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ABSTRACT: A novel bacterial strain designated CJ25(T) was isolated from the estuarine wetland of the Han river in Korea. Identification of this strain was carried out on the basis of polyphasic taxonomy. The isolate was Gram-staining-positive, rod-shaped, non-pigmented and motile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Paenibacillus chondroitinus DSM 5051(T) with 96.1 % similarity. The predominant fatty acids were anteiso-C(15 : 0) (50.25 %), iso-C(16 : 0) (18.54 %) and iso-C(15 : 0) (10.00 %). The major isoprenoid quinone was MK-7. The G+C content of genomic DNA was 50 mol%. According to physiological data and 16S rRNA gene sequence analysis, the isolate was discriminated from related members of the genus Paenibacillus. Therefore, strain CJ25(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus aestuarii sp. nov. is proposed. The type strain is CJ25(T) (=KACC 13125(T) =JCM 15521(T)).
International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 3):644-7. · 2.27 Impact Factor
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ABSTRACT: A yellow-pigmented, rod-shaped, Gram-staining-negative, aerobic bacterial strain, designated CJ7(T), was isolated from a freshwater lake at Chung-Ang University in Anseong, South Korea. Results from 16S rRNA gene sequence analysis revealed that the isolate was phylogenetically affiliated with members of the genus Flavobacterium. Strain CJ7(T) showed sequence similarity values of 91.5-98.0 % to the type strains of recognized Flavobacterium species. The DNA-DNA relatedness between strain CJ7(T) and Flavobacterium hercynium DSM 18292(T), which showed the highest 16S rRNA gene sequence similarity, was 25.4 %. The predominant cellular fatty acids were iso-C(15 : 0) (18.3 %), summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c) (16.1 %), iso-C(17 : 0) 3-OH (8.9 %), iso-C(15 : 0) 3-OH (7.2 %), iso-C(17 : 1)omega9c (6.1 %) and iso-C(15 : 1) (5.9 %). Flexirubin-type pigments were absent. The major isoprenoid quinone was menaquinone-6. The DNA G+C content was 34.5 mol%. The results obtained from this study, with a polyphasic taxonomic approach, suggested that strain CJ7(T) represents a novel species of the genus Flavobacterium for which the name Flavobacterium chungangense sp. nov. is proposed. The type strain is CJ7(T) (=KACC 13353(T)=JCM 15651(T)).
International journal of systematic and evolutionary microbiology 07/2009; 59(Pt 7):1754-8. · 2.27 Impact Factor
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ABSTRACT: A yellow-pigmented, Gram-negative, aerobic bacterial strain comprising rod-shaped cells devoid of flagellar and gliding motility, designated strain JC2129(T), was isolated from tidal flat sediment of Dongmak on Ganghwa Island, South Korea. Results from a 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae; the highest level of nucleotide sequence similarity (91.9%) occurred with Polaribacter dokdonensis DSW-5(T). The predominant cellular fatty acids were iso-C(15:0) (19.8%), iso-C(15:1) G (14.0%), iso-C(17:0) 3-OH (13.7%) and iso-C(13:0) (6.4%). Flexirubin-type pigments were absent. The major isoprenoid quinone was MK-6. The DNA G+C content was 43-45 mol%. Data from a polyphasic taxonomy study suggested that the isolate represents a novel genus and species in the family Flavobacteriaceae, for which the name Actibacter sediminis gen. nov., sp. nov. is proposed. The type strain of Actibacter sediminis is JC2129(T) (=KCTC 12704(T) =JCM 14002(T)).
International journal of systematic and evolutionary microbiology 02/2008; 58(Pt 1):139-43. · 2.27 Impact Factor
