John Van Camp

Ghent University, Gent, VLG, Belgium

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Publications (86)180.27 Total impact

  • Article: Prenatal Lipid-Based Nutrient Supplements Increase Cord Leptin Concentration in Pregnant Women from Rural Burkina Faso.
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    ABSTRACT: In developing countries, prenatal lipid-based nutrient supplements (LNSs) were shown to increase birth size; however, the mechanism of this effect remains unknown. Cord blood hormone concentrations are strongly associated with birth size. Therefore, we hypothesize that LNSs increase birth size through a change in the endocrine regulation of fetal development. We compared the effect of daily prenatal LNSs with multiple micronutrient tablets on cord blood hormone concentrations using a randomized, controlled design including 197 pregnant women from rural Burkina Faso. Insulin-like growth factors (IGF) I and II, their binding proteins IGFBP-1 and IGFBP-3, leptin, cortisol, and insulin were quantified in cord sera using immunoassays. LNS was associated with higher cord blood leptin mainly in primigravidae (+57%; P = 0.02) and women from the highest tertile of BMI at study inclusion (+41%; P = 0.02). We did not find any significant LNS effects on other measured cord hormones. The observed increase in cord leptin was associated with a significantly higher birth weight. Cord sera from small-for-gestational age newborns had lower median IGF-I (-9 μg/L; P = 0.003), IGF-II (-79 μg/L; P = 0.003), IGFBP-3 (-0.7 μg/L; P = 0.007), and leptin (-1.0 μg/L; P = 0.016) concentrations but higher median cortisol (+18 μg/L; P = 0.037) concentrations compared with normally grown newborns. Prenatal LNS resulted in increased cord leptin concentrations in primigravidae and mothers with higher BMI at study inclusion. The elevated leptin concentrations could point toward a higher neonatal fat mass.
    Journal of Nutrition 03/2013; · 3.92 Impact Factor
  • Article: What nutrition label to use in a catering environment? A discrete choice experiment
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    ABSTRACT: Worksite and university canteens are increasingly used for daily main meal consumption. Following the use of front-of-pack nutrition labels on pre-packed foods, the provision of easily accessible nutrition information on foods prepared and consumed out of home is a highly topical policy issue with the potential to help consumers making better informed and more healthy food choices when eating out. Information that is presented in a format preferred by the target group is more likely to be used. A sample of 1725 university canteen users participated in a web-based choice experiment designed to identify and understand individual preferences for alternative nutrition labels on canteen meals. Participants valued the presence of nutrition labels on canteen meals and showed a preference for more detailed formats. Ability and motivation to process information as well as socio-demographics explained differences in label preferences. Observed decreasing marginal utility from combinations of two simple label for
    Food Policy 12/2012; 37(6):741-750. · 2.05 Impact Factor
  • Article: Explaining the effects of a point-of-purchase nutrition-information intervention in university canteens: a structural equation modelling analysis.
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    ABSTRACT: BACKGROUND: The importance of canteen meals in the diet of many university students makes the provision of simple point-of-purchase (POP) nutrition information in university canteens a potentially effective way to promote healthier diets in an important group of young adults. However, modifications to environments such as the posting of POP nutrition information in canteens may not cause an immediate change in meal choices and nutrient intakes. The present study aimed at understanding the process by which the POP nutrition information achieved its effects on the meal choice and energy intake, and whether the information was more effective in changing the meal choice of subgroups of university canteen customers. METHODS: The POP nutrition-information intervention used a one-group pretest-posttest design. A sample of 224 customers of two university canteens completed the baseline and 6-months follow-up surveys. A multi-group structural equation modelling analysis was used to test mediation effects of individual difference variables (liking, understanding and use of the information, subjective knowledge and attitude) on the energy intake from canteen meals, moderated by the objective nutrition knowledge and motivation to change diet. RESULTS: Significant relations were identified between liking of the information and its use on one hand and a positive effect in attitude towards healthy canteen meals on the other hand. Motivation to change diet and sufficient objective nutrition knowledge were required to maintain a recommended energy intake from canteen meals or to lead to a decrease in energy intake. Participants with greater objective nutrition knowledge had a greater understanding of the POP nutrition information which also resulted in a more effective use of the information. CONCLUSIONS: The results suggest that nutrition-information interventions may be more effective when using nutrition information that is generally liked by the target population in combination with an educational intervention to increase objective nutrition knowledge.Trial registrationNCT01249508.
    International Journal of Behavioral Nutrition and Physical Activity 09/2012; 9(1):111. · 3.83 Impact Factor
  • Article: Simplified nutrient labelling: consumers’ perceptions in Germany and Belgium
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    ABSTRACT: Growing consumer interest in food and health has motivated the European food industry to provide more simple information about the nutritional composition of foods. In addition to the traditional back-of-pack nutrition table, simplified front-of-pack labels have been introduced by the food industry to allow consumers making better informed and healthier food choices. In this study, consumers’ perceptions of simplified nutrition information, namely Guideline Daily Amount (GDA) and Traffic light (TL), in Germany and Belgium are explored. Consumer surveys in Germany (n=147) and Belgium (n=128) were conducted in 2008. Data were analysed by means of descriptive statistics and regression analysis. In both countries, the GDA is the most widely used simplified nutrition label. Whereas most consumers in Belgium indicate a preference for the GDA, in Germany the Traffic light is favoured most. Regression analyses indicate that the predilection for the different labels is affected by socio-demographic characteristics and perceptions towards the respective labels. European nutrition policy makers and food industries should be aware of cross-country differences regarding the perception of simplified nutrition labels. The challenge for both stakeholder groups is to raise awareness of the potential function of simplified labels in making informed and healthy food choices among European consumers. KeywordsNutrient profile labelling-Nutrition policy-European food industry-Consumer-Survey
    Journal für Verbraucherschutz und Lebensmittelsicherheit 04/2012; 5(2):169-180. · 0.63 Impact Factor
  • Article: Effect of decontamination on the microbial load, the sensory quality and the nutrient retention of ready-to-eat white cabbage
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    ABSTRACT: The effect of different decontamination treatments such as washing with sodium hypochlorite (20 and 200mg/L), peroxyacetic acid (80 and 250mg/L), neutral electrolysed oxidising water (4.9 and 31.7mg/L free chlorine) and contact with 1.55mg/L chlorine dioxide gas on the microbial and sensory quality, and the nutrient content of fresh-cut white cabbage was studied. Only rinsing with 200mg/L sodium hypochlorite, peroxyacetic acid or contact with gaseous chlorine dioxide resulted in significantly higher reductions of the total plate count (1.5–2.5 log cfu/g) than the ones achieved by washing with tap water (0.5 log cfu/g). However, those treatments giving the best results from a microbial point of view induced significant changes in the sensory quality. Regarding the effects on nutrient content, the mechanical effects caused by water washing already reduced the vitamin C content by 16–29%. Contrary to washing with neutral electrolysed oxidising water and contact with chlorine dioxide gas, a supplementary decrease of the vitamin C content ranging between 9 and 28% was observed, when peroxyacetic acid or 200mg/L sodium hypochlorite were used. After the use of peroxyacetic acid or gaseous chlorine dioxide, the phenol content also showed a decreasing trend, although not statistically significant. Apart from the effect of washing with water, the lipophilic nutrients were well retained after a decontamination step except for the α-tocopherol content, when peroxyacetic acid was used (−43 to 56%), and for the all-trans-β-carotene content (−8%) of cabbage in contact with gaseous chlorine dioxide. Because of its potential to reduce the initial microbial load without negative effects on the sensory quality, in combination with its limited effects on nutrient content, a treatment with 80mg/L peroxyacetic acid is preferable to decontaminate fresh-cut white cabbage.
    European Food Research and Technology 04/2012; 229(3):443-455. · 1.57 Impact Factor
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    Article: Protein-derived bioactives affecting CCK-induced satiety How to detect untargeted compounds and determine their identity
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    ABSTRACT: M o n o g r a ph ic s u pplem e n t s e r i e s ABSTRACT: Cholecystokinin (CCK) is a gut hormone that induces a feeling of satiety and therefore plays an important role in weight regulation. In this minireview, different food proteins and protein hydrolysates will be introduced that are able to enhance CCK-mediated satiety. This mechanism can be influenced at three interaction points, namely CCK release, trypsin-sensitive luminal CCK-releasing factors and the CCK receptor. Food components able to affect satiety are very promising ingredients in the development of functional foods that can be used as an aid in the battle against obesity.
    Agro Food Industry Hi Tech 03/2012; · 0.22 Impact Factor
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    Article: Screening of soy and milk protein hydrolysates for their ability to activate the CCK1 receptor.
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    ABSTRACT: The cholecystokinin receptor-type 1 (CCK1R) is a G-protein coupled receptor localized in the animal gastrointestinal tract. Receptor activation by the natural peptide ligand CCK leads to a feeling of satiety. In this study, hydrolysates from soy and milk proteins were evaluated for their potential to activate the CCK1R, assuming that bioactive peptides with a satiogenic effect can be used as an effective therapeutic strategy for obesity. Different protein hydrolysates were screened with a cell-based bioassay, which relies on the generation of a fluorescent signal upon receptor activation. Fluorescence was monitored using a fluorescence plate reader and confocal microscopy. Results from the fluorescence plate reader were biased by background autofluorescence of the protein hydrolysate matrices, which makes the fluorescence plate reader inappropriate for the evaluation of complex formulations. Measurements with the confocal microscope resulted in reliable and specific results. The latter approach showed that the gastrointestinal digested 7S fraction of soy protein demonstrates CCK1R activity.
    Peptides 11/2011; 34(1):226-31. · 2.43 Impact Factor
  • Article: The impact of photo-induced molecular changes of dairy proteins on their ACE-inhibitory peptides and activity.
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    ABSTRACT: Among all dietary proteins, dairy proteins are the most important source of bio-active peptides which can, however, be affected by modifications upon processing and storage. Since it is still unknown to which extent the biological activity of dairy proteins is altered by chemical reactions, this study focuses on the effect of photo-induced molecular changes on the angiotensin I converting enzyme (ACE) inhibitory activity. Milk proteins were dissolved in phosphate buffer containing riboflavin and stored under light at 4 °C for one month during which the molecular changes and the ACE-inhibitory activity were analysed. An increase in the total protein carbonyls and the N-formylkynurenine content was observed, besides a decrease in the free thiol, tryptophan, tyrosine and histidine content. These changes were more severe in caseins compared with whey proteins and resulted moreover in the aggregation of caseins. Due to these photo-induced molecular changes, a significant loss of the ACE-inhibitory activity was observed for casein peptides. A peptide analysis moreover illustrated that the decreased activity was not attributed to a reduced digestibility but to losses of specific ACE-inhibitory peptides. The observed molecular changes, more specifically the degradation of specific amino acids and the casein aggregation, could be assigned as the cause of the altered peptide pattern and as such of the loss in ACE-inhibitory activity.
    Amino Acids 11/2011; 43(2):951-62. · 3.25 Impact Factor
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    Article: 2012 Peptides Staljanssens soy-milk protein hydrolysates CCK1 receptor
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    ABSTRACT: Peptides j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / p e p t i d e s a b s t r a c t The cholecystokinin receptor-type 1 (CCK1R) is a G-protein coupled receptor localized in the animal gas-trointestinal tract. Receptor activation by the natural peptide ligand CCK leads to a feeling of satiety. In this study, hydrolysates from soy and milk proteins were evaluated for their potential to activate the CCK1R, assuming that bioactive peptides with a satiogenic effect can be used as an effective therapeu-tic strategy for obesity. Different protein hydrolysates were screened with a cell-based bioassay, which relies on the generation of a fluorescent signal upon receptor activation. Fluorescence was monitored using a fluorescence plate reader and confocal microscopy. Results from the fluorescence plate reader were biased by background autofluorescence of the protein hydrolysate matrices, which makes the flu-orescence plate reader inappropriate for the evaluation of complex formulations. Measurements with the confocal microscope resulted in reliable and specific results. The latter approach showed that the gastrointestinal digested 7S fraction of soy protein demonstrates CCK1R activity.
    Peptides 11/2011; 34:226-231. · 2.43 Impact Factor
  • Article: Strategies to reduce exposure of fumonisins from complementary foods in rural Tanzania.
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    ABSTRACT: Feeding infants with maize can expose them to fumonisin mycotoxins. We assessed fumonisin exposure from complementary foods in rural Tanzania and determined strategies to reduce the exposure. We conducted a cross-sectional study in four villages of Tarakea division, Northern Tanzania. We used a repeat 24-hour dietary recall to collect data of maize consumption as complementary food for 254 infants aged 6-8 months. Fumonisin concentrations in the maize were also estimated. Fumonisin exposure was assessed using @risk analysis software. With the software, several maximum fumonisin contamination and maize consumption patterns were combined in order to determine effective strategies for minimizing fumonisin exposure. Of the infants, 89% consumed maize at amounts up to 158g/person/day (mean; 43g/person/day±28). The maize was contaminated with fumonisins at levels up to 3201µgkg(-1) . Risk of fumonisin intake above the provisional maximum tolerable daily limit of 2µgkg(-1) body weight was 15% (95% confidence interval; 10-19). The risk was minimized when the maximum contamination was set at 150µgkg(-1) . The risk was also minimized when the maximum consumption was set at 20g/child/day while keeping the maximum contamination at the European Union (EU) maximum tolerated limit (MTL) of 1000µgkg(-1) . Considering the economical and technological limitations of adopting good agricultural practices in rural Tanzania, it is practically difficult to reduce contamination in maize to 150µgkg(-1) . We suggest adoption of the EU MTL of 1000µgkg(-1) for fumonisins in maize and reduction, by replacement with another cereal, of the maize component in complementary foods to a maximum intake of 20g/child/day.
    Maternal and Child Nutrition 11/2011; 8(4):503-11. · 1.61 Impact Factor
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    Article: Dietary sources of animal and plant protein intake among Flemish preschool children and the association with socio-economic and lifestyle-related factors.
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    ABSTRACT: The aims of this study were to assess the intake of animal, plant and food group-specific protein, and to investigate their associations with socio-economic and lifestyle-related factors in Flemish preschoolers. Three-day estimated dietary records were collected from 661 preschoolers aged 2.5-6.5 y (338 boys and 323 girls). Multiple linear regression analysis was used to investigate the association between animal, plant, and food group-specific protein intake and socio-economic and lifestyle factors. Animal proteins (mean 38 g/d) were the main source of total protein (mean 56 g/d), while mean plant protein intake amounted to 18 g/d. The group of meat, poultry, fish and eggs was the main contributor (51%) to animal protein intake, followed by milk and milk products (35%). Bread and cereals (41%) contributed most to the plant protein intake, followed by low-nutritious, energy-dense foods (21%). With higher educated fathers and mothers as reference, respectively, preschoolers with lower secondary and secondary paternal education had lower animal, dairy-, and meat-derived protein intakes, and those with lower secondary and secondary maternal education consumed less plant, and bread and cereal-derived proteins. Compared to children with high physical activity levels, preschoolers with low and moderate physical activity had lower animal and plant protein intakes. Significantly higher potatoes and grains-, and fish- derived proteins were reported for children of smoking mothers and fathers, respectively, compared to those of non-smoking mothers and fathers. The total protein intake of Flemish preschoolers was sufficient according to the recommendations of the Belgian Superior Health Council. Parental level of education and smoking status might play a role in the sources of children's dietary proteins.
    Nutrition Journal 09/2011; 10:97. · 2.48 Impact Factor
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    Article: Posting point-of-purchase nutrition information in university canteens does not influence meal choice and nutrient intake.
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    ABSTRACT: Growing concern over the relation between out-of-home eating and overweight has triggered the use of point-of-purchase (POP) nutrition information when eating out of the home. In canteens that offer various unhealthy choices, the posting of POP nutrition information has the potential to improve meal choices and dietary intakes. The objective of this study was to increase the proportion of consumed meals that comply with recommendations for energy, saturated fat, sodium, and vegetable content by 5%. A one-group pretest-posttest design was used. A total of 224 customers of 2 university canteens completed a questionnaire used for consumer profiling and 3-d food records to assess their meal choices and nutrient intakes. The 12 best meal combinations received star ratings and descriptors for nutrients or food groups that did not comply. Reported meal choices in canteens and nutrient intakes did not improve after the intervention (P > 0.05). The nutritional profile of the meal choice, obtained from a qualitative and quantitative nutritional assessment of meals, mirrored the nutritional profile of all meals offered (P > 0.05) and not that of the recommended meals offered (P < 0.001). Meal choices were not compensated for later in the day (P > 0.05). The healthiest choices were made by participants with greater objective nutrition knowledge, stronger health and weight-control motives, and a greater openness to change meal choices at baseline (P < 0.05). The posting of nutrition information in university canteens did not effectively change meal choices and nutrient intakes. Despite the intervention, meal choices were largely determined by meals offered. Therefore, nutrition-information interventions in canteens may be more effective with a healthier meal supply. This trial was registered at clinicaltrials.gov as NCT01249508.
    American Journal of Clinical Nutrition 06/2011; 94(2):562-70. · 6.67 Impact Factor
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    Article: Antihypertensive effect of insect cells: in vitro and in vivo evaluation.
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    ABSTRACT: In this study, we investigated the in vitro ACE inhibitory and in vivo antihypertensive effect of insect cell extracts. The IC(50) of three insect cell lines from different type and insect species origin: S2 (embryo, Drosophila melanogaster), Sf21 (ovary, Spodoptera frugiperda) and Bm5 (ovary, Bombyx mori), were evaluated. Most interesting results were that the IC(50) values ranged between 0.4 and 0.9 mg/ml, and that an extra hydrolysis with gastrointestinal enzymes did not increase the ACE inhibitory activity conspicuously. Finally, a single oral administration with a gavage of 150 mg cell extract/kg BW to spontaneous hypertensive rats (SHR) significantly decreased (p<0.05) their systolic blood pressure (SBP) with 5-6% (9-12 mmHg) compared to the controls at 6 h post-administration. Here the undigested and digested insect S2 cell extracts were equal in activity to lower the SBP. To the best of our knowledge, this is the first report of in vivo antihypertensive activity of insect cell extracts and this without an extra digestion requirement.
    Peptides 03/2011; 32(3):526-30. · 2.43 Impact Factor
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    Article: Fibre intake among the Belgian population by sex-age and sex-education groups and its association with BMI and waist circumference.
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    ABSTRACT: The objectives of the present study were to assess total dietary fibre intake and the main contributors to fibre intake in the Belgian population by sex-age and sex-education groups and to investigate its relationship with BMI and waist circumference (WC). The participants of the Belgian food consumption survey (2004) were randomly selected. Information about food intake was collected using two repeated, non-consecutive 24 h recall interviews. A total of 3083 individuals ( ≥ 15 years; 1546 men and 1537 women) completed both interviews. The main contributors to total fibre intake (17·8 g/d) were cereals and cereal products (34 %; 5·9 g/d), potatoes and other tubers (18·6 %; 3·3 g/d), fruits (14·7 %; 2·8 g/d) and vegetables (14·4 %; 2·6 g/d). Legume fibre intake was extremely low (0·672 %; 0·139 g/d). In all sex-age and sex-education groups, total fibre intake was below the recommendations of the Belgian Superior Health Council. Men (21 g/d) consumed significantly more fibre than women (17·3 g/d) (P < 0·001). Lower educated men and higher educated women reported the highest fibre intake. A significant inverse association was found between total fibre intake and WC (β = - 0·118, P < 0·001). Fruit-derived fibre was positively associated with WC (β = 0·731, P = 0·001). In summary, total fibre intake was inversely associated with WC, whereas fruit-derived fibre intake was positively associated with WC in the Belgian population.
    The British journal of nutrition 03/2011; 105(11):1692-703. · 3.45 Impact Factor
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    Article: The CCK(-like) receptor in the animal kingdom: functions, evolution and structures.
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    ABSTRACT: In this review, the cholecystokinin (CCK)(-like) receptors throughout the animal kingdom are compared on the level of physiological functions, evolutionary basis and molecular structure. In vertebrates, the CCK receptor is an important member of the G-protein coupled receptors as it is involved in the regulation of many physiological functions like satiety, gastrointestinal motility, gastric acid secretion, gall bladder contraction, pancreatic secretion, panic, anxiety and memory and learning processes. A homolog for this receptor is also found in nematodes and arthropods, called CK receptor and sulfakinin (SK) receptor, respectively. These receptors seem to have evolved from a common ancestor which is probably still closely related to the nematode CK receptor. The SK receptor is more closely related to the CCK receptor and seems to have similar functions. A molecular 3D-model for the CCK receptor type 1 has been built together with the docking of the natural ligands for the CCK and SK receptors in the CCK receptor type 1. These molecular models can help to study ligand-receptor interactions, that can in turn be useful in the development of new CCK(-like) receptor agonists and antagonists with beneficial health effects in humans or potential for pest control.
    Peptides 03/2011; 32(3):607-19. · 2.43 Impact Factor
  • Article: Consumption of plant sterols in Belgium: estimated intakes and sources of naturally occurring plant sterols and β-carotene.
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    ABSTRACT: The objective of the present study was to assess the intake of naturally occurring plant sterols and β-carotene via the overall diet of Belgian pre-school children and adults. Two different Belgian food consumption databases were used: (1) one with consumption data of pre-school children (2·5-6·5 years old) and (2) one with consumption data of adults ( ≥ 15 years old). These consumption data were combined with a newly developed database containing the plant sterol and β-carotene content in all relevant food items based on international food composition databases and scientific literature. The results show that Flemish pre-school children have a median plant sterol intake of 172 (interquartile range (IQR) = 47) and 184 (IQR = 52) mg/d for girls and boys, respectively. Their median β-carotene intake was 1857 (IQR = 1250) μg/d, without significant difference between girls and boys. Belgian women and men have a median plant sterol intake of 218 (IQR = 113) and 280 (IQR = 158) mg/d, respectively, and a median β-carotene intake of 2086 (IQR = 1254) μg/d (not significantly different between the sexes). The main food source of naturally occurring plant sterols was bread and other cereal products. For β-carotene, the main food source was vegetables.
    The British journal of nutrition 03/2011; 105(6):960-6. · 3.45 Impact Factor
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    Article: 2012 Peptides Staljanssens CCK1 receptor
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    ABSTRACT: Peptides j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / p e p t i d e s Time-resolved quantitative analysis of CCK1 receptor-induced intracellular calcium increase a b s t r a c t Cholecystokinin (CCK) is a gastrointestinal hormone, which regulates many physiological functions such as satiety by binding to the CCK receptor (CCKR). Molecules, which recognize this receptor can mimic or block CCK signaling and thereby influence CCKR-mediated processes. We have set up a quantitative heterologous assay with CHO cells over-expressing the rat CCK1 receptor to screen for such candi-date molecules. Receptor activation, induced by agonist binding, is followed by an intracellular calcium increase, which was monitored using a fluorescent sensor dye. For quantification of the calcium increase, a population average technique using a fluorescence plate reader was optimized and subsequently com-pared with a single-cell approach using confocal microscopy. With both strategies, dose–response curves were generated for the natural agonist CCK-8S, the partial agonist JMV-180 as well as the antagonist lorglumide. Significant differences were found between the ligands and a strong correspondence was observed between both methods in terms of maximum response and median effect concentrations. Both highly sensitive methods proved complementary: whereas the plate reader assay allowed faster, high throughput screening, the confocal microscopy identified single-cell variations and revealed factors that reduce specificity and sensitivity.
    Peptides 02/2011; 34:219-225. · 2.43 Impact Factor
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    Article: Time-resolved quantitative analysis of CCK1 receptor-induced intracellularcalcium increase
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    ABSTRACT: Peptides j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / p e p t i d e s Time-resolved quantitative analysis of CCK1 receptor-induced intracellular calcium increase a b s t r a c t Cholecystokinin (CCK) is a gastrointestinal hormone, which regulates many physiological functions such as satiety by binding to the CCK receptor (CCKR). Molecules, which recognize this receptor can mimic or block CCK signaling and thereby influence CCKR-mediated processes. We have set up a quantitative heterologous assay with CHO cells over-expressing the rat CCK1 receptor to screen for such candi-date molecules. Receptor activation, induced by agonist binding, is followed by an intracellular calcium increase, which was monitored using a fluorescent sensor dye. For quantification of the calcium increase, a population average technique using a fluorescence plate reader was optimized and subsequently com-pared with a single-cell approach using confocal microscopy. With both strategies, dose–response curves were generated for the natural agonist CCK-8S, the partial agonist JMV-180 as well as the antagonist lorglumide. Significant differences were found between the ligands and a strong correspondence was observed between both methods in terms of maximum response and median effect concentrations. Both highly sensitive methods proved complementary: whereas the plate reader assay allowed faster, high throughput screening, the confocal microscopy identified single-cell variations and revealed factors that reduce specificity and sensitivity.
    Peptides 02/2011; 34:219-225. · 2.43 Impact Factor
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    Article: Time-resolved quantitative analysis of CCK1 receptor-induced intracellular calcium increase.
    [show abstract] [hide abstract]
    ABSTRACT: Cholecystokinin (CCK) is a gastrointestinal hormone, which regulates many physiological functions such as satiety by binding to the CCK receptor (CCKR). Molecules, which recognize this receptor can mimic or block CCK signaling and thereby influence CCKR-mediated processes. We have set up a quantitative heterologous assay with CHO cells over-expressing the rat CCK1 receptor to screen for such candidate molecules. Receptor activation, induced by agonist binding, is followed by an intracellular calcium increase, which was monitored using a fluorescent sensor dye. For quantification of the calcium increase, a population average technique using a fluorescence plate reader was optimized and subsequently compared with a single-cell approach using confocal microscopy. With both strategies, dose-response curves were generated for the natural agonist CCK-8S, the partial agonist JMV-180 as well as the antagonist lorglumide. Significant differences were found between the ligands and a strong correspondence was observed between both methods in terms of maximum response and median effect concentrations. Both highly sensitive methods proved complementary: whereas the plate reader assay allowed faster, high throughput screening, the confocal microscopy identified single-cell variations and revealed factors that reduce specificity and sensitivity.
    Peptides 02/2011; 34(1):219-25. · 2.43 Impact Factor
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    Article: Dietary Sources of Fiber Intake and Its Association with Socio-Economic Factors among Flemish Preschool Children.
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    ABSTRACT: The objectives were to assess total dietary fiber intake, identify the major sources of dietary fiber, and examine its association with socio-economic factors among Flemish preschoolers. Three-day estimated dietary records were collected from a representative sample of preschoolers 2.5-6.5 years old (n = 661; 338 boys, 323 girls). The mean dietary fiber intake (13.4 g/d) was lower than the intake level recommended by the Belgian Superior Health Council (70% boys and 81% girls below the guidelines). The most important contributor was the group of bread and cereals (29.5%), followed by fruits (17.8%), potatoes and grains (16.0%), energy-dense, low-nutritious foods (12.4%), and vegetables (11.8%). Multiple linear regression analyses showed that total fiber intake was associated with maternal education and parents' employment. Overall, fiber intakes from high-nutritious foods (vegetables and fruits) were higher in preschoolers of higher educated mothers and those with one or both parents being employed. In conclusion, the majority of the preschoolers had dietary fiber intakes below the recommended level. Hence, dietary fiber should be promoted among parents of preschoolers and low socio-economic status families should be addressed in particular.
    International Journal of Molecular Sciences 01/2011; 12(3):1836-53. · 2.60 Impact Factor

Institutions

  • 2000–2013
    • Ghent University
      • • Department of Food Safety and Food Quality
      • • Department of Public Health
      • • Department of Crop Protection
      • • Department of Agricultural Economics
      • • Laboratory of Microbiology
      Gent, VLG, Belgium
  • 2012
    • University of Antwerp
      Antwerpen, VLG, Belgium
  • 2005
    • KU Leuven
      • Faculty of Pharmaceutical Sciences
      Leuven, VLG, Belgium
  • 2004
    • University of Agricultural Sciences and Veterinary Medicine
      Cluj-Napoca, Judetul Cluj, Romania