Xiaohua Huang

Nanjing Normal University, Nan-ching, Jiangsu Sheng, China

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Publications (78)231.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Bisphenol A (BPA), an emerging pollutant in the environment, with potential toxic effects on plants; however, the toxicity mechanism remains largely unknown. The antioxidant system plays an important role in protecting plants against the damage of stress. Here, the effects of BPA on the antioxidant system [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbic acid (AsA), proline, reduced glutathione (GSH)], reactive oxygen species [ROS, hydrogen peroxide (H2 O2 ), superoxide anion (O2 (-) )] accumulation and membrane lipid peroxidation [malondialdehyde (MDA), cell membrane permeability] in soybean seedling roots were investigated. The 1.5 mg L(-1) BPA exposure didn't affect test indices in the roots. The exposure to 3.0, 6.0, 12.0 or 24.0 mg L(-1) BPA caused increases in the SOD (except for 3.0 mg L(-1) BPA) and CAT activities as well as the AsA, proline and GSH (except for 3.0 mg L(-1) BPA) contents, leading to increases in the H2 O2 and O2 (-) contents and to membrane lipid peroxidation. The 48.0 or 96.0 mg L(-1) exposure BPA caused decreases in the CAT activity and AsA/GSH content as well as increases in the SOD and POD activities and the proline content, leading to excess ROS accumulation (i.e. H2 O2 and O2 (-) ) and cell membrane damage. After withdrawal of BPA exposure, ROS accumulation and membrane lipid peroxidation were alleviated through regulating special antioxidant enzyme or substance. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Environmental Toxicology and Chemistry 02/2015; 34(5). DOI:10.1002/etc.2904 · 2.83 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) is a ubiquitous chemical in the environment and is potentially harmful to plants. However, relevant studies on the effects of BPA on plants are relatively scarce. In the present work, the effects of BPA on the biomass (fresh and dry weight), absorptive function (activity and absorptive area), and mineral element levels in soybean (Glycine max L.) seedling roots treated with 1.5 mg L-1, 3.0 mg L-1, 6.0 mg L-1, 12.0 mg L-1, 24.0 mg L-1, 48.0 mg L-1, and 96.0 mg L-1 BPA were investigated. Treatment with 1.5 mg L-1 BPA increased the levels of nitrate and other mineral elements (P, K, Mg, Mn, Zn, and Mo) in the roots, whereas treatments with BPA at higher concentrations decreased the levels of these elements in the roots. All treatments with BPA caused increases in the levels of ammonium, Ca, Fe, and Cu in the roots. Moreover, treatment with 1.5 mg L-1 BPA increased the fresh weight, dry weight, activity and absorptive area of the roots, whereas treatments with BPA at higher concentrations decreased these indices in a dose-dependent manner. Furthermore, correlation analysis data showed that BPA affected the levels of mineral elements and absorptive function of soybean seedling roots, which may be the physiological basis of BPA action on plants. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 01/2015; 34(1). DOI:10.1002/etc.2770 · 2.83 Impact Factor
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    ABSTRACT: In this paper, the novel poly(lactic-co-glycolic acid)-F127 nanospheres (PLGA-F127 NSs) were synthesized and used to establish an amperometric glucose biosensor that can be applied in whole blood directly. This property of glucose biosensor was based on the antibiofouling property of PLGAF127 NSs. More details of preparing PLGA-F127 NSs and immobilizing glucose oxidase (GOx) on (PLGA-F127)/glass carbon electrode (GCE) were presented. Then, the electrochemical behaviors of the biosensor in whole blood were studied. The cyclic voltammetric results indicated that GOx immobilized on PLGA-F127 NSs exhibited direct electron transfer reaction, which led to stable amperometric biosensing for glucose with a detection limit of 5.57×10−6 M (S/N = 3). The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their concentration normally encountered in blood. The development of materials science will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
    Journal of Nanoscience and Nanotechnology 01/2015; 15(1). DOI:10.1166/jnn.2015.8771 · 1.34 Impact Factor
  • Qing Yang, Lihong Wang, Qing Zhou, Xiaohua Huang
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    ABSTRACT: The environmental safety of rare earth elements (REEs), especially the toxic effect of REEs on plants, has attracted increasing attention. However, the cellular mechanism of this toxic effect remains largely unknown. Here, the toxic effects of heavy REE terbium ion [Tb(III)] on the cell membrane of horseradish roots were investigated by using electron microscope autoradiography (EMARG) and histochemical methods. The results indicated that Tb(III) was distributed in the extracellular and intracellular spaces of the roots after horseradish was treated with Tb(III). Moreover, the percentage contents of the unsaturated fatty acids in the membrane lipids, the current of the outward K+ channel and the average diameter of membrane proteins in the roots of horseradish treated with Tb(III) were decreased; on the contrary, the percentage contents of the saturated fatty acids and malondialdehyde in the roots of horseradish treated with Tb(III) were increased. Furthermore, the contents of intracellular N, P, Mg and Fe in the roots of horseradish treated with Tb(III) were decreased, while the contents of intracellular K and Ca in the roots of horseradish treated with Tb(III) were increased. Finally, the effects of Tb(III) on horseradish roots were increased with increasing concentration or duration of Tb(III) treatment. In conclusion, after horseradish was treated with Tb(III), Tb(III) could enter the cells of horseradish roots and lead to the toxic effects on horseradish, which caused the oxidation of the unsaturated fatty acids in the membrane lipids, the changes in the membrane proteins (including the outward K+ channel), the decrease in the membrane fluidity, and then the inhibition of the intracellular/extracellular-ion exchange in horseradish roots.
    Ecotoxicology and Environmental Safety 01/2015; 111:48–58. DOI:10.1016/j.ecoenv.2014.10.002 · 2.48 Impact Factor
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    ABSTRACT: Rare earth elements, especially terbium (Tb), are high-valence heavy metal elements that accumulate in the environment, and they show toxic effects on plants. Signaling molecules regulate many physiological and biochemical processes in plants. How rare earth elements affect signaling molecules remains largely unknown. In the present study, the effects of Tb(3+) on some extracellular and intracellular signaling molecules (gibberellic acid, abscisic acid, auxin, H2O2, and Ca(2+)) in horseradish leaves were investigated by using high-performance liquid chromatography, X-ray energy spectrometry, and transmission electron microscopy, and Tb(3+) was sprayed on the surface of leaves. Tb(3+) treatment decreased the auxin and gibberellic acid contents and increased the abscisic acid content. These changes in the contents of phytohormones (gibberellic acid, abscisic acid, and auxin) triggered excessive production of intracellular H2O2. Consequently, the increase in H2O2 content stimulated the influx of extracellular Ca(2+) and the release of Ca(2+) from Ca(2+) stores, leading to Ca(2+) overload and the resulting inhibition of physiological and biochemical processes. The effects outlined above were more evident with increasing the concentration of Tb(3+) sprayed on horseradish leaves. Our data provide a possible underlying mechanism of Tb(3+) action on plants.
    Biological Trace Element Research 12/2014; 164(1). DOI:10.1007/s12011-014-0209-z · 1.61 Impact Factor
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    ABSTRACT: Rare earth element pollution and elevated ultraviolet-B (UV-B) radiation occur simultaneously in some regions, but the combined effects of these two factors on plants have not attracted enough attention. Nitrogen nutrient is vital to plant growth. In this study, the combined effects of lanthanum(III) and elevated UV-B radiation on nitrate reduction and ammonia assimilation in soybean (Glycine max L.) roots were investigated. Treatment with 0.08 mmol L(-1) La(III) did not change the effects of elevated UV-B radiation on nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), nitrate, ammonium, amino acids, or soluble protein in the roots. Treatment with 0.24 mmol L(-1) La(III) and elevated UV-B radiation synergistically decreased the NR, NiR, GS, and GOGAT activities as well as the nitrate, amino acid, and soluble protein levels, except for the GDH activity and ammonium content. Combined treatment with 1.20 mmol L(-1) La(III) and elevated UV-B radiation produced severely deleterious effects on all test indices, and these effects were stronger than those induced by La(III) or elevated UV-B radiation treatment alone. Following the withdrawal of La(III) and elevated UV-B radiation, all test indices for the combined treatments with 0.08/0.24 mmol L(-1) La(III) and elevated UV-B radiation recovered to a certain extent, but they could not recover for treatments with 1.20 mmol L(-1) La(III) and elevated UV-B radiation. In summary, combined treatment with La(III) and elevated UV-B radiation seriously affected nitrogen nutrition in soybean roots through the inhibition of nitrate reduction and ammonia assimilation.
    Biological Trace Element Research 11/2014; 163(1-2). DOI:10.1007/s12011-014-0174-6 · 1.61 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) is ubiquitous in the environment due to its continual application in plastics and epoxy resin industry. Cadmium (Cd) is a highly toxic heavy metal element mainly used in smelting, electroplating and plastic/dye manufacturing. BPA and Cd pollution exist simultaneously in many agricultural regions. However, little information is available regarding the combined effects of BPA and Cd on plants. Here, the combined effects of BPA and Cd on the photosynthesis, chlorophyll fluorescence and content of soybean seedlings were investigated using non-invasive technology. Our data show that the combined treatment of 1.5 mg/L BPA and 0.2 mg/L Cd synergistically improved the net photosynthetic rate (Pn), initial fluorescence (F0), maximal photochemical efficiency (Fv/Fm), effective quantum yield of photosystem II (ΦPSII), photosynthetic electron transport rate (ETR) and chlorophyll content. Moreover, the combined treatment of 1.5 mg/L BPA and 3.0 mg/L Cd increased the F0 and decreased the Pn, Fv/Fm, ΦPSII and ETR, while BPA and Cd exhibited an antagonistic effect. Furthermore, the combined treatment of 17.2/50.0 mg/L BPA and 3.0/10.0 mg/L Cd synergistically decreased the Pn, Fv/Fm, ΦPSII, ETR and chlorophyll content, while it increased the F0. Finally, the effects of BPA and Cd on photosynthesis, chlorophyll fluorescence and chlorophyll content ceased when BPA stress was stopped. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 11/2014; 33(11). DOI:10.1002/etc.2720 · 2.83 Impact Factor
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    ABSTRACT: Bisphenol A (BPA), as an emerging environmental pollutant, is potentially harmful to plant growth. Chlorophyll (Chl) is critical in photosynthesis that provides matter and energy for plant growth. How BPA affects the chlorophyll content remains largely unknown. Here, the effects of BPA on Chl synthesis in soybean seedlings were investigated. Exposure to 1.5 mg/L BPA decreased the 5-aminolevulinic acid (ALA) content and increased protoporphyrin IX (Proto IX), magnesium protoporphyrin, and protochlorophyll contents and 5-aminolaevulinic acid dehydratase, porphobilinogen deaminase, uroporphyrinogen III synthase, uroporphyrinogen III decarboxylase, and protoporphyrinogen oxidase activities. Exposure to 17.2 and 50.0 mg/L BPA exerted the opposite effects on these four intermediates and five enzymes. Following the withdrawal of BPA exposure, the aforementioned parameters gradually recovered, except magnesium protoporphyrin content in exposure to 50.0 mg/L BPA. Our findings revealed that exposure to low-concentration BPA increased the Chl content in soybean seedlings through improving Chl synthesis, especially the conversion from ALA to Proto IX, whereas exposure to high-concentration BPA decreased the Chl content through inhibiting Chl synthesis, especially the conversion from ALA to Proto IX. The dual effects of BPA were largely reversed following the withdrawal of BPA exposure.
    Environmental Science and Pollution Research 10/2014; 22(8). DOI:10.1007/s11356-014-3764-0 · 2.76 Impact Factor
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    ABSTRACT: We prepared poly-L-aspartic acid (PASP) functionalized NaYF4:Yb3+,Er3+ upconversion nanoparticles (UCNP-PASP). These nanoparticles can give red upconversion emission under the excitation at 915 nm, whose wavelength of the emission and excitation located in the optical window of biological tissue. Dynamic laser scatting and zeta potentials of UCNP-PASP were used to study their stabilities in different aqueous solution. To understand the mechanism of the red emission of UCNP-PASP, photo-luminescence spectrum of samples was recorded before and after modification with PASP, poly acrylic acid (PAA) and polyetherimide (PEI) ligand under the excitation at 915 nm and 980 nm, respectively. The cytotoxicity of the UCNP-PASP was also examined on A549 cell and KB cell by MTT assay. And more, the PASP-functionalized UCNP was employed as the potential biomarker for in vitro and in vivo experiments of upconversion luminescence imaging.
    ACS Applied Materials & Interfaces 10/2014; 6(20). DOI:10.1021/am5057272 · 5.90 Impact Factor
  • Lihong Wang, Wen Wang, Qing Zhou, Xiaohua Huang
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    ABSTRACT: Rare earth elements (REEs) pollution and acid rain are environmental issues, and their deleterious effects on plants attract worldwide attention. These two issues exist simultaneously in many regions, especially in some rice-growing areas. However, little is known about the combined effects of REEs and acid rain on plants. Here, the combined effects of lanthanum chloride (LaCl3), one type of REE salt, and acid rain on photosynthesis in rice were investigated. We showed that the combined treatment of 81.6μM LaCl3 and acid rain at pH 4.5 increased net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO2 concentration (Ci), Hill reaction activity (HRA), apparent quantum yield (AQY) and carboxylation efficiency (CE) in rice. The combined treatment of 81.6μM LaCl3 and acid rain at pH 3.5 began to behave toxic effects on photosynthesis (decreasing Pn, Gs, HRA, AQY and CE, and increasing Ci), and the maximally toxic effects were observed in the combined treatment of 2449.0μM LaCl3 and acid rain at pH 2.5. Moreover, the combined effects of LaCl3 and acid rain on photosynthesis in rice depended on the growth stage of rice, with the maximal effects occurring at the booting stage. Furthermore, the combined treatment of high-concentration LaCl3 and low-pH acid rain had more serious effects on photosynthesis in rice than LaCl3 or acid rain treatment alone. Finally, the combined effect of LaCl3 and acid rain on Pn in rice resulted from the changes in stomatic (Gs, Ci) and non-stomatic (HRA, AQY and CE) factors.
    Chemosphere 10/2014; 112C:355-361. DOI:10.1016/j.chemosphere.2014.04.069 · 3.50 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) and cadmium (Cd) pollution exist simultaneously in many regions. However, little information is available regarding the combined effects of BPA and Cd pollution on plants. Plant roots are in direct contact with the soil, which is an important compartment of BPA and Cd. Here, the effects of combined BPA and Cd pollution on soybean seedling roots were evaluated in pot experiments. The combined treatment with BPA and Cd at the low concentrations (1.5 mg/kg BPA and 0.2 mg/kg Cd) improved soybean seedling root growth. However, the other combined BPA and Cd treatments, including the combined treatments with BPA (Cd) at the low concentration and Cd (BPA) at the high concentration, as well as the combined treatment with BPA and Cd at the high concentrations inhibited soybean seedling root growth. The improvement or inhibition of soybean seedling root growth was greater in the combined BPA and Cd treatments than in the single treatments. The effects of the combined BPA and Cd treatments on root growth resulted from changes in nitrate assimilation. In addition, the combined effects of BPA and Cd on the nitrate and ammonium contents in roots are also discussed. This research provides a basic understanding of the combined effects of BPA and Cd pollution on plant roots. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 09/2014; 33(9). DOI:10.1002/etc.2647 · 2.83 Impact Factor
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    ABSTRACT: Chemiluminescence was used as an excitation light source to construct a universal photoelectrochemical platform based on Mn2+-doped NaYF4:Yb/Er upconversion nanoparticles, which greatly improves the electrochemiluminescence intensity and offers more stable cathodic signals compared to pure NaYF4:Yb/Er NPs. Here, we report for the first time the ECL behaviors of Mn2+-doped NaYF4:Yb/Er NPs, which were synthesized via a facile strategy. Mn2+ doping resulted in a 4-fold ECL intensity enhancement of NaYF4:Yb/Er. The characteristics of Mn2+-doped NaYF4:Yb/Er nanocomposites were obtained using transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDS), and fluorescence spectra. After all the results had indicated that NaYF4:Yb/Er upconversion nanoparticles were successfully doped with Mn2+, the electrochemiluminescence platform was built. The as-prepared NaYF4:Yb/Er NPs were rendered water-soluble and then employed as ECL emitters for carcinoembryonic antigen (CEA) determination. The results indicated that the modified electrode can be used to determine CEA without interference from non-specific proteins, with a low detection limit of 5.2 pg mL−1. The biosensor can also be used for quantification of the CEA concentration in real samples.
    08/2014; 2(38):6626-6633. DOI:10.1039/C4TB00717D
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    ABSTRACT: In this work, three kinds of nanostructured silica-phytic acid (SiO2-PA) materials with diverse morphologies including spherical SiO2-PA (s-SiO2-PA), rod-like (r-SiO2-PA), and helical SiO2-PA (h-SiO2-PA) were prepared with the help of electrostatic interaction. The SiO2-PA nanomaterials with different morphologies were characterized by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR), electrochemical impedance spectroscopy (EIS) and circular dichroism spectrum (CD). Diverse morphologies of SiO2-PA were used as electrode decorated materials to achieve a high efficiency for electrochemical dopamine (DA) detection. The laccase biosensors were fabricated by immobilizing different morphologies of SiO2-PA nanomaterials and laccase onto the glassy carbon electrode (GCE) surface, successively. Then the electrochemical responses of the different morphologies of nanostructured SiO2-PA nanomaterials to laccase were discussed. Results indicated that compared to laccase/s-SiO2-PA and laccase/r-SiO2-PA, the laccase/h-SiO2-PA modified electrode showed the best electrochemical performances.
    Langmuir 08/2014; 30(37). DOI:10.1021/la503104x · 4.38 Impact Factor
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    ABSTRACT: Dual-modality imaging with magnetic resonance (MR) and upconversion luminescence (UCL) is a promising technique for molecular imaging in biomedical research. Multifunctional lanthanide-based nanoparticles have been widely investigated as agents for contrast enhanced MR and fluorescence imaging. However, the use of rare earth fluoride nanoparticles for dual-modality imaging of T2-weighted MR and UCL is rarely reported. We find that NaYF4:Yb(3+),Tm(3+),Co(2+) (MUC) nanorods can be applied as a high-performance dual contrast agent for both T2-weighted MR and UCL dual-modality imaging. After modification with 6-O-carboxymethyl chitosan (OCC), MUC nanorods can be endocytosed by cells without showing signs of cytotoxicity. High-quality UCL images of living cells incubated with MUC-OCC nanorods were acquired on a near-infrared (NIR) confocal microscopy under the excitation at 980 nm. Moreover, MUC-OCC nanorods display high transverse (r2) relaxivities in vitro. The application of low-dose MUC-OCC nanorods for NIR-to-NIR UCL and MR dual-modality in vivo imaging was also carried out successfully. In addition, the toxicity of MUC-OCC nanorods was evaluated by MTT assay, serological tests and histological analysis of visceral organs.
    Biomaterials 08/2014; DOI:10.1016/j.biomaterials.2014.07.031 · 8.31 Impact Factor
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    ABSTRACT: To explore how lead (Pb) and acid rain simultaneously affect plants, the combined effects of Pb and acid rain on the chlorophyll content, chlorophyll fluorescence reaction, Hill reaction rate, and Mg(2+)-ATPase activity in soybean seedlings were investigated. The results indicated that, when soybean seedlings were treated with Pb or acid rain alone, the chlorophyll content, Hill reaction rate, Mg(2+)-ATPase activity, and maximal photochemical efficiency (F v/F m) were decreased, while the initial fluorescence (F 0) and maximum quantum yield (Y) were increased, compared with those of the control. The combined treatment with Pb and acid rain decreased the chlorophyll content, Hill reaction rate, Mg(2+)-ATPase activity, F v/F m, and Y and increased F 0 in soybean seedlings. Under the combined treatment with Pb and acid rain, the two factors showed additive effects on the chlorophyll content in soybean seedlings and exhibited antagonistic effects on the Hill reaction rate. Under the combined treatment with high-concentration Pb and acid rain, the two factors exhibited synergistic effects on the Mg(2+)-ATPase activity, F 0, F v/F m, as well as Y. In summary, the inhibition of the photosynthetic process is an important physiological basis for the simultaneous actions of Pb and acid rain in soybean seedlings.
    Biological Trace Element Research 07/2014; 161(1). DOI:10.1007/s12011-014-0088-3 · 1.61 Impact Factor
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    ABSTRACT: The development of novel nanomaterials for the ultra-sensitive detection of biological species has received great attention. Herein we report the design and synthesis of phytic acid functionalized silica nanoparticles (SiO2-PA NPs) and their biomedical sensor application for sensitive detection of dopamine. The SiO2-PA NPs were characterized by transmission electron microscopy (TEM) and Energy dispersive X-ray spectrometer (EDS). The biocompatibility between SiO2-PA NPs and laccase was also investigated by circular dichroism (CD) spectra. Moreover, the novel laccase biosensor based on SiO2-PA NPs for sensitive detection of dopamine (DA) was fabricated, and the electrochemical properties of this laccase biosensor were investigated. The results showed the biosensor had good electrocatalytic activity toward DA with a wide linear range (0.99–103.10 μM) and a low detection limit 0.26 ± 0.003 μM. It can be attributed to the good biocompatibility and nano-effect of SiO2-PA NPs. The biosensor was successfully tested for determination of DA in pharmaceutical and rabbit blood serum samples. The development of biomimetic materials science will offer a novel platform for application to substance detection.
    Sensors and Actuators B Chemical 07/2014; 197:292–299. DOI:10.1016/j.snb.2014.03.002 · 3.84 Impact Factor
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    ABSTRACT: Hypocrellin A (HA) is a natural perylene quinonoid drug, which can kill tumor cells efficiently, so, based on this theory, a graphene and HA modified glassy carbon electrode was fabricated in this paper, and the interaction between HA and telomere DNA was investigated in pH 6 phosphate buffer solution by electrochemical methods for the first time. Thermodynamic study indicated that the binding is mainly van der Waals and hydrogen bond. The fluorescence experiment showed that the binding ratio $(n)$ of HA and telomere DNA were found to be 1.04 at 293 K, 0.71 at 298 K, 0.68 at 308 K, and 0.61 at 310 K, respectively. Under the optimized conditions, the oxidation current of HA was proportional to human telomere DNA concentration in the range from $5.91times 10^{-9}$ to $7.27times 10^{-7}~{rm mol}/{rm L}$ , with the correlation coefficient of 0.9920 and the detection limit of $1.21times 10^{-10}~{rm mol}/{rm L}~(S/N=3)$ . The proposed method was further applied to determine DNA in blood serum and abdominal fluid samples with satisfactory results.
    IEEE Sensors Journal 06/2014; 14(6):1904-1913. DOI:10.1109/JSEN.2014.2303114 · 1.85 Impact Factor
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    ABSTRACT: The enhanced ultraviolet-B (UV-B) radiation caused by ozone depletion may exert deleterious effects on plants. Therefore, studies on the effect of UV-B radiation on plants, as well as studies on the methods for alleviating the deleterious effects by chemical control, are of great significance. In this study, after soybean (Glycine max) seedlings were exposed to UV-B radiation (10.2 and 13.8 kJ m−2 day−1) for 5 days and the followed 6 days of restoration, respectively, the effects of 20 mg L−1 lanthanum (III) [La(III)] on leaf phenotype, photosynthetic rate, and production of ethylene and reactive oxygen species (ROS) were investigated. The results indicated that the exposure to 10.2 and 13.8 kJ m−2 day−1 UV-B radiation could cause injury to the leaf phenotype, and lead to the decrease in the content of chlorophyll and the net photosynthetic rate, and the increase in the contents of ROS, ethylene and 1-aminocyclopropanecarboxylic acid, and 1-aminocyclopropanecarboxylic acid synthase activity in soybean seedlings. Following the withdrawal of the enhanced UV-B radiation, the above mentioned parameters gradually recovered, and the recovery of soybean seedlings exposed to 10.2 kJ m−2 day−1 UV-B radiation was faster than those in soybean seedlings exposed to 13.8 kJ m−2 day−1 UV-B radiation. The leaf injury and the changes in the above indices that were induced by the enhanced UV-B radiation, especially at 10.2 kJ m−2 day−1, were alleviated after the pretreatment of soybean seedlings with 20 mg L−1 La(III). The results of the correlation analysis demonstrated that the injury to the leaf phenotype and the decrease in the photosynthetic rate of soybean seedlings were correlated with the increase in the ROS content that was induced by ethylene in soybean seedlings. The pretreatment with 20 mg L−1 La(III) alleviated the injury caused by the enhanced UV-B radiation through the regulation of the ROS production.
    Ecotoxicology and Environmental Safety 06/2014; 104:152–159. DOI:10.1016/j.ecoenv.2014.02.026 · 2.48 Impact Factor
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    ABSTRACT: Soil acidification and lead (Pb) pollution seriously affect the antioxidant system in plants. However, studies on their combined effects on the antioxidant system in plant roots are limited. Here, the combined effects of soil acidification and Pb 2+ on peroxidase (POD) activity, catalase (CAT) activity, malonydialdehyde content, cell membrane permeability and Pb content in soybean roots at different growth stages were investigated. We showed that at the seedling stage, combined treatment with soil acidification at pH 4.5/3.5 and 0.3 mM Pb 2+ increased POD activity, and combined treatment with soil acidification at pH 3.0 and 0.3/0.9 mM Pb 2+ increased CAT activity. Combined treatment with soil acidification at pH 4.5 and 0.9 mM Pb 2+ also increased CAT activity at the flowering and pod-formation stage. The remaining combined treatments decreased the POD activity, CAT activity and malonydialdehyde content, but increased the membrane permeability in the roots. Moreover, the combined effects of soil acidification and Pb 2+ on the antioxidant system in soybean roots at the seedling stage were stronger than those at the flowering/pod-formation stage and seed-filling stages. Furthermore, the combined effects on the antioxidant system resulted from the accumulation of Pb in the roots.
    Chemistry and Ecology 04/2014; 31(2):123-133. DOI:10.1080/02757540.2014.917176 · 1.18 Impact Factor
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    ABSTRACT: In order to probe into the enzymological mechanism for the regulation of lanthanum chloride (LaCl3) on flavonoid synthesis in plants under enhanced ultraviolet-B (UV-B) radiation, the effects of LaCl3 (20 and 60 mg l(-1)) on the content of flavonoids as well as the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate : coenzyme A ligase (4CL), and chalcone synthase (CHS) in soybean seedlings under enhanced UV-B radiation (2.6 and 6.2 kJ m(-2) day(-1)) were investigated. Enhanced UV-B radiation (2.6 and 6.2 kJ m(-2) day(-1)) caused the increase in the content of flavonoids as well as the activities of PAL, C4H, 4CL, and CHS in soybean seedlings. The treatment of 20 mg l(-1) LaCl3 also efficiently increased these indices, which promoted the flavonoid synthesis and provided protective effects for resisting enhanced UV-B radiation. On the contrary, the treatment of 60 mg l(-1) LaCl3 decreased the content of flavonoids as well as the activities of C4H, 4CL, and CHS in soybean seedlings except increasing the activity of PAL, which were not beneficial to the flavonoid synthesis and provided negative effects for resisting enhanced UV-B radiation. In conclusion, enhanced UV-B radiation caused the increase in the flavonoid synthesis by promoting the activities of PAL, C4H, 4CL, and CHS in soybean seedlings. The treatment of LaCl3 could change flavonoid synthesis in soybean seedlings under enhanced UV-B radiation by regulating the activities of PAL, C4H, 4CL, and CHS, which is an enzymological mechanism for the regulation of LaCl3 on flavonoid synthesis in plants under enhanced UV-B radiation.
    Environmental Science and Pollution Research 04/2014; 21(14). DOI:10.1007/s11356-014-2815-x · 2.76 Impact Factor

Publication Stats

480 Citations
231.77 Total Impact Points

Institutions

  • 2006–2015
    • Nanjing Normal University
      • • College of Chemistry and Materials Science
      • • College of Chemistry and Environmental Science
      • • Department of Chemistry
      Nan-ching, Jiangsu Sheng, China
  • 2012–2014
    • Jiangnan University
      • School of Environmental and Civil Engineering
      Wu-hsi, Jiangsu Sheng, China
  • 2003
    • Yangtze University
      Hu-pei-ts’un, Shanxi Sheng, China