Pengfei Gao

Fourth Military Medical University, Xi’an, Liaoning, China

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Publications (12)6.6 Total impact

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    ABSTRACT: To explore the clinical effectiveness of endoscopic myringoplasty withhomoplastic amniotic membrane. A retrospective study in 43 patients underwent endoscopic myringoplasty with homoplastic amniotic membrane was observed at the wound healing of tympanic membrane perforation and the hearing improve-threshold astudiometry in 43 pati ment after surgery. The tympanic membrane's perforation healing rate was 95.3% (41/43), and air conduction threshold audiometry in language frequency improved average 10 dB. The follow up was 1--4 years, and no recurrence or complications had occurred. The endoscopic myringoplasty could be able to simple myringoplasty whose air conduction threshold audiometry in language frequency was less than 40 dB. It could be used to repair not only the central perforation in tympanic membrane, but also the marginal perforation which has residual tympanic membrane in most of the regional. Human amniotic membrane is an ideal materials for repair of tympanic membrane.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 10/2012; 26(19):882-3.
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    ABSTRACT: To discuss the function and operative method of silicon T tube in laryngotracheal reconstruction. Two hundred and ninety-seven patients of laryngotracheal stenosis were operated with laryngotracheal reconstruction in our department. All of patients were tracheostomy dependent before reconstruction and were placed a silicon T-tube stenting for 3 to 6 months after reconstruction. Two hundred and eighty-nine patients (97.3%) were successfully decannulated with good airway patency and effective phonation. They were followed up from 1 to 10 years, and no recurrence was found. Silicon T-tube is an effective and safe stent for laryngotracheal reconstruction. Paying attention to some application details may avoid the complication and obtain a satisfactory effect.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 10/2011; 25(19):882-3.
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    ABSTRACT: To investigate the effectiveness of various materials in reconstruction of laryngotracheal framework and to analyze the advantages, disadvantage, and indication of each material. The clinical data were retrospectively analyzed, from 337 laryngotracheal stenosis patients undergoing laryngotracheal framework reconstruction with various materials between October 1986 and October 2006. There were 229 males and 108 females aged from 2 to 54 years (median, 23.5 years). According to Cotton's grading criteria for laryngotracheal stenosis, there were 94 cases of grade II, 218 cases of grade III, and 25 cases of grade IV, and all accompanied by laryngotracheal framework defect of 1-5 cm. The costal cartilage autograft was performed in 157 cases, thyroid cartilage graft in 27 cases, nasal septal cartilage graft in 8 cases, sternohyoid myocutaneous rotary door flap graft in 104 cases, hyoid bone flap of sternocleidomastoideus graft in 7 cases, musculo-periosteum flap of sternocleidomastoideus with clavicular periosteum in 21 cases, hydroxyapatite artificial tracheal ring graft in 10 cases, and pedicle myocutaneous flap with "C" shape nickel-titanic alloy net graft in 3 cases. Silastic T-tube was used after reconstruction for 6-12 months. Infection occurred in 5 cases and the incisions healed by second intention, the others achieved healing of incision by first intention. Intratracheal granulation formation occurred in 23 cases and choke when taking food in 6 cases; they were all cured after symptomatic treatment. After operation, 4 cases failed to be followed up and 12 cases did not recover, including 6 cases of costal cartilage autograft, 1 case of hyoid bone flap of sternocleidomastoideus graft, and 5 cases of sternohyoid myocutaneous rotary door flap graft. A total of 321 patients were followed up for 1-10 years (mean, 3.5 years). The patients had no laryngotracheal restenosis with good swallowing function and respiratory function. Different materials of laryngotracheal framework reconstruction have advantages and disadvantage respectively. The appropriate material should be selected according to the special details of pathological change, and the satisfactory curative effect may be obtained.
    Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 05/2011; 25(5):551-3.
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    ABSTRACT: To assess the outcomes of laryngotracheal reconstruction using thyroid alar cartilage grafting in adult patients with laryngotracheal stenosis. Case series with chart review. Tertiary university hospital. Twelve adults who underwent thyroid alar cartilage graft laryngotracheal reconstruction from April 1997 to April 2009 for laryngotracheal stenosis were analyzed. The mean age of the study population was 29 years. Using the Myer-Cotton grading system, 3 patients had grade II stenosis, 7 had grade III, and 2 had grade IV. Seven of the 12 patients had subglottic stenosis, 3 had tracheal stenosis, and 2 had subglottic and upper tracheal stenosis. Nine of 12 (75%) patients were decannulated. Of the patients in whom laryngotracheal reconstruction failed, 2 had grade IV stenosis and 1 had severe grade III stenosis with a long segment of stenotic tissue. The postoperative complications were hematoma of the left laryngeal ventricle at the donor site in 1 patient, granulation tissues in the supraglottic and suprastomal region and at the graft site in 4 patients, and neck wound infection in 1 patient. Ossification of the thyroid alar cartilage was observed in 2 patients. A Ttube remained in situ for 6 to 18 months. Laryngotracheal reconstruction with thyroid alar cartilage graft could be a viable alternative for the treatment of laryngotracheal stenosis in adults. However, it should be used only in cases of limited and minor subglottic or tracheal stenosis.
    Otolaryngology Head and Neck Surgery 02/2011; 144(5):747-50. · 1.73 Impact Factor
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    ABSTRACT: Surgical treatment of a subglottic stenosis can lead to a cricoid cartilage resection, cervical trachea resection, or both that has to be reconstructed with an artificial prosthesis. However, there is at present no standard treatment in the numerous reconstruction procedures, and many prostheses encounter inflammatory rejection and mechanical constraint problems. A porous, metastable β-type titanium alloy prosthesis was developed to improve subglottic rehabilitation. We have designed a porous type of airway prosthesis made of porous metastable β-type titanium alloy. We used this prosthesis (20 mm in length) to reconstruct the cricoid cartilage and the first tracheal ring in 10 mongrel dogs and evaluated its efficacy. One dog died of an accident with anesthesia at 1 week, 1 dog died of pneumonia about 1 month after the operation, and 6 dogs were killed between the third and eighth months after the operation. At death, all the prostheses had become completely incorporated into the host tissue. Endoscopic examination showed no airway obstruction for a postoperative period of 3 to 8 months in 8 dogs. Granulation was observed in 4 cases, and the porous, metastable β-type titanium alloy plate was exposed in 1 case, but these dogs were asymptomatic. Light microscopic and electron microscopic analysis showed a normal mucous membrane growth on the surface of the prosthesis and no visible granulation tissue in the reconstructed cricoid and tracheal cavity. This airway prosthesis provided good results in canine cricotracheal reconstruction, and this study presents the possibility of successful reconstruction of the cricoid and trachea with epithelial regeneration.
    The Journal of thoracic and cardiovascular surgery 12/2010; 141(4):888-94. · 3.41 Impact Factor
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    ABSTRACT: To investigate the effect of the anteroposterior cricoid split on cartilage growth. The rabbits were killed 8 months after the surgery of anteroposterior cricoid split. The larynxes were harvested and the cross-sectional area of the cricoid cartilage were calculated. There was no significant difference in the cross-sectional area of the cricoid cartilage of these three groups. The anteroposterior cricoid split might have no adverse effect on subsequent growth of cricoid cartilage.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 03/2007; 21(3):126-7.
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    ABSTRACT: To investigate the effect of the quarter-section cricoid split on cartilage growth. The rabbits were killed 8 months after surgery of anterior, anteroposterior and quarter section cricoid split. The larynxes were harvested and the cross sectional areas of the cricoid cartilage were calculated. There was no significant in the cross sectional area of the cricoid cartilage of any of the four groups. The quarter section cricoid split has no adverse effect on subsequent growth of cricoid cartilage and animals.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 10/2006; 20(18):849-50.
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    ABSTRACT: To evaluate the possibility and reliability of platysma pedicle flap transfer in the correction of hypopharynx stenosis,and delineate the operation skills and clinical results. Ten patients with hypopharynx stenosis underwent hypopharynx reconstruction using platysma pedicle flap. Nine of these patients had been decannulated successfully with good airway patency, good pronunciation and a good swallow. Platysma pedicle flap transfer was a single stage reconstruction, relatively simple procedure and less complication that can restore an adequate airway and a good swallow. The results indicate that the platysma pedicle flap is an ideal transplant for hypopharynx reconstruction. This method is simple, safe and reliable.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 09/2006; 20(15):694-5.
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    ABSTRACT: To investigate the effects of anteroposterior cricoid split interposition grafting on children with laryngotracheal stenosis. This is a retrospective study, from 1991 to 2001 years, 12 children (aged 6 years to 15 years) with laryngotracheal stenosis were operated with cricoid split laryngotracheal reconstruction at our hospital. All of 12 patients were tracheostomy dependent before reconstruction. Eleven patients (91.7%) were successfully decannulated with good airway patency and effective phonation. The follow-up period ranged from 1 to 10 years. The effect of operation is steady and the growth and development of children is normal. The anteroposterior cricoid split interposition grafting was a safe and effective method for treatment of laryngotracheal stenosis in children.
    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 07/2006; 20(12):529-30.
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    ABSTRACT: To study the success and failure factors with rib cartilage graft laryngotracheal reconstruction for severe laryngotracheal stenosis for improving the rate of successful decannulation. Thirty-six cases with severe laryngotracheal stenosis, received rib cartilage graft for laryngotracheal reconstruction during the last 18 years in our hospital, were analyzed retrospectively. Twenty-nine of the 36 patients (80.5%) were successfully decannulated after one procedure. The follow-up time ranged from 1 to 10 years. All of the 29 patients had a stable airway and normal exercise tolerance. The duration of T-tube stent was 10 days to one year (average 3 months). Seven patients (19.5%) failed to decannulate. The factors of failure were disturbance of local blood circulation, wound infection followed rib necrosis, T-tube injured mucomembrane then became a new stenosis, and restenosis for scar constitution. Laryngotracheal reconstraction with rib cartilage graft is one of effective method for severe laryngotracheal stenosis. The procedure is relatively simple, and shorter duration of stenting and higher rate of success. The drawbacks of this technique are ischemia necrosis easily occurred for isolated cartilage and required additional surgery for the donor sit. The patient with scar constitution or undertaken radiotherapy is contraindications. All the managements, appropriate selection of patients, diligent postoperative care, prevented wound infection and using T-tube properly, may increase the rate of successful decannulation. PJP].
    Zhonghua er bi yan hou ke za zhi 11/2002; 37(5):377-9.
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    ABSTRACT: To investigate the biologic characters of HPV positive laryngeal epithelial cells in vitro. To draw a cell growth curve by observing the biologic characters of HPV positive cells' growth pattern, PCR and nuclear acid hybridization were applied before and after culture for confirming HPV positive cells DNA. Primary HPV positive cells often remain viable in culture for more than six weeks, there have HPV DNA before and after culture. We found that in these cells had common characters: lag phase, growth phase and plateau phase. The cells culture showed that the first to forth day there have many cells emigration from tissue pieces. The fifth to Seventh day was latent phase, it was mainly adhere growth in this period, proliferation of culture cells wasn't active. The eighth to eighteenth day was growth phase, cells number was increased quickly, speed of growth was faster. Then cells growth entered to plateau phase, cells number was increased slowly, cells showed vacuoles obviously. It indicated that these cells came to die. Cells derives from HPV positive laryngeal epithelial have been successfully cultured, but to establish an animal model for HPV infection is difficult.
    Zhonghua er bi yan hou ke za zhi 09/2002; 37(4):287-90.
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    ABSTRACT: To assess the possibility of cartilage tissue engineering using human mesenchymal stem cells (hMSCs) and to investigate the quantitative relationship between hMSCs and engineered cartilage. Human mesenchymal stem cells were cultured, cryopreserved, and expanded in vitro. Surface antigens were detected by flow cytometry. In vitro chondrogenesis of hMSCs and cryopreserved hMSCs was performed. The chondrogenesis-induced hMSCs were seeded onto polyglycolic acid scaffolds, cultured in vitro for 3 weeks in chondrogenic medium, and then implanted into nude mice. The implants were harvested after 10 weeks and examined with histologic and immunochemical staining. The construction of cartilages was identified grossly and histologically: 1.9 to 2.5 x 10(7) nucleated cells were obtained from 1 mL of bone marrow, and about 1 to 2 x 10(6) hMSCs were obtained from the primary culture. The number of hMSCs tripled at every passage and reached 1.4 to 2.8 x 10(12) at passage 15. The purity of hMSCs was 95% and 98% at the primary and the fourth passages, respectively. Twenty-one days was the optimal (induction rate, 95%) induction time, with no apparent differences in induction rates among different passages. Based on our findings, hMSCs from 0.07 to 0.14 mL of bone marrow, expanded during 4 passages and induced for 21 days, would be sufficient to engineer 1 cm(2) of cartilage, 3-mm thick. Quantitative standards of hMSCs as seed cells for cartilage tissue engineering were established and may have value for later clinical work.
    Archives of Facial Plastic Surgery 7(1):7-11. · 1.46 Impact Factor