Xiaohua Huang

Nanjing Normal University, Nan-ching, Jiangsu Sheng, China

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Publications (72)159.59 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this paper, the novel poly(lactic-co-glycolic acid)-F127 nanospheres (PLGA-F127 NSs) were synthesized and used to establish an amperometric glucose biosensor that can be applied in whole blood directly. This property of glucose biosensor was based on the antibiofouling property of PLGAF127 NSs. More details of preparing PLGA-F127 NSs and immobilizing glucose oxidase (GOx) on (PLGA-F127)/glass carbon electrode (GCE) were presented. Then, the electrochemical behaviors of the biosensor in whole blood were studied. The cyclic voltammetric results indicated that GOx immobilized on PLGA-F127 NSs exhibited direct electron transfer reaction, which led to stable amperometric biosensing for glucose with a detection limit of 5.57×10−6 M (S/N = 3). The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their concentration normally encountered in blood. The development of materials science will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
    Journal of Nanoscience and Nanotechnology 01/2015; 15(1). · 1.15 Impact Factor
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    ABSTRACT: We prepared poly-L-aspartic acid (PASP) functionalized NaYF4:Yb3+,Er3+ upconversion nanoparticles (UCNP-PASP). These nanoparticles can give red upconversion emission under the excitation at 915 nm, whose wavelength of the emission and excitation located in the optical window of biological tissue. Dynamic laser scatting and zeta potentials of UCNP-PASP were used to study their stabilities in different aqueous solution. To understand the mechanism of the red emission of UCNP-PASP, photo-luminescence spectrum of samples was recorded before and after modification with PASP, poly acrylic acid (PAA) and polyetherimide (PEI) ligand under the excitation at 915 nm and 980 nm, respectively. The cytotoxicity of the UCNP-PASP was also examined on A549 cell and KB cell by MTT assay. And more, the PASP-functionalized UCNP was employed as the potential biomarker for in vitro and in vivo experiments of upconversion luminescence imaging.
    ACS Applied Materials & Interfaces 10/2014; · 5.90 Impact Factor
  • Lihong Wang, Wen Wang, Qing Zhou, Xiaohua Huang
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    ABSTRACT: Rare earth elements (REEs) pollution and acid rain are environmental issues, and their deleterious effects on plants attract worldwide attention. These two issues exist simultaneously in many regions, especially in some rice-growing areas. However, little is known about the combined effects of REEs and acid rain on plants. Here, the combined effects of lanthanum chloride (LaCl3), one type of REE salt, and acid rain on photosynthesis in rice were investigated. We showed that the combined treatment of 81.6μM LaCl3 and acid rain at pH 4.5 increased net photosynthetic rate (Pn), stomatic conductance (Gs), intercellular CO2 concentration (Ci), Hill reaction activity (HRA), apparent quantum yield (AQY) and carboxylation efficiency (CE) in rice. The combined treatment of 81.6μM LaCl3 and acid rain at pH 3.5 began to behave toxic effects on photosynthesis (decreasing Pn, Gs, HRA, AQY and CE, and increasing Ci), and the maximally toxic effects were observed in the combined treatment of 2449.0μM LaCl3 and acid rain at pH 2.5. Moreover, the combined effects of LaCl3 and acid rain on photosynthesis in rice depended on the growth stage of rice, with the maximal effects occurring at the booting stage. Furthermore, the combined treatment of high-concentration LaCl3 and low-pH acid rain had more serious effects on photosynthesis in rice than LaCl3 or acid rain treatment alone. Finally, the combined effect of LaCl3 and acid rain on Pn in rice resulted from the changes in stomatic (Gs, Ci) and non-stomatic (HRA, AQY and CE) factors.
    Chemosphere 10/2014; 112C:355-361. · 3.14 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) is a ubiquitous chemical in the environment and is potentially harmful to plants. However, relevant studies on the effects of BPA on plants are relatively scarce. In the present work, the effects of BPA on the biomass (fresh and dry weight), absorptive function (activity and absorptive area), and mineral element levels in soybean (Glycine max L.) seedling roots treated with 1.5 mg L-1, 3.0 mg L-1, 6.0 mg L-1, 12.0 mg L-1, 24.0 mg L-1, 48.0 mg L-1, and 96.0 mg L-1 BPA were investigated. Treatment with 1.5 mg L-1 BPA increased the levels of nitrate and other mineral elements (P, K, Mg, Mn, Zn, and Mo) in the roots, whereas treatments with BPA at higher concentrations decreased the levels of these elements in the roots. All treatments with BPA caused increases in the levels of ammonium, Ca, Fe, and Cu in the roots. Moreover, treatment with 1.5 mg L-1 BPA increased the fresh weight, dry weight, activity and absorptive area of the roots, whereas treatments with BPA at higher concentrations decreased these indices in a dose-dependent manner. Furthermore, correlation analysis data showed that BPA affected the levels of mineral elements and absorptive function of soybean seedling roots, which may be the physiological basis of BPA action on plants. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 10/2014; · 2.62 Impact Factor
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    ABSTRACT: In this work, three kinds of nanostructured silica-phytic acid (SiO2-PA) materials with diverse morphologies including spherical SiO2-PA (s-SiO2-PA), rod-like (r-SiO2-PA), and helical SiO2-PA (h-SiO2-PA) were prepared with the help of electrostatic interaction. The SiO2-PA nanomaterials with different morphologies were characterized by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR), electrochemical impedance spectroscopy (EIS) and circular dichroism spectrum (CD). Diverse morphologies of SiO2-PA were used as electrode decorated materials to achieve a high efficiency for electrochemical dopamine (DA) detection. The laccase biosensors were fabricated by immobilizing different morphologies of SiO2-PA nanomaterials and laccase onto the glassy carbon electrode (GCE) surface, successively. Then the electrochemical responses of the different morphologies of nanostructured SiO2-PA nanomaterials to laccase were discussed. Results indicated that compared to laccase/s-SiO2-PA and laccase/r-SiO2-PA, the laccase/h-SiO2-PA modified electrode showed the best electrochemical performances.
    Langmuir : the ACS journal of surfaces and colloids. 08/2014;
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    ABSTRACT: Dual-modality imaging with magnetic resonance (MR) and upconversion luminescence (UCL) is a promising technique for molecular imaging in biomedical research. Multifunctional lanthanide-based nanoparticles have been widely investigated as agents for contrast enhanced MR and fluorescence imaging. However, the use of rare earth fluoride nanoparticles for dual-modality imaging of T2-weighted MR and UCL is rarely reported. We find that NaYF4:Yb(3+),Tm(3+),Co(2+) (MUC) nanorods can be applied as a high-performance dual contrast agent for both T2-weighted MR and UCL dual-modality imaging. After modification with 6-O-carboxymethyl chitosan (OCC), MUC nanorods can be endocytosed by cells without showing signs of cytotoxicity. High-quality UCL images of living cells incubated with MUC-OCC nanorods were acquired on a near-infrared (NIR) confocal microscopy under the excitation at 980 nm. Moreover, MUC-OCC nanorods display high transverse (r2) relaxivities in vitro. The application of low-dose MUC-OCC nanorods for NIR-to-NIR UCL and MR dual-modality in vivo imaging was also carried out successfully. In addition, the toxicity of MUC-OCC nanorods was evaluated by MTT assay, serological tests and histological analysis of visceral organs.
    Biomaterials 08/2014; · 8.31 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) is ubiquitous in the environment due to its continual application in plastics and epoxy resin industry. Cadmium (Cd) is a highly toxic heavy metal element mainly used in smelting, electroplating and plastic/dye manufacturing. BPA and Cd pollution exist simultaneously in many agricultural regions. However, little information is available regarding the combined effects of BPA and Cd on plants. Here, the combined effects of BPA and Cd on the photosynthesis, chlorophyll fluorescence and content of soybean seedlings were investigated using non-invasive technology. Our data show that the combined treatment of 1.5 mg/L BPA and 0.2 mg/L Cd synergistically improved the net photosynthetic rate (Pn), initial fluorescence (F0), maximal photochemical efficiency (Fv/Fm), effective quantum yield of photosystem II (ΦPSII), photosynthetic electron transport rate (ETR) and chlorophyll content. Moreover, the combined treatment of 1.5 mg/L BPA and 3.0 mg/L Cd increased the F0 and decreased the Pn, Fv/Fm, ΦPSII and ETR, while BPA and Cd exhibited an antagonistic effect. Furthermore, the combined treatment of 17.2/50.0 mg/L BPA and 3.0/10.0 mg/L Cd synergistically decreased the Pn, Fv/Fm, ΦPSII, ETR and chlorophyll content, while it increased the F0. Finally, the effects of BPA and Cd on photosynthesis, chlorophyll fluorescence and chlorophyll content ceased when BPA stress was stopped. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 08/2014; · 2.62 Impact Factor
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    ABSTRACT: To explore how lead (Pb) and acid rain simultaneously affect plants, the combined effects of Pb and acid rain on the chlorophyll content, chlorophyll fluorescence reaction, Hill reaction rate, and Mg(2+)-ATPase activity in soybean seedlings were investigated. The results indicated that, when soybean seedlings were treated with Pb or acid rain alone, the chlorophyll content, Hill reaction rate, Mg(2+)-ATPase activity, and maximal photochemical efficiency (F v/F m) were decreased, while the initial fluorescence (F 0) and maximum quantum yield (Y) were increased, compared with those of the control. The combined treatment with Pb and acid rain decreased the chlorophyll content, Hill reaction rate, Mg(2+)-ATPase activity, F v/F m, and Y and increased F 0 in soybean seedlings. Under the combined treatment with Pb and acid rain, the two factors showed additive effects on the chlorophyll content in soybean seedlings and exhibited antagonistic effects on the Hill reaction rate. Under the combined treatment with high-concentration Pb and acid rain, the two factors exhibited synergistic effects on the Mg(2+)-ATPase activity, F 0, F v/F m, as well as Y. In summary, the inhibition of the photosynthetic process is an important physiological basis for the simultaneous actions of Pb and acid rain in soybean seedlings.
    Biological Trace Element Research 07/2014; · 1.31 Impact Factor
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    ABSTRACT: Bisphenol A (BPA) and cadmium (Cd) pollution exist simultaneously in many regions. However, little information is available regarding the combined effects of BPA and Cd pollution on plants. Plant roots are in direct contact with the soil, which is an important compartment of BPA and Cd. Here, the effects of combined BPA and Cd pollution on soybean seedling roots were evaluated in pot experiments. The combined treatment with BPA and Cd at the low concentrations (1.5 mg/kg BPA and 0.2 mg/kg Cd) improved soybean seedling root growth. However, the other combined BPA and Cd treatments, including the combined treatments with BPA (Cd) at the low concentration and Cd (BPA) at the high concentration, as well as the combined treatment with BPA and Cd at the high concentrations inhibited soybean seedling root growth. The improvement or inhibition of soybean seedling root growth was greater in the combined BPA and Cd treatments than in the single treatments. The effects of the combined BPA and Cd treatments on root growth resulted from changes in nitrate assimilation. In addition, the combined effects of BPA and Cd on the nitrate and ammonium contents in roots are also discussed. This research provides a basic understanding of the combined effects of BPA and Cd pollution on plant roots. Environ Toxicol Chem © 2014 SETAC
    Environmental Toxicology and Chemistry 05/2014; · 2.62 Impact Factor
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    ABSTRACT: In order to probe into the enzymological mechanism for the regulation of lanthanum chloride (LaCl3) on flavonoid synthesis in plants under enhanced ultraviolet-B (UV-B) radiation, the effects of LaCl3 (20 and 60 mg l(-1)) on the content of flavonoids as well as the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate : coenzyme A ligase (4CL), and chalcone synthase (CHS) in soybean seedlings under enhanced UV-B radiation (2.6 and 6.2 kJ m(-2) day(-1)) were investigated. Enhanced UV-B radiation (2.6 and 6.2 kJ m(-2) day(-1)) caused the increase in the content of flavonoids as well as the activities of PAL, C4H, 4CL, and CHS in soybean seedlings. The treatment of 20 mg l(-1) LaCl3 also efficiently increased these indices, which promoted the flavonoid synthesis and provided protective effects for resisting enhanced UV-B radiation. On the contrary, the treatment of 60 mg l(-1) LaCl3 decreased the content of flavonoids as well as the activities of C4H, 4CL, and CHS in soybean seedlings except increasing the activity of PAL, which were not beneficial to the flavonoid synthesis and provided negative effects for resisting enhanced UV-B radiation. In conclusion, enhanced UV-B radiation caused the increase in the flavonoid synthesis by promoting the activities of PAL, C4H, 4CL, and CHS in soybean seedlings. The treatment of LaCl3 could change flavonoid synthesis in soybean seedlings under enhanced UV-B radiation by regulating the activities of PAL, C4H, 4CL, and CHS, which is an enzymological mechanism for the regulation of LaCl3 on flavonoid synthesis in plants under enhanced UV-B radiation.
    Environmental Science and Pollution Research 04/2014; · 2.76 Impact Factor
  • Lihong Wang, Qing Zhou, Xiaohua Huang
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    ABSTRACT: Rare earth elements (REEs) entering plant cells can directly interact with peroxidase in plants, which is the structural basis for the decrease in the activity of peroxidase. Different cellular compartments have different pH values. However, little information is available regarding the direct interaction between REEs and peroxidase in plants at different pH values. Here, we investigated the charge distribution on the surface of horseradish peroxidase (HRP) molecule as well as the interaction of terbium ion (Tb(3+), one type of REEs) and HRP at different pH values. Using the molecular dynamics simulation, we found that when the pH value was from 4.0 to 8.0, a large amount of negative charges were intensively distributed on the surface of HRP molecule, and thus, we speculated that Tb(3+) with positive charges might directly interact with HRP at pH 4.0-8.0. Subsequently, using ultraviolet-visible spectroscopy, we demonstrated that Tb(3+) could directly interact with HRP in the simulated physiological solution at pH 7.0 and did not interact with HRP in other solutions at pH 5.0, pH 6.0 and pH 8.0. In conclusion, we showed that the direct interaction between Tb(3+) and HRP molecule depended on the pH value of cellular compartments.
    Biological trace element research 01/2014; · 1.92 Impact Factor
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    ABSTRACT: The development of novel nanomaterials for the ultra-sensitive detection of biological species has received great attention. Herein we report the design and synthesis of phytic acid functionalized silica nanoparticles (SiO2-PA NPs) and their biomedical sensor application for sensitive detection of dopamine. The SiO2-PA NPs were characterized by transmission electron microscopy (TEM) and Energy dispersive X-ray spectrometer (EDS). The biocompatibility between SiO2-PA NPs and laccase was also investigated by circular dichroism (CD) spectra. Moreover, the novel laccase biosensor based on SiO2-PA NPs for sensitive detection of dopamine (DA) was fabricated, and the electrochemical properties of this laccase biosensor were investigated. The results showed the biosensor had good electrocatalytic activity toward DA with a wide linear range (0.99–103.10 μM) and a low detection limit 0.26 ± 0.003 μM. It can be attributed to the good biocompatibility and nano-effect of SiO2-PA NPs. The biosensor was successfully tested for determination of DA in pharmaceutical and rabbit blood serum samples. The development of biomimetic materials science will offer a novel platform for application to substance detection.
    Sensors and Actuators B Chemical 01/2014; 197:292–299. · 3.84 Impact Factor
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    ABSTRACT: The enhanced ultraviolet-B (UV-B) radiation caused by ozone depletion may exert deleterious effects on plants. Therefore, studies on the effect of UV-B radiation on plants, as well as studies on the methods for alleviating the deleterious effects by chemical control, are of great significance. In this study, after soybean (Glycine max) seedlings were exposed to UV-B radiation (10.2 and 13.8 kJ m−2 day−1) for 5 days and the followed 6 days of restoration, respectively, the effects of 20 mg L−1 lanthanum (III) [La(III)] on leaf phenotype, photosynthetic rate, and production of ethylene and reactive oxygen species (ROS) were investigated. The results indicated that the exposure to 10.2 and 13.8 kJ m−2 day−1 UV-B radiation could cause injury to the leaf phenotype, and lead to the decrease in the content of chlorophyll and the net photosynthetic rate, and the increase in the contents of ROS, ethylene and 1-aminocyclopropanecarboxylic acid, and 1-aminocyclopropanecarboxylic acid synthase activity in soybean seedlings. Following the withdrawal of the enhanced UV-B radiation, the above mentioned parameters gradually recovered, and the recovery of soybean seedlings exposed to 10.2 kJ m−2 day−1 UV-B radiation was faster than those in soybean seedlings exposed to 13.8 kJ m−2 day−1 UV-B radiation. The leaf injury and the changes in the above indices that were induced by the enhanced UV-B radiation, especially at 10.2 kJ m−2 day−1, were alleviated after the pretreatment of soybean seedlings with 20 mg L−1 La(III). The results of the correlation analysis demonstrated that the injury to the leaf phenotype and the decrease in the photosynthetic rate of soybean seedlings were correlated with the increase in the ROS content that was induced by ethylene in soybean seedlings. The pretreatment with 20 mg L−1 La(III) alleviated the injury caused by the enhanced UV-B radiation through the regulation of the ROS production.
    Ecotoxicology and Environmental Safety 01/2014; 104:152–159. · 2.20 Impact Factor
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    ABSTRACT: A novel hypoxanthine biosensor fabricated by immobilizing the xanthine oxidase (XOD) onto the phosphonic acid-functionalized silica (SiO2-P) film on the surface of glassy carbon electrode (GCE) was designed and constructed in this work. A biomimetic platform was designed with the phosphonic acid-functionalized silica nanoparticles (SiO2-P NPs) synthesized by the method of reverse microemulsion and electrostatic binding. In such a platform, XOD was selected as model protein to fabricate hypoxanthine biosensor based on SiO2-P NPs. The nanocomposite was characterized with transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDS) and electrochemical impedance spectroscopy (EIS). Based on the advantageous functions of SiO2-P NPs, the entrapped XOD could preserve its bioactivity and exhibited an excellent electrochemical behavior with a formal potential of -0.37V in phosphate buffer solution (PBS, pH=7). Response studies to hypoxanthine were carried out using current-time response curve. The biosensor exhibited a wide linear response ranging from 1.00×10(-6) to 2.61×10(-4)M. The detection limit of 2.33×10(-7)M at a signal-to-noise ratio of 3 was lower than that most reported previously. In addition, the electrode modified with XOD/(SiO2-P NPs) film also had a strong anti-interference ability in the presence of uric acid (UA) and ascorbic acid (AA). The assay results of hypoxanthine in fish samples were in a good agreement with the reference values.
    Talanta 12/2013; 117C:536-542. · 3.50 Impact Factor
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    ABSTRACT: Heavy metal pollution and soil acidification are serious global environmental issues. The combined pollution from acidification and heavy metal has become a new environmental issue in regions where the two issues simultaneously occur. However, studies on combined pollution are still limited. In the current study, we investigated the combined effect and mechanism of acidity and heavy metal [lead ion (Pb(2+))] on soybean biomass as well as on growth, nitrogen nutrition, and antioxidant system in soybean roots. Results showed that the combined treatment with acidity and Pb(2+) decreased the soybean biomass. At pH 4.5, the soybean biomass in the combined treatment with acidity and 0.9 mmol L(-1) Pb(2+) was lower than that in the combined treatment with acidity and Pb(2+) at 0.3 or 1.5 mmol L(-1). This result was also observed at pH 3.5 and 3.0. The combined treatment with acidity and Pb(2+) also resulted in the following consequences: root growth inhibition; decrease in nitrate, ammonium, and malondialdehyde contents; increase in nitrite reductase activity; and decrease in peroxidase activity. The extent at which the test indexes decreased/increased in the combined treatment was higher than that in the single acidity treatment. The correlation analysis results indicated that the decrease in the soybean biomass in the combined treatment with acidity and Pb(2+) resulted from the decrease in the root growth, nitrate-nitrogen assimilation, and peroxidase activity.
    Biological trace element research 09/2013; · 1.92 Impact Factor
  • Zhaoguo Sun, Lihong Wang, Qing Zhou, Xiaohua Huang
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    ABSTRACT: Rare earth pollution and acid rain pollution are both important environmental issues worldwide. In regions which simultaneously occur, the combined pollution of rare earth and acid rain becomes a new environmental issue, and the relevant research is rarely reported. Accordingly, we investigated the combined effects and mechanisms of lanthanum ion (La(3+)) and acid rain on the root phenotype of soybean seedlings. The combined pollution of low-concentration La(3+) and acid rain exerted deleterious effects on the phenotype and growth of roots, which were aggravated by the combined pollution of high-concentration La(3+) and acid rain. The deleterious effects of the combined pollution were stronger than those of single La(3+) or acid rain pollution. These stronger deleterious effects on the root phenotype and growth of roots were due to the increased disturbance of absorption and utilization of mineral nutrients in roots.
    Chemosphere 05/2013; · 3.14 Impact Factor
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    ABSTRACT: Quantification of the blood glucose concentration in the whole blood was not easy to achieve because the detection process was affected by many factors, such as glucose metabolism and biofouling. In this paper, we established an amperometric glucose biosensor applied in whole blood directly, which was based on the direct electron transfer of glucose oxidase (GOx) entrapped onto the Au-F127 nanospheres. Here, the Au-F127 nanospheres could provide a blood compatible surface with antifouling property for determination of glucose in whole blood. The cyclic voltammetric results indicated that GOx immobilized on the Au-F127 nanospheres exhibited direct electron transfer reaction, and the cyclic voltammogram (CV) displayed a pair of well-defined and nearly symmetric redox peaks with a formal potential of 93 mV. The biosensor had good electrocatalytic activity toward glucose with a low detection limit 3.15 pM. The glucose biosensor did not respond to ascorbic acid (AA) and uric acid (UA) at their high concentration encountered in blood. In this method, the biosensor was used for quantification of the concentration of glucose in whole blood samples. The data obtained from the biosensor showed good agreement with those from a biochemical analyzer in hospital.
    Journal of Biomedical Nanotechnology 05/2013; 9(5):825-32. · 7.58 Impact Factor
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    ABSTRACT: In this work, the silica- phytic acid (SiO(2)-PA) nanocomposites were synthesized by the method of reverse microemulsion and electrostatic binding. The newly designed materials were used to develop a novel glucose biosensor by immobilizing glucose oxidase (GOx) onto the SiO(2)-PA nanocomposites film on the surface of glassy carbon electrode (GCE). The characteristics of SiO(2)-PA nanocomposites and GOx were obtained by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and circular dichroism (CD) technique. All the results indicated that silica nanoparticales were modified with phosphate radicals successfully and the biomimetic surface was built. The entrapped GOx could preserve its bioactivity and exhibited an excellent electrochemical behavior with a formal potential of -0.548V in phosphate buffer solution (PBS) (pH=7). Response studies to glucose were carried out using differential pulse voltammetry (DPV). The results indicated that the modified electrode can be used to determine glucose without interference from l-ascorbic acid (AA) and uric acid (UA) with the low detection limit of 0.012mM. The comparison tests of DPVs of different electrodes in the absence and presence of glucose were also studied. The biosensor can also be used for quantification of the concentration of glucose in real samples.
    Biosensors & bioelectronics 01/2013; 44C:1-5. · 5.43 Impact Factor
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    ABSTRACT: The poly(N-isopropylacrylamide)-g-poly(N-isopropylacrylamide-co-styrene) microspheres (PNNS-MSs) were prepared by an emulsifier-free emulsion polymerization method. The blood compatibility of PNNS-MSs was characterized by in vitro for coagulation tests, hemolysis assay, plasma recalcification time, complement activation, platelet activation, and cytotoxicity experiments. The results showed that the PNNS-MSs have good blood compatibility and lack cytotoxicity, which may be attributed to the formation of a strong interfacial hydration layer that result from amphiphilic molecular structure of the PNIPAM shell and minimal interaction between PNNS-MSs interfaces and blood components. The PNNS-MSs provide a promising platform of blood circulation system for early illness diagnosis and therapy.
    Colloids and surfaces B: Biointerfaces 12/2012; 104C:61-65. · 4.28 Impact Factor
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    ABSTRACT: Positive and negative effects of rare earth elements (REEs) in life have been reported in many papers, but the cellular mechanisms have not been answered, especially the action sites of REEs on plasma membrane are unknown. Proteins on/in the plasma membrane perform main functions of the plasma membrane. Cerium (Ce) is the richest REEs in crust. Thus, the interaction between Ce(III) and the proteins on/in the plasma membrane, the morphology of protoplast, and the contents of nutrient elements in protoplast of horseradish were investigated using the optimized combination of the fluorescence microscopy, fluorescence spectroscopy, circular dichroism, scanning electron microscopy, and X-ray energy dispersive spectroscopy. It was found that Ce(III) at the low concentrations (10, 30 μM) could interact with proteins on/in the plasma membrane of horseradish, leading to the improvement in the structure of membrane proteins and the plasma membrane, which accelerated the intra-/extra-cellular substance exchange and further promoted the development of cells. When horseradish was treated with Ce(III) at the high concentrations (60, 80 μM), Ce(III) also could interact with the proteins on/in the plasma membrane of horseradish, leading to the destruction in the structure of membrane proteins and the plasma membrane. These effects decelerated the intra-/extra-cellular substance exchange and further inhibited the development of cells. Thus, the interaction between Ce(III) and proteins on/in the plasma membrane in plants was an important reason of the positive and negative effects of Ce(III) on plants. The results would provide some references for understanding the cellular effect mechanisms of REEs on plants.
    Biological trace element research 10/2012; · 1.92 Impact Factor

Publication Stats

204 Citations
159.59 Total Impact Points

Institutions

  • 2006–2014
    • Nanjing Normal University
      • • College of Chemistry and Materials Science
      • • College of Chemistry and Environmental Science
      • • Department of Chemistry
      Nan-ching, Jiangsu Sheng, China
  • 2007–2013
    • Jiangnan University
      • School of Environmental and Civil Engineering
      Wuxi, Jiangsu Sheng, China
  • 2003
    • Yangtze University
      Hu-pei-ts’un, Shanxi Sheng, China