Luiz Antonio Carlos Bertollo

Universidade Federal de São Carlos, São Carlos do Pinhal, São Paulo, Brazil

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Publications (130)195.64 Total impact

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    ABSTRACT: tAcanthurus is a representative and widespread genus of marine fish that plays a key role in ecologicaldynamics of coral reefs. Three species are common along coastal reefs of Western Atlantic: A. coeruleus,A. bahianus, and A. chirurgus. The cytogenetic patterns of these species indicated sequential steps ofchromosomal rearrangements dating back 19–5 millions of years ago (M.a.) that accounted for their inter-specific differences. A. coeruleus (2n = 48; 2sm + 4st + 42a), A. bahianus (2n = 36; 12m + 2sm + 4st + 18a), andA. chirurgus (2n = 34; 12m + 2sm + 4st + 16a) share an older set of three chromosomal pairs that were orig-inated through pericentric inversions. A set of six large metacentric pairs formed by Robertsonian (Rb)translocations found in A. bahianus and A. chirurgus and a putative in tandem fusion found in A. chirurgusare more recent events. In the present study, new cytogenetic data are being reported for these threeAcanthurus species based on mapping of repetitive sequences such as ribosomal 18S and 5S rDNA andtelomeric repeats to improve their karyotype evolutionary analyses. The lack of interstitial telomericsequences (ITS) in spite of several centric fusions in A. bahianus and A. chirurgus might be related to thelong period of time after their occurrence (estimated in 5 M.a.). Furthermore, the homeologies amongthe chromosome pairs bearing ribosomal genes, in addition to other structural features, highlight largeconserved chromosomal regions in the three species. Our findings indicate that macrostructural changesoccurred during the cladogenesis of these species were not followed by conspicuous microstructuralrearrangements in the karyotypes.
    Cytogenetic and Genome Research 12/2014; 254:62-66. · 1.84 Impact Factor
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    ABSTRACT: Several chromosomal features of Gerreidae fish have been found to be conserved. In this group, it is unclear whether the high degree of chromosomal stasis is maintained when analyzing more dynamic regions of chromosomes, such as rDNA sites that generally show a higher level of variability. Thus, cytogenetic analyses were performed on 3 Atlantic species of the genus Eucinostomus using conventional banding (C-banding, Ag-NOR), AT- and GC-specific fluorochromes, and fluorescence in situ hybridization mapping of telomeric sequences and 5S and 18S rDNA sites. The results showed that although the karyotypical macrostructure of these species is similar (2n = 48 chromosomes, simple Ag-NORs seemingly located on homeologous chromosomes and centromeric heterochromatin pattern), there are differences in the positions of rDNA subunits 5S and 18S. Thus, the ribosomal sites have demonstrated to be effective cytotaxonomic markers in Eucinostomus, presenting a different evolutionary dynamics in relation to other chromosomal regions and allowing access to important evolutionary changes in this group.
    Genetics and molecular research: GMR 11/2014; 13(4):9951-9959. · 0.99 Impact Factor
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    Uedson Jacobina, Luiz Antonio Carlos Bertollo, Marcelo de Bello Cioffi, Wagner Molina
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    ABSTRACT: n Carangidae, Caranx is taxonomically controversial because of slight morphological differences among species, as well as because of its relationship with the genus Carangoides. Cytogenetic data has contributed to taxonomic and phylogenetic classification for some groups of fish. In this study, we examined the chromosomes of Caranx latus, Caranx lugubris, and Carangoides bartholomaei using classical methods, including conventional staining, C-banding, silver staining for nuclear organizer regions, base-specific fluorochrome, and 18S and 5S ribosomal sequence mapping using in situ hybridization. These 3 species showed chromosome numbers of 2n = 48, simple nuclear organizer regions (pair 1), and mainly centromeric heterochomatin. However, C. latus (NF = 50) and C. bartholomaei (NF = 50) showed a structurally conserved karyotype compared with C. lugubris (NF = 54), with a larger number of 2-armed chromosomes. The richness of GC-positive heterochromatic segments and sites in 5S rDNA in specific locations compared to the other 2 species reinforce the higher evolutionary dynamism in C. lugubris. Cytogenetic aspects shared between C. latus and C. bartholomaei confirm the remarkable phylogenetic proximity between these genera.
    Genetics and molecular research: GMR 11/2014; 13(4):9628 - 9635. · 0.99 Impact Factor
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    ABSTRACT: Mechanisms of accumulation based on typical centromeric drive or of chromosomes carrying pericentric inversions are adjusted to the general karyotype differentiation in the principal Actinopterygii orders. Here, we show that meiotic drive in fish is also supported by preferential establishment of sex chromosome systems and B chromosomes in orders with predominantly bi-brachial chromosomes. The mosaic of trends acting at an infra-familiar level in fish could be explained as the interaction of the directional process of meiotic drive as background, modulated on a smaller scale by adaptive factors or specific karyotypic properties of each group, as proposed for the orthoselection model.
    Anais da Academia Brasileira de Ciências 11/2014; · 0.85 Impact Factor
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    ABSTRACT: Rachycentron canadum , the only representative of the family Rachycentridae, has been the focus of biotechnological interest due to its significant potential in marine fish farming. The chromosome set of this species has been widely investigated with respect to the location of genes and multigene families. A FISH analysis was performed using 4 multigene families as probes, represented by 5S and 18S ribosomal genes and histones H2B-H2A and H3. Earlier data suggested that differential replication of heterochromatin could be partially associated with functional genes. Indeed, our results showed that the DNA contained in heterochromatic regions of R. canadum contains 5S and 18S ribosomal genes as well as the gene sequences of histones H2B-H2A and H3, which were colocalized. The distribution of H3 sequences in all heterochromatic regions, except in 13q, could indicate an important evolutionary role for this class of repetitive sequences. Besides, the presence of chromosome regions bearing multifunctional repetitive sequences formed by H2B-H2A/H3/18S rDNA and H2B-H2A/H3/5S rDNA clusterswas demonstrated for the first time in fishes. The implications of differential histone gene extension and its functionality in the karyotype of R. canadum remain unknown.
    Cytogenetic and Genome Research 10/2014; 2014:1-6. · 1.84 Impact Factor
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    ABSTRACT: In this study, genetic differentiation between karyomorphs A (2n = 42) and D (2n = 39/40) of the wolf fish Hoplias malabaricus, which is comprised of several cryptic species that present a wide variety of diploid chromosome numbers and sex chromosome systems, resulting in the identification of seven distinct karyomorphs (A–G), was investigated using a combination of molecular and cytogenetic tools. Deep sequence divergences for both karyomorphs were observed and indicate a long period of reproductive isolation between karyomorphs A and D. Additionally, one individual with 61 chromosomes was identified, which, as far as is known, is the first case of natural triploidy resulting from the hybridization between these highly differentiated karyomorphs of H. malabaricus. Molecular and cytogenetic analyses revealed that this allotriploid specimen carries two sets of maternal chromosomes from karyomorph D (2n = 40) and one set of chromosomes from karyomorph A (n = 21). Moreover, ribosomal sites and active nucleolus organizer regions from both parental contributors were found in the triploid hybrid. Considering the significant genetic distances between karyomorphs A and D, one of the primary reasons for the lack of recurrent reports of hybridization in the H. malabaricus species complex may be due to post-zygotic barriers, such as hybrid sterility or unviability.
    Journal of Fish Biology 09/2014; · 1.83 Impact Factor
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    ABSTRACT: The process of preferential chromosome segregation during meiosis has been suggested to be responsible for the predominance of certain chromosome types in the karyotypes of mammals, birds and insects. We developed an extensive analysis of the fixation of mono- or bibrachial chromosomes in the karyotypes of the large Actinopterygii fish group, a key link in the evolution of terrestrial vertebrates, in order to investigate the generality of meiotic drive in determining karyotypic macrotrends. Unlike mammals, fishes have markedly undergone several types of preferential chromosomal rearrangements throughout evolution. Data from the analyzed orders indicate a prevalence of karyotypes with few (<33%) or many (>66%) acrocentric chromosomes and a low number of karyotypes with balanced numbers of mono- and bi-brachial elements. Parallel trends towards a higher number of karyotypes with prevalence of monobrachial chromosomes occurred in phylogenetically close orders (e.g. Perciformes and Tetraodontiformes, and in the order Mugiliformes) and in clades with prevalence of bibrachial elements (e.g. Characiformes, Gymnotiformes, Siluriformes, and Cypriniformes). Some orders where fewer species were available for study, such as Atheriniformes and Anguilliformes, showed karyotype assemblages where both trends were present. Our results strongly suggest a primary role of meiotic drive in karyotypic evolution as indicated by the accumulation of monobrachial chromosomes in Perciformes and Cypriniformes, or bibrachial chromosomes in Siluriformes and Characiformes. Further examinations of the interaction between life history traits, environmental characteristics, and the fixation of chromosomal rearrangements would be exceedingly valuable.
    Marine Genomics 05/2014; · 1.34 Impact Factor
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    ABSTRACT: Karyotype analyses of the cryptobenthic marine species Ctenogobius boleosoma and C. smaragdus were performed by means of classical and molecular cytogenetics, including physical mapping of the multigene 18S and 5S rDNA families. C. boleosoma has 2n = 44 chromosomes (2 submetacentrics + 42 acrocentrics; FN = 46) with a single chromosome pair each carrying 18S and 5S ribosomal sites; whereas C. smaragdus has 2n = 48 chromosomes (2 submetacentrics + 46 acrocentrics; FN = 50), also with a single pair bearing 18S rDNA, but an extensive increase in the number of GC-rich 5S rDNA sites in 21 chromosome pairs. The highly divergent karyotypes among Ctenogobius species contrast with observations in several other marine fish groups, demonstrating an accelerated rate of chromosomal evolution mediated by both chromosomal rearrangements and the extensive dispersion of 5S rDNA sequences in the genome. © 2014 S. Karger AG, Basel.
    Cytogenetic and Genome Research 03/2014; · 1.84 Impact Factor
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    ABSTRACT: Isolated oceanic islands constitute interesting model systems for the study of colonization processes, as several climatic and oceanographic phenomena have played an important role in the history of the marine ichthyofauna. The present study describes the presence of two morphotypes of Caranx lugubris, in the St. Peter and St. Paul Archipelago located in the mid-Atlantic. Morphotypes were compared in regard to their morphological and cytogenetic patterns, using C-banding, Ag-NORs, staining with CMA3/DAPI fluorochromes and chromosome mapping by dual-color FISH analysis with 5S rDNA and 18S rDNA probes. We found differences in chromosome patterns and marked divergence in body patterns which suggest that different populations of the Atlantic or other provinces can be found in the Archipelago of St. Peter and St. Paul.
    Helgoland Marine Research 02/2014; 68(1). · 1.44 Impact Factor
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    ABSTRACT: Erythrinus erythrinus, a Neotropical fish species of the Erythrinidae family, has a wide distribution in South America. Previous cytogenetic analysis showed that this species presents extensive karyotype diversity, with 4 karyomorphs (A-D) described herein. This study investigated the karyotypic structure of 2 new populations of E. erythrinus from the Brazilian Pantanal region, in order to improve the knowledge of the chromosomal diversity in this species. Both populations showed typical characteristics of karyomorph A, with 2n = 54 chromosomes (6 m + 2 st + 46 a), without differentiation between males and females. In addition, identical supernumerary B chromosomes, appearing as double minute ones, were also found in both the populations. These findings suggest the presence of mitotic instability in view of their high intra and inter-individual numerical variation. The presence of these chromosomes is likely a basal characteristic for this group, since the same kind of Bs also occurs in some other populations and karyomorphs of E. erythrinus. As such, they are important markers of biodiversity found in this nominal species, which probably corresponds to a species complex.
    Genetics and molecular research: GMR 02/2014; · 0.99 Impact Factor
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    ABSTRACT: In fish cytogenetics the methods of chromosome preparations still need to be improved in order to obtain good metaphase figures for banding technics and more accurate analysis. In this paper we describe a very fast and simples culture method from solid fish tissues. Results of its application to different species as well as about 5-BrdU incorporation, both for chromosome banding and sister chromatid differentiation are reported. The data emphasized the advantage of this short term culture method wich provide excellent chromosome preparations suitables for basic and applied cytogenetic studies.
    Caryologia -Firenze- 01/2014; 44(2):161-166. · 0.63 Impact Factor
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    Roberto Ferreira Artoni, Luiz Antonio Carlos Bertollo
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    ABSTRACT: Some species of Hypostomus from the Upper Paraná Basin (SP) were studied cytogenetically. The diploid chromosome number ranged from 2n= 68 to 2n = 80, with a considerably variable karyotypic structure between species, but no chromosomal differences were observed between males and females. The nucleolar organizer regions (NORs) were also quite variable both intra- and interspecifically in terms of number and size. In general, constitutive heterochromatin was not very abundant and usually located in small blocks. The data suggest that some chromosomal rearrangements, as the robertsonian ones and pericentric inversions, were important for the karyotypic evolution of Hypostomus. These fishes also appear to be a good material for cytotaxonomic studies.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 49(1):81-90.
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    Eliana Feldberg, Luiz Antonio C. Bertollo
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    ABSTRACT: Ten neotropical species of family Cichlidae (Pisces: Perciformes) were analyzed cytogenetically. The results obtained also support the indications that the chromosomal evolution of this group was more conservative than divergent from the numerical point of view. All species showed a chromosome number of 2n = 48, although differences in chromosome morphology were observed. The species Chaetobranchopsis australe exhibited a karyotype consisting of 48 subtelo-acrocentric chromosomes, which is possibly of a more primitive type in relation to the karyotypes of the remaining species investigated. No chromosome differences were detected between males and females, indicating the absence of morphologically differentiated sex chromosomes.
    Caryologia -Firenze- 01/2014; 38(3):257-268. · 0.63 Impact Factor
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    ABSTRACT: Chromosomal data for Myleus micans, a species of the subfamily Serrasalminae endemic to the São Francisco river basin, Brazil, are presented for the first time. The diploid number was found to be 2n=58, with 26M+18SM+8ST+6A chromosomes for both sexes. Heterochromatin was seen in the pericentromeric region in few chromosomes, and in the telomeric region only in the 27th pair. Multiple Ag-NORs were detected after silver nitrate staining, and located in four chromosomes. CMA3 staining also revealed four chromosomes sites, two of them not coincinding with those evidenced by silver nitrate. On the other hand, seven 18S rDNA sites were seen after fluorescent in situ hybridization (FISH), and only one chromosome pair bearing the 5S rDNA genes. The relationships between Myleus and the other Serrasalminae genera are discussed.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 59(2):125-130.
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    E. Pauls, L. A. C. Bertollo
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    ABSTRACT: Both males and females of the fish Prochilodus scrofa have a diploid number of 2n = 54. However, with the exception of a single female, all 59 specimens (25 ♀, 34 ♂) studied had 1–5 minutes accessory chromosomes. The unstable behavior of these chromosomes during mitosis and meiosis as well as their heterochromatic nature, as detected by C-banding, are characteristics that emphasize their supernumerary nature. This is the first report of chromosomes of this type in the Osteichthyes (Pisces).
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 36(4):307-314.
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    Eliana Feldberg, Luiz Antonio C. Bertollo
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    ABSTRACT: In the present study, 10 neotropical species of family Cichlidae (Pisces, Perciformes) were submitted to analysis of the nucleolar organizing regions (NORs). In 8 of these species, the NORs were located in the first pair in the complement and coincided with the secondary constrictions observed there, whereas in Cichlasoma facetum and Geophagus brasiliensis the NORs were located in another chromosome pair. The possibility that the predominant location of NORs in the 1st pair in the karyotype indicates a more primitive condition of this group of fish is discussed. Variations in NOR size between homologous chromosomes of some species were also observed.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 38(3):319-324.
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    P.M. Galetti, L.A.C. Bertollo, O. Moreira-Filho
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    ABSTRACT: Neotropical characiform fishes present two main trends of chromosomal evolution: some taxa show a heterogeneous pattern, with an extensive numerical and structural variation while others show a more homogeneous one, with a clear stability in their karyotype macrostructure. The implications of the populational features and some other conditions to these two general paths are discussed.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 47(3):289-297.
  • Moreira Peres, Adriano Wellington, Luiz Antoniô Carlos Bertollo, Orlando Moreira-Filho
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    ABSTRACT: The family Characidae, one of the largest and more diversified fish families in the Neotropical region, is certainly not a monophyletic group, which is supported by the cytogenetic data showing its extensive chromosome variability. In this work, two species of the genus Serrapinnus, S. heterodon and S. piaba, from the São Francisco river basin were analyzed. Both species presented the same diploid number, 2n=52 chromosomes, and the same karyotypic formula, 16m+20sm+14st+2a. The heterochromatin distribution, evidenced by C-banding, was also similar between both species. On the other hand, two NORs were evidenced in S. heterodon, while a multiple NOR system was characterized in S. piaba, both confirmed by silver nitrate staining as well as by fluorescent in situ hybridization with 18S rDNA probe. Furthermore, two additional karyotypes (cytotypes) were observed in S. heterodon, with 15m+20sm+14st+3a and 17m+20sm+14st+la chromosomes, respectively, characterizing a structural chromosome polymorphism in the population. The differences between the three S. heterodon, karyotypic forms are probably due to pericentric inversions related to the 8th and 26th chromosome pairs.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 60(4):319-324.
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    Orlando Moreira Filho, Luiz Antonio Carlos Bertollo, Pedro Manoel Galetti Junior
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    ABSTRACT: The karyotype of the fish Apereiodon affinis shows 2n = 54 chromosomes for the male and 2n = 55 chromosomes for the female. The data obtained suggest a mechanism of multiple sex chromosomes of the ZZ/ZW1W2 type, where chromosome Z may be number 1 in the complement and chromosomes W1 and W2 are morphologically undifferentiated. This is the first probable occurrence of this type of mechanism to be described for fishes.
    Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics. 01/2014; 33(1):83-91.

Publication Stats

1k Citations
195.64 Total Impact Points

Institutions

  • 1985–2014
    • Universidade Federal de São Carlos
      • • Departamento de Genética e Evolução (DGE)
      • • Department of Physiological Sciences
      São Carlos do Pinhal, São Paulo, Brazil
  • 2013
    • Universidade Federal de São Paulo
      San Paulo, São Paulo, Brazil
  • 2011–2013
    • Universidade Federal do Rio Grande do Norte
      • • Department of Cell Biology and Genetics
      • • Centro de Biociências
      Natal, Estado do Rio Grande do Norte, Brazil
  • 2009
    • Brazilian Institute of Environment and Renewable Natural Resources
      Macapá, Amapá, Brazil
  • 2008
    • Universidade Federal de Viçosa (UFV)
      Viçosa, Minas Gerais, Brazil
    • Universidade Federal de Uberlândia (UFU)
      • Institute of Genetics and Biochemistry (INGEB)
      Uberlândia, Estado de Minas Gerais, Brazil
  • 1999–2008
    • State University of Ponta Grossa
      • • Department of Structural Biology, Molecular and Genetics
      • • Department of General Biology
      Ponta Grossa, Paraná, Brazil
  • 2006
    • Universidade Federal de Mato Grosso do Sul
      • Departamento de Biologia (DBI)
      Campo Grande, Estado de Mato Grosso do Sul, Brazil
    • Fundação Universidade Federal do Rio Grande (FURG)
      São Pedro do Rio Grande do Sul, Rio Grande do Sul, Brazil
  • 2004–2005
    • University of São Paulo
      • • Departamento de Genética e Biologia Evolutiva (IB) (Sao Paulo)
      • • Departamento de Psicologia
      San Paulo, São Paulo, Brazil
  • 1992
    • National University of Misiones
      • Departamento de Biología
      Posadas, Provincia de Misiones, Argentina