Jeannette Guarner

Emory University, Atlanta, Georgia, United States

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Publications (147)1043.5 Total impact

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    ABSTRACT: We evaluated the use of treponemal serum tests in cerebrospinal fluid (CSF) to diagnose neurosyphilis since CSF-Venereal Disease Research Laboratory (VDRL) is specific but lacks sensitivity. We tested CSF specimens using the following treponemal serum tests: INNO-LIA, Treponema pallidum particle agglutination (TP-PA), Trep-Sure, and Maxi-Syph. The reference standard to calculate sensitivity and specificity was having two or more reactive/positive tests on CSF. The reference standard group included 11 cases that fulfilled the definition of neurosyphilis (reactive CSF-VDRL plus symptoms) and three cases that did not fulfill the definition: two cases had neurologic symptoms but a nonreactive CSF-VDRL, and one had several positive CSF syphilis tests (reactive VDRL and positive treponemal and syphilis polymerase chain reaction) but no history (referred sample). Controls included 18 patients in whom a CSF-VDRL was performed the same week as patients in the reference group. The sensitivity was 85.7% (12/14) for CSF-VDRL, 92.9% (13/14) for Trep-Sure, 100% (10/10) for Maxi-Syph, 92.3% (12/13) for INNO-LIA, and 83.3% (10/12) for TP-PA. Specificity was 100% for all tests. Treponemal serum tests performed on CSF were useful in identifying two patients with nonreactive CSF-VDRL. Copyright© by the American Society for Clinical Pathology.
    American Journal of Clinical Pathology 04/2015; 143(4):479-84. DOI:10.1309/AJCPWSL3G8RXMCQR · 3.01 Impact Factor
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    ABSTRACT: To describe a 4-day laboratory medicine course for clinicians given at Addis Ababa University, Ethiopia, designed to improve the use of laboratory-based diagnoses. Each day was dedicated to one of the following topics: hematology, blood bank/transfusion medicine and coagulation, chemistry, and microbiology. The course included lectures, case-based learning, laboratory tours, and interactive computer case-based homework. The same 12-question knowledge quiz was given before and after the course. Twenty-eight participants took the quiz before and 21 after completing the course. The average score was 5.28 (range, 2-10) for the initial quiz and 8.09 (range, 4-11) for the second quiz (P = .0001). Two of 12 and 8 of 12 questions were answered correctly by more than 60% of trainees on the initial and second quiz, respectively. Knowledge and awareness of the role of the laboratory increased after participation in the course. Understanding of laboratory medicine principles by clinicians will likely improve use of laboratory services and build capacity in Africa. Copyright© by the American Society for Clinical Pathology.
    American Journal of Clinical Pathology 03/2015; 143(3):405-11. DOI:10.1309/AJCPNYT1WPSRCLC6 · 3.01 Impact Factor
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    ABSTRACT: Mycobacterium kansasii is a photochromogenic, slow-growing mycobacterium species that can cause pulmonary infection in patients with predisposing lung diseases, as well as extrapulmonary or disseminated disease in immunosuppressed patients. We describe a patient with a myelodysplastic syndrome, disseminated M kansasii infection, and ruptured aortic aneurysm. He had a recent diagnosis of mycobacterium cavitary lung lesions and was transferred to our facility for possible surgical intervention of an aortic aneurysm. Few hours after admission, the patient suddenly collapsed and died despite resuscitation efforts. A complete autopsy was performed and showed ruptured ascending aortic pseudoaneurysm with hemopericardium, disseminated necrotizing and nonnecrotizing granulomas with acid-fast bacilli in the aortic wall, lungs, heart, liver, spleen, and kidneys. Further genetic studies were consistent with monocytopenia and mycobacterial infection syndrome. Copyright © 2014 Elsevier Inc. All rights reserved.
    Human pathology 11/2014; 46(3). DOI:10.1016/j.humpath.2014.11.005 · 2.81 Impact Factor
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    ABSTRACT: Background: Microbiology rounds are an integral part of infectious disease consultation service. During microbiology rounds we highlight microbiology principles using vignettes. We created case-based, interactive, microbiology online modules similar to the vignettes presented during microbiology rounds since internal medicine residents rotating on our infectious disease elective have limited time to participate in rounds and learn microbiology. Objective: Evaluate the use of the microbiology online modules by internal medicine residents. Design: We asked residents to complete 10 of 25 online modules during their infectious disease elective. We evaluated which modules they chose and the change in their knowledge level. Results: Forty-six internal medicine residents completed assessments given before and after accessing the modules with an average of 11/20 (range 6-19) and 16/20 (range 9-20) correct questions, respectively (average improvement 5; p=0.0001). The modules accessed by more than 30 residents included those related to Clostridium difficile, anaerobes, Candida spp., Streptococcus pneumoniae, influenza, Mycobacterium tuberculosis, and Neisseria meningitidis. Summary: We demonstrated improved microbiology knowledge after completion of the online modules. This improvement may not be solely attributed to completing the online modules as fellows and faculty may have provided additional microbiology education during the rotation.
    Journal of Clinical Microbiology 11/2014; 53(1). DOI:10.1128/JCM.02696-14 · 4.23 Impact Factor
  • Jeannette Guarner · Kenneth Fleming
    American Journal of Clinical Pathology 10/2014; 142(4):435-6. DOI:10.1309/AJCPM9XQ4YHUWBEC · 3.01 Impact Factor
  • Jeannette Guarner
    American Journal of Clinical Pathology 10/2014; 142(4):428-30. DOI:10.1309/AJCPEPQB4G3ECCOP · 3.01 Impact Factor
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    Annals of internal medicine 08/2014; 161(10). DOI:10.7326/M14-1880 · 16.10 Impact Factor
  • Jeannette Guarner
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    ABSTRACT: The role pathology plays in establishing or excluding infectious diseases has been established. However, as the practice of pathology has become subspecialized, there is not enough infectious disease specimen volume to have a pathologist dedicated full time to this crosscutting subspecialty. So, what are the myths and realities of a practicing infectious disease pathologist in the hospital setting? Infectious disease clinicians tend to consult pathologists when there are questions regarding terminology used in pathology reports; when there is the need to perform additional studies on formalin-fixed, paraffin-embedded tissues; and when there is an interest in seeing biopsies or resections obtained from patients and in obtaining photographs for presentations. Pathologists consult infectious disease pathologists when there is a need to review diverse inflammatory reactions; for identification of fungi, parasites, or unknown structures; to define the need to use special stains and other techniques in order to identify organisms in tissues that have been formalin fixed; and to help with terminology to be used in reports. This review explores in more detail why and how these consultations occur.
    Clinical Infectious Diseases 06/2014; 59(8). DOI:10.1093/cid/ciu469 · 9.42 Impact Factor
  • Jeannette Guarner · Carlos del Rio
    American Journal of Clinical Pathology 05/2014; 141(6):770-771. DOI:10.1309/AJCPKM8XCG8WVVRL · 3.01 Impact Factor
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    ABSTRACT: Objectives: To describe the implementation and evaluation of a case-based microbiology curriculum during daily microbiology rounds. Methods: Vignettes consist of short cases with images and questions that facilitate discussion among microbiologists, pathologists, infectious disease physicians, and trainees (residents and fellows). We performed a survey to assess the value of these vignettes to trainees. Results: Motivation to come to rounds on time increased from 60% to 100%. Trainees attending rounds after implementation of the vignettes perceived the value of microbiology rounds to be significantly higher compared with those who attended rounds before implementation (P = .04). Pathology residents found that vignettes were helpful for retaining knowledge (8.3 of 10 points). Conclusions: The vignettes have enhanced the value of microbiology rounds by serving as a formalized curriculum exposing trainees from multiple specialties to various microbiology topics. Emphasis on interdisciplinary interactions between clinical and laboratory personnel was highlighted with this case-based curriculum.
    American Journal of Clinical Pathology 03/2014; 141(3):318-22. DOI:10.1309/AJCPW71HRNSSBYPO · 3.01 Impact Factor
  • 48th Annual Meeting of; 09/2013
  • 48th Annual Meeting of; 09/2013
  • Journal of clinical microbiology 07/2013; 51(7):2017. DOI:10.1128/JCM.01525-12 · 4.23 Impact Factor
  • Journal of clinical microbiology 07/2013; 51(7):2473. DOI:10.1128/JCM.01526-12 · 4.23 Impact Factor
  • Kathleen T Montone · Jeannette Guarner
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    ABSTRACT: Fungal infections are a frequent occurrence in medical practice due to increasing numbers of immunosuppressed patients. New antifungal medications have been developed and it has become evident that different fungi require different treatments as some are intrinsically resistant to these drugs. Thus, it is imperative that pathologists recognize the limitations of histopathologic diagnosis regarding speciation of fungal infections and advocate for the use of different techniques that can help define the genus and species of the fungus present in the specimen they are studying. In this review we present the use of in situ hybridization as an important adjunct for the diagnosis of fungal diseases, the different techniques that have been used for fungal identification, and the limitations that these techniques have.
    Advances in anatomic pathology 05/2013; 20(3):168-74. DOI:10.1097/PAP.0b013e31828d187d · 3.10 Impact Factor
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    ABSTRACT: Teaching microbiology to medical students is usually done by basic science microbiologists or infectious diseases physicians with minimal input from the laboratory specialists who validate, implement and perform these assays, and ensure quality control in clinical microbiology laboratories. In 2012, Emory Medical School developed, implemented, and evaluated a 4-hour clinical microbiology elective offered during the final 4-week module of medical school. A tour of the clinical microbiology laboratory occurred the day before. We selected 8 case-based vignettes with questions that emphasized laboratory techniques required to establish the diagnosis. An infectious diseases clinician and a clinical microbiologist or pathologist discussed the vignettes with groups of 6 to 8 medical students. Twenty-four of 30 students that participated in the elective answered a 14 question multiple-choice test before and after the session. The average score before the elective and at completion was 53% (range 36 - 71%) and 85% (64 - 100%) respectively. The average improvement was 4.75 (mode 4) correct questions with a minimum of 3 and a maximum improvement of 7. Although the assessment measured a limited amount of recently acquired knowledge, it is encouraging that students improved in their knowledge after this activity. The combination of the tour of the clinical microbiology laboratory and case-based discussions can give learners a better understanding of the function of the clinical laboratories, the complexity of microbiologic testing and the value of interacting with the professionals in the laboratory to enhance clinical care and guide appropriate antimicrobial selection.
    Journal of Medical Microbiology 04/2013; 62(Pt_7). DOI:10.1099/jmm.0.056481-0 · 2.27 Impact Factor
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    ABSTRACT: The frequency of fatalities due to acute bacterial meningitis has decreased significantly due to vaccinations, early diagnoses, and treatments. We studied brain tissues of patients with fatal neutrophilic meningitis referred to the Centers for Disease Control for etiologic diagnosis from 2000-2009 to highlight aspects of the disease that may be preventable or treatable. Demographic, clinical, and laboratory data were extracted from records. Of 117 cases in the database with a diagnosis of meningitis or meningoencephalitis, 39 had neutrophilic inflammation in the meninges. Inflammatory cells infiltrated the superficial cortex in 16 of 39 (41%) cases. Bacteria were found using Gram and bacterial silver stains in 72% of cases, immunohistochemistry in 69% (including two cases where the meningococcus was found outside the meninges), and PCR in 74%. Streptococcus pneumoniae was the cause of the meningitis in 14 patients and Neisseria meningitidis in 9. In addition, Streptococcus spp. were found to be the cause in six cases, while Staphylococcus aureus, Staphylococcus spp., Enterococcus spp., and Fusobacterium were the cause of one case each. There were six cases in which no specific etiological agent could be determined. The mean age of the patients with S. pneumoniae was 39 years (range 0-65), with N. meningitidis was 19 years (range 7-51), whereas that for all others was 31 years (range 0-68). In summary, our study shows that S. pneumoniae continues to be the most frequent cause of fatal neutrophilic bacterial meningitis followed by N. meningitidis, both vaccine preventable diseases.Modern Pathology advance online publication, 5 April 2013; doi:10.1038/modpathol.2013.30.
    Modern Pathology 04/2013; 26(8). DOI:10.1038/modpathol.2013.30 · 6.36 Impact Factor
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    ABSTRACT: Haemophilus influenzae rarely causes acute endometritis and the few published cases have always been associated with intrauterine devices (IUD). A 48-year-old female presented to the emergency department with a 3-day history of lower abdominal pain and fever. On physical examination she was tachycardic, hypotensive and had fundic tenderness to palpation. Imaging showed uterine leiomyomas and no IUD. Blood cultures grew a non-typable H. influenzae. Endometrial biopsy demonstrated acute endometritis. Tissue Gram stains and cervico-vaginal cultures were negative; however, polymerase chain reaction (PCR) determined presence of H. influenzae on the formalin-fixed, paraffin-embedded tissue biopsy. Evidence of H. influenzae in the endometrium demonstrates that the uterus can be the nidus for sepsis when invasive H. influenzae is found with no distinct usual primary focus. This case underscores the importance pathologic diagnosis and molecular testing.
    Diagnostic microbiology and infectious disease 03/2013; 76(2). DOI:10.1016/j.diagmicrobio.2013.02.010 · 2.57 Impact Factor
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    ABSTRACT: Deep eosinophilic granulomatous abscesses, as distinguished from eosinophilic subcutaneous abscesses, are rare. Most reports are from the Far-East and India where the most commonly attributed cause is Toxocara. Sulaimaniyah in Northeastern Iraq has experienced an outbreak of eosinophilic granulomatous liver and gastrointestinal (GI) abscesses beginning in 2009. The purpose of this study was to determine the etiology and guide treatment. The study was an ongoing investigation of patients having a histopathologic diagnosis of eosinophilic granulomatous abdominal abscesses in Sulaimaniyah hospitals from May 2009 to August 2012. Tissues were examined for organisms, and Enzyme Linked Immunoabsorbent Assays (ELISA) were performed for serum antibodies to Fasciola hepatica, Toxocara, and Echinococcus granulosus. Fourteen patients had granulomatous inflammation surrounding a central necrotizing eosinophilic exudate identified in surgical pathology specimens from abdominal surgeries. Two children and four adults had abscesses that formed GI masses. These patients included a 39 year old male with oropharyngeal and transverse colon disease, and a 48 year old male with liver and GI abscesses. All sites demonstrated a Zygomycete fungus surrounded by eosinophilic Splendori-Hoeppli material consistent with basidiobolomycosis. Five of the six patients with fungal disease were treated by surgery and 4 to 7 months of itraconozol. One child died of intestinal perforation while receiving IV amphotericin B; two adults required additional surgery for recurrent GI obstruction. Eight patients had isolated liver abscesses with no organisms identified by histopathology: ELISA results for F. hepatica were positive for five, borderline for one, and negative for two patients. These eight patients were treated for fascioliasis by surgical resection of localized abscesses and albendazol. One patient serologically positive for F. Hepatica was found to have a common duct fluke two years after initial diagnosis. Serological testing for Toxocara and Echinococcus granulosus was negative in all 14 patients. Basidiobolomycosis and F. hepatica are implicated as the cause of abdominal eosinophilic granulomatous abscesses in 12 of 14 patients identified over a period of 40 months in northern Iraq. Treatment was complicated by chronic biliary tract disease in fascioliasis and perforation and recurrent intestinal obstruction with basidiobolomycosis.
    BMC Infectious Diseases 02/2013; 13:91. DOI:10.1186/1471-2334-13-91 · 2.61 Impact Factor
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    Jeannette Guarner
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    ABSTRACT: Granuloma is an organized aggregate of immune cells that under the microscope appear as epithelioid macrophages. A granuloma can only be diagnosed when a pathologist observes this type of inflammation under the microscope. If a foreign body or a parasite is not observed inside the granuloma, stains for acid-fast bacilli and fungi are ordered since mycobacteria and fungi are frequently the cause of this type of inflammation. It is calculated that 12 to 36% of granulomas do not have a specific etiology and many have wondered if with new molecular methods we could reduce this number. This paper will summarize the frequently known causes of granulomas and will present the recent literature regarding the use of molecular techniques on tissue specimens and how these have helped in defining causative agents. We will also briefly describe new research regarding formation and function of granulomas and how this impacts our ability to find an etiologic agent.
    12/2012; 2012:494571. DOI:10.6064/2012/494571

Publication Stats

7k Citations
1,043.50 Total Impact Points


  • 1990–2015
    • Emory University
      • • Department of Pathology and Laboratory Medicine
      • • School of Medicine
      Atlanta, Georgia, United States
  • 2013
    • University of Pennsylvania
      Philadelphia, Pennsylvania, United States
  • 2009
    • University of Maryland, Baltimore
      • Department of Pediatrics
      Baltimore, Maryland, United States
  • 2000–2009
    • Centers for Disease Control and Prevention
      • • Division of Vector-Borne Diseases
      • • National Center for Emerging and Zoonotic Infectious Diseases
      Atlanta, Michigan, United States
  • 2001–2006
    • Stanford University
      • Department of Statistics
      Palo Alto, California, United States
    • University of New Mexico
      Albuquerque, New Mexico, United States
  • 2005
    • United States Army Medical Research Institute for Infectious Diseases
      Фредерик, Maryland, United States
  • 2004
    • University of New Mexico Hospitals
      Albuquerque, New Mexico, United States
  • 2003
    • University of Malaya
      Kuala Lumpor, Kuala Lumpur, Malaysia
    • Case Western Reserve University
      Cleveland, Ohio, United States
  • 1997–2002
    • Instituto Nacional de Cancerología - Mexico
      Ciudad de México, The Federal District, Mexico
  • 1998–2001
    • National Institute of Allergy and Infectious Diseases
      Maryland, United States