V Vrbíková

Publications (3)2.85 Total impact

• Article: Mutagenicity of airborne particulate matter PM10.

  A Pastorková, M Cerná, J Smíd, V Vrbíková
  [show abstract] [hide abstract]
  ABSTRACT: Mutagenic activity of extractable organic matter (EOM), from airborne particles collected over winters in four towns of Czech Republic, was investigated by the means of Salmonella typhimurium indicator strains TA98 and YG1041 using the Ames plate incorporation assay. Mutagenicity of all tested samples showed significant dose-related increase in number of revertants per mg of EOM. The direct mutagenic potency detected with TA98 increased further in the presence of external metabolic activation. The mutagenic potency detected with YG1041 was about two orders of magnitude higher than that detected with TA98. The mutagenicity results correlated with the concentrations of the polycyclic aromatic hydrocarbons (PAHs) determined by GC/MS. Local differences in mutagenicity, expressed as numbers of revertants per m3 of air, were observed with the highest values in Prague air samples. For routine ambient air mutagenicity monitoring, the use of YG1041 and the plate incorporation test are recommendable.
  Central European journal of public health 04/2004; 12 Suppl:S72-5.
• Article: Mutagenicity monitoring of airborne particulate matter (PM10) in the Czech Republic.

  M Cerná, A Pastorková, V Vrbíková, J Smíd, P Rössner
  [show abstract] [hide abstract]
  ABSTRACT: The mutagenic activities associated with inhalable airborne particulate matter (PM10) collected over a year in four towns (Czech Republic) have been determined. The dichloromethane extracts were tested for mutagenicity using the Ames plate incorporation test and the Kado microsuspension test both with Salmonella typhimurium TA98 and its derivative YG1041 tester strains in the presence and absence of S9 mixture. The aim of this study was to assess the suitability of both bacterial mutagenicity tests and to choose the appropriate indicator strain for monitoring purposes. To elucidate the correlation between mutagenicity and polycyclic aromatic hydrocarbons (PAHs), the concentration of PAHs in the air samples were determined by GC/MS. In general, the significant mutagenicity was obtained in organic extracts of all samples, but differences according to the method and tester strain used were observed. In both mutagenicity tests, the extractable organic mass (EOM) exhibited higher mutagenicity in the YG1041 strain (up to 97 rev/microg in the plate incorporation and 568 rev/microg in the microsuspension tests) than those in TA98 (up to 2.2 rev/microg in the plate incorporation and 14.5 rev/microg in the microsuspension tests). In the plate incorporation test, the direct mutagenic activity in YG1041 was on average 60-fold higher and in microsuspension assay 45-fold higher with respect to strain TA98. In the presence of S9 mix, the mutagenic potency in YG1041 declined (P<0.001) in summer, but increased in TA98 (P<0.05) in samples collected during the winter season. The microsuspension assay provided higher mutagenic responses in both tester strains, but in both strains a significant decrease of mutagenic potency was observed in the presence of S9 mix (P<0.001 for YG1041, P<0.05 for TA98 in winter). The mutagenic potencies detected with both indicator strains correlated well (r=0.54 to 0.87) within each mutagenicity test used but not (for TA98) or moderately (r=0.44 to 0. 66 for YG1041) between both of the tests. The mutagenic activity (in rev/m(3)) likewise the concentration of benzo[a]pyrene and sum of carcinogenic PAHs showed seasonal variation with distinctly higher values during winter season. A correlation between the PAH concentrations and the mutagenicity results for the plate incorporation, but not for the microsuspension tests was found. In samples from higher industrial areas, the higher mutagenicity values were obtained in plate incorporation test with TA98 and in both tests with YG1041 in summer season (P<0.05). According to our results, plate incorporation test seems to be more informative than microsuspension assay. For routine ambient air mutagenicity monitoring, the use of YG1041 tester strain without metabolic activation and the plate incorporation test are to be recommended.
  Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 09/1999; 444(2):373-86. · 2.85 Impact Factor
• Article: Study of the genotoxicity of toluene.

  D Pelclová, M Cerná, A Pastorková, V Vrbíková, B Procházka, D Hurychová, Z Dlasková, M Hornychová
  [show abstract] [hide abstract]
  ABSTRACT: Chromosome analysis was conducted for peripheral lymphocytes of 23 printers exposed to toluene concentrations of 590 mg/m3 in a rotary machine workshop and to rotogravure printing inks. The percentages of aberrant cells were 2.30 in the printers and 1.46 in the control group (n = 22) (p < .05). The concentration of hippuric acid in printers was significantly higher than in the control group (p < .01), and the level of blood toluene at the end of the workshift was 0.500 mg/l. The authors also examined rotogravure printing inks-considered a potential source of genotoxic polycyclic aromatic hydrocarbons because they contained carbon black-their use in printing plants, and previous documentation of increased chromosomal aberrations in rotogravure printers. Only milligrams of fluorene and phenanthrene per gram of the printing inks were found; no polycyclic aromatic hydrocarbons with carcinogenic properties were discovered in the inks. The authors used Salmonella typhimurium indicator strains TA 98, TA 100, TA 1537, and YG 1041 in spot tests and indicator strains TA 98 and TA 100 in plate-incorporation assays to determine that there was no bacterial mutagenicity of all four colors of rotogravure inks. Urinary mutagenicity, which was evaluated with a microsuspension assay containing YG 1041 indicator strain both in the presence and absence of metabolic activation, was also studied. No significant difference in bacterial mutagenicity was found between the exposed and control groups. The increased percentage of aberrant cells in printers can be explained by exposure to genotoxicants that are not excreted in urine. Toluene was the most likely cause of the aberration.
  Archives of Environmental Health An International Journal 55(4):268-73.