Fang Wang

Guangxi University, Yung-ning, Guangxi Zhuangzu Zizhiqu, China

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Publications (278)963.98 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Magnetocaloric effect and critical behavior of Ni2In-type compound FeMn1−xNixGe were investigated in the present work. By partial substitution of Ni for Mn in FeMnGe, the magnetic transition temperature (TC) is tuned in a wide temperature range between 160 and 320 K with x (Ni concentration) varying from 0 to 0.4. A ferromagnet (FM) to paramagnet (PM) magnetic phase transition is observed and the magnetization has a sharp drop around respective phase transition temperature for all the compounds. The maximum magnetic entropy change is found to be 3.9 J/kg K and 4.1 J/kg K for a 5 T field change with x = 0.2 and 0.3, respectively, making them a promising candidate for magnetic refrigeration near room temperature. To investigate the nature of PM–FM phase transition, the critical behavior near TC was analyzed based on the Arrott plots. The critical values obtained with the conventional Arrott-plot procedure are very close to those predicated by the mean-field model. This suggests a long-range exchange interaction and a second order magnetic phase transition in FeMn1−xNixGe.
    Journal of Alloys and Compounds 08/2015; 639. DOI:10.1016/j.jallcom.2015.03.154 · 2.73 Impact Factor
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    ABSTRACT: Effects of magnetic field heat treatment on the structure and magnetic properties of Sm-Co/α-Fe nanocomposite permanent magnetic materials fabricated by high energy ball milling are investigated in the present work. After a magnetic field heat treatment below 700oC on as-milled amorphous Sm-Co/α-Fe samples, the nanocomposite magnets with strong hard and soft magnetic interaction, showing a hysteresis loop of single phase characteristic, are obtained. The coercivity increases with the increase of annealing temperature. The coercivity, remanence and remanence ratio of the Sm-Co/Fe nanocomposite magnets are all enhanced after a heat treatment at a magnetic field as compared with those of nanocomposite magnets heat treated without a magnetic field. X ray diffraction analysis shows that the diffusion between the Sm-Co hard and α-Fe soft phases is suppressed by the magnetic field applied during the heat treatment process, leading to the inhibition of the grain growth of nanocrystal Sm-Co and α-Fe phases, and a finer nanostructure is obtained. Thus, a higher coercivity, remanence and remanence ratio are realized in Sm-Co/α-Fe nanocomposite magnets after the magnetic field heat treatment. Magnetic field heat treatment also makes the direction of c axis of Sm-Co hard grains along the heat treatment magnetic field direction, leading to an enhancement of magnetic anisotropy of the Sm-Co/Fe nanocomposite magnets.
    Journal of Alloys and Compounds 06/2015; 647. DOI:10.1016/j.jallcom.2015.06.152 · 2.73 Impact Factor
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    ABSTRACT: To investigate the angular distributions of UVA, UVB, and effective UV for erythema and vitamin D (vitD) synthesis, the UV spectral irradiances were measured at ten inclined angles (from 0° to 90°) and seven azimuths (from 0° to 180°) at solar elevation angle (SEA) that ranged from 18.8° to 80° in Shanghai (31.22° N, 121.55° E) under clear sky and the albedo of ground was 0.1. The results demonstrated that in the mean azimuths and with the back to the sun, the UVA, UVB, and erythemally and vitD-weighted irradiances increased with the inclined angles and an increase in SEA. When facing toward the sun at 0°-60° inclined angles, the UVA first increased and then decreased with an increase in SEA; at other inclined angles, the UVA increased with SEA. At 0°-40° inclined angles, the UVB and erythemally and vitD-weighted irradiances first increased and then decreased with an increase in SEA, and their maximums were achieved at SEA 68.7°; at other inclined angles, the above three irradiances increased with an increase in SEA. The maximum UVA, UVB, and erythemally and vitD-weighted irradiances were achieved at an 80° inclined angle at SEA 80° (the highest in our measurements); the cumulative exposure of the half day achieved the maximum at a 60° inclined angle, but not on the horizontal. This study provides support for the assessment of human skin sun exposure.
    International Journal of Biometeorology 05/2015; DOI:10.1007/s00484-015-1005-y · 2.10 Impact Factor
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    ABSTRACT: Soybean (Glycine max) is an important crop for oil and protein resources worldwide. The molecular mechanism of abiotic stress response in soybean is largely unclear. We previously identified multiple stress-responsive WRKY genes from soybean. Here, we further characterized the roles of one of these genes, GmWRKY27, in abiotic stress tolerance using transgenic hairy root assay. GmWRKY27 expression was improved by various abiotic stresses. Overexpression and RNAi analysis demonstrate that GmWRKY27 improved salt and drought tolerance in transgenic soybean hairy roots. Measurement of physiological parameters including reactive oxygen species and proline contents also supported this conclusion. GmWRKY27 inhibits expression of a downstream gene GmNAC29 by binding to the W-boxes in its promoter region. The GmNAC29 is a negative effector of stress tolerance as revealed from the performance of transgenic hairy roots under stress. GmWRKY27 interacts with GmMYB174, which also suppresses GmNAC29 expression and enhances drought stress tolerance. The GmWRKY27 and GmMYB174 may have evolved to adapt to each other for binding to the neighbouring cis-elements in GmNAC29 promoter to co-reduce the promoter activity and gene expression. Our study discloses a valuable mechanism in soybean for stress response by two associated transcription factors. Manipulation of these genes should facilitate stress tolerance in soybean and other crops. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    The Plant Journal 05/2015; DOI:10.1111/tpj.12879 · 6.82 Impact Factor
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    ABSTRACT: By switching position of the N and S atom in the thiazole ring which were similar to the previously reported agent 5-(4-ethoxyphenyl)-4-(3',4',5'-trimethoxyphenyl)thiazol-2-amine, a series of 4,5-diarylthiazole derivatives were synthesized using Friedel-Crafts reaction based on chemical modification of Combrestatatin A-4 (CA-4). Their antiproliferative activities were evaluated and identified as new microtubule destabilizing agents. Structure-activity relationship study indicated that compound 8a with 3,4,5-trimethoxyphenyl group at the C-4 position and 4-ethoxyphenyl group at the C-5 position of 2-amino substituted thiazole was of the most potent inhibitory activity in this series. 8a was found to exhibit the IC50 values of 8.4-26.4nM in five human cancer cell lines, with comparable inhibition effects to CA-4. Moreover, 8a showed potency as a tubulin polymerization inhibitor, with colchicine site binding ability and comparable extent of inhibition against the growth of P-glycoprotein over-expressing multidrug resistant cell lines. Mechanism studies revealed that 8a could block the progression of cell cycle in the G2/M phase and result in cellular apoptosis in cancer cells. As a new tubulin destabilizing agent, 8a was also found high antivascular activity as it concentration-dependently reduced the cell migration and disrupted capillary like tube formation of HUVEC cells. Furthermore, 8a significantly suppressed the tumor growth in HCT116 and SK-OV-3 xenograft models with tumor growth inhibitory rate of 55.12% and 72.7%, respectively. Our studies highlighted that 8a was a promising microtubule targeting antitumor agent. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Bioorganic & medicinal chemistry 04/2015; 23(13). DOI:10.1016/j.bmc.2015.04.055 · 2.95 Impact Factor
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    ABSTRACT: Amino acid prodrugs are known to be very useful for improving the aqueous solubility of sparingly water soluble drugs (Drug Discovery Today2013, 18, 93). Therefore, we synthesized eleven novel combretastatin A-4 amino acid derivatives and evaluated their anti-tumor activities in vitro and in vivo. Among them, compound 15 (valine attached to compound 3, which was shown to be a potent tubulin polymerization inhibitor in our previous study) exhibited high efficacy in tumor-bearing mice, and pharmacokinetic analysis in rats indicated that compound 15 was an effective prodrug as well. Besides, compound 15 significantly inhibited tubulin polymerization in vitro and in vivo by binding to the colchicine binding site. In addition, compound 15 induced cell cycle arrest in the G2/M phase and triggered apoptosis in a caspase-dependent manner. In conclusion, our study showed that compound 15 could have significant anti-tumor activity as a novel microtubule polymerization disrupting agent with improved aqueous solubility. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Bioorganic & medicinal chemistry letters 04/2015; 25(11). DOI:10.1016/j.bmcl.2015.04.028 · 2.33 Impact Factor
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    ABSTRACT: Rare-earth permanent magnetic nanoflakes and nanoparticles have drawn great attention due to their potential applications in fabricating permanent magnets with high performance. In this work, the anisotropic PrCo5 nanoflakes were fabricated via surfactant-assisted ball milling at low temperature and room temperature, and the morphology, microstructure, and magnetic properties were investigated systematically. It is found that, compared with the sample milled at room temperature, the PrCo5 nanoflakes prepared at low temperature have a better crystallinity, stronger [001] out-of-plane texture, more regular shape with smaller thickness, and excellent magnetic properties, especially a higher degree of grain alignment and larger remanence ratio (M r/M s).
    Journal of Applied Physics 04/2015; 117(17):17D142. DOI:10.1063/1.4918342 · 2.19 Impact Factor
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    ABSTRACT: Increasing evidences suggest that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning city, Guangxi, China. In addition, their dietary was monitored using a semi-quantitative dietary questionary (FFQ 23). The results revealed the abundances of Roseburia and Escherichia were significantly greater, whereas Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella and Akkermansia were significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified thirty-three and thirty-four operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae and Lachnospiraceae and the former two were increased in the centenarians; Diet-related OTUs were classified as Bacteroidales, Lachnospiraceae and Ruminococcaceae. The former two were deceased, whereas the later one was increased in the high-fiber diet. The age and high-fiber diet are concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit health benefit of the centenarians.
    Journal of Microbiology and Biotechnology 04/2015; · 1.32 Impact Factor
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    ABSTRACT: Palygorskite (PAL) supported Pd nanoparticle (NP) catalyst was prepared by a simple ion-exchange method. This catalyst showed good catalytic performance for the oxidation of styrene epoxidation (95.2% conversion and 90.2% epoxidation selectivity, TON as high as 13,448 could be achieved). This catalyst was characterized by TEM, XRD, FT-IR and XPS, the data reveal the presence of strong interaction between Pd NP and PAL supports, and the good catalytic performance is closely related to this interaction and the surface polarity of PAL. Meanwhile, the ion-exchanged Pd NPs can be recycled without loss of catalytic efficiency. Ultimately, some reaction parameters have been investigated and reaction conditions are optimized.
    Applied Clay Science 03/2015; 105. DOI:10.1016/j.clay.2014.12.007 · 2.70 Impact Factor
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    ABSTRACT: Centenarians constitute a significant subpopulation in the Bama County of Guangxi province in China. The beneficial effects of intestinal microbiota, especially bifidobacteria of centenarians, have been widely accepted; however, knowledge about Bifidobacterium species in centenarians is not adequate. The aim of this study was to investigate the quantity and prevalence of fecal Bifidobacterium in healthy longevous individuals. Fecal samples from eight centenarians from Bama (aged 100 to 108 years), eight younger elderlies from Bama (aged 80 to 99 years), and eight younger elderlies from Nanning (aged 80 to 99 years) were analyzed using denaturing gradient gel electrophoresis, species-specific clone library, and quantitative polymerase chain reaction technology (qPCR). A total of eight different Bifidobacterium species were detected. B. dentium, B. longum, B. thermophilum, B. pseudocatenulatum/B. catenulatum, and B. adolescentis were common in fecal of centenarians and young elderly. B. minimum, B. saecularmay/B. pullorum/B. gallinarum, and B. mongoliense were found in centenarians but were absent in the younger elderlies. In addition, Bifidobacterium species found in centenarians were different from those found in Bama young elderly and Nanning young elderly, and the principal differences were the significant increase in the population of B. longum (P < 0.05) and B. dentium (P < 0.05) and the reduction in the frequency of B. adolescentis (P < 0.05), respectively. Centenarians tend to have more complex fecal Bifidobacterium species than young elderlies from different regions.
    Current Microbiology 03/2015; 71(1). DOI:10.1007/s00284-015-0804-z · 1.36 Impact Factor
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    ABSTRACT: In our study, transcriptome microarrays were used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We found that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and moderate/poor differentiation. We show that miR-145 targets the DNA damage repair-associated gene Helicase-like transcription factor (HLTF), which is involved in radio-resistance. Moreover, miR-145 over-expression in cervical cancer cells enhances radiosensitivity in vitro and in vivo. These results indicate that targeting miR-145 may be a novel radiosensitizing strategy for cervical cancer. Copyright © 2015. Published by Elsevier B.V.
    FEBS Letters 02/2015; 589(6). DOI:10.1016/j.febslet.2015.01.037 · 3.34 Impact Factor
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    ABSTRACT: This study aims to explore the associations between parental occupational exposures to endocrine disruptors (EDs) and simple isolated congenital heart defects (CHDs). A case-control study with standardized data collection involving 761 children with isolated CHDs and 609 children without any congenital malformations was conducted in Sichuan Province of China from March in 2012 to August in 2013. An adjusted job exposure matrix was used for occupational EDs exposure assessment. Logistic regression analysis was performed to assess the associations between parental occupational EDs exposures and CHDs. Maternal age at births, maternal education level, gravity, parity, induced abortion, folic acid use, medication use, drinking capacity and area of residence periconceptionally were selected as confounding factors for mothers. For fathers, we selected the following confounding factors: paternal education level, smoking, drinking frequencies and drinking capacity periconceptionally. Maternal occupational exposures to phthalates are associated with perimembranous ventricular septal defect (PmVSD) (P = 0.001, adjusted OR 3.7, 95 % CI 1.7-8.0), patent ductus arteriosus (PDA) (P = 0.002, adjusted OR 3.8, 95 % CI 1.6-8.9), secundum atrial septal defect (s-ASD) (P = 0.008, adjusted OR 3.5, 95 % CI 1.4-8.7) and pulmonary valve stenosis (PS) (P = 0.035, adjusted OR 4.2, 95 % CI 1.1-16.0), to alkylphenolic compounds and PmVSD (P = 0.003, adjusted OR 2.2, 95 % CI 1.3-3.6), PDA (P = 0.005, adjusted OR 2.0, 95 % CI 1.1-3.5) and PS (P = 0.004, adjusted OR 3.8, 95 % CI 1.5-9.4), to heavy metals with PmVSD (P = 0.003, adjusted OR 7.3, 95 % CI 2.0-27.6) and s-ASD (P = 0.034, adjusted OR 6.5, 95 % CI 1.1-36.7). Paternal occupational exposures to phthalates are associated with PmVSD (P = 0.035, adjusted OR 1.6, 95 % CI 1.0-2.4) and PS (P = 0.026, adjusted OR 2.4, 95 % CI 1.1-5.2), to alkylphenolic compounds (P = 0.027, adjusted OR 1.5, 95 % CI 1.0-2.2) with PmVSD. In conclusion, parental occupational exposures to some specific EDs, in particular phthalates and alkylphenolic compounds, are associated with an increased risk of some CHD phenotypes. However, the findings need to be considered more circumspectly regarding a crude measure of exposure probabilities and small numbers.
    Pediatric Cardiology 01/2015; 36(5). DOI:10.1007/s00246-015-1116-6 · 1.55 Impact Factor
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    ABSTRACT: Monitoring the element concentrations in the human body is of critical importance for health and longevity. In order to explore the formation mechanism of longevity from the perspective of the body's element loads, this study investigated the prominent feature of element profiles in healthy people over 80 years from Bama County (China), a famous longevous region (LR). The element profiles in nails of elderly people from the LR and a non-longevous region were determined by inductively coupled plasma mass spectrometry, using orthogonal projections to latent structures discriminant analysis (OPLS-DA) for pattern recognition. As a result, four characteristic elements closely related to the healthy elderly people from LR, including Cr, Fe, Mn, and Co, were identified. The concentrations of Cr, Fe, Mn, and Co were significantly increased in the LR group (p < 0.05). The values of fold change of Cr, Fe, Mn, and Co were 3.00, 2.46, 2.24, and 2.21, respectively. These characteristic elements could provide an important material guarantee for health and longevity of elderly people in the LR. The further correlation analysis revealed significant positive correlations between the Sr (r = 0.886), Mn (r = 0.873), Ni (r = 0.786), and Co (r = 0.738) concentrations in nails of elderly people and those in drinking water. Furthermore, significant positive correlations were found between the Se (r = 0.940), Mn (r = 0.833), and Fe (r = 0.733) concentrations in nails and their dietary intake. Consequently, the observations suggested that diet could provide extraordinary reference information in terms of reflecting the feature of element profiles in healthy elderly people over 80 years from the LR.
    Biological Trace Element Research 01/2015; 165(1). DOI:10.1007/s12011-015-0233-7 · 1.61 Impact Factor
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    ABSTRACT: A series of amino acid derivatives of Millepachine were designed, synthesized and evaluated for their solubility and anti-proliferation ability against tumor. The glycine derivative compound 7a exhibited the best potency and possessed long-term inhibitory capability on cell viability. It was also confirmed that 7a could arrest the cell cycle at G2/M phase and trigger apoptosis. Furthermore, indirect immunofluorescence staining revealed anti-tubulin property of 7a, which is consistent of the previously reported derivatives of Millepachine. In vivo, 7a suppressed tumor growth in an MDA-MB-231 xenograft tumor model. In summary, the exploit of 7a was a successful approach directed by the concept of generating amino acid prodrugs with increased bioavailability.This article is protected by copyright. All rights reserved.
    Chemical Biology &amp Drug Design 01/2015; DOI:10.1111/cbdd.12507 · 2.51 Impact Factor
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    ABSTRACT: Magnetocaloric effect and critical behavior of amorphous ribbons Gd54Ho6Co25Al14Si were investigated in the present work. Samples were fabricated by a single roller melt spinning method at a spinning rate of 20m/s and 60m/s, respectively. The Curie temperature is 120K for both samples. A broader phase transition temperature range, however, was found for the sample fabricated at 60m/s. It was attributed to its higher local disordered structure due to its faster quenching speed. The peak of the magnetic entropy change S M PK --- is 11.01J.kg-1.K-1 and its full width of the half maximum is about 90K at a field change of 0-5T, which results in a large refrigerant capacity (about 990J.kg-1). The critical behavior around the phase transition temperature was analyzed based on the Arrott plots and the critical values β, γ and n were obtained with the conventional Arrott-plot procedure. The values of β and γ are very close to those predicated by the mean-field model. The n value, however, obtained from the n M PK ---S --- H curve, is higher than the mean field predicted one, which may be attributed to its amorphous structure.
    IEEE Transactions on Magnetics 01/2015; DOI:10.1109/TMAG.2015.2437367 · 1.21 Impact Factor
  • Zhonghua er ke za zhi. Chinese journal of pediatrics 01/2015; 53(1):76-7.
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    ABSTRACT: To analyze the relationship between oral active vitamin D treatment and mortality in maintenance hemodialysis (MHD) patients. We examined the association of oral calcitriol treatment with mortality in 156 MHD patients (80 men and 76 women; mean age: 59 ± 15 years). The survival analysis of all-cause and cardiovascular mortality was performed using the Kaplan-Meier survival and Cox proportional-hazards analyses. In all, 108 of the 156 patients received active vitamin D treatment. The intact parathyroid hormone level was obviously lower in the patients who received active vitamin D treatment than in those who did not. Throughout the whole follow-up, overall mortality was 16.7% (26 deaths, 13 in each group). The cardiovascular mortality rates were 14.6% (8/48) in the control group and 4.6% (5/108) in the calcitriol group. The crude analysis of all-cause and cardiovascular mortality using the Kaplan-Meier curve showed a significant reduction in mortality risk for patients who received oral active vitamin D compared with those who did not receive it (p = 0.015 and 0.026, respectively). Cox's regression analysis showed that active vitamin D treatment was associated with a significantly lower risk of all-cause mortality (RR = 0.399, 95% CI 0.185-0.862, p = 0.019) and cardiovascular mortality (RR = 0.295, 95% CI 0.094-0.93, p = 0.037). However, after adjusting for potential confounding variables, oral active vitamin D therapy was no longer clearly associated with a lower risk of either all-cause or cardiovascular mortality. Oral active vitamin D treatment was associated with improved survival in MHD patients. However, this survival benefit was smaller than previously reported, and a large cohort study should be performed.
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    ABSTRACT: To analyze the clinical audiological characteristics in Chinese Alport syndrome, and investigate the relationship between the genotypes of Alport syndrome and hearing phenotype. The clinical hearing data of 92 cases diagnosed as Alport syndrome from 2008 August to 2013 August were reviewed and analyzed. All coding exons of COL4A3 and COL4A5 genes were PCR-amplified and sequenced from genomic DNA, or mRNA of COL4A5 gene was RT-PCR-amplified and sequenced from skin fibroblast in 17 cases. Eighty-seven out of 92 cases were found with X-linked dominant inheritance (XLAS); 5 cases with autosomal recessive (ARAS); 44 cases had normal hearing, but 14 young cases had abnormal OAE; 48 cases (52.2%, 35 male, 13 female) had sensorineural hearing loss. A total of 44 cases with XLAS had hearing loss (49.4%), wherein the incidence of hearing impairment was 55.0% in male XLAS, and 37.0% in female XLAS. Mild and moderate hearing loss were found in XLAS. Audiometric curves including groove type (21 cases), descending type (13 cases), flat type (10 cases), high frequency drop type (3 cases) and ascending type (1 case) were found in AS. Sixteen mutations of COL4A3, COL4A5 gene were found in 17 cases with Alport syndrome, including severe mutation in 8 cases with moderate hearing impairment. Mild and moderate hearing impairment, and groove type of audiometric curve are mainly found in Chinese Alport syndrome, which is different from Alport syndrome in western countries. OAE in the early diagnosis of hearing loss is important. Hearing phenotype is related certainly with genotype.
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    ABSTRACT: To analyze the relationship between hyperuricemia and primary nephrotic syndrome in childhood. A retrospective study was carried out in 107 children with primary nephrotic syndrome. The clinical data were analyzed with statistical methods to identify the related factors with hyperuricemia. The morbidity of hyperuricemia in children with primary nephrotic syndrome was 45% (48/107). Compared to those in normal serum uric acid group, the incidence of hypertension (33%, 16/48), serum triglyceride [2.59(1.62-3.87) mmol/L], creatinine [43.85(33.38-56.38)mmol/L], urea [6.11(3.77-8.40)mmol/L] and blood uric acid/creatinine ratio [9.30(7.03-12.72)] increased while creatinine clearance rate [141.74(103.57-160.97)ml/(min·1.73 (2))] decreased in hyperuricemia group. Hyperuricemia in children with primary nephrotic syndrome correlated with the increase of serum creatinine, urea and blood uric acid/creatinine ratio, the decrease of creatinine clearance rate and the occurance of hypertension.
    Zhonghua er ke za zhi. Chinese journal of pediatrics 11/2014; 52(11):859-62.
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    ABSTRACT: SKLB-M8, a derivative of millepachine, showed significant anti-proliferative effects in melanoma cell lines. In this study, we investigated the anti-melanoma and anti-angiogenic activity of SKLB-M8 on three melanoma cell lines (A2058, CHL-1, and B16F10) and human umbilical vein endothelial cells (HUVECs). In vitro, SKLB-M8 showed anti-proliferative activity with IC50 values of 0.07, 0.25, and 0.88 μM in A2058, CHL-1, and B16F10 cell lines, respectively. Flow cytometory analysis showed that SKLB-M8 induced G2/M arrest in three melanoma cell lines, and western blotting demonstrated that SKLB-M8 down-regulated the expression of cdc2, up-regulated p53 in A2058 and CHL-1 cells, and triggered cell apoptosis through down-regulating AKT and phosphorylated mTOR (p-mTOR). SKLB-M8 also inhibited HUVEC proliferation, migration, invasion, and tube formation in vitro with the inhibition of phosphorylated ERK1/2 (p-ERK1/2). In vivo, alginate-encapsulated tumor cell assay revealed that SKLB-M8 suppressed B16F10 tumor angiogenesis. In CHL-1- and B16F10-tumor-bearing mouse models, SKLB-M8 inhibited tumor growth by oral treatment with less toxicity. CD31 immunofluoresence staining and caspase-3 immunohistochemistry indicated that SKLB-M8 inhibited melanoma tumor growth by targeting angiogenesis and inducing caspase3-dependent apoptosis. SKLB-M8 might be a potential anti-melanoma drug candidate.
    Journal of Pharmacological Sciences 10/2014; 126(3). DOI:10.1254/jphs.14077FP · 2.11 Impact Factor

Publication Stats

3k Citations
963.98 Total Impact Points

Institutions

  • 2013–2015
    • Guangxi University
      Yung-ning, Guangxi Zhuangzu Zizhiqu, China
    • Shanghai Jiao Tong University
      • School of Public Health
      Shanghai, Shanghai Shi, China
    • University of Texas MD Anderson Cancer Center
      • Department of Pathology
      Houston, Texas, United States
  • 2012–2015
    • Ningbo University
      Ning-po, Zhejiang Sheng, China
    • Government of the People's Republic of China
      Peping, Beijing, China
  • 2011–2015
    • Sichuan University
      • • State Key Laboratory of Biotherapy
      • • Division of Pediatric Cardiology
      • • State Key Laboratory of Oral Diseases
      Hua-yang, Sichuan, China
    • Langston University
      Langston, Oklahoma, United States
  • 2010–2015
    • Nanjing University of Technology
      Nan-ching, Jiangsu Sheng, China
  • 2005–2015
    • Second Military Medical University, Shanghai
      Shanghai, Shanghai Shi, China
  • 2014
    • Ningxia Medical University
      Ning-hsia, Ningxia Huizu Zizhiqu, China
    • Beijing General Research Institute of Mining and Metallurgy
      Peping, Beijing, China
  • 2013–2014
    • China Medical University (PRC)
      • Department of Environmental Health
      Feng-t’ien, Liaoning, China
  • 2011–2014
    • China Agriculture University-East
      Peping, Beijing, China
  • 2010–2014
    • Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
      Hua-yang, Sichuan, China
  • 2008–2014
    • China Agricultural University
      • • College of Food Science and Nutritional Engineering
      • • College of Agronomy and Biotechnology
      Peping, Beijing, China
  • 2003–2014
    • Peking University
      • • Institute of Mental Health
      • • State Key Laboratory of Natural and Biomimetic Drugs
      Peping, Beijing, China
  • 2012–2013
    • Temple University
      • Department of Physiology
      Philadelphia, Pennsylvania, United States
    • Southeast University (China)
      Nan-ching-hsü, Jiangxi Sheng, China
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
  • 2005–2013
    • Beijing Medical University
      • Department of Pediatrics
      Peping, Beijing, China
  • 2010–2012
    • Tianjin University of Science and Technology
      T’ien-ching-shih, Tianjin Shi, China
  • 2008–2012
    • Jilin University
      • • State Key Lab of Superhard Materials
      • • State Key Laboratory on Integrated Optoelectronics
      Yung-chi, Jilin Sheng, China
  • 2002–2012
    • Chinese Academy of Sciences
      • • Institute of Microbiology
      • • Institute of Physics
      • • Institute of Oceanology
      • • State Key Laboratory of Magnetism
      • • Condensed Matter Physics
      Peping, Beijing, China
  • 2007–2010
    • Technical Institute of Physics and Chemistry
      • Key Laboratory of Photochemical Conversion and Optoelectronic Materials
      Peping, Beijing, China
  • 2009
    • Chinese Academy of Fishery Sciences
      北江, Zhejiang Sheng, China
  • 2006–2009
    • Peking University Health Science Center
      Peping, Beijing, China
  • 2004–2007
    • Hebei University of Technology
      • School of Materials Science and Engineering
      T’ien-ching-shih, Tianjin Shi, China