 mA. SNR and CNR values showed slight improvement as the X-ray voltage and current increased (p < 0.001). Effective dose and 10% MTF were 0.92 mSv and 360 μ m at 120 kV/10 mA. Various intracranial structures were clearly visible in the brain phantom images. CONCLUSIONS: Using this CBCT under optimal imaging acquisition conditions, it is possible to obtain human brain images with low radiation dose, reproducible image quality, and fast scan time.
    Journal of X-Ray Science and Technology 07/2015; 23(4):473-480. DOI:10.3233/XST-150502 · 1.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Adipokines derived from adipocytes are important factors that act as circulating regulators of bone metabolism. C1q/tumor necrosis factor (TNF)-related Protein-3 (CTRP3) is a novel adipokine with multiple effects such as lowering glucose levels, inhibiting gluconeogenesis in the liver, and increasing angiogenesis and anti-inflammation. However, the effects and the mechanisms of CTRP3 on bone metabolism, which is regulated by osteoblasts and osteoclasts, have not been investigated. Here, we found that CTRP3 inhibited osteoclast differentiation induced by osteoclastogenic factors in bone marrow cell-osteoblast co-cultures, but did not affect the ratio of receptor activator of nuclear factor κB (NF-κB) ligand (RANKL) to osteoprotegerin (OPG) induced by osteoclastogenic factors in osteoblasts. We also found that CTRP3 inhibited osteoclast differentiation from mouse bone marrow macrophages (BMMs) induced by RANKL in a dose-dependent manner without cytotoxicity. Functionally, CTRP3 inhibited the F-actin formation and bone resorbing activity of mature osteoclasts. Pretreatment with CTRP3 significantly inhibited RANKL-induced expression of c-Fos and nuclear factor of activated T-cells (NFATc1), essential transcription factors for osteoclast development. Surprisingly, the activation of AMP-activated protein kinase (AMPK) was considerably increased by pretreatment with CTRP3 for 1h. The CTRP3-stimulated AMPK activation was also maintained during RANKL-induced osteoclastogenesis. CTRP3 did not affect RANKL-induced p38, ERK, JNK, Akt, IκB, CREB, and calcium signaling (Btk and PLCγ2). These results suggest that CTRP3 plays an important role as a negative regulator of RANKL-mediated osteoclast differentiation by acting as an inhibitor of NFATc1 activation through the AMPK signaling pathway. Furthermore, CTRP3 treatment reduced RANKL-induced osteoclast formation and bone destruction in mouse calvarial bone in vivo based on micro-CT and histologic analysis. In conclusion, these findings strongly suggest that CTRP3 deserves new evaluation as a potential treatment target in various bone diseases associated with excessive osteoclast differentiation and bone destruction. Copyright © 2015. Published by Elsevier Inc.
    Bone 06/2015; 79. DOI:10.1016/j.bone.2015.06.011 · 3.97 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Prohibitin-1 (PHB) regulates diverse cellular processes by controlling several signaling pathways. In this study, we investigated the functional involvement of PHB in osteoclast differentiation. PHB expression was time-dependently increased by RANKL in BMMs. However, the retroviral over-expression of PHB strongly inhibited the expression of c-Fos and NFATc1, and activation of p38-Elk-1-SRE signaling pathway. Anti-osteoclastogenic action of PHB was significantly inhibited by constitutively active forms of MKK6, but not Elk-1. Collectively, PHB negatively regulates the formation of mature osteoclasts via inhibition of MKK6 activity that affects the activation of the p38-Elk-1 signaling axis required for the expression of c-Fos and NFATc1. Copyright © 2015. Published by Elsevier Inc.
    Biochemical and Biophysical Research Communications 06/2015; 463(4). DOI:10.1016/j.bbrc.2015.06.053 · 2.30 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: Technologies employing digital X-ray devices are developed for mobile settings. OBJECTIVE: To develop a mobile digital X-ray fluoroscopy (MDF) for intraoperative guidance, using a novel flat panel detector to focus on diagnostics in outpatient clinics, operating and emergency rooms. METHODS: An MDF for small-scale field diagnostics was configured using an X-ray source and a novel flat panel detector. The imager enabled frame rates reaching 30 fps in full resolution fluoroscopy with maximal running time of 5 minutes. Signal-to-noise (SNR), contrast-to-noise (CNR), and spatial resolution were analyzed. Stray radiation, exposure radiation dose, and effective absorption dose were measured for patients. RESULTS: The system was suitable for small-scale field diagnostics. SNR and CNR were 62.4 and 72.0. Performance at 10% of MTF was 9.6 lp/mm (53 μm) in the no binned mode. Stray radiation at 100 cm and 150 cm from the source was below 0.2 μGy and 0.1 μGy. Exposure radiation in radiography and fluoroscopy (5 min) was 10.2 μGy and 82.6 mGy. The effective doses during 5-min-long fluoroscopy were 0.26 mSv (wrist), 0.28 mSv (elbow), 0.29 mSv (ankle), and 0.31 mSv (knee). CONCLUSIONS: The proposed MDF is suitable for imaging in operating rooms.
    Journal of X-Ray Science and Technology 06/2015; 23(3):365-372. DOI:10.3233/XST-150495 · 1.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The roots of Ostericum koreanum (OK) Maximowicz have traditionally been used to produce an herbal medicine reported to possess anti-inflammatory, anti-oxidant, antimicrobial, and antitumor activities; however, its effect on bone metabolism has not yet been reported. The present study examined the effects of OK extract on lipopolysaccharide (LPS)-induced bone loss in mice by investigating bone structure and the levels of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) in serum and bone marrow fluid (BMF). The effects of OK extract on osteoclastogenesis were also investigated in mouse bone marrow macrophages by examining the formation of tartrate-resistant acid phosphatase (TRAP)-positive cells, the actin ring, and bone resorption activity. OK reduced LPS-induced bone destruction in vivo via a decrease in the RANKL/OPG ratio. Furthermore, it suppressed the formation of TRAP-positive cells and the actin ring, and reduced the bone-resorbing activity of mature osteoclasts. OK also significantly down-regulated the expression of various osteoclast-specific genes. However, it did not affect osteoblast differentiation, or the expression of genes involved in this process. These results demonstrated that OK prevented LPS-induced bone loss by decreasing the RANKL/OPG ratio in serum and BMF, and inhibited osteoclast differentiation and function, suggesting that OK represents a potential therapeutic drug for the treatment of osteoclast-associated bone diseases.
    The American Journal of Chinese Medicine 05/2015; 43(3):495-512. DOI:10.1142/S0192415X15500317 · 2.76 Impact Factor
  • Young Hwan Lee · Han Ah Lee · Taeyeong Heo · Kwon-Ha Yoon ·

    Ultrasound in Medicine & Biology 04/2015; 41(4):S139. DOI:10.1016/j.ultrasmedbio.2014.12.544 · 2.21 Impact Factor
  • Chong-Ho Lee · Jong Hyun Ryu · Young-Hwan Lee · Kwon-Ha Yoon ·
    [Show abstract] [Hide abstract]
    ABSTRACT: A dedicated extremity cone-beam computed tomography (CBCT) was introduced recently, rapidly becoming an attractive modality for extremity imaging. This study aimed at evaluating the effectiveness of a curtain shaped lead shielding in reducing the exposure of patients to scattered radiation in dedicated extremity CBCT. A dedicated extremity CBCT scanner was used. The lead shielding curtain was 42 cm x 60 cm with 0.5 mm lead equivalent. Scattered radiation dose from CBCT was measured using thermoluminescence dosimetry chips at 20 points, at different distance and direction from CT gantry. Two sets of scattered radiation dose measurements were performed before and after installation of curtain-shaped lead shield, and percentage reduction in dose in air was calculated. Mean radiation exposure dose at measured points was 34.46 ± 48.40 μGy without curtains, and 9.67 ± 4.53 μGy with curtains, exhibiting 71.94% reduction (p = 0.000). The use of lead shielding curtains significantly reduced scattered radiation at 0.5 m, 1.0 m, and 1.5 m from CT gantry, with percent reduction of 84.8%, 58.0% and 35.5%, respectively (p = 0.000, 0.000, 0.002). The percent reduction in the diagonal (+45°, -45°) and vertical forward (0°) directions were 86.3%, 83.1%, and 77.7%, respectively, and were statistically significant (p = 0.029, 0.020, 0.041). Shielding with lead curtains suggests an easy and effective method for reducing patient exposure to radiation in extremity CBCT imaging. Advances in knowledge: Lead shielding curtains are an effective technique to reduce scattered radiation dose in dedicated extremity CBCT, with higher dose reduction closer to the gantry opening.
    The British journal of radiology 03/2015; 88(1050):20140866. DOI:10.1259/bjr.20140866 · 2.03 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This paper describes the medical image information system environment for medical image share. The external interface of this system used a cloud service and we adapted the devices for connections with PACS and smart devices. One problem with this type of approach is the accumulated network latency that can arise from such a deployment. For this reason, we suggested a medical image information system environment using data synchronization methods. Our approach is designed as synchronization methods using detection of creation image data on components of system. Also, we used the cloud computing environment, which reduced the number of high-latency image transmissions. Finally, we show the data synchronization process of the system with imaging application services based on a cloud-computing service. Also, we evaluated the response time to compare to mobile PACS. The experimental results show that our system outperforms mobile PACS. As results, our approach is shown to deliver on par or even better results.
    Multimedia Tools and Applications 03/2015; DOI:10.1007/s11042-015-2506-8 · 1.35 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Pemetrexed is a multitargeted antifolate used for the treatment of malignant mesothelioma and non-small cell lung cancer (NSCLC). However, the mechanism by which pemetrexed induces apoptosis remains unclear. In the present study, we investigated the involvement of reactive oxygen species (ROS) and sirtuin 1 (SIRT1) in pemetrexed-induced apoptosis in MSTO-211 malignant mesothelioma cells and A549 NSCLC cells. Pemetrexed enhanced caspase-dependent apoptosis, induced intracellular ROS generation, and downregulated SIRT1 in the MSTO-211 and A549 cells. Pemetrexed-induced apoptosis, which was prevented by pretreatment with N-acetyl-cysteine (NAC), was mediated by effects on the mitochondria, including mitochondrial membrane potential transition (MPT) and cytosolic release of cytochrome c, and also involved regulation of SIRT1 expression. Interference with SIRT1 expression using siRNA enhanced pemetrexed-induced apoptosis through mitochondrial dysfunction and ROS generation, whereas resveratrol, an activator of SIRT1, protected against pemetrexed-induced apoptosis. These results show that pemetrexed induces apoptosis in MSTO-211 mesothelioma cells and A549 NSCLC cells through mitochondrial dysfunction mediated by ROS accumulation and SIRT1 downregulation.
    Oncology Reports 03/2015; 33(5). DOI:10.3892/or.2015.3830 · 2.30 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pleuropulmonary paragonimiasis produces no specific symptoms or radiologic findings, allowing for the possibility of misdiagnosis. We evaluated the specific clinical and pleural fluid features of pleuropulmonary paragonimiasis masquerading as pleural tuberculosis. We retrospectively analyzed the clinical and radiologic characteristics of 20 patients diagnosed with pleuropulmonary paragonimiasis between 2001 and 2011. In total, 17 patients presented with respiratory symptoms, including dyspnea (30%), hemoptysis (20%), cough (20%), and pleuritic chest pain (15%). Chest radiographs revealed intrapulmonary parenchymal lesions, including air-space consolidation (30%), nodular opacities (20%), cystic lesions (15%), ground-glass opacities (10%), and pneumothorax (5%). A pleural f luid examination revealed eosinophilia, low glucose levels, and high lactate dehydrogenase (LDH) levels in 87%, 76%, and 88% of the patients, respectively. These traits helped to distinguish pleuropulmonary paragonimiasis from other pleural diseases such as parapneumonic effusion, malignancy, and pleural tuberculosis. Pleuropulmonary paragonimiasis is often initially misdiagnosed as other pleural diseases. Therefore, it is important to establish the correct diagnosis. In patients with unexplained pleural effusion living in paragonimiasis-endemic areas, pleural fluid obtained by thoracentesis should be examined to distinguish pleuropulmonary paragonimiasis. When marked eosinophilia, high LDH levels, and low glucose levels are identified in pleural fluid, physicians could consider a diagnosis of pleuropulmonary paragonimiasis.
    The Korean Journal of Internal Medicine 01/2015; 30(1):56-61. DOI:10.3904/kjim.2015.30.1.56 · 1.43 Impact Factor
  • Youe Ree Kim · Young Hwan Lee · Kwon-Ha Yoon · Ki Jung Yun ·

    01/2015; 73(5):323. DOI:10.3348/jksr.2015.73.5.323
  • [Show abstract] [Hide abstract]
    ABSTRACT: Homeostatic bone remodeling is vital to maintain healthy bone tissue. Although the receptor activator of nuclear factor κB ligand (RANKL)/RANK axis is considered the master regulator of osteoclastogenesis, the underlying mechanisms including cell fusion remain incompletely defined. Here, we introduce a new axis in the formation of MNCs via RANK signaling: the progranulin (PGRN)/PGRN-induced receptor-like gene during osteoclastogenesis (PIRO) axis. When mouse bone marrow-derived macrophages (MBMMs) were stimulated with PGRN in the presence of RANKL, explosive OC formation was observed. PGRN knockdown experiments suggested that endogenous PGRN is an essential component of the RANKL/RANK axis. Our efforts for identifying genes that are induced by PGRN unveiled a remarkably induced (twenty fold) gene named PIRO. Substantial PGRN and PIRO expression was detected 2 days and 3 days, respectively, suggesting their sequential induction. PIRO was predicted to be a five transmembrane domain-containing receptor-like molecule. The tissue distribution of PGRN and PIRO mRNA expression suggested bone marrow cells are the most suitable niche. Mouse and human PIRO are part of a multigene family. Knockdown experiments suggested that PIRO is a direct target for the formation of MNCs by PGRN. PGRN levels were also substantially higher in ovariectomized (OVX) mice than in sham control (SH) mice. These observations suggest that PGRN and PIRO form a new regulatory axis in osteoclastogenesis that is included in RANK signaling in cell fusion and OC resorption of osteoclastogenesis, which may offer a novel therapeutic modality for osteoporosis and other bone-associated diseases. Copyright © 2014, The American Society for Biochemistry and Molecular Biology.
    Journal of Biological Chemistry 11/2014; 290(4). DOI:10.1074/jbc.M114.608786 · 4.57 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The risk of bone-related diseases increases due to the imbalance between bone resorption and bone formation by osteoclasts and osteoblasts, respectively. The goal in the development of antiosteoporotic treatments is an agent that will improve bone through simultaneous osteoblast stimulation and osteoclast inhibition without undesirable side effects. To achieve this goal, numerous studies have been performed to identify novel approaches using natural oriental herbs to treat bone metabolic diseases. In the present study, we investigated the effect of Chrysanthemum indicum extract (CIE) on the differentiation of osteoclastic and osteoblastic cells. CIE inhibited the formation of TRAP-positive mature osteoclasts and of filamentous-actin rings and disrupted the bone-resorbing activity of mature osteoclasts in a dose-dependent manner. CIE strongly inhibited Akt, GSK3β, and IκB phosphorylation in RANKL-stimulated bone marrow macrophages and did not show any effects on MAP kinases, including p38, ERK, and JNK. Interestingly, CIE also enhanced primary osteoblast differentiation via upregulation of the expression of alkaline phosphatase and the level of extracellular calcium concentrations during the early and terminal stages of differentiation, respectively. Our results revealed that CIE could have a potential therapeutic role in bone-related disorders through its dual effects on osteoclast and osteoblast differentiation.
    Evidence-based Complementary and Alternative Medicine 10/2014; 2014:176049. DOI:10.1155/2014/176049 · 1.88 Impact Factor
  • Source
    Dong-Ho Bang · Youngjun Son · Young Hwan Lee · Kwon-Ha Yoon ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: The aim of our study was to assess the hemodynamic change of liver during the Valsalva maneuver using Doppler ultrasonography. Methods: Thirty healthy men volunteers were enrolled (mean age, 25.5±3.64 years). The diameter, minimal and maximal velocities, and volume flow of intrahepatic inferior vena cava (IVC), middle hepatic vein (MHV), and right main portal vein (RMPV) was measured during both rest and Valsalva maneuver. These changes were compared using paired t-test. Results: The mean diameters (cm) of the intrahepatic IVC at rest and Valsalva maneuver were 1.94±0.40 versus 0.56±0.66 (P<0.001). The mean diameter (cm), minimal velocity (cm/sec), maximal velocity (cm/sec), and volume flow (mL/min) of MHV at rest and Valsalva maneuver were 0.60±0.15 versus 0.38±0.20 (P<0.001), -7.98±5.47 versus 25.74±13.13 (P<0.001), 21.34±6.89 versus 35.12±19.95 (P=0.002), and 106.94±97.65 versus 153.90±151.80 (P=0.014), respectively. Those of RMPV at rest and Valsalva maneuver were 0.78±0.21 versus 0.76±0.20 (P=0.485), 20.21±8.22 versus 18.73±7.43 (P=0.351), 26.79±8.85 versus 24.93±9.91 (P=0.275), and 391.52±265.63 versus 378.43±239.36 (P=0.315), respectively. Conclusion: The blood flow velocity and volume flow of MHV increased significantly during Valsalva maneuver. These findings suggest that hepatic vein might play an important role to maintain venous return to the heart during the maneuver.
    08/2014; 34(1). DOI:10.14366/usg.14029

Publication Stats

973 Citations
191.69 Total Impact Points


  • 2003-2015
    • Wonkwang University
      • • Department of Medicine
      • • Institute for Radiological Imaging Science
      Riri, Jeollabuk-do, South Korea
  • 2002-2015
    • Wonkwang University School of Medicine and Hospital
      Riri, Jeollabuk-do, South Korea
  • 2006
    • Chubu University
      • Department of Mechanical Engineering
      Касугай, Aichi, Japan