Yu-Cheng Wang

Northeast Forestry University, Charbin, Heilongjiang Sheng, China

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Publications (10)6.87 Total impact

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    ABSTRACT: Glycine-rich RNA-binding proteins (GRPs) are involved in post-transcriptional regulation of genes, which have been found to play a role in stress response. However, whether GRPs can mediate some physiological responses related to salt stress tolerance is still not known. In the present study, we investigated the role of GRPs in salt stress-induced physiological responses by generating transgenic tobacco lines overexpressing a GRP (LbGRP1) gene from Limonium bicolor (Bunge) Kuntze. Compared with wild type (WT) tobacco, the transgenic plants showed significantly improved superoxide dismutase and catalase activities under salt stress conditions. Levels of proline in the transgenic plants were significantly higher than those in the WT plants grown under NaCl stress conditions. Furthermore, Na(+) content and Na(+)/K(+) ratio in the transgenic plants were lower than those in the WT plants under both normal growth and stress conditions. These results suggested that overexpression of the LbGRP1 gene can affect some physiological processes associated with salt tolerance of plants. Therefore, we hypothesize that LbGST1 can enhance stress resistance by mediating some physiological pathways.
    Molecular Biology Reports 05/2011; 39(2):1047-53. · 2.51 Impact Factor
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    ABSTRACT: Glycine-rich RNA-binding proteins (GRP) are involved in post-transcriptional regulation, and play important roles in plant growth, development and stress response. In the present study, the full-length cDNA of a GRP gene (LbGRP, GenBank accession number: GQ398238) was cloned from a cDNA library of Limonium bicolor. To investigate the stress tolerance of LbGRP, the recombinant plasmid pYES2-LbGRP was constructed by inserting the LbGRP gene into the yeast expression vector pYES2. The recombinant plasmid pYES2-LbGRP was transformed into yeast Saccharomyces cerevisiae INVSc1, and the INVSc1 strain transformed with empty pYES2 was used as the control. The recombinant yeast INVSc1 harboring LbGRP (pYES2-LbGRP) and the control INVSc1 harboring empty pYES2 were treated with NaCl, KCl, NaHCO3, Na2CO3, drought and frezzing, respectively, and their survial rates were compared under these stress conditions. The results showed that the survival rates of the recombinant yeast INVScl (pYES2-LbGRP) were higher than that of the control strain under these stress conditions, indicating that the LbGRP is tolerant to NaCl, KCl, NaHCO3, Na2CO3, drought and frezzing stresses. These results suggested that LbGRP plays a role in stress tolerance of L. bicolor.
    Hereditas (Beijing) 03/2010; 32(3):278-86.
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    ABSTRACT: The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS-PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were approximately 57, approximately 50 and approximately 47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
    Molecular Biology Reports 04/2009; 37(3):1183-90. · 2.51 Impact Factor
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    ABSTRACT: The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4 degrees C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 microM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana x P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.
    Biotechnology Letters 04/2009; 31(7):1079-87. · 1.85 Impact Factor
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    ABSTRACT: The full length cDNA of a novel metallothionein (LbMT2) gene was cloned from a cDNA library of Limonium bicolor. The LbMT2 gene cloned is 518 bp in length, which includes a 64 bp of 5' untranslated region (UTR) and a 205 bp of 3' untranslated region. This gene has an open reading frame (ORF) of 249 bp in length, encoding a protein of 82 amino acid residues with the molecular mass of 8.1 kDa and theoretical pI of 4.71. The expression of LbMT2 gene in L. bicolor in response to CuSO4, CdCl2, NaCl, cold, and PEG was further investigated using real time quantitative PCR. In both leaf and root of L. bicolor, the expression of LbMT2 was induced by CuSO4, CdCl2, NaCl, and cold, but inhibited by PEG stress. LbMT2 gene was inserted into a prokaryotic expression vector (pGEX-4T-2) to produce the recombinant expression vector pGEX-LbMT2. The expression of LbMT2 in E. coli BL21 was induced with IPTG, which produced a protein band with expected size of 35 kDa on SDS-PAGE.
    Hereditas (Beijing) 09/2008; 30(8):1075-82.
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    ABSTRACT: The role of late embryogenesis abundant (LEA) proteins in stress tolerance was examined by using a yeast expression system. LEA protein tolerance to the abotic stresses in plants involved in salt, drought and freezing stresses and additional tolerance to heat, Na-HCO3 (salt-alkali) and ultraviolet radiation was also investigated. The transgenic yeast harboring the Tamarix LEA gene (DQ663481) was generated under the control of inducible GAL promoter (pYES2 vector), yeast cells transformed with pYES2 empty vector were also generated as a control. Stress tolerance tests showed that LEA yeast transformants exhibited a higher survival rates than the control transformants under high temperature, NaHCO3, ultraviolet radiation, salt (NaCl), drought and freezing, indicating that the LEA gene is tolerant to these abiotic stresses. These results suggest that the LEA gene is resistant to a wider repertoire of stresses and may play a common role in plant acclimation to the examined stress conditions.
    Journal of Forestry Research 02/2008; 19(1):58-62.
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    ABSTRACT: Tamarix androssowii is one of the most remarkable salt-tolerance woody plant species in China. To investigate its expression profile in response to NaHCO3 stress, expressed sequence tags (ESTs) was analyzed. We have constructed a 0.4 mol/l NaHCO3 treated cDNA library of T. androssowii and sequenced more than 3000 randomly selected clones. A total of 2455 high-quality ESTs were generated, 1268 (51.65%) of these ESTs showed significant similarity to the sequences in NCBI Nr database. Nearly 400 ESTs were found to be involved in salt-tolerance; among them, metallothionein-like protein and germin-like protein are the most abundant transcripts, and account for 2.44 and 1.91% of total ESTs, respectively. The database-matched ESTs were further grouped into 12 function categories including: metabolism (19.72%), cell rescue, defense (20.19%), protein synthesis (7.33%), energy (5.12%), transport facilitation (6.78%), protein destination (6.94%), signal transduction (4.89%), cell growth, division (4.42%), transcription (6.62%), photosynthesis (9.94%), cell structure (1.66%) and function unknown (6.39%). Ninety-six unique genes were selected for small cDNA microarray analysis. Six were down-regulated and three up-regulated significantly. The experimental results obtained from this research will represent a key step in understanding the molecular mechanism of NaHCO3 tolerance of Tamarix species.
    Plant Science. 01/2006;
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    ABSTRACT: Gene chip technology was employed to study gene expression in Tamarix androssowii under NaHCO3 stress. cDNAs from T. androssowii treated with NaHCO3 solution and that from control group were labeled with fluorescent dye CyS and Cy3 respectively. The two fluorescent cDNA probes were mixed and hybridized to gene chips containing T. androssowii genes, and the chips were scanned using biochip scanning system. Differential expression of genes was analyzed through calculation of the ratio of Cy5 to Cy3 signal intensities. Total of 89 genes differentially expressed were identified, among them, 27 showed down regulated expression and 62 showed up regulated expression. Blastx analysis showed that the function of the differentially expressed genes could be grouped into some categorizations such as photosynthesis, reactive oxygen species eliminated, regulation of osmotic potential, regulation of gene expression and signal transduction, metabolism, development, ribosomal protein, protein breakdown and recycling, transporter, water channel proteins and so on. Based on this research, some function-unknown or novel unreported genes that respond to salt stress were also identified, and these genes may have important functions in salt resistance of T. androssowii. Some important pathways of salt resistance in T. androssowii are revealed, and the gene expression profiling of T. androssowii under salt stress and without stress is obtained in this study.
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology 04/2005; 21(2):220-6.
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    ABSTRACT: The gene expression of Tamarix androssowii under NaHCO3 stresses is studied by using SSH technique, in which the cDNA from the materials treated with NaHCO3 solution is as tester and the cDNA from the materials in normal growth is as driver. Total 36 genes related to NaHCO3 stress were obtained through Northern hybridization. Blastx analysis showed that the proteins encoded by these genes were homologous to the following proteins: the antioxidant enzymes catalase and peroxiredoxin; trehalose phosphatase, which was related to trehalose synthesis; a few regulation proteins such as bZIP transcription factor, MADS-box protein, glycine-rich RNA-binding proteins, CCCH-type zinc finger protein and F-box protein etc; early light-induced protein, which could protect and/or repair the photosynthetic apparatus damage induced by stress; cysteine proteinase and vacuolar processing enzyme that can make function in plant cell death, and lipid transfer protein precursor, polyubiquitin, chalcone synthase, NADP-dependent isocitrate dehydrogenase, salt-induced S12 protein, and oxygen-evolving enhancer protein 1 etc. Among 36 genes obtained, the proteins encoded three genes were homologous to 3 putative proteins: HAK2, calcium-binding protein and RNA-binding protein, respectively. In addition, 6 new salt stress response squences were found. The result indicated that the salt-tolerant mechanism of Tamarix androssowii may be a complicated, interactive system involving multiple approaches and multiple genes, but not only a single salt gland-depended approach.
    Acta Genetica Sinica 10/2004; 31(9):926-33.
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    ABSTRACT: Differential display technique was used to study the gene expression of Tamarix androssowii treated with NaHCO(3) solution. Seventeen differentially expressed genes were obtained through Northern detection. The result of BLASTX analysis shows that two of these genes (GenBank accession number CD028574 and CD028579) are homologous to the F-box protein family member, which can regulate diverse cellular processes, including cell cycle transition, transcription regulation and signal transduction, by playing roles in Skp1p-cullin-F-box protein (SCF) complexes or non-SCF complexes. F-box protein plays a very important role in plant defence system. One gene (GenBank accession number CD028589) is highly homologous to the translation initiation factor eIF, which regulates the proteins translation and is an important determinant of sodium tolerance in yeast and plants. Additionally, two genes (GenBank accession numbers CD028584 and CD028586) that are homologous to secretory peroxidase and peroxidases were identified, peroxidases are familiar proteins expressed in plant under stress. Five new genes closely related to salt and alkali resistance were obtained, which were expressed quite differentially when treated by NaHCO(3) solution. Seven differentially expressed genes were obtained which have highly or some homologous to the known genes, their function in stress need to be further studied. These seventeen genes were accepted as novel cDNA sequences by GenBank and were given them new accession numbers, their accession numbers were CD028574- CD028581 and CD028583-CD028591.
    Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology 05/2004; 30(2):229-33.

Publication Stats

36 Citations
1 Download
663 Views
6.87 Total Impact Points

Institutions

  • 2006–2011
    • Northeast Forestry University
      • • State Key Laboratory of Tree Breeding
      • • College of Forestry
      Charbin, Heilongjiang Sheng, China