Fang Wang

Nanjing University of Technology, Nan-ching, Jiangsu Sheng, China

Are you Fang Wang?

Claim your profile

Publications (23)55.5 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Examination of aquaporin (AQP) membrane channels in extremophile plants may increase our understanding of plant tolerance to high salt, drought, or other conditions. Here, we cloned a tonoplast AQP gene (TsTIP1;2) from the halophyte Thellungiella salsuginea and characterized its biological functions. TsTIP1;2 transcripts accumulate to high levels in several organs, increasing in response to multiple external stimuli. Ectopic overexpression of TsTIP1;2 in Arabidopsis significantly increased plant tolerance to drought, salt and oxidative stresses. TsTIP1;2 had water channel activity when expressed in Xenopus oocytes. TsTIP1;2 was also able to conduct H2O2 molecules into yeast cells in response to oxidative stress. TsTIP1;2 was not permeable to Na(+) in Xenopus oocytes, but it could facilitate the entry of Na(+) ions into plant cell vacuoles by an indirect process under high-salinity condition. Collectively, these data showed that TsTIP1;2 could mediate the conduction of both H2O and H2O2 across membranes, and may act as a multifunctional contributor to survival of T. salsuginea in highly stressful habitats.
    Plant and Cell Physiology 11/2013; · 4.13 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: A series of Au–silica (nanosphere) catalysts were prepared by in situ synthesis; highly dispersed gold nanoparticles (GNPs, 6.4 nm) were obtained, and catalytic tests showed good catalytic activity and epoxidation selectivity. To investigate the origin of the GNPs, the solids were characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and other instruments. Some reaction parameters, such as solvent, oxidant concentration, catalyst concentration and reaction time, have been investigated and reaction conditions are optimized.
    New Journal of Chemistry 02/2013; 37(3):769-774. · 2.97 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na(+) /H(+) antiporter crucial for the bacterium's resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaD were generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na(+) concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, AaNhaD-overexpressing tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na(+) /H(+) antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance.
    Journal of Integrative Plant Biology 05/2012; 54(6):412-21. · 3.75 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: Suaeda salsa is a euhalophytic plant that is tolerant to coastal seawater salinity. In this study, we cloned a cDNA encoding an 8.4 kDa chloroplast outer envelope protein (designated as SsOEP8) from S. salsa and characterized its cellular function. Steady-state transcript levels of SsOEP8 in S. salsa were up-regulated in response to oxidative stress. Consistently, ectopic expression of SsOEP8 conferred enhanced oxidative stress tolerance in transgenic Bright Yellow 2 (BY-2) cells and Arabidopsis, in which H(2) O(2) content was reduced significantly in leaf cells. Further studies revealed that chloroplasts aggregated to the sides of mesophyll cells in transgenic Arabidopsis leaves, and this event was accompanied by inhibited expression of genes encoding proteins for chloroplast movements such as AtCHUP1, a protein involved in actin-based chloroplast positioning and movement. Moreover, organization of actin cytoskeleton was found to be altered in transgenic BY-2 cells. Together, these results suggest that SsOEP8 may play a critical role in oxidative stress tolerance by changing actin cytoskeleton-dependent chloroplast distribution, which may consequently lead to the suppressed production of reactive oxygen species (ROS) in chloroplasts. One significantly novel aspect of this study is the finding that the small chloroplast envelope protein is involved in oxidative stress tolerance.
    Plant Cell and Environment 03/2012; 35(3):588-600. · 5.14 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: Allylic oxidation of cycloolefins was carried out over Ag-VPO catalyst using tert-butyl hydroperoxide (TBHP) as oxidant in argon atmosphere, under our reaction conditions, attack of the activated C–H bonds was preferred instead of the epoxidation of C=C bond and corresponding α, β-enones were obtained as the main products. It is a high yielding, safe and eco-friendly method.
    Catalysis Communications - CATAL COMMUN. 01/2011; 15(1):103-107.
  • [show abstract] [hide abstract]
    ABSTRACT: Carbon nanotube supported gold catalysts prepared by deposition–precipitation with urea (DP urea) were characterized by various techniques. Its catalytic activity was examined for the oxidation of styrene using t-butylhydroperoxide as oxidant. This system showed good epoxide selectivity. The other factors, such as solvent, reaction time, concentrations of oxidant and catalyst, have also been investigated and reaction conditions are optimized. It is a novel highly active/selective and reusable heterogeneous catalyst for styrene epoxidation.
    Catalysis Letters 01/2010; 134(1):51-55. · 2.24 Impact Factor
  • Kun Sun, Shuang Cheng, Fang Wang, Li Sun
    [show abstract] [hide abstract]
    ABSTRACT: Recent studies have shown that the ferric uptake regulator (Fur) of Edwardsiella tarda (Fur(Et)) shares high sequence identity with the Escherichia coli Fur (Fur(Ec)) at the N-terminal DNA-binding region. In the present study, the functional importance of the C-terminal region of Fur(Et) was investigated. It was found that Fur(Et) bearing deletion of the C-terminal 12 residues still possesses most of the repressor activity, whereas Fur(Et) bearing deletions of the C-terminal 16 and more than 16 residues are severely affected in activity. Domain swapping analyses indicated that the chimeric Fur proteins (Et75Ec73 and Et75Vh74) consisting of the N-terminal 1-75 region of Fur(Et) fused to the C-terminal 76-148 region of Fur(Ec) and the C-terminal 76-149 region of the Vibrio harveyi Fur (Fur(Vh)), respectively, are fully active. C92 of Fur(Ec) and C137 of Fur(Vh), which are functionally essential in Fur(Ec) and Fur(Vh), respectively, are also essential in Et75Ec73 and Et75Vh74, respectively. Further study identified an artificial Fur protein, EtMF54, which is composed of the N-terminal 49 residues of Fur(Et) and five artificial residues. Compared to Fur(Et), EtMF54 possesses partial Fur activity that is iron-dependent. These results (i) indicate that there exist certain functional/structural compatibilities among Fur(Et), Fur(Ec), and Fur(Vh) at the C-terminal region; (ii) provide insights to the potential location of the regulatory ion-binding site of Fur(Et).
    The Journal of General and Applied Microbiology 10/2009; 55(5):351-8. · 0.74 Impact Factor
  • Junhua Liu, Fang Wang, Raf Dewil
    [show abstract] [hide abstract]
    ABSTRACT: The present paper reports on the use of CeO2 materials supported palladium chloride catalyst for selectively oxidising organic alcohols into aldehydes. Spherical, microsized rod-shaped and spindle-like CeO2 particles are synthesised and characterised by SEM. The catalysts are prepared by loading palladium chloride onto the CeO2 support matrix. A complete characterization of the catalysts is performed. The activity of catalysts is studied by the selective oxidation of various alcohols. The results show that (1) the catalytic activities prepared by spherical cerium are superior to the catalysts prepared by spindle-like or rod-shaped cerium and (2) the catalyst PdCl2/CeO2 (nanospheres) show good activity, high yield, and good stability.
    Catalysis Letters 07/2009; 130(3-4):448-454. · 2.24 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: A series of silver-doped vanadium phosphorus oxides (Ag-VPO) catalysts were prepared by in situ synthesis, the catalytic tests showed that silver component significantly increased the overall activity for the oxidation of styrene by tert-butyl hydroperoxide (TBHP). It is interesting that Ag-VPO catalysts show no epoxidation selectivity but good selectivity for benzaldehyde in this study. To investigate the origin of the silver effect, the solids were characterized using X-ray diffraction (XRD), Fourier transformed-infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and other instruments, the (VO)2P2O7, β-VOPO4, Ag(V2P2O10) and Ag2(VO2)(PO4) phases were detected in all the Ag-VPO catalysts. This study also confirms that ratios of P/V play a crucial role in the activity and the selectivity of the catalyst, meanwhile, the other factors, such as solvent, oxidant concentration, reaction time, also have been investigated and reaction conditions are optimized.
    Chemical Engineering Journal. 01/2009;
  • [show abstract] [hide abstract]
    ABSTRACT: Ferric uptake regulator (Fur) is a global regulator involved in multiple aspects of bacterial life. The gene encoding the Vibrio harveyi Fur (FurVh) was cloned from a pathogenic V. harveyi strain isolated from diseased fish. FurVh shares 77% overall sequence identity with the Escherichia coli Fur (FurEc) and could complement a mutant of FurEc. Like FurEc, FurVh, possesses two cysteine residues at positions 92 and 95, yet unlike FurEc, in which these cysteine residues constitute part of the metal ion coordination site and hence are vital to the repressor activity, C92 and C95 of FurVh proved to be functionally inessential. Further study identified a Vibrio Fur signature sequence, which is preserved in all the ten Vibrio Fur proteins that have been discovered to date but in none of the non-vibrio Fur proteins. Site-directed and random mutation analyses of the signature residues, the cysteine residues, and seven highly charged amino acid residues indicated that D9, H32, C137, and K138 of FurVh are functionally important but D9, C137, and K138 can be replaced by more than one functional substitutes. Systematic deletion analysis demonstrated that the C-terminal 12 residues of FurVh are functionally inessential. These results (i) indicated that the activation mechanism, or certain aspects of which, of FurVh is possibly different from that of FurEc; and (ii) suggested that it is not very likely that the C-terminal 12 residues play any significant role in the activation or stability of FurVh; and (iii) provided insights into the potential function of the local structure involving C137 and K138.
    The Journal of Microbiology 01/2009; 46(6):670-80. · 1.28 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: A hybrid complex Zr(PO4)(AgHPO4) was prepared by ion-exchange and was determined using elemental analysis, X-ray diffraction (XRD), Fourier transformed-infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and other instruments. Its catalytic activity was examined on the oxidation of styrene using t-butylhydroperoxide (TBHP) as oxidant under argon atmosphere, this system showed good epoxidation selectivity, the other factors, such as solvent, reaction time, concentrations of oxidant and catalyst, also have been investigated and reaction conditions are optimized. It is a novel highly active/selective and reusable heterogeneous catalyst for styrene epoxidation, while this catalytic system showed no epoxidation selectivity but good enol selectivity for cycloolefins with allylic H.
    Catalysis Communications - CATAL COMMUN. 01/2009; 10(6):868-871.
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: Suaeda salsa is a leaf-succulent euhalophytic plant capable of surviving under seawater salinity. Here, we report the isolation and functional analysis of a novel Suaeda gene (designated as SsTypA1) encoding a member of the TypA/BipA GTPase gene family. The steady-state transcript level of SsTypA1 in S. salsa was up-regulated in response to various external stressors. Expression of SsTypA1 was restricted to the epidermal layers of the leaf and stem in S. salsa, and SsTypA1-green fluorescence protein (GFP) fusion proteins were targeted to the chloroplasts of tobacco leaves. Ectopic over-expression of SsTypA1 rendered the transgenic tobacco plants with significantly increased tolerance to oxidative stress, and this was accompanied by a reduction in H(2)O(2) content. Enzymatic and Western blot analyses revealed that the activity and amount of the thylakoid-bound NAD(P)H dehydrogenase (NDH) complex in the chloroplasts of leaf cells were enhanced. Additionally, an in vitro assay demonstrated that SsTypA1 bound to GTP and possessed GTPase activity that was stimulated by the presence of chloroplast 70S ribosomes. Together, these results suggest that SsTypA1 may play a critical role in the development of oxidative stress tolerance, perhaps as a translational regulator of the stress-responsive proteins involved in reactive oxygen species (ROS) suppression in chloroplast.
    Plant Cell and Environment 08/2008; 31(7):982-94. · 5.14 Impact Factor
  • Fang Wang, Shuang Cheng, Kun Sun, Li Sun
    [show abstract] [hide abstract]
    ABSTRACT: The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur (Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
    The Journal of Microbiology 07/2008; 46(3):350-5. · 1.28 Impact Factor
  • Source
    Fang Wang, Junhua Liu, Xianlun Xu
    [show abstract] [hide abstract]
    ABSTRACT: Chemoselective hydrogenation of para-chloronitrobenzene without any dechlorination over Pt/gamma-ZrP catalyst proceeds effectively, which provides a clean and convenient approach to produce useful para-chloroaniline with excellent activity and selectivity.
    Chemical Communications 06/2008; · 6.38 Impact Factor
  • Junhua Liu, Fang Wang, Xianlun Xu
    [show abstract] [hide abstract]
    ABSTRACT: A hybrid complex Zr(PO4)(AgHPO4) was prepared by ion-exchange and its activity was examined on the allylic oxidation of cycloolefins using tert-butylhydroperoxide (TBHP) as oxidant under argon atmosphere. Under these reaction conditions, attack of the activated C–H bond was preferred instead of the epoxidation of C=C bond, yielding enols as the main products, this is a novel highly active/selective and reusable heterogeneous catalyst.
    Catalysis Letters 01/2008; 120(1):106-110. · 2.24 Impact Factor
  • ChemInform 01/2008; 39(13).
  • [show abstract] [hide abstract]
    ABSTRACT: With oxygen that was supplied by hydrogen peroxide as oxidant, secondary and primary aliphatic alcohols, primary allylic alcohols could be efficiently oxidized to aldehydes or ketones in high yields in the presence of Pd (OAc)2−sodium oxalate (SO) under solvent-free conditions, it is a high yielding and operationally simple method. Acetic acid is testified to attend the reaction, the acetate ligands are environed to serve two purposes: as a ligand for the PdII center and as a base to deprotonate the Pd-bound alcohol, but acetic acid has not shown in previous bidentate ligands systems, so a mechanism is proposed on the basis of the mechanisms reported by the pioneers.
    Catalysis Communications - CATAL COMMUN. 01/2008; 9(3):386-390.
  • Advanced Synthesis & Catalysis 10/2007; 349(16):2439 - 2444. · 5.54 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: The vascular tissue of roots performs essential roles in the physical support and transport of water, nutrients, and signaling molecules in higher plants. The molecular mechanisms underlying the function of root vascular tissue are poorly understood. In this study, we analyzed the expression pattern of AtGRP9, a salt stress-responsive gene encoding a glycine-rich protein, and its interacting partner, in Arabidopsis thaliana. Analysis of GUS or GFP expression under the control of the AtGRP9 promoter showed that AtGRP9 was expressed in the vascular tissue of the root; subcellular localization analysis further demonstrated that AtGRP9 proteins were localized in the cell wall and in the cytoplasm. Yeast two-hybrid analysis revealed that AtGRP9 interacted with AtCAD5, a major cinnamyl alcohol dehydrogenase (CAD) involved in lignin biosynthesis, for which tissue-specific distribution was comparable with that of AtGRP9. These results suggest that AtGRP9 may be involved in lignin synthesis in response to salt stress as a result of its interaction with AtCAD5 in A. thaliana.
    Journal of Plant Research 04/2007; 120(2):337-43. · 2.06 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: The halophyte Thellungiella halophila (salt cress) is an ideal model system for studying the molecular mechanisms of salinity tolerance in plants. Herein, we report the identification of a stress-responsive cyclophilin gene (ThCYP1) from T. halophila, using fission yeast as a functional system. The expression of ThCYP1 is highly inducible by salt, abscisic acid (ABA), H(2)O(2) and heat shock. Ectopic overexpression of the ThCYP1 gene enhance the salt tolerance capacity of fission yeast and tobacco (Nicotiana tabacum L.) cv. Bright Yellow 2 (BY-2) cells significantly. ThCYP1 is expressed constitutively in roots, stems, leaves and flowers, with higher expression occurring in the roots and flowers. The ThCYP1 proteins are distributed widely within the cell, but are enriched significantly in the nucleus. The present results suggest that ThCYP1 may participate in response to stresses in the salt cress, perhaps by regulating appropriate folding of certain stress-related proteins, or in the signal transduction processes.
    Plant Cell Reports 03/2007; 26(2):237-45. · 2.51 Impact Factor

Publication Stats

72 Citations
55.50 Total Impact Points

Institutions

  • 2010–2013
    • Nanjing University of Technology
      Nan-ching, Jiangsu Sheng, China
  • 2007–2012
    • Northeast Institute of Geography and Agroecology
      • • Institute of Microbiology
      • • Institute of Oceanology
      Beijing, Beijing Shi, China
  • 2009
    • Nanjing Normal University
      • College of Chemistry and Environmental Science
      Kao-lan-hsien, Gansu Sheng, China
  • 2007–2008
    • Chinese Academy of Sciences
      • • Institute of Oceanology
      • • Institute of Microbiology
      Peping, Beijing, China