Sandrine Bernabeu

Publications (17)92.24 Total impact

• Article: Occurrence of OXA-48 and VIM-1 carbapenemase-producing Enterobacteriaceae in Egypt.

  Laurent Poirel, Mohammed O Abdelaziz, Sandrine Bernabeu, Patrice Nordmann
  International journal of antimicrobial agents 10/2012; · 3.03 Impact Factor
• Article: Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae.

  Sandrine Bernabeu, Laurent Poirel, Patrice Nordmann
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  ABSTRACT: Carbapenem-hydrolyzing ß-lactamases are the most powerful ß-lactamases being able to hydrolyse almost all ß-lactams. They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- from noncarbapenemase producers. This inexpensive technique adapted to screening of carbapenemase producers may be implemented in any reference laboratory worldwide.
  Diagnostic microbiology and infectious disease 06/2012; 74(1):88-90. · 2.45 Impact Factor
• Article: Importation of OXA-48-producing Klebsiella pneumoniae from Kuwait.

  Laurent Poirel, Etienne Carbonnelle, Sandrine Bernabeu, Laurent Gutmann, Vincent Rotimi, Patrice Nordmann
  Journal of Antimicrobial Chemotherapy 05/2012; 67(8):2051-2. · 5.07 Impact Factor
• Article: NDM-1-producing Klebsiella pneumoniae in Mauritius.

  Laurent Poirel, Christine Lascols, Sandrine Bernabeu, Patrice Nordmann
  Antimicrobial Agents and Chemotherapy 01/2012; 56(1):598-9. · 4.84 Impact Factor
• Source

  Available from: Laurent Dortet

  Article: Genetic features of blaNDM-1-positive Enterobacteriaceae.

  Laurent Poirel, Laurent Dortet, Sandrine Bernabeu, Patrice Nordmann
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  ABSTRACT: Genetic features associated with the bla(NDM-1) gene were investigated in 6 Escherichia coli, 7 Klebsiella pneumoniae, 1 Citrobacter freundii, 1 Proteus mirabilis, and 1 Providencia stuartii isolate of worldwide origin. Clonal diversity was observed for both E. coli and K. pneumoniae. The bla(NDM-1) gene was carried by different plasmid types (IncA/C, IncF, IncL/M, or untypeable) and was likely chromosome borne in two isolates. The bla(NDM-1) plasmids coharbored a variety of resistance determinants, including β-lactamase genes, quinolone resistance genes, and 16S RNA methylase genes.
  Antimicrobial Agents and Chemotherapy 08/2011; 55(11):5403-7. · 4.84 Impact Factor
• Article: Emergence of OXA-48-producing Escherichia coli clone ST38 in France.

  Laurent Poirel, Sandrine Bernabeu, Nicolas Fortineau, Isabelle Podglajen, Christine Lawrence, Patrice Nordmann
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  ABSTRACT: The bla(OXA-48) gene encodes a carbapenem-hydrolyzing class D ß-lactamase that was first identified in Klebsiella pneumoniae from Turkey in 2003 and then extensively in that country (1, 11).…
  Antimicrobial Agents and Chemotherapy 07/2011; 55(10):4937-8. · 4.84 Impact Factor
• Article: Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland.

  Laurent Poirel, Jacques Schrenzel, Abdessalam Cherkaoui, Sandrine Bernabeu, Gesuele Renzi, Patrice Nordmann
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  ABSTRACT: To analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in Geneva University Hospitals, Switzerland. PCR and sequencing were used to identify β-lactamases, 16S RNA methylases and plasmid-mediated quinolone resistance genes. The transferable properties of the plasmids were analysed, as well as their plasmid type. The strains were typed by multilocus sequence typing. Three patients were found to be positive for NDM-1-producing enterobacterial isolates (one with Escherichia coli and Klebsiella pneumoniae, one with K. pneumoniae only and one with Proteus mirabilis), where NDM-1 stands for New Delhi metallo-β-lactamase-1. The bla(NDM-1) carbapenemase gene was detected in all isolates in addition to genes encoding narrow-spectrum β-lactamases (TEM-1, SHV-11, OXA-1, OXA-9 and OXA-10), extended-spectrum β-lactamases (CTX-M-15, CMY-16 and CMY-30), ArmA and quinolone resistance determinants (Qnr). The bla(NDM-1) gene was located on conjugative IncA/C- or IncF-type plasmids. Upstream of the bla(NDM-1) gene, part of ISAba125, previously identified in NDM-1-negative Acinetobacter baumannii, was found. Downstream of the bla(NDM-1) gene, variable sequences were found. This work constitutes the first identification of NDM-1 producers in Switzerland. Interestingly, patients from whom these NDM-1-producing isolates were recovered had a link with the Indian subcontinent or the Balkans.
  Journal of Antimicrobial Chemotherapy 05/2011; 66(8):1730-3. · 5.07 Impact Factor
• Article: Detection of NDM-1-producing Klebsiella pneumoniae in Kenya.

  Laurent Poirel, Gunturu Revathi, Sandrine Bernabeu, Patrice Nordmann
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  ABSTRACT: Seven carbapenem-resistant NDM-1-positive Klebsiella pneumoniae isolates were recovered from patients hospitalized between 2007 and 2009 in different wards at a referral and tertiary care center in Nairobi. Most of the isolates were obtained from urine. All isolates carried the bla(NDM-1) carbapenemase gene previously reported from India, Pakistan, and the United Kingdom. These isolates were clonally related and expressed many other resistance determinants, including β-lactamases CTX-M-15, OXA-1, OXA-9, CMY-6, and aminoglycoside resistance methylase RmtC. This work corresponds to the first report of NDM-1 producers in Africa.
  Antimicrobial Agents and Chemotherapy 02/2011; 55(2):934-6. · 4.84 Impact Factor
• Article: Global spread of New Delhi metallo-β-lactamase 1.

  Laurent Poirel, Cécile Hombrouck-Alet, Claire Freneaux, Sandrine Bernabeu, Patrice Nordmann
  The Lancet Infectious Diseases 12/2010; 10(12):832. · 17.39 Impact Factor
• Article: NDM-1-producing Klebsiella pneumoniae isolated in the Sultanate of Oman.

  Laurent Poirel, Zaina Al Maskari, Fatma Al Rashdi, Sandrine Bernabeu, Patrice Nordmann
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  ABSTRACT: To analyse the mechanisms responsible for multidrug resistance in two carbapenem-resistant Klebsiella pneumoniae isolates recovered from patients hospitalized in Oman. PCR and sequencing were used to search for β-lactamase and 16S RNA methylase genes. Multilocus sequence typing was used to determine the sequence type (ST) of each isolate. Clonal relationships were evaluated by PFGE. Both isolates carried the bla(NDM-1) carbapenemase gene. Isolate 601 was recovered from a patient who was transferred from India, whereas isolate 419 was from an Omani patient who had not travelled abroad. The two isolates were clonally unrelated, and belonged to ST14 (isolate 601) and ST340 (isolate 419). In addition to NDM-1, the ST14 isolate expressed β-lactamases CTX-M-15, SHV-28, OXA-1, OXA-9 and TEM-1, and the aminoglycoside resistance methylase ArmA. The ST340 isolate expressed β-lactamases SHV-11, OXA-1 and ArmA. In both isolates, the bla(NDM-1) gene was located on plasmids that were of similar size (170 kb), but of different incompatibility groups. This is the first description of NDM-1 producers in the Arabian peninsula and in the Middle East.
  Journal of Antimicrobial Chemotherapy 11/2010; 66(2):304-6. · 5.07 Impact Factor
• Article: Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India.

  Laurent Poirel, Alain Ros, Anne Carricajo, Philippe Berthelot, Bruno Pozzetto, Sandrine Bernabeu, Patrice Nordmann
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  ABSTRACT: The lastest threat in multidrug resistance in Gram negatives corresponds to the emergence of NDM-1-producing isolates that have been identified in several countries worldwide....
  Antimicrobial Agents and Chemotherapy 10/2010; 55(1):447-8. · 4.84 Impact Factor
• Article: A novel IncQ plasmid type harbouring a class 3 integron from Escherichia coli.

  Laurent Poirel, Alessandra Carattoli, Sandrine Bernabeu, Thomas Bruderer, Reno Frei, Patrice Nordmann
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  ABSTRACT: To determine the genetic structures at the origin of the mobilization of the extended-spectrum beta-lactamase (ESBL) bla(GES-1) gene in an Escherichia coli clinical isolate. ESBL-encoding genes and class 1 or class 3 integron-specific motifs were screened. Conjugation experiments were performed to determine whether the plasmid-carrying bla(GES-1) gene was self-transferable. Plasmid sequencing was achieved by a primer-walking approach. The bla(GES-1) gene was located in a class 3 integron. That unusual genetic structure was itself located on an approximately 9 kb plasmid, pQ7, which was not self-transferable. Sequence analysis revealed that plasmid pQ7 belonged to a novel subtype of the IncQ group. This study identified for the first time the bla(GES-1) gene in E. coli and in Switzerland. It describes a novel IncQ-type plasmid subgroup that possesses original features, in particular iteron sequences that constitute a hot spot for integration of foreign DNA.
  Journal of Antimicrobial Chemotherapy 08/2010; 65(8):1594-8. · 5.07 Impact Factor
• Article: Nosocomial spread of ESBL-positive Enterobacter cloacae co-expressing plasmid-mediated quinolone resistance Qnr determinants in one hospital in France.

  Anaïs Potron, Laurent Poirel, Sandrine Bernabeu, Xavier Monnet, Christian Richard, Patrice Nordmann
  Journal of Antimicrobial Chemotherapy 07/2009; 64(3):653-4. · 5.07 Impact Factor
• Source

  Available from: Reno Frei

  Article: Multidrug-resistant Providencia stuartii expressing extended-spectrum beta-lactamase PER-1, originating in Kosovo.

  Laurent Poirel, Thomas Bruderer, Reno Frei, Sandrine Bernabeu, Peter Graber, Patrice Nordmann
  Journal of Antimicrobial Chemotherapy 07/2008; 61(6):1392-3. · 5.07 Impact Factor
• Article: Multidrug-resistant Pseudomonas aeruginosa isolate co-expressing extended-spectrum beta-lactamase PER-1 and metallo-beta-lactamase VIM-2 from Turkey.

  Yusuf Yakupogullari, Laurent Poirel, Sandrine Bernabeu, Ahmet Kizirgil, Patrice Nordmann
  Journal of Antimicrobial Chemotherapy 02/2008; 61(1):221-2. · 5.07 Impact Factor
• Article: Multicopy blaOXA-58 gene as a source of high-level resistance to carbapenems in Acinetobacter baumannii.

  Alessia Bertini, Laurent Poirel, Sandrine Bernabeu, Daniela Fortini, Laura Villa, Patrice Nordmann, Alessandra Carattoli
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  ABSTRACT: The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 in a large University Hospital of Rome, Italy, were investigated. These isolates were related and possessed similar plasmids carrying the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable levels of resistance to carbapenems. Analysis of sequences surrounding the bla(OXA-58) gene showed genetic variability, with the presence in several isolates of multiple copies of the bla(OXA-58) gene; this extra copy number was likely related to an IS26-mediated transposition or recombination process.
  Antimicrobial Agents and Chemotherapy 08/2007; 51(7):2324-8. · 4.84 Impact Factor
• Article: SME-2-producing Serratia marcescens isolate from Switzerland.

  Laurent Poirel, Aline Wenger, Jacques Bille, Sandrine Bernabeu, Thierry Naas, Patrice Nordmann
  Antimicrobial Agents and Chemotherapy 07/2007; 51(6):2282-3. · 4.84 Impact Factor