Bhavesh H Patel

L. M. College of Pharmacy, Ahmedabad, Amadavad, Gujarāt, India

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Publications (8)6.06 Total impact

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    ABSTRACT: This paper describes a validated high-performance thin-layer chromatography (HPTLC) method for simultaneous estimation of rabeprazole (RA) and domperidone (DO) in pure powder and in capsule formulations. An HPTLC method separation is achieved on an aluminum sheet of silica gel 60F(254) using ethyl acetate-methanol-benzene-acetonitrile (30:20:30:20 v/v) as mobile phase. Quantitation is achieved with UV detection at 287 nm over a concentration range of 400-1200 ng/spot and 600-1800 ng/spot with mean recovery of 99.82 +/- 0.74 and 99.43 +/- 0.68 for RA and DO, respectively, in the HPTLC method. This method is simple, precise, and sensitive, and it is applicable for the simultaneous determination of RA and DO in pure powder and in capsule formulation.
    Journal of chromatographic science 05/2008; 46(4):304-7. · 0.79 Impact Factor
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    ABSTRACT: Simple, sensitive high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) methods are developed for the quantitative estimation of rabeprazole and mosapride in their combined pharmaceutical dosage forms. In HPLC, rabeprazole and mosapride are chromatographed using 0.01M 6.5 pH ammonium acetate buffer-methanol-acetonitrile (40:20:40, v/v, pH 5.70+/-0.02) as the mobile phase at a flow rate of 1.0 mL/min. In TLC, the mobile phase is ethyl acetate-methanol-benzene (2:0.5:2.5, v/v). Both the drugs are scanned at 276 nm. The retention times of rabeprazole and mosapride are found to be 4.93+/-0.01 and 9.79+/-0.02, respectively. The Rf values of rabeprazole and mosapride are found to be 0.42+/-0.02 and 0.61+/-0.02, respectively. The linearities of rabeprazole and mosapride are in the range of 400-2000 ng/mL and 300-1500 ng/mL, respectively, for HPLC; in TLC, the linearities of rabeprazole and mosapride are in the range of 400-1200 ng/spot and 300-900 ng/spot, respectively. The limit of detection is found to be 97.7 ng/mL for rabeprazole and 97.6 ng/mL for mosapride in HPLC; in TLC the limit of detection is found to be 132.29 ng/spot for rabeprazole and 98.25 ng/spot for mosapride. The proposed methods can be applied to the determination of rabeprazole and mosapride in combined pharmaceutical products.
    Journal of chromatographic science 02/2008; 46(1):10-4. · 0.79 Impact Factor
  • Jpc-journal of Planar Chromatography-modern Tlc - JPC-J PLANAR CHROMAT-MOD TLC. 01/2008; 21(4):267-270.
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    ABSTRACT: Two simple and accurate methods for the determination of pitavastatin calcium (PIT) in tablet dosage forms were developed and validated using column liquid chromatography (LC) and UV spectrophotometry. The LC separation was achieved on a Phenomenex Luna C18 column (250 mm, 4.6 mm id, 5 microm) in the isocratic mode using acetonitrile-water-triethylamine (80 + 19.8 + 0.2, v/v/v), adjusted to pH 3.5 +/- 0.05 with orthophosphoric acid, as the mobile phase at a flow rate of 1.5 mL/min. The retention time was 5.70 min. Both the methods were performed at 238 nm, the wavelength of maximum absorbance of PIT in methanol. In the LC method, quantification was achieved with a photodiode array detector over the concentration range of 0.1-2.5 microg/mL with a mean recovery of 100.26 +/- 0.75%. In the UV method, quantification was achieved over the concentration range of 2-20 microg/mL with mean recovery of 99.65 +/- 1.24%. Both methods were validated, and the results were compared statistically. They were found to be simple, specific, accurate, and precise. The methods were successfully applied for the determination of PIT in tablet dosage form without any interference from common excipients.
    Journal of AOAC International 01/2008; 92(1):158-64. · 1.23 Impact Factor
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    ABSTRACT: A simple and sensitive reversed phase high performance liquid chromatographic (RP‐HPLC) method has been developed for the quantitative estimation of rabeprazole and domperidone in their combined dosage forms. Rabeprazole and domperidone were chromatographed using 0.01 M, pH 6.5 ammonium acetate buffer:methanol:acetonitrile (40:30:30 v/v, pH 7.44) as the mobile phase at a flow rate of 1.0 mL min at ambient temperature and detected at 287 nm. The retention time (RT) of rabeprazole and domperidone were found to be 6.13±0.01 and 8.38±0.02, respectively. The linearities of rabeprazole and domperidone were in the range of 200–2000 ng/mL and 300–3000 ng/mL, respectively. The limit of detection was found to be 65.67 ng/mL for rabeprazole and 98.33 ng/mL for domperidone. The proposed method was applied for the determination of rabeprazole and domperidone in combined dosage forms.
    Journal of Liquid Chromatography & Related Technologies - J LIQ CHROMATOGR RELAT TECHNO. 01/2007; 30(3):439-445.
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    ABSTRACT: This paper describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous estimation of pantoprazole (PANT) and domperidone (DOM) in pure powder and capsule formulations. The HPLC separation was achieved on a Phenomenex C18 column (250 mm id, 4.6 mm, 5 pm) using 0.01 M, 6.5 pH ammonium acetate buffer-methanol-acetonitrile (30 + 40 + 30, v/v/v, pH 7.20) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using ethyl acetate-methanol (60 + 40, v/v) as the mobile phase. Quantification was achieved with ultraviolet (UV) detection at 287 nm over the concentration range 400-4000 and 300-3000 ng/mL with mean recovery of 99.35+/-0.80 and 99.08+/-0.57% for PANT and DOM, respectively (HPLC method). Quantification was achieved with UV detection at 287 nm over the concentration range 80-240 and 60-180 ng/spot with mean recovery of 98.40+/-0.67 and 98.75+/-0.71% for PANT and DOM, respectively (HPTLC method). These methods are simple, precise, and sensitive, and they are applicable for the simultaneous determination of PANT and DOM in pure powder and capsule formulations.
    Journal of AOAC International 01/2007; 90(1):142-6. · 1.23 Impact Factor
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    ABSTRACT: A rapid, simple, and sensitive HPLC and a densitometric HPTLC method for the determination of omeprazole and domperidone in capsule formulations were developed and validated. For HPLC, the separation of components was achieved on a Phenomenex Rp‐C18 column. Isocratic elution with a mobile phase consisting of 0.01 M pH 6.5, ammonium acetate buffer: methanol:acetonitrile (40:30:30 v/v, pH 7.44±0.02), at a flow rate 1.0 mL/min was employed. Rabeprazole was used as the internal standard. In densitometric HPTLC, separation was achieved on aluminum sheets of silica gel 60F254 using ethyl acetate:methanol:benzene (40:20:40 v/v) as mobile phase. Linear concentration range of HPLC and HPTLC methods were between 400–2000 ng/mL and 120–360 ng/spot for both the drugs, respectively. In HPLC, the detection limit was 131.27 ng/mL for omeprazole and 131.20 ng/mL for domperidone, the mean analytical recovery in determination of omeprazole and domperidone capsules was 99.14±1.81 for omeprazole and 99.63±1.68 for domperidone. Whereas, the detection limit was 40.83 ng/spot for omeprazole and 40.53 ng/spot for domperidone, the mean analytical recovery in determination of omeprazole and domperidone capsules was 99.51±0.91 and 99.48±1.15, respectively, in HPTLC. The components were detected by UV detection at 295 nm. Thus, the proposed method is applicable for routine determination of omeprazole and domperidone in pharmaceutical formulations.
    Journal of Liquid Chromatography &amp Related Technologies 01/2007; 30(12):1749-1762. · 0.57 Impact Factor
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    ABSTRACT: A simple, sensitive, precise and accurate reversed phase liquid chromatographic method has been developed for the simultaneous estimation of atorvastatin (AT) calcium, ramipril (RA) and aspirin (AS) from capsule dosage form. The method was developed using a Phenomenex Luna C18 (250mm, 4.6mm i.d., 5µm) column with a mobile phase consisting of 0.1%, orthophosphoric acid buffer:acetonitrile:methanol (45:50:5v/v/v), pH 3.3, at a flow rate of 1mLmin−1. Detection was carried out with ultra-violet detection at 210nm. The retention times were about 12.19, 2.35, and 3.95min for AT calcium, RA and AS, respectively. The developed method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. The linearity ranges were 1–6µgmL−1 for AT calcium, 0.5–3µgmL−1 for RA and 7.5–45µgmL−1 for AS with mean recoveries of 100.59±0.68, 100.62±0.83 and 100.49±0.73% for AT calcium, RA and AS, respectively. Limit of detection obtained were 29.85ngmL−1 for AT calcium, 4.71ngmL−1 for RA and 85.13ngmL−1 for AS. Impurity of salicylic acid was found in capsule dosage form at the retention time of about 4.84min. The proposed method can be used for the estimation of these drugs in combined dosage forms.
    Chromatographia 69(1):91-95. · 1.44 Impact Factor