Luiz Antonio Carlos Bertollo

Universidade Federal de São Carlos, São Carlos do Pinhal, São Paulo, Brazil

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Publications (74)105.03 Total impact

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    ABSTRACT: Repetitive DNAs comprise the largest fraction of the eukaryotic genome. They include microsatellites or simple sequence repeats (SSRs), which play an important role in the chromosome differentiation among fishes. Rachycentron canadum is the only representative of the family Rachycentridae. This species has been focused on several multidisciplinary studies in view of its important potential for marine fish farming. In the present study, distinct classes of repetitive DNAs, with emphasis on SSRs, were mapped in the chromosomes of this species to improve the knowledge of its genome organization. Microsatellites exhibited a diversified distribution, both dispersed in euchromatin and clustered in the heterochromatin. The multilocus location of SSRs strengthened the heterochromatin heterogeneity in this species, as suggested by some previous studies. The colocalization of SSRs with retrotransposons and transposons pointed to a close evolutionary relationship between these repetitive sequences. A number of heterochromatic regions highlighted a greater complex organization than previously supposed, harboring a diversity of repetitive elements. In this sense, there was also evidence of colocalization of active genetic regions and different classes of repetitive DNAs in a common heterochromatic region, which offers a potential opportunity for further researches regarding the interaction of these distinct fractions in fish genomes.
    Zebrafish 02/2015; DOI:10.1089/zeb.2014.1077 · 2.88 Impact Factor
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    ABSTRACT: With nearly 2,000 species, Gobiidae is the most specious family of the vertebrates. This high level of speciation is accompanied by conspicuous karyotypic modifications, where the role of repetitive sequences remains largely unknown. This study analyzed the karyotype of 2 species of the genus Gobionellus and mapped 18S and 5S ribosomal RNA genes and (CA) 15 microsatellite sequences onto their chromosomes. G. oceanicus (2n = 56; ♂ 12 metacentrics (m) + 4 submetacentrics (sm) + 1 subtelocentric (st) + 39 acrocentrics (a); ♀ 12m + 4sm + 2st + 38a) and G. stomatus (2n = 56; ♂ 20m + 14sm + 1st + 21a; ♀ 20m + 14sm + 2st + 20a) possess the highest diploid chromosome number among the Gobiidae and have different karyotypes. Both species share an XX/XY sex chromosome system with a large subtelocentric X and a small acrocentric Y chromosome which is rich in (CA) 15 sequences and bears 5S rRNA sites. Although coding and noncoding repetitive DNA sequences may be involved in the genesis or differentiation
    Cytogenetic and Genome Research 02/2015; DOI:10.1159/000373909 · 1.84 Impact Factor
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    ABSTRACT: tAcanthurus is a representative and widespread genus of marine fish that plays a key role in ecologicaldynamics of coral reefs. Three species are common along coastal reefs of Western Atlantic: A. coeruleus,A. bahianus, and A. chirurgus. The cytogenetic patterns of these species indicated sequential steps ofchromosomal rearrangements dating back 19–5 millions of years ago (M.a.) that accounted for their inter-specific differences. A. coeruleus (2n = 48; 2sm + 4st + 42a), A. bahianus (2n = 36; 12m + 2sm + 4st + 18a), andA. chirurgus (2n = 34; 12m + 2sm + 4st + 16a) share an older set of three chromosomal pairs that were orig-inated through pericentric inversions. A set of six large metacentric pairs formed by Robertsonian (Rb)translocations found in A. bahianus and A. chirurgus and a putative in tandem fusion found in A. chirurgusare more recent events. In the present study, new cytogenetic data are being reported for these threeAcanthurus species based on mapping of repetitive sequences such as ribosomal 18S and 5S rDNA andtelomeric repeats to improve their karyotype evolutionary analyses. The lack of interstitial telomericsequences (ITS) in spite of several centric fusions in A. bahianus and A. chirurgus might be related to thelong period of time after their occurrence (estimated in 5 M.a.). Furthermore, the homeologies amongthe chromosome pairs bearing ribosomal genes, in addition to other structural features, highlight largeconserved chromosomal regions in the three species. Our findings indicate that macrostructural changesoccurred during the cladogenesis of these species were not followed by conspicuous microstructuralrearrangements in the karyotypes.
    Cytogenetic and Genome Research 12/2014; 254:62-66. DOI:10.1016/j.jcz.2014.11.003 · 1.84 Impact Factor
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    ABSTRACT: Several chromosomal features of Gerreidae fish have been found to be conserved. In this group, it is unclear whether the high degree of chromosomal stasis is maintained when analyzing more dynamic regions of chromosomes, such as rDNA sites that generally show a higher level of variability. Thus, cytogenetic analyses were performed on 3 Atlantic species of the genus Eucinostomus using conventional banding (C-banding, Ag-NOR), AT- and GC-specific fluorochromes, and fluorescence in situ hybridization mapping of telomeric sequences and 5S and 18S rDNA sites. The results showed that although the karyotypical macrostructure of these species is similar (2n = 48 chromosomes, simple Ag-NORs seemingly located on homeologous chromosomes and centromeric heterochromatin pattern), there are differences in the positions of rDNA subunits 5S and 18S. Thus, the ribosomal sites have demonstrated to be effective cytotaxonomic markers in Eucinostomus, presenting a different evolutionary dynamics in relation to other chromosomal regions and allowing access to important evolutionary changes in this group.
    Genetics and molecular research: GMR 11/2014; 13(4):9951-9959. DOI:10.4238/2014.November.27.24 · 0.85 Impact Factor
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    Uedson Jacobina, Luiz Antonio Carlos Bertollo, Marcelo de Bello Cioffi, Wagner Molina
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    ABSTRACT: n Carangidae, Caranx is taxonomically controversial because of slight morphological differences among species, as well as because of its relationship with the genus Carangoides. Cytogenetic data has contributed to taxonomic and phylogenetic classification for some groups of fish. In this study, we examined the chromosomes of Caranx latus, Caranx lugubris, and Carangoides bartholomaei using classical methods, including conventional staining, C-banding, silver staining for nuclear organizer regions, base-specific fluorochrome, and 18S and 5S ribosomal sequence mapping using in situ hybridization. These 3 species showed chromosome numbers of 2n = 48, simple nuclear organizer regions (pair 1), and mainly centromeric heterochomatin. However, C. latus (NF = 50) and C. bartholomaei (NF = 50) showed a structurally conserved karyotype compared with C. lugubris (NF = 54), with a larger number of 2-armed chromosomes. The richness of GC-positive heterochromatic segments and sites in 5S rDNA in specific locations compared to the other 2 species reinforce the higher evolutionary dynamism in C. lugubris. Cytogenetic aspects shared between C. latus and C. bartholomaei confirm the remarkable phylogenetic proximity between these genera.
    Genetics and molecular research: GMR 11/2014; 13(4):9628 - 9635. DOI:10.4238/2014.November.14.7 · 0.85 Impact Factor
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    ABSTRACT: Mechanisms of accumulation based on typical centromeric drive or of chromosomes carrying pericentric inversions are adjusted to the general karyotype differentiation in the principal Actinopterygii orders. Here, we show that meiotic drive in fish is also supported by preferential establishment of sex chromosome systems and B chromosomes in orders with predominantly bi-brachial chromosomes. The mosaic of trends acting at an infra-familiar level in fish could be explained as the interaction of the directional process of meiotic drive as background, modulated on a smaller scale by adaptive factors or specific karyotypic properties of each group, as proposed for the orthoselection model.
    Anais da Academia Brasileira de Ciências 11/2014; DOI:10.1590/0001-3765201420130489 · 0.85 Impact Factor
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    ABSTRACT: Rachycentron canadum , the only representative of the family Rachycentridae, has been the focus of biotechnological interest due to its significant potential in marine fish farming. The chromosome set of this species has been widely investigated with respect to the location of genes and multigene families. A FISH analysis was performed using 4 multigene families as probes, represented by 5S and 18S ribosomal genes and histones H2B-H2A and H3. Earlier data suggested that differential replication of heterochromatin could be partially associated with functional genes. Indeed, our results showed that the DNA contained in heterochromatic regions of R. canadum contains 5S and 18S ribosomal genes as well as the gene sequences of histones H2B-H2A and H3, which were colocalized. The distribution of H3 sequences in all heterochromatic regions, except in 13q, could indicate an important evolutionary role for this class of repetitive sequences. Besides, the presence of chromosome regions bearing multifunctional repetitive sequences formed by H2B-H2A/H3/18S rDNA and H2B-H2A/H3/5S rDNA clusterswas demonstrated for the first time in fishes. The implications of differential histone gene extension and its functionality in the karyotype of R. canadum remain unknown.
    Cytogenetic and Genome Research 10/2014; 2014:1-6. DOI:10.1159/000366301 · 1.84 Impact Factor
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    ABSTRACT: The process of preferential chromosome segregation during meiosis has been suggested to be responsible for the predominance of certain chromosome types in the karyotypes of mammals, birds and insects. We developed an extensive analysis of the fixation of mono- or bibrachial chromosomes in the karyotypes of the large Actinopterygii fish group, a key link in the evolution of terrestrial vertebrates, in order to investigate the generality of meiotic drive in determining karyotypic macrotrends. Unlike mammals, fishes have markedly undergone several types of preferential chromosomal rearrangements throughout evolution. Data from the analyzed orders indicate a prevalence of karyotypes with few (<33%) or many (>66%) acrocentric chromosomes and a low number of karyotypes with balanced numbers of mono- and bi-brachial elements. Parallel trends towards a higher number of karyotypes with prevalence of monobrachial chromosomes occurred in phylogenetically close orders (e.g. Perciformes and Tetraodontiformes, and in the order Mugiliformes) and in clades with prevalence of bibrachial elements (e.g. Characiformes, Gymnotiformes, Siluriformes, and Cypriniformes). Some orders where fewer species were available for study, such as Atheriniformes and Anguilliformes, showed karyotype assemblages where both trends were present. Our results strongly suggest a primary role of meiotic drive in karyotypic evolution as indicated by the accumulation of monobrachial chromosomes in Perciformes and Cypriniformes, or bibrachial chromosomes in Siluriformes and Characiformes. Further examinations of the interaction between life history traits, environmental characteristics, and the fixation of chromosomal rearrangements would be exceedingly valuable.
    Marine Genomics 05/2014; 15. DOI:10.1016/j.margen.2014.05.001 · 1.97 Impact Factor
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    ABSTRACT: Repetitive DNA sequences play an important role in the structural and functional organization of chromosomes, especially in sex chromosome differentiation. The genus Triportheus represents an interesting model for such studies because all of its species analyzed so far contain a ZZ/ZW sex chromosome system. A close relationship has been found between the differentiation of the W chromosome and heterochromatinization, with the involvement of different types of repetitive DNA in this process. This study investigated several aspects of this association in the W chromosome of Triportheus trifurcatus (2n = 52 chromosomes), including the cytogenetic mapping of repetitive DNAs such as telomeric sequences (TTAGGG)n, microsatellites and retrotransposons. A remarkable heterochromatic segment on the W chromosome was observed with a preferential accumulation of (CAC)10, (CAG)10, (CGG)10, (GAA)10 and (TA)15. The retrotransposons Rex1 and Rex3 showed a general distribution pattern in the chromosomes, and Rex6 showed a different distribution on the W chromosome. The telomeric repeat (TTAGGG)n was highly evident in both telomeres of all chromosomes without the occurrence of ITS. Thus, the differentiation of the W chromosome of T. trifurcatus is clearly associated with the formation of heterochromatin and different types of repetitive DNA, suggesting that these elements had a prominent role in this evolutionary process.
    PLoS ONE 03/2014; 9(3):e90946. DOI:10.1371/journal.pone.0090946 · 3.53 Impact Factor
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    ABSTRACT: Isolated oceanic islands constitute interesting model systems for the study of colonization processes, as several climatic and oceanographic phenomena have played an important role in the history of the marine ichthyofauna. The present study describes the presence of two morphotypes of Caranx lugubris, in the St. Peter and St. Paul Archipelago located in the mid-Atlantic. Morphotypes were compared in regard to their morphological and cytogenetic patterns, using C-banding, Ag-NORs, staining with CMA3/DAPI fluorochromes and chromosome mapping by dual-color FISH analysis with 5S rDNA and 18S rDNA probes. We found differences in chromosome patterns and marked divergence in body patterns which suggest that different populations of the Atlantic or other provinces can be found in the Archipelago of St. Peter and St. Paul.
    Helgoland Marine Research 02/2014; 68(1). DOI:10.1007/s10152-013-0365-0 · 1.41 Impact Factor
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    Eliana Feldberg, Luiz Antonio C. Bertollo
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    ABSTRACT: Ten neotropical species of family Cichlidae (Pisces: Perciformes) were analyzed cytogenetically. The results obtained also support the indications that the chromosomal evolution of this group was more conservative than divergent from the numerical point of view. All species showed a chromosome number of 2n = 48, although differences in chromosome morphology were observed. The species Chaetobranchopsis australe exhibited a karyotype consisting of 48 subtelo-acrocentric chromosomes, which is possibly of a more primitive type in relation to the karyotypes of the remaining species investigated. No chromosome differences were detected between males and females, indicating the absence of morphologically differentiated sex chromosomes.
    Caryologia -Firenze- 01/2014; 38(3):257-268. DOI:10.1080/00087114.1985.10797749 · 0.85 Impact Factor
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    Eliana Feldberg, Luiz Antonio C. Bertollo
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    ABSTRACT: In the present study, 10 neotropical species of family Cichlidae (Pisces, Perciformes) were submitted to analysis of the nucleolar organizing regions (NORs). In 8 of these species, the NORs were located in the first pair in the complement and coincided with the secondary constrictions observed there, whereas in Cichlasoma facetum and Geophagus brasiliensis the NORs were located in another chromosome pair. The possibility that the predominant location of NORs in the 1st pair in the karyotype indicates a more primitive condition of this group of fish is discussed. Variations in NOR size between homologous chromosomes of some species were also observed.
    Caryologia -Firenze- 01/2014; 38(3):319-324. DOI:10.1080/00087114.1985.10797755 · 0.85 Impact Factor
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    ABSTRACT: Chromosomal data for Myleus micans, a species of the subfamily Serrasalminae endemic to the São Francisco river basin, Brazil, are presented for the first time. The diploid number was found to be 2n=58, with 26M+18SM+8ST+6A chromosomes for both sexes. Heterochromatin was seen in the pericentromeric region in few chromosomes, and in the telomeric region only in the 27th pair. Multiple Ag-NORs were detected after silver nitrate staining, and located in four chromosomes. CMA3 staining also revealed four chromosomes sites, two of them not coincinding with those evidenced by silver nitrate. On the other hand, seven 18S rDNA sites were seen after fluorescent in situ hybridization (FISH), and only one chromosome pair bearing the 5S rDNA genes. The relationships between Myleus and the other Serrasalminae genera are discussed.
    Caryologia -Firenze- 01/2014; 59(2):125-130. DOI:10.1080/00087114.2006.10797907 · 0.85 Impact Factor
  • Moreira Peres, Adriano Wellington, Luiz Antoniô Carlos Bertollo, Orlando Moreira-Filho
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    ABSTRACT: The family Characidae, one of the largest and more diversified fish families in the Neotropical region, is certainly not a monophyletic group, which is supported by the cytogenetic data showing its extensive chromosome variability. In this work, two species of the genus Serrapinnus, S. heterodon and S. piaba, from the São Francisco river basin were analyzed. Both species presented the same diploid number, 2n=52 chromosomes, and the same karyotypic formula, 16m+20sm+14st+2a. The heterochromatin distribution, evidenced by C-banding, was also similar between both species. On the other hand, two NORs were evidenced in S. heterodon, while a multiple NOR system was characterized in S. piaba, both confirmed by silver nitrate staining as well as by fluorescent in situ hybridization with 18S rDNA probe. Furthermore, two additional karyotypes (cytotypes) were observed in S. heterodon, with 15m+20sm+14st+3a and 17m+20sm+14st+la chromosomes, respectively, characterizing a structural chromosome polymorphism in the population. The differences between the three S. heterodon, karyotypic forms are probably due to pericentric inversions related to the 8th and 26th chromosome pairs.
    Caryologia -Firenze- 01/2014; 60(4):319-324. DOI:10.1080/00087114.2007.10797954 · 0.85 Impact Factor
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    Roberto Ferreira Artoni, Luiz Antonio Carlos Bertollo
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    ABSTRACT: Some species of Hypostomus from the Upper Paraná Basin (SP) were studied cytogenetically. The diploid chromosome number ranged from 2n= 68 to 2n = 80, with a considerably variable karyotypic structure between species, but no chromosomal differences were observed between males and females. The nucleolar organizer regions (NORs) were also quite variable both intra- and interspecifically in terms of number and size. In general, constitutive heterochromatin was not very abundant and usually located in small blocks. The data suggest that some chromosomal rearrangements, as the robertsonian ones and pericentric inversions, were important for the karyotypic evolution of Hypostomus. These fishes also appear to be a good material for cytotaxonomic studies.
    Caryologia -Firenze- 01/2014; 49(1):81-90. DOI:10.1080/00087114.1996.10797353 · 0.85 Impact Factor
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    Orlando Moreira Filho, Luiz Antonio Carlos Bertollo, Pedro Manoel Galetti Junior
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    ABSTRACT: The karyotype of the fish Apereiodon affinis shows 2n = 54 chromosomes for the male and 2n = 55 chromosomes for the female. The data obtained suggest a mechanism of multiple sex chromosomes of the ZZ/ZW1W2 type, where chromosome Z may be number 1 in the complement and chromosomes W1 and W2 are morphologically undifferentiated. This is the first probable occurrence of this type of mechanism to be described for fishes.
    Caryologia -Firenze- 01/2014; 33(1):83-91. DOI:10.1080/00087114.1980.10796821 · 0.85 Impact Factor
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    ABSTRACT: Potamotrygonidae is a representative family of South American freshwater elasmobranchs. Cytogenetic studies were performed in a Potamotrygon species from the middle Negro River, Amazonas, Brazil, here named as Potamotrygon sp. C. Mitotic and meiotic chromosomes were analyzed using conventional staining techniques, C-banding, and detection of the nucleolus organizing regions (NOR) with Silver nitrate (Ag-NOR). The diploid number was distinct between sexes, with males having 2n = 67 chromosomes, karyotype formula 19m + 8sm + 10st + 30a, and fundamental number (FN) = 104, and females having 2n = 68 chromosomes, karyotype formula 20m + 8sm + 10st + 30a, and FN = 106. A large chromosome, corresponding to pair number two in the female karyotype, was missing in the male complement. Male meiotic cells had 33 bivalents plus a large univalent chromosome in metaphase I, and n = 33 and n = 34 chromosomes in metaphase II. These characteristics are consistent with a sex chromosome system of the XX/XO type. Several Ag-NOR sites were identified in both male and female karyotypes. Positive C-banding was located only in the centromeric regions of the chromosomes. This sex chromosome system, which rarely occurs in fish, is now being described for the first time among the freshwater rays of the Amazon basin.
    Genetica 09/2013; DOI:10.1007/s10709-013-9737-2 · 1.75 Impact Factor
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    ABSTRACT: Chromatic polymorphisms in shells may be associated with adaptive characteristics in molluscs. Colouring among Hastula cinerea reveals different patterns in large aggregations of individuals. Although the impacts of temperature are considered critical in the intertidal region, selective effects associated with colouring in H. cinerea shells, in addition to possible physiological consequences, remain an open issue. In this context, H. cinerea is a viable evolutionary model for investigating selective processes related to chromatic polymorphism in shells given its abundance and biological characteristics. Individuals from the same population were classified into three groups according to shell colour: light, reddish and dark. They were then examined with regard to frequency of colour morphs and morphological patterns in each group. Reddish and light-coloured specimens were the most abundant, while those with dark coloration were less common and morphologically distinct from the first two colour morphs. Morphological differences in the anterior region of dark shells, which may be associated with movement ability and lower frequencies in these specimens, suggest possible selective effects on types that are less reflective to light in the intertidal zone.
    Journal of the Marine Biological Association of the UK 06/2013; 93(04). DOI:10.1017/S0025315412001129 · 1.13 Impact Factor
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    Xu Dongdong, Lou Bao, Luiz Antonio Carlos Bertollo, Marcelo de Bello Cioffi
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    ABSTRACT: Despite the theoretical and experimental progress, our understanding on sex chromosome differentiation is still diagrammatic. The accumulation of repetitive DNA sequences is believed to occur in early stages of such differentiation. As fish species present a wide range of sex chromosome systems they are excellent models to examine the differentiation of these chromosomes. In the present study, the chromosomal distribution of 9 mono-, di- and trinucleotide microsatellites were analyzed using fluorescence in situ hybrization (FISH) in rock bream fish (Oplegnathus fasciatus), which is characterized by an X1X2Y sex chromosome system. Generally, the males and females exhibited the same autosomal pattern of distribution for a specific microsatellite probe. The male specific Y chromosome displays a specific amount of distinct microsatellites repeats along both arms. However, the accumulation of these repetitive sequences was not accompanied by a huge heterochromatinization process. The present data provide new insights into the chromosomal constitution of the multiple sex chromosomes and allow further investigations on the true role of the microsatellite repeats in the differentiation process of this sex system.
    Molecular Cytogenetics 03/2013; 6(1):12. DOI:10.1186/1755-8166-6-12 · 2.66 Impact Factor

Publication Stats

631 Citations
105.03 Total Impact Points

Institutions

  • 2001–2015
    • Universidade Federal de São Carlos
      • Departamento de Genética e Evolução (DGE)
      São Carlos do Pinhal, São Paulo, Brazil
  • 2014
    • Universidade Federal do Rio Grande do Norte
      • Department of Cell Biology and Genetics
      Natal, Rio Grande do Norte, Brazil
  • 2011–2013
    • Universidade Federal de São Paulo
      San Paulo, São Paulo, Brazil
  • 2007
    • Museu de História Natural Capão da Imbuia
      Curityba, Paraná, Brazil